RESUMO
Poxviruses encode virulence factors that have been identified as proteins that are secreted from infected host cells. Some of these secretory proteins impede host immune defences. We have previously demonstrated that tanapox virus (TPV) infected cells secrete an early 38 kDa glycopeptide that binds to human (h) interferon-gamma, hIL-2, and hIL-5. We now show an additional activity in the supernatant from TPV infected cells that down-regulates the expression of tumour necrosis factor-alpha (TNF-alpha) induced cell adhesion molecule gene expression. This activity was not detected in mock infected cells. Enzyme linked immunosorbent assays (ELISA) on primary human endothelial cells, show the induction of E-selectin, vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) following TNF-alpha or IL-1 beta treatment, as expected. Supernatant from TPV infected cells significantly decreased the TNF-alpha but not IL-1 beta-induced expression of these molecules. Mobility shift assays and Northern blot analyses further show that the supernatant from TPV infected cells inhibited TNF-alpha-induced activation of the nuclear transcription factor-kappa B (NF-kappa B) and transcriptional activation of the E-selectin, VCAM-1 and ICAM-1 genes. Based on TNF-alpha affinity chromatography, this activity appears to be associated with a 38 kDa glycopeptide.
Assuntos
Moléculas de Adesão Celular/metabolismo , Fator de Necrose Tumoral alfa/fisiologia , Yatapoxvirus/patogenicidade , Animais , Aotidae , Northern Blotting , Moléculas de Adesão Celular/genética , Linhagem Celular/metabolismo , Linhagem Celular/virologia , Regulação para Baixo , Selectina E/genética , Selectina E/metabolismo , Endotélio Vascular , Ensaio de Imunoadsorção Enzimática , Humanos , Molécula 1 de Adesão Intercelular/genética , Molécula 1 de Adesão Intercelular/metabolismo , NF-kappa B/genética , NF-kappa B/metabolismo , Veias Umbilicais , Molécula 1 de Adesão de Célula Vascular/genética , Molécula 1 de Adesão de Célula Vascular/metabolismo , Virulência , Yatapoxvirus/imunologiaRESUMO
In a group of 34 industrial workers, chronically exposed to organophosphorus (OP) compounds, serum pseudocholinesterase activity was depressed significantly in the exposed group as compared to the control group. There was a significantly higher incidence of peripheral neuropathy among the workers exposed to OP compounds, as compared to the control group. Mild to profound sensorineural hearing deficits were detected in both the exposed and control groups. As the pre-exposure hearing status of the workers was not known and since many other factors can also cause pathological changes in the cochlear nerve, a definite conclusion about the ototoxic nature of the OP compounds could not be drawn.
Assuntos
Inseticidas/efeitos adversos , Doenças Profissionais/induzido quimicamente , Compostos Organofosforados/efeitos adversos , Adulto , Perda Auditiva Neurossensorial/sangue , Perda Auditiva Neurossensorial/induzido quimicamente , Humanos , Masculino , Pessoa de Meia-Idade , Doenças Profissionais/sangue , Doenças do Sistema Nervoso Periférico/sangue , Doenças do Sistema Nervoso Periférico/induzido quimicamente , Fatores de TempoRESUMO
One hundred forty-two patients with surgical complications of Ascaris lumbricoides were treated in our hospital over a period of 5 years. Included were 120 patients with subacute intestinal obstruction that were treated conservatively, and 22 patients with acute intestinal obstruction that required surgical intervention. Four of the 22 patients that were operated on died following various postoperative complications. However, there were no deaths among those presenting with subacute obstruction. In our experience, early recognition of the condition avoided serious complications and morbidity.
Assuntos
Ascaríase/complicações , Doenças do Íleo/etiologia , Obstrução Intestinal/etiologia , Doença Aguda , HumanosRESUMO
A male infant presented with two well-formed lower limbs and pelvis with external male genitalia attached to the thorax at the xiphisternum. The infant also had exomphalos major and talipes equinovarus deformity of the right foot. Surgical correction was done without any difficulty. The case is unusual, and such an abnormality has not been reported before.