RESUMO
OBJECTIVE: To investigate the effect of X-ray radiation at the median lethal dose (LD50) on the outcome of a cluster of differentiation 133 (CD133)- cells in nasopharyngeal carcinoma. MATERIALS AND METHODS: CD133- cells were obtained from human nasopharyngeal carcinoma cells (CNE-1 and CNE-2) based on CD133-labeled fluorescence-activated cell sorting (FACS) and magnetic-activated cell sorting (MACS), respectively. Changes in invasion ability and in-vivo tumorigenicity of CD133- cells before and after X-ray radiation at LD50 were observed. Moreover, CD133, SRY-related HMG-box 2 (SOX2), and organic carnitine transporter 4 (OCT4) expression changes were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. RESULTS: The invasion ability and in-vivo tumorigenicity of CD133+ cell subsets were significantly stronger than those of CD133- cell subsets. After X-ray radiation at LD50, the invasion ability of CD133- cell subsets and in-vivo tumorigenicity were significantly increased. RT-PCR and Western blotting results manifested that the expression levels of CD133, SOX2, and OCT4 were remarkably up-regulated after radiation. CONCLUSIONS: X-ray radiation at LD50 can enhance the stemness potential by up-regulating the expression of stemness-related genes in nasopharyngeal carcinoma CD133- cells.
Assuntos
Antígeno AC133/metabolismo , Regulação para Cima/efeitos da radiação , Raios X , Animais , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Humanos , Dose Letal Mediana , Camundongos , Camundongos Nus , Carcinoma Nasofaríngeo/metabolismo , Carcinoma Nasofaríngeo/patologia , Carcinoma Nasofaríngeo/radioterapia , Neoplasias Nasofaríngeas/metabolismo , Neoplasias Nasofaríngeas/patologia , Neoplasias Nasofaríngeas/radioterapia , Fator 3 de Transcrição de Octâmero/metabolismo , Fatores de Transcrição SOXB1/metabolismo , Transplante HeterólogoRESUMO
It was found that Ginsenoside B (GSB) could reduce the myocardial systolic power and frequency of the isolated atrium of guinea pigs in a concentration-dependent way, and obviously prolong the functional refractory period of the left atrium. GSB could competitively antagonize the positive inotropic action of Iso, and non-competitively antagonize the positive inotropic action of CaCl2 on the isolated right atrium of guinea pigs.
Assuntos
Bloqueadores dos Canais de Cálcio/farmacologia , Ginsenosídeos , Frequência Cardíaca/efeitos dos fármacos , Contração Miocárdica/efeitos dos fármacos , Período Refratário Eletrofisiológico/efeitos dos fármacos , Saponinas/farmacologia , Animais , Cloreto de Cálcio/antagonistas & inibidores , Depressão Química , Feminino , Cobaias , Átrios do Coração , Técnicas In Vitro , Isoproterenol/antagonistas & inibidores , MasculinoRESUMO
The experimental results show that the Maren soft capsule can increase the amount and weight of the stool of normal mice and model mice with dry stool, promote the advanced percentage of charcoal powder in the small and large intestines of mice, enhance the movement of the smooth muscle of isolated ileum of guinea pigs under physiological conditions or low temperature, as well as strengthen the intestinal movement in rabbits.