Bioglass/ceria nanoparticle hybrids for the treatment of seroma: a comparative long-term study in rats.

Background: Seroma formation is a common postoperative complication. Fibrin-based glues are typically employed in an attempt to seal the cavity. Recently, the first nanoparticle (NP)-based treatment approaches have emerged. Nanoparticle dispersions can be used as tissue glues, capitalizing on a phenomenon known as 'nanobridging'. In this process, macromolecules such as proteins physically adsorb onto the NP surface, leading to macroscopic adhesion. Although significant early seroma reduction has been shown, little is known about long-term efficacy of NPs. The aim of this study was to assess the long-term effects of NPs in reducing seroma formation, and to understand their underlying mechanism. Methods: Seroma was surgically induced bilaterally in 20 Lewis rats. On postoperative day (POD) 7, seromas were aspirated on both sides. In 10 rats, one side was treated with NPs, while the contralateral side received only NP carrier solution. In the other 10 rats, one side was treated with fibrin glue, while the other was left untreated. Seroma fluid, blood and tissue samples were obtained at defined time points. Biochemical, histopathological and immunohistochemical assessments were made. Results: NP-treated sides showed no macroscopically visible seroma formation after application on POD 7, in stark contrast to the fibrin-treated sides, where 60% of the rats had seromas on POD 14, and 50% on POD 21. At the endpoint (POD 42), sides treated with nanoparticles (NPs) exhibited significant macroscopic differences compared to other groups, including the absence of a cavity, and increased fibrous adhesions. Histologically, there were more macrophage groupings and collagen type 1 (COL1) deposits in the superficial capsule on NP-treated sides. Conclusion: NPs not only significantly reduced early manifestations of seroma and demonstrated an anti-inflammatory response, but they also led to increased adhesion formation over the long term, suggesting a decreased risk of seroma recurrence. These findings highlight both the adhesive properties of NPs and their potential for clinical therapy.

Free Tissue Transfer in Head and Neck Reconstruction: A Multidisciplinary 15-Year Experience.

BACKGROUND: Free tissue transfer is considered the gold standard in reconstruction of extensive defects in head and neck surgery. The aim of this 15-year retrospective study is to analyze the outcomes of free tissue transfers in the head and neck area in a tertiary referral university hospital. MATERIALS AND METHODS: A retrospective, single-center study of all patients undergoing free tissue transfers for head and neck reconstruction between 2006 and 2020 was performed. Patient demographics, comorbidities, flap characteristics, outcomes and complications were assessed. RESULTS: A total of 353 free flaps were performed. The most common defect etiology was synchronous oncologic resection (74.2%). The majority of patients had at least one comorbidity (70.3%), with smoking recorded in 46.2% of the cases and alcohol consumption in 31.7%. The anterolateral thigh flap was the most commonly used flap (37.7%), followed by the osteoseptocutaneous fibula flap (26.9%). Our overall flap success rate was 97.7%, while the overall complication rate was 45.9%. CONCLUSIONS: Free tissue transfer in head and neck reconstruction is reliable. However, complication rates remain high due to the complexity of such cases and frequent presence of comorbidities. Nonetheless, when effectively managed within a multidisciplinary team, complications rarely jeopardize the overall reconstruction outcome.

Management of Postoperative Seroma: Recommendations Based on a 12-Year Retrospective Study.

Introduction: Seroma formation is a serious postoperative complication. Since the management algorithms available in the literature are scarce, we aimed to analyze our experience with postoperative seroma in order to identify indicators for revisional surgery and propose recommendations for management. Methods: This retrospective study included all patients with postoperative seroma treated in a tertiary university hospital from 2008 to 2020. Patients' demographics, medical history, and seroma treatment details were recorded and analyzed. Results: Overall, 156 patients were included: 41% were initially treated through needle aspiration, with 61% eventually undergoing surgical treatment for postoperative seroma. Comorbidities, such as heart failure and coronary heart disease, were significantly associated with an increased need for revisional surgery (p < 0.05). Both a duration of >40 days of repeated needle aspirations and drain re-insertions were significantly correlated with an increased risk for revisional surgery (p < 0.05). Conclusion: Patients requiring seroma aspiration should be counseled on surgical treatment sooner rather than later, as prolonged aspiration time (over 40 days) greatly increases the risk of surgical revision. Moreover, the reinsertion of a drain should only be used as a temporizing measure, at most, and patients requiring a drain to control the size of the seroma should promptly be scheduled for a surgical revision.

Distraction Osteogenesis Technique for the Management of a Gustillo Type I Tibial Shaft Fracture Initially Managed with an Intramedullary Nail Device.

Fractures of the tibia shaft are the most common long bone fractures, especially in young male adults. Due to specific anatomical features, these fractures are more common than any other long-bone fracture. This is one of the main reasons they are associated with twice the incidences of deep infection compared with any other bone and can be combined with the presence of segmental bone defect. Many reconstruction techniques have been used to manage such cases, including Ilizarov technique, Masquelet technique, vascularized fibula transfer, and the monolateral external fixator. We present a case of a 21-year-old male patient who was admitted to our hospital for a Gustillo type I tibial shaft fracture which was initially treated by an intramedullary nail device. Two months postsurgery the patient presented with clinical signs of infection in the area of the fracture site, confirmed by an osteolytic lesion revealed radiographically. A surgical operation was performed that included a tibial osteotomy proximal to the defected bone, bone excision, and application of a LRS external fixation device. Antibiotic therapy was administrated based grown cultures, and regular follow-up X-ray revaluation was performed. After six months, the bone was radiologically united, the frame was removed, and the leg was protected in a walking boot while the patient used only partial weight bearing. The treatment of segmental bone defects associated with infection and soft tissue loss presents a great challenge, and the choice of the appropriate method requires further study.

Fusobacterium Nucleatum Causing Necrotizing Fasciitis of the Neck, Thorax, and Upper Limb: A Lemierre's-Like Case Presentation.

Necrotizing fasciitis (NF) is a rare "flesh-eating" entity with a high mortality rate due to late diagnosis. More specifically, this disease is deemed to be a subset of the aggressive skin and soft tissue infections (SSTIs) resulting in necrosis of the muscle fascia and subcutaneous tissues. It is usually caused by Gram-positive cocci specifically strains of Staphylococcus aureus and Streptococci or the combination of Gram-negative and anaerobic bacteria. If septic thrombophlebitis complicates a parapharyngeal abscess the clinical condition is referred to as Lemierre's syndrome (LS), which is also a rare entity and can result in necrotizing fasciitis of the neck and is usually caused by Fusobacterium necrophorum or Fusobacterium nucleatum. In our study, a rare case of a 61-year-old male patient who presented with progressed necrotizing fasciitis of his neck, chest, as well as his upper arm and a history of a bacterial throat infection in our emergency department is discussed. Despite treatment involving iv antibiotics and urgent radical surgical debridement, the patient unfortunately succumbed to his disease due to sepsis and secondary multiorgan failure.

Primary Extranodal Skeletal-Muscle Non-Hodgkin B Cell Lymphoma: Clinical Presentation and Diagnostic Approach.

Primary extranodal non-Hodgkin lymphomas that involve skeletal muscles (PSML) are infrequent with non- specific features or symptoms. Therefore, their diagnosis can be immensely convoluted since they mimic other soft tissue tumors and diseases (34). In this study, the case of a 61-year-old male patient, who presented with a history of a 6-week left thigh oedema and concomitant pain in our Emergency Department, is discussed. The patient was initially reviewed in another institution; the results of imaging studies (ultrasound scan) were consistent with deep vein thrombosis (DVT).Despite treatment, the patient's pain and swelling was exacerbating, which forced him to visit our hospital. Magnetic resonance imaging (MRI) revealed a diffused mass in his right thigh, while fine needle aspiration cytology (FNAC) yielded a diagnosis of B-cell lymphoid hyperplasia. The patient was then referred to a tertiary cancer treatment center for further management.

Bilateral Simultaneous Proximal Tibial Insufficiency Fractures in a Patient Suffering From Psoriatic Arthritis.

Bilateral synchronous proximal tibia insufficiency fractures are rarely reported. We present a case of simultaneous proximal tibia bilateral insufficiency fractures in a 51-year-old female patient with underlying psoriatic arthritis, who was on chronic steroid medication. She reported sudden onset of bilateral knee pain after intense workout one week ago. Initial clinical and X-ray evaluation did not reveal significant pathology. Four weeks later, due to persistent pain in the absence of significant radiographic findings during follow-up, the patient was referred for MRI, which revealed fractures of both proximal tibias. A "mixed" treatment protocol was applied. In particular, this protocol included combination of rest and intermittent removable knee ranger braces immobilization with weight-bearing when applied. The patient went on to make a full recovery. Chronic inflammatory disorders accompanied by suspicious clinical manifestations should be thoroughly inspected. Diagnostic and treatment protocols should be further discussed and implemented.

Lower Limb Pain Attributed to Bone Marrow Edema Syndrome: A Commonly Ignored Pathology.

Bone marrow edema syndrome (BMES) is a highly uncommon, self-limited syndrome of unclear etiology. The syndrome most commonly affects middle-aged men. Magnetic resonance imaging is essential for the diagnosis because of the characteristic pattern of bone marrow edema. The diagnosis of BMES is a challenge for clinicians. Other causes of lower extremity pain, with poor prognosis, must be excluded. We present three cases of BMES. All three patients initially complained of mild lower extremity pain, which progressively deteriorated and led to a severe limitation of their daily activities. They were all treated conservatively by weight-bearing restriction and symptoms resulted within a few months. The aim of the present study is to outline this rare, benign pathology.

Spontaneous Knee Hemarthrosis Due to Hypofibrinogenemia Following Tigecycline Treatment for Periprosthetic Joint Infection.

Tigecycline, a recently approved antibiotic, has a broad spectrum of antimicrobial activity. Its unique structure and properties make tigecycline a valuable option for the treatment of infections caused by many multidrug-resistant organisms. We present a case of an 82-year-old patient who developed a significant decrease of fibrinogen levels after the addition of tigecycline to his antibiotic regimen. The patient was treated for a periprosthetic knee joint infection caused by a multidrug-resistant extended-spectrum beta-lactamase-producing Escherichia coli. The reduction of fibrinogen levels, in this case, prompted severe spontaneous hemarthrosis. Tigecycline treatment was discontinued and coagulation disorders were normalized within the next few days. After several days, the joint had to be surgically debrided. Hypofibrinogenemia is a very scarcely reported side effect of tigecycline that can cause spontaneous hemarthrosis.

The Use of Vacuum Closure-assisted Device in the Management of Compound Lower Limb Fractures with Massive Soft Tissue Damage.

The simultaneous exposure of tissue and bone poses specific management challenges. Patients with extended soft tissue damage and high-grade compound fractures present a demanding clinical challenge, requiring a complex approach and multiple orthopaedic, plastic, and vascular-reconstructive procedures. Management involves combinations of wound debridement and closure by secondary intention, use of vacuum-assisted closure (VAC) devices, and various reconstructive plastic surgery methods. We present three consecutive complicated cases, involving compound fractures of the lower limb with massive soft tissue damage (Gustilo-Anderson type IIIB) that were managed with debridement, application of external fixation and VAC device. The mean wound size was 24 cm in length and 12 cm in width. The aim of treatment was to cover the bone with soft tissue and achieve healing of the fracture without persistent infection. Wound healing was achieved in all three cases within 30-42 days (mean 34). In one case, the skin graft was applied on day 33. Utilizing this method as part of a multi-directional approach, the VAC system helps the patient recover faster. Moreover, it acts as a feasible and valuable method to treat compound fractures with massive soft-tissue defects. VAC can replace microsurgical soft-tissue transfer, reduce the risk of infection and allow salvaging the limb.

Nontraumatic Osteolysis of the Distal Clavicle: Diagnostic Approach and Management.

Osteolysis of the distal clavicle is a rare radiological manifestation. It can be of traumatic or nontraumatic origin and is usually seen in weight-lifting trainers as a result of stress-induced osteolysis of the distal clavicle, a phenomenon known as stress failure syndrome. We present a rare case of nontraumatic osteolysis of the distal clavicle in a 65-year-old, male, non-weight lifter who was referred to our outpatient clinic. Osteolysis was revealed on plain radiographs and was confirmed with magnetic resonance imaging. Diagnostic approach, differential diagnosis, and management of this rare manifestation are discussed.

SEPSIS SECONDARY TO DELAYED PRESENTATION OF NECROTISING FASCIITIS OF THE THIGH-PRESENTATION OF TWO CASES WITH A POOR OUTCOME.

Necrotizing fasciitis is an extremely virulent form of infectious fasciitis. It affects skin, subcutaneous fat, and superficial and deep muscular fascia by rapidly progressive necrosis. We present two cases, a 54-year-old female patient and a 46-year-old male patient, who presented to the emergency department of our hospital 30 days and 14 days after the onset of symptoms, respectively, with progressively deteriorating pain and swelling of the thigh, accompanied by fever, nausea, sweating, shortness of breath, and sepsis. Clinical, laboratory, and imaging findings suggested thigh fasciitis, which was secondary to osteomyelitis of the femoral head in one case. Despite the urgent surgical exploration and debridement, multiple courses of various schemes of intravenous antibiotics administered, the numerous surgical debridements and interventions performed, and the prolonged hospitalization in the intensive care unit, both patients died due to multiple-organ failure and disseminated intravascular coagulation. Necrotizing fasciitis is of surgical urgency with a high mortality rate, and early surgical intervention is of vital importance. In both of these cases, delayed presentation to the hospital was of great significance to the final outcome.

Scapulothoracic Dissociation and Clavicle Fracture with Associated Brachial Plexus Palsy.

Scapulothoracic dissociation is the traumatic separation of the scapula from the thoracic wall. It is the outcome of rare, high-energy trauma to the shoulder girdle and may include vascular and neurologic damage of the upper extremity. Of the few cases presented in the literature, the majority refer to polytrauma patients. We present a rare variant of scapulothoracic dissociation combined with comminuted fractures of both the scapula and the clavicle associated with brachial plexus palsy. There were also vertebral fractures of the spinous processes of C6, T4, T5, and T6, of the transverse processes of T1 and T2, and of the anterior and middle column of T12. An open reduction and internal fixation of the clavicle was performed, and the patient was hospitalized in the intensive care unit and on the ward. The patient had a good recovery, involving extensive physiotherapy during hospitalization and after discharge to a rehabilitation center. Scapulothoracic dissociation can have potentially disastrous implications. The diagnosis is sometimes challenging because it usually involves polytrauma patients who have multiple life-threatening injuries. The diagnosis and management of this injury is of significant importance, and immediate surgical intervention should be performed when required.

Metastatic Melanoma to the Urinary Bladder of Ocular Origin Accompanied with Primary Cutaneous Melanoma: Diagnostic Challenge-A Report of a Case.

Metastases of melanoma to the urinary bladder are infrequent. Even rarer are metastases to the urinary bladder from uveal melanoma, with only 3 cases published in the literature so far. Herein we present a case of a 77-year-old male patient who presented with metastatic melanoma to the urinary bladder. The patient's history included the diagnoses of uveal melanoma treated with radiation 25 years ago, as well as that of cutaneous melanoma diagnosed 7 years ago. The molecular study of the urinary bladder tumor specimen identified mutation of the GNAQ gene, which has been suggested to be an early molecular event in the pathogenetic course of over 80% of uveal melanomas. Therefore, the diagnosis of uveal melanoma metastatic to the urinary bladder was made.

Autonomous Ovarian Cysts in Prepubertal Girls. How Aggressive Should We Be? A Review of the Literature.

STUDY OBJECTIVE: Precocious pseudopuberty in girls occurs as a result of peripheral estrogen production, most commonly through an autonomous ovarian cyst or in patients with McCune Albright syndrome. Current management is either conservative or surgical. The aim of this study was to identify available literature on presentation, course and treatment of such cases. DESIGN: We performed a medline literature search using the key words 'ovarian autonomous cyst' and 'pseudopuberty'. We included articles published in English, from 1980 to date relating to females aged 2 to 8 years. We excluded papers on other endocrine conditions resulting in premature puberty. OUTCOME MEASURES: Type of management, time of resolution, recurrence, progression to Central Precocious Puberty and McCune Albright Syndrome. RESULTS: We identified 9 articles referring to 26 cases of autonomous ovarian cysts. All patients had suppressed LHRH testing and had simple ovarian cysts producing estradiol. Median age of patients was 5 years (range 2.2-8) and size of cyst was 41.5mm (9-60). Ten patients underwent cystectomy or oophorectomy, three were given cyproterone acetate and 13 were managed conservatively. Nine of 13 patients that were managed conservatively and two of those that had a cystectomy had a recurrence. There were three cases that progressed to central precocious puberty (CPP) and one case that was then identified to have McCune Albright (MCA) syndrome. One further case with multiple recurrences was given long term treatment with letrozole. CONCLUSION: We conclude that although a conservative approach should always be proposed in the first instance, one should be aware of the risk of recurrence and progression to CPP or MCA. Antiestrogen treatment appears promising; however data comparing it with surgical options and particularly long term consequences with regards to future reproductive outcomes are not available.

Imaging of the malignant peripheral nerve sheath tumour with emphasis οn ultrasonography: correlation with MRI.

Malignant peripheral nerve sheath tumours affect 0.001 % of the population. The epithelioid subtype accounts for only 5 % of them. We herein present a patient with a palpable mass on the left calf, which was investigated by ultrasonography, ultrasonographically guided fine-needle biopsy and MRI. Ultrasonography estimated the size of the tumour and evaluated its echotexture. Doppler technique detected characteristic corkscrew-type blood vessel entering in the upper pole of the mass. T1-weighted, fat-suppression and gadolinium-enhanced MRI sequences confirmed ultrasonographic findings. In conclusion, although final diagnosis was established by biopsy, ultrasonography and MRI proved complementary in the characterization of the lesion and planning of surgery.

Platelet-rich plasma versus autologous whole blood for the treatment of chronic lateral elbow epicondylitis: a randomized controlled clinical trial.

BACKGROUND: Chronic lateral elbow epicondylitis is a tendinosis with angiofibrolastic degeneration of the wrist extensors' origin. Healing of this lesion is reported with the use of autologous blood as well as with platelet-rich plasma (PRP). PURPOSE: A comparative study of these 2 treatments was conducted in an effort to investigate the possible advantages of PRP. STUDY DESIGN: Randomized controlled trial; Level of evidence, 1. METHODS: Twenty-eight patients were divided equally into 2 groups, after blocked randomization. Group A was treated with a single injection of 3 mL of autologous blood and group B with 3 mL of PRP under ultrasound guidance. A standardized program of eccentric muscle strengthening was followed by all patients in both groups. Evaluation using a pain visual analog scale (VAS) and Liverpool elbow score was performed at 6 weeks, 3 months, and 6 months. RESULTS: The VAS score improvement was larger in group B at every follow-up interval but the difference was statistically significant only at 6 weeks, when mean improvement was 3.8 points (95% confidence interval [CI], 3.1-4.5) in group B (61.47% improvement) and 2.5 points (95% CI, 1.9-3.1) in group A (41.6% improvement) (P < .05). No statistically significant difference was noted between groups regarding Liverpool elbow score. CONCLUSION: Regarding pain reduction, PRP treatment seems to be an effective treatment for chronic lateral elbow epicondylitis and superior to autologous blood in the short term. Defining details of indications, best PRP concentration, number and time of injections, as well as rehabilitation protocol might increase the method's effectiveness. Additionally, the possibility of cost reduction of the method might justify the use of PRP over autologous whole blood for chronic or refractory tennis elbow.

Pyomyositis and septic hip arthritis due to bacteroides fragilis. A case report.

Bacteroides fragilis is a rare cause of pyomyositis or septic arthritis. We present the case of a 74-year-old otherwise healthy male who presented with fever and right hip pain ten days after a course of intramuscular injections. Magnetic resonance imaging (MRI) showed septic arthritis of the right hip joint and pyomyositis of the right gluteus minimus muscle. Blood cultures and pus aspirated from the muscle grew Bacteroides fragilis. The patient was treated successfully with surgical debridement and metronidazole, administered for six weeks intravenously and five weeks orally. Simultaneous pyomyositis and hip septic arthritis due to Bacteroides fragilis is rare, but should be considered possible after intramuscular injections.

Irreducible Lisfranc dislocation due to the interposition of the tibialis anterior tendon: case report and literature review.

We report a case of a lateral type A (according to Myerson) Lisfranc dislocation irreducible by closed manipulation. Upon surgical exploration the lateral slip of the tibialis anterior tendon was found trapped between the medial and the middle cuneiform. Once the tendon was reduced, the dislocated first ray spontaneously reverted to its anatomical position and the joint was fixed with Kirschner wires. Seven similar cases have been reported in the literature. After analysis of all the reported cases we were able to describe a pattern of injury that may predict tibialis anterior interposition: (a) lateral type A according to Myerson dislocation; (b) after closed manipulation the 1st ray remains dislocated while the 5th metatarsal reduces; (c) there is a gap between the medial and the middle cuneiform or a fracture at the base of the 2nd metatarsal.