RESUMO
Resumo A pressão arterial é um parâmetro indispensável na monitorização anestésica. A mensuração da pressão artéria invasiva (iPA) mediante cateterização arterial periférica é confiável, mas requer experiência e pode desencadear complicações. A técnica oscilométrica não é invasiva (NiPA), só requer um manguito sobre uma artéria periférica e a mensuração é automatizada. Não obstante a acurácia da NiPA tem sido pouco explorada e, por tal motivo, este estudo objetivou comparar esta técnica com a iPA em ovelhas e cabras. Foram utilizadas 20 ovelhas e 20 cabras que seriam submetidas a laparoscopia sob anestesia geral. Uma vez em plano anestésico, foi cateterizada a artéria auricular caudal e conectada a um transdutor eletrônico de pressão acoplado a monitor multiparamétrico para mensuração da iPA, sistólica (S), diastólica (D) e média (M). Ao mesmo tempo um manguito de tamanho apropriado foi posicionado sobre a artéria radial e conectado ao mesmo monitor. A cada 5 minutos foram registradas as pressões arteriais por ambos os métodos, posteriormente, comparadas pelo teste Student, correlacionados pelo Pearson e avaliada a concordância pelo Bland-Altman. Nas ovelhas, a PAD foi semelhante e correlacionada entre NiPA e iPA (P=0,69; r=0,65; viés 0,7 ± 13,1 mmHg), já a PAS e PAM foram superestimadas pela NiPA. Em Cabras, a PAM resultou semelhante e correlacionada entre NiPA e iPA (P=0,566; r=0,57; viés 2,4 ± 20,2 mmHg), enquanto PAS foi superestimada e PAD subestimada. Conclui-se que, em ovelhas apenas a PAD e em cabras a PAM, mensuradas pelo método oscilométrico, são alternativas acuradas, factíveis e confiáveis para a monitoração anestésica da pressão arterial.
Abstract Blood pressure is an indispensable parameter in anesthetic monitoring. The measurement of invasive artery pressure (IBP) using peripheral arterial catheterization is reliable but requires experience and can lead to complications. The oscillometric technique is non-invasive (NIBP), it only requires a cuff over a peripheral artery and the measurement is automated. However, the accuracy of PANI has been little explored and, for this reason, this study aimed to compare this technique with PAI in sheep and goats. 20 sheep and 20 goats needed laparoscopy were used under general anesthesia. Once in anesthetic plane, the caudal auricular artery was catheterized and connected to an electronic pressure transducer, coupled to a multiparameter monitor to measure IBP, systolic (S), diastolic (D) and mean (M). At the same time, an appropriately sized cuff was placed over radial artery and connected to the same monitor. Every 5 minutes, blood pressures were recorded by both methods, subsequently compared by the Student test, correlated by Pearson and agreement assessed by the Bland-Altman. In sheep, DBP was similar and correlated between NIBP and IBP (P=0.69; r=0.65; bias 0.7 ± 13.1 mmHg), whereas SBP and MBP were overestimated by NIBP. In goats, MAP was similar and correlated between NIBP and IBP (P=0.566; r=0.57; bias 2.4 ± 20.2 mmHg), while SBP was overestimated and DBP underestimated. It is concluded that, in sheep only DBP and in goats, MBP, measured by the oscillometric method, are accurate, feasible and reliable alternatives for anesthetic monitoring of blood pressure.
Resumen La presión arterial es un parámetro indispensable en la monitorización anestésica. La medición de la presión arterial invasiva (PAI) mediante cateterismo arterial periférico es fiable, requiere experiencia y puede dar complicaciones. La técnica oscilométrica no es invasiva (PANI), requiere un manguito sobre una arteria periférica y medición automatizada. No obstante, la precisión de PANI ha sido poco explorada, por esta razón, este estudio tuvo por objetivo comparar esta técnica con la PAI en ovinos y caprinos. Se utilizaron 20 ovejas y 20 cabras que requerían laparoscopia bajo anestesia general. Una vez en plano anestésico, se cateterizó la arteria auricular caudal y se conectó a transductor de presión electrónico acoplado a monitor multiparamétrico para medir la PAI, sistólica (S), diastólica (D) y media (M). Al mismo tiempo, se colocó un manguito de tamaño adecuado sobre la arteria radial y se conectó al mismo monitor. Cada 5 minutos se registraron las presiones por ambos métodos, posteriormente se compraron por test de Student, correlacionaron por Pearson y se evaluó concordancia por Bland-Altman. En ovejas, la PAD fue similar y se correlacionó entre PANI y PAI (P=0,69; r=0,65; sesgo 0,7 ± 13,1 mmHg), mientras que PAS y MAP fueron sobreestimadas por PANI. En Cabras, la PAM fue similar y se correlacionó entre PANI y PAI (P=0,566; r=0,57; sesgo 2,4 ± 20,2 mmHg), mientras PAS se sobreestimó y PAD se subestimó. Se concluye que, en ovinos solo PAD y en caprinos PAM, medidas por el método oscilométrico son alternativas precisas, factibles y confiables para el monitoreo anestésico de la presión arterial.
RESUMO
Information on molecular mechanisms through which sex-steroids regulate oviductal function to support early embryo development is lacking. Here, we hypothesized that the periovulatory endocrine milieu affects the miRNA processing machinery and miRNA expression in bovine oviductal tissues. Growth of the preovulatory follicle was controlled to obtain cows that ovulated a small follicle (SF) and subsequently bore a small corpus luteum (CL; SF-SCL) or a large follicle (LF) and large CL (LF-LCL). These groups differed in the periovulatory plasmatic sex-steroid's concentrations. Ampulla and isthmus samples were collected on day four of the estrous cycle. Abundance of DROSHA, DICER1, and AGO4 transcripts was greater in the ampulla than the isthmus. In the ampulla, transcription of these genes was greater for the SF-SCL group, while the opposite was observed in the isthmus. The expression of the 88 most abundant miRNAs and 14 miRNAs in the ampulla and 34 miRNAs in isthmus were differentially expressed between LF-LCL and SF-SCL groups. Integration of transcriptomic and miRNA data and molecular pathways enrichment showed that important pathways were inhibited in the SF-SCL group due to miRNA control. In conclusion, the endocrine milieu affects the miRNA expression in the bovine oviduct in a region-specific manner.
Assuntos
Bovinos , Tubas Uterinas/efeitos dos fármacos , Hormônios Esteroides Gonadais/farmacologia , MicroRNAs , Animais , Bovinos/genética , Bovinos/metabolismo , Ciclo Estral/efeitos dos fármacos , Ciclo Estral/genética , Tubas Uterinas/metabolismo , Feminino , Perfilação da Expressão Gênica/veterinária , Regulação da Expressão Gênica/efeitos dos fármacos , MicroRNAs/efeitos dos fármacos , MicroRNAs/genética , MicroRNAs/metabolismo , Folículo Ovariano/efeitos dos fármacos , Folículo Ovariano/metabolismo , Ovulação/efeitos dos fármacos , Ovulação/genética , Processamento Pós-Transcricional do RNA/efeitos dos fármacos , Processamento Pós-Transcricional do RNA/genética , Transcriptoma/efeitos dos fármacosRESUMO
The aim was to compare the early luteal development in ewes superovulated with different doses of pFSH. Twenty-nine Santa Inês ewes received a progesterone device (CIDR®) for 8 days. Gonadotrophic treatment started on Day 6: G200 (control, n = 9, 200 mg); G133 (n = 10, 133 mg); and G100 (n = 10, 100 mg of pFSH). On Day 6, all females received eCG (300 IU). B-mode and spectral Doppler ultrasonography were performed daily during the early luteal phase (Days 11-15) to monitor the development of corpora lutea (CLs; dimensions) and ovarian arteries indices. CLs were also classified as normal or prematurely regressed (PRCL) on Day 15 by videolaparoscopy. Ewes from G100 and G133 showed gradual increase in luteal diameter during the early luteal phase (p < 0.001), whereas G200 animals presented increase from Day 11 to Day 13, and then decrease on Days 14 and 15 (p < 0.001). The G200 females showed greater percentage of PRCL (45.20%) than those of the other groups (p < 0.001). The normal CLs number was greater in G100 than in G133 (p = 0.04), while the PRCL number was greater in G200 than in the other groups (p = 0.03). Resistive index (RI) was greater in G200 than in G100 (p = 0.02). RI was lower in Day 12 than Day 15 (p = 0.02). Pulsatility index (PI) was greater on Days 14 and 15 (p < 0.01). In conclusion, the lowest dose of pFSH (100 mg) can be considered sufficient for an efficient superovulatory response in sheep, producing better CLs development dynamic in early luteal phase and ovarian blood perfusion and smaller number of PRCL than the traditional (200 mg) pFSH dose.
