Quantification of the Anti-diabetic Effect of Allium cepa.

BACKGROUND: Allium cepa, or onion, boosts numerous health benefits, including anti-diabetic effects. Its rich array of antioxidants and sulfur compounds not only aids heart health by lowering cholesterol and blood pressure but also exhibits anti-inflammatory properties. Onion's antibacterial and antiviral properties help combat infections, while its compounds like quercetin show promise in cancer prevention. Additionally, Allium cepa supports respiratory health by relieving coughs and colds and aids digestion with its prebiotic properties. Incorporating onions into a balanced diet can enhance overall well-being, including managing blood sugar levels in individuals with diabetes. AIM AND OBJECTIVE: This study aims to determine if the ethanolic extract from the dried peel of Allium cepa holds potential as an anti-diabetic agent, with a focus on its ability to manage diabetes and reduce blood sugar levels. METHODOLOGY: To prepare the ethanolic extract from dried onion peel, the peel was finely ground and soaked in ethanol. The mixture was then agitated and filtered to separate the liquid extract. Finally, the filtrate was concentrated using methods such as rotary evaporation or vacuum distillation to obtain a concentrated extract for further analysis like alpha-amylase inhibition assay and alpha-glucosidase inhibition assay. RESULTS: The ethanolic extracts derived from dried onion peel demonstrate inhibition of alpha-glucosidase, leading to reduced blood glucose levels. Additionally, this inhibition prompts an increase in insulin production. CONCLUSION: The study underscores that the efficacy of the ethanolic extract of dried onion peel increases with concentration. It highlights the presence of beneficial compounds like total phenolics, flavonoids, quercetin, and its derivatives in onion peel, known for their therapeutic roles in cardiovascular health, weight management, diabetes control, cancer prevention, and antimicrobial activity. These findings affirm the hypoglycemic and anti-diabetic properties of Allium cepa's ethanolic leaf extract.

Assessment of Nanoscale Configuration of Dentin in Non-carious Cervical Lesions.

Introduction Non-carious cervical lesions (NCCLs) are structural deformities or tissue loss in the cervical region of teeth, which are closer to the gum line that do not result from caries (tooth decay). On the tooth enamel at the gingival margin, these lesions are frequently seen as V- or U-shaped notches. The causes of NCCLs are multifaceted and involve intricate relationships between chemical, mechanical, and biological elements. The slow loss of enamel and dentin in the cervical area occurs due to mechanical causes like abrasive toothbrushing and occlusal tension, as well as chemical variables such as erosive acidic food components. As NCCLs advance, dental discomfort, aesthetic issues, and impaired tooth function may result. The aim of this study was to assess the nanoscale configuration of dentin in non-carious cervical lesions using scanning electron microscopy (SEM) and energy-dispersive spectroscopy (EDS). Methodology Sterilized teeth samples were selected from the extracted tooth repository of Saveetha Dental College and Hospitals, Chennai. Tooth samples were thinly sliced using a diamond bur. The sliced teeth were examined using a scanning electron microscope. Ionic configuration was assessed using EDS and elemental analysis. Results The findings showed the nanoscale morphology and elemental configurations present. Elemental mapping showed specific elemental localization in the affected area. Conclusion NCCLs can be attributed as a predominant factor leading to gingival recession causing dentin exposure and hypersensitivity. NCCLs can also threaten the integrity of dentin; therefore, early diagnosis of non-carious cervical lesions is crucial to ensure proper treatment plan and therapeutic regimens.

Camellia sinensis Assisted Synthesis of Copper Oxide Nanoparticles (CuONPs) and Assessment of Its Antioxidant Activity and Zebrafish Embryonic Toxicology Evaluation.

BACKGROUND: - Camellia sinensis, or oolong tea, is a partially fermented version of tea used in Asian countries. The remarkable reduction activity of the tea extract can potentially be used for synthesizing nanoparticles. Recently, Camellia sinensis has gained popularity for the formulation of some metal nanoparticles. Aim To formulate green synthesis of copper oxide nanoparticles (CuONPs) mediated by Camellia sinensis (oolong tea) and assess its cytotoxicity and antioxidant properties. Materials & Methods Oolong tea extract is prepared and added to CuSO4 solution to synthesize CuO nanoparticles (CuONPs). The centrifugation pellet of CuONPs is collected and subjected to DPPH (2,2 - diphenyl -1- picrylhydrazyl hydrate) and H2O2 assays. The cytotoxicity screening is performed using zebrafish embryos. Results The reducing activity of oolong tea successfully synthesizes the copper nanoparticles. High values are obtained in DPPH (63% inhibition at 10µL concentration, 73% inhibition at 20µL, 80% at 30µL, 85% at 40µL and 90% at 50µL concentrations) and H2O2 (50% inhibition at 10µL concentration, 65% at 20µL, 68% at 30µL, 75% at 40µL and 80% at 50µL concentrations) assays. There are no morphological deformities in the zebrafish and no loss of cell viability or delayed hatching at low concentrations (below 4-8 µL), as shown by the viable embryos with no morphological deformities. Conclusion The study has evidenced high antioxidant activity and minimal cytotoxicity of CuO nanoparticles produced using Camellia sinensis, thus proving it to be a good biomaterial for a wide range of biological applications.

Effect of COVID-19 on Dental Education: A Review.

Corona Virus Disease (COVID-19) has become a pandemic and a real threat for those working in healthcare. It has affected dental professionals in education, research, and practice. This article intended to review the impact and brunt of COVID-19 outbreaks on dental education and research country wise and how it affected the three learning domains - cognitive domain, psychomotor domain and affective domain. A review was designed to identify the impact of the COVID-19 epidemic on dental education. A literature search was conducted using PubMed, Scopus and Google scholar databases. Studies in which teaching methods by virtual means were described and how it affected dental education during the pandemic all over the world were included. The search terms selected to search for literature were dental education, COVID-19, dental schools and dentistry. COVID-19 has caused a significant change in overall dental education in all the countries. It has affected dental education both negatively and positively. The pandemic caused disruption in learning leading to psychological distress. Even though many students preferred web-based learning, majority of them considered learning through online mode as a challenge. COVID-19 pandemic has enforced all the dental schools to modify their traditional way of teaching to an alternate mode of teaching to adapt to this current situation of the pandemic all over the world. It caused an everlasting impact on dental education, research along with practice. The dental institutes must be well prepared to face this pandemic by investing in educational software so that it leads to the evolution and advancement in the fields of virtual mode of teaching, in research and in preclinical training.

Progressive muscle relaxation technique on anxiety and depression among persons affected by leprosy.

Anxiety and depression have been found to be increasing among people with leprosy and it may lead to decreased social participation. The progressive muscle relaxation technique (PMRT) is widely used today in choice of treatment for reducing the anxiety and depression. This study aimed to assess the effectiveness of PMRT in reducing anxiety and depression among the hospitalized leprosy affected person in a tertiary care centre. This study is a case series of 50 leprosy affected people aged between 18-60 years who were admitted for leprosy complications in tertiary leprosy referral hospital. The Anxiety-Depression scale was developed and validated by the investigators and administered before intervention of PMRT and after 2 weeks. The follow-up assessment was done at 6 weeks after the initial intervention. The finding shows that a statistically significant difference was observed on anxiety domain before and after application of PMRT. The anxiety means score showed steady decline from 6.76 at pretest to 3.0 (t=25.068, P≤0.001) at post test and 1.12 (t=22.679, P≤0.001) at follow-up. In depression domain, a statistically significant difference was seen in before and after application of PMRT. The depression means score showed steady decline from 6.92 at pre test to 3.28 (t=16.082, P≤0.001) at post test and to 1.16 (t=18.918, P≤0.001) at follow-up. This study proved that the PMRT as a valid treatment option for hospitalized person with leprosy in minimizing the anxiety and depression related symptoms and to benefit the psychosocial wellbeing of leprosy affected patients.