RESUMO
Adult Teladorsagia circumcincta survival and motility in vitro was examined in a range of different cell culture media, supplements and gas mixes. Under optimum conditions, worms survived for 14 days, exhibiting high motility for 9 days and egg production for 72 h. Optimum conditions involved co-culture of worms with a HeLa cell line in a supplemented cell medium (CEM) and an atmosphere containing 10% CO(2), 5% O(2) 85% N(2), 65% humidity at 37 degrees C. The incubation medium consisted of Minimum Essential Medium with 10% fetal calf serum, 1% non-essential amino acids, 1% glutamax and 1% penicillin-neomycin-streptomycin cocktail mix. Compared with optimum conditions, incubation in CEM alone, cell conditioned CEM, RPMI alone, Medium 199 alone, reduced CO(2) or O(2), or when cells were replaced with Escherichia coli, both survival and motility were reduced. Optimum conditions for adult T. circumcincta maintenance for culture, anthelmintic testing or generation of excretory/secretory products are described.
Assuntos
Trichostrongyloidea/crescimento & desenvolvimento , Animais , Dióxido de Carbono , Linhagem Celular , Técnicas de Cocultura , Meios de Cultura , Meios de Cultivo Condicionados , Feminino , Células HeLa , Humanos , Masculino , Movimento , Nitrogênio , Oviposição , Oxigênio , Ovinos , Fatores de Tempo , Trichostrongyloidea/fisiologiaRESUMO
From a series of 343 nerve biopsies taken by one clinician over a period of 12 years in Nepal, this paper describes the histopathological findings in 153 biopsies from 119 patients suffering from tuberculoid, borderline (dimorphous) or lepromatous leprosy, who were untreated at the time of first presentation and diagnosis. They were taken during the course of other studies, mainly concerned with the mode of transmission of leprosy, and which included biopsies of skin, dartos muscle, nasal mucous membrane and nipple, results of which have already been published. Examination of serial sections by light microscopy revealed a density of cellular infiltration in non-lepromatous cases, or of bacilli in macrophages and Schwann cells in lepromatous cases, which was marked in degree and usually widespread from one end of the biopsy to the other. Intraneural caseation was recorded in four patients with tuberculoid or borderline-tuberculoid leprosy, and many others in this part of the spectrum showed extensive disruption of perineurial and endoneurial structure. In lepromatous patients, the numbers of bacilli in the endoneurial area not infrequently exceeded one thousand per oil immersion field. Although well known to histopathologists familiar with this disease, it is considered that the significance of these findings, in patients presenting for the first time, is not well appreciated by those working in general medicine, neurology, epidemiology, or even in leprosy control.
Assuntos
Hanseníase/patologia , Nervos Periféricos/patologia , Feminino , Humanos , Antígeno de Mitsuda/administração & dosagem , Hanseníase/diagnóstico , Hanseníase/microbiologia , Masculino , Nepal , Nervos Periféricos/microbiologiaRESUMO
An account is given of the examination of the nasal mucus discharge smears for the presence of Myco. leprae in 322 untreated cases of leprosy seen during a period of one year of which 111 were cases of lepromatous leprosy and remaining 211 were borderline disease. The majority of the patients with lepromatous leprosy had leprosy bacilli in their nasal secretion, often in very great numbers, ans showed a high Morphological Index. Of 41 of the most active cases among the 211 patients with borderline leprosy, only one had Myco. leprae in the nasal mucus. Morphologically normal bacilli are no longer found in nose-blows after 6 months treatment with DDS. It is suggested that the nasal mucus provides a true index of infectivity, and that patients with borderline leprosy are in general not to be regarded as infectious
