Spatial discrimination of visually similar environments by hippocampal place cells in the presence of remote recalibrating landmarks.

Place cells in the rat hippocampus commonly show place-related firing activity in the animal's current environment. Here, we evaluated the capability of the place cell system to discriminate visually identical environments. Place cell activity was first recorded while rats moved freely in a cylinder divided into three connected sectors. Two sectors were visually identical whereas the third sector was made distinctive by the addition of visual and tactile cues. When in a given sector, the rats could not perceive the cues present in the other two sectors. Most cells had distinctive place fields in each sector, including the two identical sectors. To rule out the influence of non-controlled cues, rotations of the cylinder (+/- 120 degrees) were conducted. When successful, cylinder rotations resulted in equivalent field rotation for all cells. These results suggest that the place cell system is able to form a specific spatial representation for all sectors, so that the rat knows, at any time, in which sector it is currently located. Presumably, such discrimination relies on angular path integration in which the computational errors stemming from self-motion cues would be corrected by environmental landmarks provided by the distinctive sector.

Independent coding of connected environments by place cells.

Place cells are hippocampal neurons that have a strong location-specific firing activity in the rat's current environment. Collectively, place cells also provide a signature of the rat's environment as their ensemble activity is markedly different when recorded in distinct apparatuses. This phenomenon, referred to as 'remapping', suggests that each environment activates a different hippocampal map. In this study, we sought to determine the independence of such maps. In Experiment 1, we used a cylinder apparatus that was divided into two equal halves by a central barrier with an aperture allowing the rat to freely commute between the two sides. A local change in one side failed to induce field remapping in the changed side, thus precluding any significant conclusion to be drawn. We therefore designed Experiment 2 in which place cells were first recorded while rats explored three distinct high-walled boxes. Most cells had distinctive firing fields in each box. A runway was then added to connect two initially unrelated boxes. This manipulation altered the firing of some cells but the fields in each box were still clearly distinguishable. The final manipulation consisted of changing one box and allowing the rat to commute freely between the changed and unchanged boxes. While the firing fields remapped in the changed box, they were most usually unaltered in the unchanged box. These results suggest that the hippocampus holds a set of independent maps for each box, and that each specific map is activated mainly according to the rat's current sensory environment.