Prevalence of radiographic appendicular osteoarthritis and associated clinical signs in young dogs.

This study aimed to determine the prevalence of osteoarthritis (OA) and associated clinical signs in young dogs. Owners of dogs aged 8 months-4 years from a single practice, were contacted in random order, to participate in a general health screen. Clinical and orthopedic examinations were performed. Each joint was scored for pain reactions (0-4). Orthogonal radiographs of all joints were made under sedation. Each joint was scored for radiographic OA (rOA) severity on an 11-point scale. Clinical OA (cOA) was defined as an overlap of rOA and joint pain in ≥ 1 joint. Owners completed OA questionnaires. The owners of 123 dogs agreed to participate. Overall, 39.8% (49/123) of dogs had rOA in ≥ 1 joint, and 16.3% (20/123) or 23.6% (29/123) dogs had cOA, depending on the cut-off value of joint pain; moderate (2), or mild (1), respectively. Owners of dogs with cOA observed signs of impairment in approximately 30% of cases. Only 2 dogs with cOA were receiving OA pain management. The most commonly affected joints in descending order of frequency were elbow, hip, tarsus, and stifle. Radiographically visible OA is common in young dogs, and 40-60% of dogs with rOA had cOA. However, OA-pain appears underdiagnosed and undertreated in young dogs.

Biocidal effects of a wipe-down procedure using common veterinary cleansers on microbial burden within working canine exterior coats.

Introduction: Recent work demonstrating reduction of aerosolized contamination via a wipe-down procedure using common veterinary antiseptics offers promise regarding health concerns associated with cross-contamination from working canines to humans. While mechanical reduction can be achieved via a wipe-down procedure, the biocidal impact on flora within the exterior coat is unknown. Methodology: This study assessed the biocidal impact of antiseptics on the exterior bacterial community of the canine. Lint-free towels were saturated with 2% chlorhexidine gluconate scrub, or 7.5% povidone-iodine scrub diluted at a 1:4 ratio. Treatments were rotated across the dorsal aspect of kennel housed Foxhounds (n = 30). Sterile swabs were collected in triplicate prior to, and following wipe down, stored in Amies solution at 4°C, plated onto nutrient agar and reduction in colony forming units (CFU) was measured across both treatments. Statistical analysis utilizing PROC GLM examined effects of treatment (p ≤ 0.05). Molecular analysis of the 16S rRNA gene was completed for 3 hounds. Results: Reduction in CFU was measured (p < 0.001) for both antiseptics. Qualitative molecular data indicated that both antiseptics had a biocidal effect on the dominant microbial community on the exterior coat with gram-positive, spore-forming taxa predominating post-treatment. Conclusion: Effective wipe-down strategies using common veterinary cleansers should be further investigated and incorporated to safeguard working canine health and prevent cross-contamination of human personnel.

Impacts to canine dermal microbiota associated with repeated bathing.

Introduction: Working dogs routinely operate in environmental conditions which may necessitate daily bathing to remove contaminants or soilage. The impacts of frequent or repeated bathing on the canine dermal microbiota are unknown. The objective of this study was to characterize changes in canine dermal microbial populations following repeated daily bathing. Methods: Labrador retrievers (n = 16) were bathed daily using a dilute dish detergent solution (1.6% detergent solution) over the course of 14 days. Dermal microbial DNA was collected via sterile swabs (n = 142) taken at days 0, 7, 14, 16, 21, 28, 35, 42, and 49 and analyzed for alpha diversity, beta diversity and relative abundance to assess changes in the dermal microbiota via 16 s sequencing. Results: Results indicate that daily bathing significantly increased Shannon diversity, Chao1, and several rare amplicon sequence variants. Although typically reported in highest abundance, relative abundance was decreased in the phyla Actinobacteria, Firmicutes, and Proteobacteria (p < 0.05). Conclusion: Repeated daily bathing with dilute dish detergent significantly reduced normal healthy dermal microbial taxa and created significant changes in the dermal microbiota of canines. Disruption to the canine dermal microbiota may cause negative impacts to canine dermal health and require further investigation.

An exploration of perceptions of online asynchronous and synchronous interprofessional education facilitation strategies.

Interprofessional education (IPE) has increasingly been delivered online using synchronous and asynchronous mediums; however, little research focuses on facilitation strategies in the synchronous environment. We explored whether the perceived strategies used by facilitators in online synchronous IPE facilitation are similar to those used in face-to-face and online asynchronous IPE facilitation, and if the strategies are used to a similar degree online in both synchronous and asynchronous environments. Following completion of an online IPE course, students and facilitators were invited to complete an anonymous questionnaire exploring their perceptions of the facilitation strategies used in their synchronous and asynchronous IPE experiences. Responses were received from 118 students and 21 facilitators. Descriptive statistics indicate that students and facilitators perceived facilitation strategies are being used in online synchronous settings that have previously been recognized in asynchronous and face-to-face IPE settings. This included strategies linked to communicating about the design/organization of the experience, providing direct instruction, facilitating and encouraging interprofessional interaction, and contextualizing IPE. Wilcoxon Signed-Rank tests demonstrated a perception that these strategies were being used to a greater extent in the synchronous than asynchronous environment. This knowledge is useful to further refine the training of online IPE facilitators in both synchronous and asynchronous settings.

An Assessment of Starch Content and Gelatinization in Traditional and Non-Traditional Dog Food Formulations.

Starch gelatinization in pet food may be affected by moisture, retention time, and ingredients used. Starch gelatinization has been associated with changes in digestibility but is not well studied using non-traditional ingredients in canine diets. The objective of this research was to examine differences in starch content and gelatinization associated with changes in ingredient profile (traditional vs. non-traditional) and nutrient content requirements associated with differing life stages. Traditional diets (n = 10) utilizing protein sources including chicken, chicken by-product meal, meat and bone meal and plant-based ingredients including rice, barley, oats, and corn were examined in comparison with non-traditional diets (n = 10) utilizing protein sources including alligator, buffalo, venison, kangaroo, squid, quail, rabbit, and salmon along with plant-based ingredients including tapioca, chickpeas, lentils, potato, and pumpkin. Total starch and gelatinized starch (as percent of total diet) were measured with variation due to ingredient type assessed using Student's t-test in SAS 9.4. Significance was set at p < 0.05. Total starch (as a percent of diet) was higher in traditional diets compared to non-traditional diets formulated for maintenance (p < 0.0032) or all life stages (p < 0.0128). However, starch gelatinization as a proportion of total starch was lower in traditional diets formulated for maintenance (p < 0.0165) and all life stages (p < 0.0220). Total starch and gelatinized starch had a strong negative correlation (r = −0.78; p < 0.01) in diets utilizing traditional ingredients. These novel data reveal important differences between starch content and gelatinization and may impact selection of various ingredient types by pet food manufacturers.

Influences of Oil-Based Palatants on Eating Behavior in Gestating Mares.

Gustatory and olfactory changes have been reported during gestation in multiple species. However, few data are available regarding feeding preferences in gestating mares. The objective of this research was to evaluate the effect of oil-based palatants on feeding behavior in late term gestating mares. Late-term gestating mares (n = 5) and reproductively sound, open mares (n = 4) were evaluated via paired preference testing during the final 120 days of gestation. Oil-based flavors (corn oil, anise, and apple) were topically applied to a basal pelleted diet (0.375 ml/0.23 kg). Diets were offered simultaneously using a ground level feeding platform and individual box stalls with 20 minutes allotted for engagement. Behaviors assessed included: sniffing, hovering, checking, chewing, chewing with the head lifted, uninterested, ingestion, pawing, flipping of feed pan, moving of feed platform, and defecation. Variables of interest included: first diet sniffed, consumed, and finished; time to finish first diet; time to finish all diets, and number of olfactory and consumption engagements for each diet. Data were analyzed using PROC FREQ and PROC MIXED in SAS Studio. Data analysis revealed a faster rate of consumption for first diet finished (P = .05) of gestating mares when compared to open mares. Additionally, gestating mares had a greater frequency of olfactory interactions across all diets (P < .01), but less consumption interactions across all diets (P = .01). These novel data suggest a previously unreported increase in olfactory investigation for gestating mares as well as new insights into gestational feeding behaviors of horses.

A Comparison of Canine Decontamination Cleansers: Implications for Water Use, Dermal pH, and Contaminant Reduction.

Environmental contamination is commonly experienced by working canines deployed in the field. Unfortunately, data regarding safety and efficacy of cleansers recommended for decontamination is lacking. Client-owned canines recruited from the community (n = 43) were randomly assigned to one of four treatment groups: povidone-iodine scrub [60mL Betadine® 7.5% povidone-iodine surgical scrub (Avrio Health L.P, Stamford, CT)], chlorhexidine scrub [60 mL Nolvasan® 2% chlorohexidine surgical scrub (Zoetis, Kalamazoo, MI)], dish detergent [60mL Dawn® dish detergent (Proctor & Gamble, Cincinnati, OH)], or water alone (control). A visual score assessing removal of a fluorescent marker (GloGerm, Moab, UT) applied between the shoulder blades was used to rate effectiveness of decontamination. Cleanser effect on canine dermal barrier function was determined by measuring pre- and post-decontamination dermal pH and trans-epidermal water loss (TEWL). Analysis of visual scores was performed using PROC FREQ and Chi Square. Significance was set a priori at 0.05 for all tests. Efficacy of fluorescent marker removal was significantly affected by cleanser (P<0.0001). Dermal pH was also highly affected by cleanser (P < 0.0001). In contrast, TEWL was unchanged across cleansers (P = 0.2686). Common veterinary cleansers utilized for canine decontamination demonstrate similarity in effectiveness for removal of a simulated contaminant and negative impact on dermal barrier function.

Responding to a Behavioral Health Crisis: Applying a New Care Model in the Emergency Department.

OBJECTIVES: Emergency department (ED) visits for behavioral health (BH) emergencies continue to rise, and institutions across the country encounter barriers and struggle to put BH processes in place to address their needs. After learning of an unanticipated closure of a local psychiatric crisis response center (CRC), our ED implemented quality improvement interventions to respond to an acute surge of BH patients. METHODS: Interventions included an enhanced BH database, the role of social workers as extenders, shared electronic health record documentation, increased staffing, clinical pathway updates, and processes to improve communication. We aimed to develop a care model to maintain safe care with timely evaluation and patient disposition despite an anticipated surge of ED patients. RESULTS: After the CRC closure, 7383 patients met our cohort definition over 18 months, whereas 4326 patients met the cohort definition in the 18 months prior the CRC closure. Of the total patients seen in the study period, 42% were evaluated by the ED team with psychiatry and social work, and the median length of stay for discharged patients evaluated by this team decreased from 4.2 hours to 3.5 hours after CRC closure. CONCLUSIONS: A multifaceted approach allowed our ED to successfully respond to an unexpected surge of BH patients. Other institutions may be able to apply a population health and quality improvement approach when addressing the rising prevalence of ED BH visits. Future studies and practices should explore the optimal role of the acute care setting in the continuum of care of these patients.

An assessment of working canine contamination from standing liquid hazards during a simulated disaster search scenario.

Working canines have the potential to be exposed to hazardous materials during search and rescue deployments. Unfortunately, little data are available regarding likely areas of contamination on working canines or effective techniques for substance removal. We describe recent work using an oil-based UV fluorescent marker pooled to mimic standing liquid hazards in a simulated disaster scene to characterize anatomical locations likely to be contaminated. This study utilized three simulated "contaminated" environments situated across a disaster training complex. Federal Emergency Management Agency (FEMA) working canines (n = 11) searched the simulated disaster scene and traversed each contaminated environment. Following the search, all canines were kenneled for 30 minutes and then photographed to capture anatomic locations of exposure. The canines were then taken immediately to the decontamination station where handlers' attempts at canine decontamination were recorded. Anatomical locations were coded as ventral or dorsal, and then further subdivided to the neck, chest, lower legs, and paws for ventral exposures; and back, head, face, and hips for dorsal exposures. Contamination occurred consistently on the paws and lower legs with overall ventral exposure occurring in 39 of 44 (89%) observations. Contamination of the back and head was infrequent, with overall dorsal exposure occurring in 11 of 44 (25%) observations. Despite handler awareness of the exact anatomical locations of exposure with a greater frequency of exposure involving ventral (78%) versus dorsal (22%) regions of the canine (P < 0.0001), time spent decontaminating the two regions did not differ (P = 0.881). These data indicate a need for additional research to identify effective decontamination techniques. Furthermore, the results suggest that additional training may be needed to educate handlers and veterinary personnel regarding anatomic locations on working canines likely to be contaminated during disaster operations in environments where standing liquid hazards are present.

Palatability of Horse Treats: Comparing the Preferences of Horses and Humans.

Despite its importance to product development, few data compare preferences for horses with human consumers. The objectives of this research were to compare treat preferences of horses against horse owners. Product A was a disk-shaped cinnamon-flavored flax-based treat, and product B was a textured apple-flavored oat-based treat. Horses were presented with two treat products in a paired preference test which comprised separate olfaction and consumption periods. Consumers evaluated the treats separately for purchase intent as well as hedonic testing of sensory attributes. No difference was observed for first product sniffed, consumed, or finished during the horse preference test. However, moderate positive correlations were observed between first product sniffed and consumed (P = .01, ф = 0.40) as well as first product consumed and finished (P < .01, ф = 0.48). Horse owners rated product A lower in appearance, texture, size, and purchase intent (P < .01) than product B. These results indicate that consumer testing for animal food should be considered during product development.

The Influence of Packaging on Palatability and Shelf Life Stability of Horse Treats.

Horse treat packaging may be composed of materials including plastic and paper which protect the product from the environment to improve shelf life. Objectives of this research were to 1) assess the impact of packaging on shelf life of horse treats and 2) evaluate the impact of packaging on horse preferences. Three packaging treatments (control, poly, and paper) were examined at five time points over a 12 month period. Treatments were analyzed for moisture, water activity, mold, yeast, pH, and volatile organic acids. Horse preference testing evaluated first treatment sniffed, consumed, and finished as well as number of treats consumed. Significance was set at P < .05 and trends at P < .10. Moisture content and water activity increased in all treatments (P < .01) from month 0 to month 12, with paper packaging providing a greater fluctuation and containing visible mold at month 12 (P < .01). No difference was observed for first treatment sniffed, consumed, or finished during preference testing. However a trend (P = .09) for the period∗treatment interaction was observed for number of treats consumed, with poly increasing while paper decreased. These data indicate that packaging impacts shelf life and horse preference of treats.

Removal of Aerosolized Contaminants from Working Canines via a Field Wipe-Down Procedure.

Evidence-based canine decontamination protocols are underrepresented in the veterinary literature. Aerosolized microbiological and chemical contaminants can pose a risk in deployment environments highlighting the need for improved canine field decontamination strategies. Prior work has established the efficacy of traditional, water-intensive methods on contaminant removal from the coat of the working canine; however, it is not known if similar reductions can be achieved with simple field expedient methods when resources are limited. The objective of this study was to measure the reduction of aerosolized contamination via a practical "wipe-down" procedure performed on working canine coats contaminated with a fluorescent, non-toxic, water-based aerosol. Disposable, lint-free towels were saturated with one of three treatments: water, 2% chlorhexidine gluconate scrub (CHX), or 7.5% povidone-iodine scrub (PVD). Both CHX and PVD were diluted at a 1:4 ratio. Treatments were randomly assigned to one of three quadrants established across the shoulders and back of commonly utilized working dog breeds (Labrador retrievers, n = 16; German shepherds, n = 16). The fourth quadrant remained unwiped, thus serving as a control. Reduction in fluorescent marker contamination was measured and compared across all quadrants. PVD demonstrated greater marker reduction compared to CHX or water in both breeds (p < 0.0001). Reduction was similar between CHX or water in Labradors (p = 0.86) and shepherds (p = 0.06). Effective wipe-down strategies using common veterinary cleansers should be further investigated and incorporated into decontamination practices to safeguard working canine health and prevent cross-contamination of human personnel working with these animals.

An Assessment of the Stability of the Canine Oral Microbiota After Probiotic Administration in Healthy Dogs Over Time.

The administration of an oral probiotic has been demonstrated to impact oral microbial diversity in humans but has not been examined in canines. The objective of this study was to test the hypothesis that oral probiotic administration would impact the oral microbiota of canines compared to control. Working canines in training (n = 13) were assigned to Test or Control groups and acclimated to one of three commercially available study diets utilizing common protein sources (Purina Pro Plan Savor lamb, Purina Pro Plan Sport chicken, Purina Pro Plan Focus salmon) for a minimum of 30 days prior to initiation of the study. Following acclimation, dogs in the Test group began a daily regimen of oral probiotic (Fortiflora® Purina, St. Louis, MO) top-dressed on their midday feeding. Control dogs received their midday feeding with no probiotic. All dogs were sampled once weekly via oral pediatric swabs across the 7-week study. Next generation sequencing (Illumina, MiSeq) was utilized to develop microbial profiles specific to treatment, diet, and time. Bacterial composition was dominated by eight phyla (Proteobacteria 43.8%, Bacteroidetes 22.5%, Firmicutes 18.9%, Actinobacteria 6.1%, Fusobacteria 3.6%, Gracilibacteria 2.1%, SR1 Absconditabacteria 1.5%, and Saccharibacteria 1.3%) representing more than 99% of the relative abundance of the microbial composition. Probiotic administration failed to impact relative abundance at any taxonomic level (P > 0.05). Similarly, no effect on the oral microbiota was measured for diet (P > 0.05). Comparison using a Jaccard Index demonstrate a consistent microbial profile over the 7-week study with no impact evidenced by study week (P = 0.19). The data also revealed a profile of ubiquitous taxa that were present across all dogs and all samples regardless of breed, sex, diet, treatment or other factors. These genera include Actinomyces, Corynebacterium, Capnocytophaga, Flavobacterium, Gemella, Abiotrophia, Streptococcus, and Frederiksenia. These data demonstrate the stability of canine oral microbiota over time.

The Influence of Topically Applied Oil-Based Palatants on Eating Behavior in Horses.

Palatants may be added to equine feed and medication either during or after manufacturing to enhance product acceptance. Prior studies have examined a variety of palatants but results have been limited and inconsistent. Therefore, the objective of this research was to evaluate topically applied oil-based palatants on feeding preferences in horses. Stock-type horses (n = 10) were used in this paired preference test across a two-phase study. Phase one compared six palatants (banana, anise, peppermint, apple, spearmint, and orange) to a control (corn oil), whereas phase two compared preference among palatants (anise, apple, and peppermint). Feeding stocks were utilized and horses were allowed 15 seconds for olfaction followed by 3 minutes for consumption. Variables recorded included first diet sniffed and consumed, first action, aversive behaviors, excessive salivation, and consumption. Each trial was also video-recorded and number of chews were counted. Data were analyzed using chi-square and t tests in SAS version 9.4 with P < .05 established as significant. Findings from phase one reveal excessive salivation was observed less frequently (P < .05) for most palatants with the exception of peppermint and orange. Orange negatively impacted palatability indicated by less consumption when compared with the control (P = .02), although there was no impact on chews per gram. No difference between control and treatment diets for first sniff or first consumed was observed when analyzed individually in either phase one or two, although there was a moderate positive correlation (ф = 0.39, P = .04) between olfaction and consumption during the peppermint and anise comparison. Consumption as the first action was consistent across all trials (P < .05). Anise was preferred over apple and peppermint as indicated by higher total consumption (P < .05) in phase two. These data indicate that oil-based palatants can affect feed preferences in horses with increased palatability from anise and decreased palatability from orange flavors.

An Assessment of Decontamination Strategies for Materials Commonly Used in Canine Equipment.

Working canines are frequently exposed to hazardous environments with a high potential for contamination. Environmental contamination may occur in many ways. Contamination may be chemical, biological, radiological, or nuclear. Examples may include a pipeline rupture following an earthquake, microbiological contamination of floodwaters, or exposure to toxic industrial chemical such as hydrogen chloride, ammonia, or toluene. Evidence to support effective methods for decontamination of equipment commonly used by working canines is lacking. Recent work has identified decontamination protocols for working canines, but little data are available to guide the decontamination of equipment used during tactical operations. The objective of our work was to investigate the effects of cleanser, cleaning method, and material type on contaminant reduction for tactical canine equipment materials using an oil-based contaminant as a surrogate for toxic industrial chemical exposure. A contaminant was applied, and effectiveness was represented as either success (= 50% contaminant reduction) or failure (< 50% contaminant reduction). A two-phase study was used to investigate cleanser, method of cleaning, and material types for effective contaminant reduction. In phase 1, Simple Green® cleanser had a higher frequency (P = .0075) of failure, but method and material did not affect contamination reduction (P > .05). In phase 2, Dawn® (P = .0004) and Johnson's® (P = .0414) successfully reduced contamination. High-pressure cleaning (HPC) resulted in successful decontamination (P < .0001). These novel data demonstrate potential techniques for reduction of contaminants on tactical canine equipment.

Body Temperature Responses During Phases of Work in Human Remains Detection Dogs Undergoing a Simulated Deployment.

Body temperature responses were recorded during phases of work (waiting-to-work in close proximity to search site, active work in a search site, and post-work recovery crated in vehicle) in human remains detection dogs during search training. State or federally certified human remains detection dogs (n = 8) completed eight iterations of searching across multiple novel search environments to detect numerous scent sources including partial and complete, buried, hidden, or fully visible human remains. Internal temperature (Tgi) of the body was measured continuously using an ingestible thermistor in the gastrointestinal tract. Mean total phase times were: waiting-to-work: 9.17 min (±2.27); active work: 8:58 min (±2:49); and post-work recovery: 24:04 min (±10.59). Tgi was impacted by phase of work (p < 0.001) with a small increase during active work, with mean peak temperature 39.4 °C (±0.34 °C) during that period. Tgi continued to increase for a mean of 6:37 (±6:04) min into the post-work recovery phase in the handler's vehicle with a mean peak Tgi of 39.66 °C (±0.41 °C). No significant increase in temperature was measured during the waiting-to-work phase, suggesting behaviors typical of anticipation of work did not appear to contribute to overall body temperature increase during the waiting-to-work recovery cycle. Continued increase of gastrointestinal body temperature several minutes after cessation of exercise indicates that risk of heat injury does not immediately stop when the dog stops exercising, although none of the dogs in this study reached clinically concerning body temperatures or displayed any behavioral signs suggestive of pending heat injury. More work is needed to better understand the impact of vehicle crating on post-work recovery temperatures in dogs.

Designing Solutions for Clean Water on Cape Cod: Engaging Communities to Improve Decision Making.

Many of the remaining mechanisms for reducing land-based nitrogen release in coastal communities depend on behavior change, social acceptance, and public support of localized mitigation programs. These needs necessitate appropriate and effective stakeholder engagement. Cape Cod, Massachusetts, USA, is one example of an area undergoing significant local, regional, state and federal decision-making processes to address nitrogen impacts on coastal waterbodies through an update to its Area Wide Water Quality Management Plan (208 Plan). The 208 Plan Update seeks to support mitigation of nitrogen pollution and restore estuarine health through active community engagement with elected officials, town staff, citizens, and other stakeholders across its 53 embayment watersheds, 35 of which are deemed impaired. With an economy deeply tied to the environment, the region is in the difficult position of needing to make significant infrastructure investments to maintain its reputation for high quality coastal waters. It is the first region in the United States to undergo an extensive revisit of its Area Wide Water Quality Management Plan developed pursuant to Section 208of the federal Clean Water Act for the purpose of addressing nitrogen. The community engagement process for the 208 Plan Update set forth to 1) understand the range of perspectives regarding the extent of the nitrogen impacts as well as the possible solutions, 2) ensure two-way communication of available information, and 3) build trust through a transparent process. The process specifically applied a number of different mechanisms for community engagement which enabled progress in addressing nitrogen management needs. The process helped to determine and address barriers to successful implementation of nitrogen mitigation plans and resulted in a framework for watershed-based planning that relies on regional coordination and supports local selection of mitigation strategies. As a result, communities in the region are developing innovative cross-municipal partnerships and committing to fund infrastructure necessary to decrease nitrogen loading to coastal embayments.

Handler training improves decontamination of working canines with oil-based exposure in field conditions using disposable kits.

Exposure to contaminants in the field is a reality for deployed canines. To date, there is no data evaluating the benefits of training for handlers associated with canine decontamination efforts. The objective of our work was to investigate the impact of handler training on the reduction of oil-based contaminants in working canines. Canine teams (n = 10) were randomly assigned to either TRAINED or UNTRAINED groups. Each team (handler and dog) in the TRAINED group received 30-minutes of interactive training using an illustrated guide on proper utilization of equipment provided. Teams in the UNTRAINED group received the same equipment and illustrated guide but no interactive training. Decontamination efforts were measured using an oil-based pseudo-contaminant (GloGerm®, Moab, UT) topically applied to four anatomical locations: cranial neck, between the shoulder blades, left medial hindlimb and left hind paw with pre- and post-washing images collected from a fixed distance of 20 inches. Visual assessment of contaminant reduction was scored as follows: 0 = <24% contaminant reduction; 1 = 25-50% contaminant reduction; 2 = 51-75% contaminant reduction; and 3 = >76% contaminant reduction. No score discrepancies >1 were reported between reviewers. Trained handlers were more effective at contamination reduction (P = .0093) as compared to their untrained counterparts. These results indicate that handlers, when properly trained, can achieve reduction of oil-based contaminants with a disposable decontamination kit and a garden hose.

When the Nose Doesn't Know: Canine Olfactory Function Associated With Health, Management, and Potential Links to Microbiota.

The impact of health, management, and microbiota on olfactory function in canines has not been examined in review. The most important characteristic of the detection canine is its sense of smell. Olfactory receptors are primarily located on the ethmoturbinates of the nasal cavity. The vomeronasal organ is an additional site of odor detection that detects chemical signals that stimulate behavioral and/or physiological changes. Recent advances in the genetics of olfaction suggest that genetic changes, along with the unique anatomy and airflow of the canine nose, are responsible for the macrosmia of the species. Inflammation, alterations in blood flow and hydration, and systemic diseases alter olfaction and may impact working efficiency of detection canines. The scientific literature contains abundant information on the potential impact of pharmaceuticals on olfaction in humans, but only steroids, antibiotics, and anesthetic agents have been studied in the canine. Physical stressors including exercise, lack of conditioning, and high ambient temperature impact olfaction directly or indirectly in the canine. Dietary fat content, amount of food per meal, and timing of meals have been demonstrated to impact olfaction in mice and dogs. Gastrointestinal (GI) microbiota likely impacts olfaction via bidirectional communication between the GI tract and brain, and the microbiota is impacted by exercise, diet, and stress. The objective of this literature review is to discuss the specific effects of health, management, and microbiota shifts on olfactory performance in working canines.

Effect of Road Transport on the Equine Cecal Microbiota.

The effects of travel stress on the equine cecal microbiota are poorly understood. We hypothesized that travel would affect the equine cecal microbiota. Cecally-cannulated horses (n = 6) were randomly assigned to one of two groups, travel (n = 3) and control (n = 3). Horses received a basal diet (Strategy, Purina Animal Nutrition) with 1.2% body weight mixed grass/alfalfa. Travel horses were transported to an unfamiliar location, stalled to simulate weekend horse show conditions, and then returned to the Southern Illinois University Equine Center. Control horses remained at the equine center for the entire study. Cecal fluid was collected on a 6-hour rotating schedule, four times daily throughout the 6-day study. Data were analyzed using mixed models in SAS with P < .05. Cecal bacterial DNA was extracted, followed by 16S RNA sequencing and then analyzed using QIIME 1.8.0. Averages of sequence data were reported by phase (baseline, transportation, post-travel). Although there were no effects of travel associated with ß-diversity (P > .05), analysis of α-diversity measures indicated an effect within the travel group during the transportation phase as compared with baseline (P < .05). Interestingly, α-diversity was also affected for control horses in the return phase when compared to baseline. This may be due to the disruption of the return of the travel group. In addition, we identified multiple taxa affected by travel at both the genus and phylum level. Continued profiling of equine gastrointestinal microbiota is necessary to improve our understanding of equine microbial dysbiosis.