Diagnostic potential of urinary monocyte chemoattractant protein-1 for Alzheimer's disease and amnestic mild cognitive impairment.

BACKGROUND AND PURPOSE: The chemokine monocyte chemoattractant protein-1 (MCP-1) is involved in the pathogenesis of Alzheimer's disease (AD). This study aimed to investigate whether urinary MCP-1 can distinguish patients with AD, patients with amnestic mild cognitive impairment (aMCI) and cognitively normal (CN) subjects. METHODS: A total of 754 participants, including 97 patients with AD, 50 patients with aMCI and 84 age- and sex-matched CN controls as well as a cohort of 523 CN subjects of different ages, were enrolled from five hospitals located in different areas of China. Urinary MCP-1 levels were determined using enzyme-linked immunosorbent assays. The correlations between urinary MCP-1 levels and cognition test scores or age were analysed. The optimal diagnostic sensitivity and specificity were determined using receiver operating characteristic curve analysis. RESULTS: In the cohort of CN subjects of different ages, urinary MCP-1 levels increased with ageing and were correlated with age. The urinary MCP-1 levels were higher in females than in males. In the cohort composed of patients with AD, aMCI and age- and sex-matched CN controls, urinary MCP-1 levels were significantly higher in patients with AD and aMCI than in CN controls. There were no differences in urine MCP-1 levels between the AD group and the aMCI group. The urinary MCP-1 levels were correlated with the Mini-Mental State Examination scores and age, and were able to differentiate patients with AD and aMCI from CN subjects. CONCLUSIONS: Urinary MCP-1 is a potential biomarker for the diagnosis of AD and aMCI.

[The antibacterial efficacy to obligate anaerobes isolated from the infected root canal of deciduous teeth]

TMJ osteoarthrosis(OA) is frequently seen in the Oral and Maxillofacial Surgery clinics.In order to investigate cellular and molecular basis of the pathogenesis of TMJOA,the levels of IL-1 and IL-6 in synovial aspirates from different stages of TMJOA patients were determined and analysed.It was found that both of them were highly correlated with the severity of TMJOA(P<0.0001).A positive linear correlation was also found between IL-1 and IL-6(P<0.0001).The balance and imbalance between MMPs and TIMPS and the modulatory mechanism of cytokines were introduced and their possible roles in the pathogenesis of TMJOA were discussed.

[Culture of the sheep TMJ synovial cells in vitro-notes on cellular arrangement and ultrastructural feature]

The sheep TMJ synovial cells were cultured in virtro by tissue explantation.and the phase-contrast microscopic and electron microscopic examinations were made.The synovial cells in which they were arranged in a unique manner.It is suggested that the ultrastructural characteristics of the synovial cells in vitro can serve as the basis of cell-type classification.