RESUMO
[Figure: see text].
Assuntos
Coartação Aórtica/metabolismo , Pressão Arterial , Núcleo Celular/metabolismo , Células Endócrinas/metabolismo , Rim/metabolismo , Mecanotransdução Celular , Pressorreceptores/metabolismo , Renina/metabolismo , Animais , Coartação Aórtica/genética , Coartação Aórtica/patologia , Coartação Aórtica/fisiopatologia , Linhagem Celular , Núcleo Celular/genética , Núcleo Celular/patologia , Montagem e Desmontagem da Cromatina , Modelos Animais de Doenças , Células Endócrinas/patologia , Feminino , Homeostase , Integrina beta1/genética , Integrina beta1/metabolismo , Rim/patologia , Rim/fisiopatologia , Lamina Tipo A/genética , Lamina Tipo A/metabolismo , Masculino , Camundongos Knockout , Pressorreceptores/fisiopatologia , Renina/genética , Estresse MecânicoRESUMO
The acyl-CoA synthetase medium-chain family member 2 (Acsm2) gene was first identified and cloned by our group as a kidney-specific "KS" gene. However, its expression pattern and function remain to be clarified. In the present study, we found that the Acsm2 gene was expressed specifically and at a high level in normal adult kidneys. Expression of Acsm2 in kidneys followed a maturational pattern: it was low in newborn mice and increased with kidney development and maturation. In situ hybridization and immunohistochemistry revealed that Acsm2 was expressed specifically in proximal tubular cells of adult kidneys. Data from the Encyclopedia of DNA Elements database revealed that the Acsm2 gene locus in the mouse has specific histone modifications related to the active transcription of the gene exclusively in kidney cells. Following acute kidney injury, partial unilateral ureteral obstruction, and chronic kidney diseases, expression of Acsm2 in the proximal tubules was significantly decreased. In human samples, the expression pattern of ACSM2A, a homolog of mouse Acsm2, was similar to that in mice, and its expression decreased with several types of renal injuries. These results indicate that the expression of Acsm2 parallels the structural and functional maturation of proximal tubular cells. Downregulation of its expression in several models of kidney disease suggests that Acms2 may serve as a novel marker of proximal tubular injury and/or dysfunction.