Examining the immunotoxicity of oil sands process affected waters using a human macrophage cell line.

Oil sands process affected water (OSPW) is produced during the surface mining of the oil sands bitumen deposits in Northern Alberta. OSPW contains variable quantities of organic and inorganic components causing toxic effects on living organisms. Advanced Oxidation Processes (AOPs) are widely used to degrade toxic organic components from OSPW including naphthenic acids (NAs). However, there is no established biological procedure to assess the effectiveness of the remediation processes. Our previous study showed that human macrophage cells (THP-1) can be used as a bioindicator system to evaluate the effectiveness of OSPW treatments through examining the proinflammatory gene transcription levels. In the present study, we investigated the immunotoxicological changes in THP-1 cells following exposure to untreated and AOP-treated OSPW. Specifically, using proinflammatory cytokine protein secretion assays we showed that AOP treatment significantly abrogates the ability of OSPW to induce the secretion of IL-1ß, IL-6, IL-8, TNF-α, IL-1Ra and MCP-1. By measuring transcriptional activity as well as surface protein expression levels, we also showed that two select immune cell surface markers, CD40 and CD54, were significantly elevated following OSPW exposure. However, AOP treatments abolished the immunostimulatory properties of OSPW to enhance the surface expression of these immune proteins. Finally, a transcriptome-based approach was used to examine the proinflammatory effects of OSPW as well as the abrogation of immunotoxicity following AOP treatments. Overall, this research shows how a human macrophage cell-based biomonitoring system serves as an effective in vitro tool to study the immunotoxicity of OSPW samples before and after targeted remediation strategies.

A Study to Assess and Evaluate the Gingival Response During and after the Fixed Orthodontics Treatment Experienced by Adult Patients.

Objective: The purpose of this research was to examine patients' periodontal health before, during, and after treatment using a fixed orthodontic device. Methods: Forty normal teenagers with full sets of permanent teeth and a clinical need for fixed orthodontics participated. Individuals were chosen from the Class I population who would benefit from utilizing traditional orthodontic braces and techniques that did not need extractions. Respondents were sent to the periodontist for required checkups after the placement of a fixed orthodontic device at 3-, 6-, 12-, and 24-month intervals, respectively, till the conclusion of orthodontic treatment. Results: After having a fixed orthodontic device put in place, patients had a rise in the average values of the Plaque Index and Sulcus Bleeding Index at each checkup. Keeping an eye on the progression three months, six months, a year, and two years after the initiation of orthodontic treatment reveals a statistically significant difference in the presence of gingival hyperplasia. Conclusion: Patients' periodontal health before, during, and after fixed orthodontic treatment shows the need for close collaboration between the orthodontist and the periodontist.

A Study to Assess Knowledge, Attitude, and Perception of Dental Practitioners on Minimally Invasive Dentistry Concepts.

Background: Minimally invasive dentistry (MID) includes caries detection using caries risk assessment (CRA), early localization of nascent carious sores including necessary and voluntary avoidance, and remineralization of demineralized veneer and dentin when diagnosed early for these reasons. Although the dentistry curriculum prioritizes the benefits of MID in tooth protection, this research is seldom included in classroom instruction. Aim: The purpose of this research was to gauge the level of understanding that dental professionals have about MID ideas. Methods: An online questionnaire measuring MID knowledge and application was used to compile the data. The Chi-square tests were run for statistical analysis in the Statistical Package for the Social Sciences (SPSS). Results: Just 42.2% of the dentists who replied had MID training during their undergraduate or internship years, and the majority of that training came via lectures (45.2%). The majority (almost 81%) of respondents believed that CRA should be performed on all patients. Differences in knowledge and experience with atraumatic restorative therapy and the sandwich approach for treating caries in permanent teeth and high caries risk children were shown to be statistically significant (P 0.05). Conclusion: Dentists know about the benefits of MID, but their patients are not reaping those benefits since so many dentists still use the old methods of treating complete caries.

A Study to Assess Etiology and Prevalence of Signs and Symptoms of Temporomandibular Disorder.

Aim: The purpose of this epidemiological research was to determine how common temporomandibular disorder (TMD) symptoms are. Materials and Methods: Among the outpatient population, a sample of 100 people was chosen at random. A patient survey was presented to each person, and points were allotted based on their responses. Participants were categorized as having no symptoms of TMD, mild TMD, moderate TMD, or severe TMD based on their total score. Results: One hundred adults aged 18 and above were selected from the general public to take part in the research. Temporomandibular disorder was present in no more than 30% of those studied, in the mild range in 50%, in the moderate range in 15%, and in the severe range in 5%. 0.602 was determined to be the dependability of Fonseca's questionnaire. Conclusion: The Fonseca questionnaire is a useful instrument for gauging the extent to which TMD symptoms are present in a given population. The screening questionnaire may be received in a short amount of time and for little money, making it a good option for public health services.

Tumour necrosis factor-α -308G/A polymorphism is associated with insulin secretory defects in Bangladeshi prediabetic/diabetic subjects.

Objectives: Tumour necrosis factor (TNF)-α, an adipocytokine, is closely linked to impaired glucose tolerance (IGT) and insulin resistance (IR) in type 2 diabetes (T2D) subjects. The relationship between the polymorphisms in the TNF-α gene and IR in Bangladeshi prediabetes and T2D subjects has not yet been fully identified. This study aims to reveal the association between TNF-α gene polymorphism and IR in hyperglycaemic patients of Bangladeshi origin. Methods: In our study, 106 IGT, 100 T2D, and 109 healthy subjects of Bangladeshi origin were recruited to identify the impact of TNF-α gene polymorphism at position -308 with a G>A transition using PCR and subsequent restriction fragment length polymorphism (RFLP). Results: The -308G>A TNF-α genotype frequency distribution within the control, IGT, and T2D groups showed a significant association (χ2 = 21.077; P = 0.001), although allele frequency distribution within the groups showed a statistically non-significant difference (χ2 = 1.696; P = 0.091). ß-cell functional deficiency (HOMA-B%) was observed to be significantly (P = 0.034) lower in subjects with a variant genotype. In addition, our results indicate that the study subjects' body mass index (BMI) and residence status were positively correlated (P ≤ 0.05) with -308G>A TNF-α gene polymorphism. Conclusions: Therefore, it can be concluded that -308G>A TNF-α gene polymorphism may have a causative relationship with lower insulin secretory capacity and higher BMI in Bangladeshi IGT and T2D populations, while the urban population's lifestyle might be associated with this polymorphism.

Solar-activated zinc oxide photocatalytic treatment of real oil sands process water: Effect of treatment parameters on naphthenic acids, polyaromatic hydrocarbons and acute toxicity removal.

Oil sands process water (OSPW) is an industrial process effluent that contains organic compounds such as naphthenic acids (NAs) and polyaromatic hydrocarbons (PAHs), as well as large quantities of inorganic compounds in its mixture. OSPW requires effective treatment for successful reclamation and water reuse. This study investigated the impact of solar-activated zinc oxide (ZnO) photocatalysis on the degradation and removal of NAs and PAHs in OSPW, as well as the elimination of its acute toxicity. With catalyst particles suspended in the effluent (at 1 g/L) under simulated solar radiation of steady irradiance of ~278 W/m2, more than 99% removal of NAs was achieved after 4 h of treatment, while nearly all PAHs were simultaneously oxidized within the same reaction time. The photocatalytic treatment appeared to selectively convert classical NAs faster than oxidized NAs. Additionally, NAs with higher double-bond equivalents (DBEs) and higher carbon numbers seemed more susceptible to photocatalytic destruction than others. An overall pseudo first-order rate constant of 1.14 × 10-2 min-1, and a fluence-based rate constant of 6.81 × 10-1 m2/MJ were recorded in apparently hydroxyl radicals (OH) and superoxide (O2-) radicals mediated NAs degradation mechanisms. Assessment of the toxicity levels in raw and treated OSPW samples by using Microtox® bioassay indicated that the photocatalytic treatment resulted in ~50% reduction in acute toxicity. Furthermore, we showed that by monitoring the expression levels of key proinflammatory genes using qPCR that treated OSPW significantly reduced the ability of raw OSPW to activate the inflammatory response of immune cells. This indicates that at acute sub-lethal exposure doses, photocatalytic treatment also reduces immunotoxicity. Overall, our results suggest that the ZnO-based photocatalytic degradation of these NAs and PAHs in OSPW could be a significant treatment process aimed at detoxifying OSPW.

Compositional features and pattern of codon usage for mitochondrial CO genes among reptiles.

The phenomenon of non-random occurrence of synonymous nucleotide triplets (codons) in the coding sequences of genes is the codon usage bias (CUB). In this study, we used bioinformatic tool kit to analyze the compositional pattern and CUB of mitogenes namely COI, COII and COIII across different orders of reptiles. Estimation of overall base composition in the protein-coding sequences of COI, COII and COIII genes of the reptilian orders revealed an uneven usage of nucleotides. The overall count of A nucleotide was found to be the highest while the overall count of G nucleotide was the least. The CO genes across the three reptilian orders were prominently AT biased. Comparison of the GC proportion at each codon position displayed that GC1 percentage ranked the highest in all the three CO genes of the reptilian orders. SCUO values indicated weaker CUB, while considerable variation of SCUO values existed in the three CO genes across the studied reptiles. Relative synonymous codon usage (RSCU) values indicated that mostly the A ending codons were preferred. Based on the parameters namely neutrality plot, mutational responsive index and translational selection, we could conclude that natural selection was the major evolutionary force in COI, COII and COIII genes in the studied reptilian orders. However, correspondence analysis, parity plot and correlation studies indicated the existence of mutation pressure as well on the CO genes.

Identification of novel microRNAs in Rous sarcoma Virus (RSV) and their target sites in tumor suppressor genes of chicken.

A small non-coding, evolutionarily conserved regulatory RNA molecule known as microRNA (miRNA) regulates various cellular activities and pathways. MicroRNAs remain evolutionarily conserved in different species of same taxa. They are present in all organisms including viruses. Viral miRNAs are small, less conserved and less stable and have higher negative minimal folding free energy than miRNAs of different organisms. The size of viral precursor miRNA is approximately 60-119 nucleotides in length. The structure of the mature miRNA sequences is predicted by using higher negative MFE (ΔG) value. Rous sarcoma Virus (RSV), named after its inventor Peyton Rous, has been known for causing tumors in the chicken for which it is known as an oncogenic retrovirus. Using specific criteria we have predicted 5 potential miRNAs in RSV which targeted 8 tumor suppressor genes in Gallus gallus. This study aims to predict the potential miRNAs, secondary structures and their targets for better understanding of the regulatory network of Rous sarcoma virus miRNA in forming sarcoma.

A Crosstalk on Codon Usage in Genes Associated with Leukemia.

Leukemia is the outcome of aggregation of damaged white blood cells. Several genes were reported to be associated with the pathogenesis of leukemia. These genes were computationally analyzed to decipher their codon usage bias (CUB) and to identify the prime factors influencing the codon usage profile as no work was reported yet. The mean values of synonymous codon usage order (SCUO) parameter indicated low CUB of the genes. Significant positive association of SCUO with overall GC and positional GCs might signal the presence of mutational pressure. However, neutrality plot suggested the dominant role of natural selection across the genes. Along with natural selection, the role of mutation pressure was also prominent and that might be responsible for lower CUB (SCUO = 0.19) of genes. Low translational speed might permit accuracy in the process. A strong inverse relationship of translational rate was observed with CUB of genes and folding energy.

Synonymous codon usage and context analysis of genes associated with pancreatic cancer.

Pancreatic cancer is a fatal disorder which originates in pancreas. Its mortality rate is increasing with time. Some studies also reported that pancreatic cancer would be ranked 2nd by the year 2030. Codon usage bias (CUB) arises when synonymous codons for each amino acid are not used randomly in the coding sequences of genes. We used bioinformatic methods to analyze the compositional properties, codon context and codon usage trend of the genes associated with pancreatic cancer as no work was reported yet. From the base composition analysis, the pancreatic cancer genes were found to be GC-rich and at the 3rd codon position the G/C ending codons were more preferred to A/T ending ones. The CUB was low in genes associated with pancreatic cancer. Correspondence analysis proposed that other than base constraints, CUB might also be affected by some other factors such as natural selection. Moreover, results of correlation analysis indicated that CUB and various GC contents i.e. GC, GC1, GC2, GC3 played important role in the release of free energy by transcripts of the genes associated with pancreatic cancer. The low compAI values of coding sequences suggested a low translation rate of the genes.

Role of microRNA in forming breast carcinoma.

MicroRNA (miRNA) is a regulatory molecule which supervises various processes and gene expression. RNA polymerase II enzyme regulates miRNA gene transcription whereas miRNA biogenesis includes various proteins or enzymes. Starting from cell proliferation, cell cycle regulation and apoptosis are regulated by alteration in cancerous cells. Aberrant cell proliferation takes place and apoptosis is usually inhibited by oncogenic miRNA. The miRNA can be oncogenic or tumor suppressor in nature and their functions are opposite to each other. During tumorigenesis, the upregulation of oncogenic miRNA is mainly observed along with downregulation of tumor suppressors. The miRNA can degrade the target mRNA by using a slicer named argonaute protein (Ago) or by inhibiting the translation process. The miRNA may positively or negatively regulate the formation of tumor in breast by interfering with cellular activities. Thus, miRNA can be significantly used as a biomarker for monitoring breast cancer. In therapeutic intervention, the delivery of tumor suppressor miRNAs in a cancer patient can help in recovering from cancer. Different miRNAs regulate positively or negatively in these therapies. This review is mainly focused on the role of miRNA in breast carcinoma, its mechanisms and different therapies. It also includes miRNA biogenesis and different miRNAs involved in breast cancer.

Codon usage trend in genes associated with obesity.

Obesity is not only a social menace but also an economic burden as it reduces productivity and increases health care cost. We used bioinformatic tools to analyze the CUB of obesity associated genes and compared with housekeeping genes (control) to explore the similarities and differences between two data sets as no work was reported yet. The mean effective number of codons (ENC) in genes associated with obesity and housekeeping gene was 50.45 and 52.03 respectively, indicating low CUB. The relative synonymous codon usage (RSCU) suggested that codons namely CTG and GTG were over-represented in both obesity and housekeeping genes while under-represented codons were TCG, TTA, CTA, CCG, CAA, CGT, ATA, ACG, GTA and GCG in obesity genes and TCG, TTA, CCG, ATA, ACG, GTA, and GCG in housekeeping genes. t test analysis suggested that 11 codons namely TTA (Leu), TTG (Leu), CCG (Pro), CAC (His), CAA (Gln), CAG (Gln), CGT (Arg), AGA (Arg), ATA (Ile), ATT (Ile) and GCG (Ala) were significantly differed (p < 0.05 or p < 0.01) between obesity and housekeeping genes. Highly significant correlation was observed between GC12 and GC3 in obesity and housekeeping genes i.e. r = 0.580** and r = 0.498** (p < 0.01) respectively indicating the effect of directional mutation pressure present in all codon positions.