In vitro susceptibility of chromoblastomicosis and phaehyphomycosis agents to antigungal drugs

The in vitro susceptibility of chromoblastomycosis and phaeohyphomycosis agents to antifungal drugs was appraised using the reference macrodilution method proposed by the National Committee for Clinical Laboratory Standards (NCCLS) for yeasts modified for filamentos fungi. The antifungal drugs amphotericin B, 5-fluorocytosine, intraconazole and fluconazole were tested agsints one environmental and 18 clinical isolates. This work amented the macrodilution methods proposed by NCCLS and suggests that a conidial suspension free of hyphae leads to a more reliable assay and provides for better reproducibility. The macrodilution method was performed with 10 (elevado ao 4) conidia ml-1. The MIC values ranged from 1.0 to 16.0 ug ml-1 for amphotericin B and 3.12 to 25.0 ug ml-1 for 5-fluorocytosine. A MIC range of 0.06 to 1.95 ug ml-1 was determined for itraconazole while 2.0 to 64.0 ug ml-1 was detected for fluconazole

Funçäo encefálica residual: problema para a confirmaçäo de morte encefálica em doadores potenciais de tecidos / Residual encephalic function: a problem to confirm the brain death in potential tissue donors patients

Os autores revisam os critérios clínicos e os exames complementares necessários para o diagnóstico de morte encefálica, assim como aspectos éticos, legais e morais ligados à questäo. Ressaltam que a avaliaçäo de um doador potencial objetiva o diagnóstico de morte encefálica em determinado momento, näo julgando seu prognóstico. Sugerem que cada centro médico envolvido com transplantes de tecidos desenvolva protocolo e crie arquivo de dados próprios