Commercial hatchery practices have long-lasting effects on laying hens' spatial behaviour and health.

The commercial hatchery process is globally standardized and exposes billions of day-old layer chicks to stress every year. By alleviating this early stress, on-farm hatching is thought to improve animal welfare, yet little is known about its effects throughout production. This study compared welfare indicators and spatial behaviours during the laying period of hens hatched in an on-farm environment (OFH) to those hatched in a commercial hatchery and transferred at one day-old to a rearing barn (STAN). In particular, we assessed how OFH and TRAN hens differed in space-use and movement behaviours following the transfer to the laying barn at 17 weeks of age, a similar stressor encountered by STAN hens early in life, and determined whether effects aligned more with the 'silver-spoon' or 'environmental matching' hypothesis. We found that for the first three months post-transfer into the laying barn, OFH hens, on average, transitioned less between the aviary's tiers and spent less time on the littered floor. Because OFH hens became behaviourally more similar to STAN hens over time, these results suggest that OFH hens required a prolonged period to establish their daily behavioural patterns. Furthermore, OFH hens had more severe keel bone fractures throughout the laying period but similar feather damage and body mass to STAN hens. No differences were found in hen mortality or the number of eggs per live hen. These findings support the environmental matching hypothesis and suggest that early-life stressors may have prepared hens for later-life stressors, underscoring the importance of both early-life and adult environments in enhancing animal welfare throughout production.

Commercial laying hens exhibit long-term consistent individual differences and behavioural syndromes in spatial traits.

Past research has supported the importance of animal personalities for the productivity and welfare of farm animals. However, current assessments of personality traits are commonly conducted over short periods using standardized assays and may not reflect all important aspects of behaviours in commercial settings throughout the production period. This study aimed to evaluate consistent behavioural differences between 194 commercial laying hens within an aviary across most of the production period (eight months). We used five spatial behaviours related to various aspects of commercial hens' daily routine, including the sleeping, feeding, nesting, indoor movements and outdoor usage. All behaviours were repeatable over time and across contexts, with consistent differences between individuals explaining between 23% and 66% of the variation. These long-term consistencies revealed the potential applicability of the behaviours as personality traits of commercial hens. Moreover, we identified behavioural syndromes comprising all behaviours except the nesting-related behaviour, indicating two axes of spatial personalities that may be driven by different mechanisms. We discussed the significance of such individual differences in using personality traits to breed more resilient farm animals. Future research should evaluate associations of these behaviours with animal welfare and productivity to inform breeding efforts.

Intra-individual variation of hen movements is associated with later keel bone fractures in a quasi-commercial aviary.

Measuring intra- and inter-individual variation in movement can bring important insights into the fundamental ecology of animals and their welfare. Although previous studies identified consistent differences in movements of laying hens within commercial aviaries, the level of consistency was not quantified, limiting our capacity to understand the importance of individual movements for welfare. We aimed to quantify the scope of intra- and inter-individual differences in movements of commercial laying hens and examined their associations with indicators of welfare at the end of production. We quantified individual differences in one composite daily movement score for 80 hens over 54 days post-transfer to a quasi-commercial aviary. Results showed consistent inter-individual differences in movement averages, explaining 44% of the variation, as well as individual variation in predictability and temporal plasticity (at the population-level, hens increased their movements for 39 days). Hens that were more predictable in their daily movements had more severe keel bone fractures at the end of production while we found no such correlation between daily movement averages (individual intercept) and welfare indicators. Our findings highlight the importance of inter-individual difference in intra-individual variation of movements to improve poultry welfare.

Innovative problem-solving in a small, wild canid.

Innovation - the ability to solve problems in a novel way - is not only associated with cognitive abilities and relative brain size, but also by noncognitive traits, such as personality and motivation. We used a novel foraging task with three access options to determine how neophobia, exploration, and persistence influence innovation in 12 habituated bat-eared foxes (Otocyon megalotis) in the Kalahari Desert. Bat-eared foxes offer a unique system to understand cognition as they have the smallest relative brain size of measured canids and a specialized, termite-based diet, yet have displayed foraging innovations. Interestingly, most of our individuals solved the task at least once and six individuals solved the task in every trial. Neophobia did not influence success on the first trial, but both exploration and persistence influenced success across all trials. Those individuals that solved the puzzle over multiple trials became faster over time, suggesting that they learned how to open the box more efficiently. We found some variation in the method to open the puzzle box with six individuals solving the puzzle using two methods and one individual using all three methods. This is the first study to show innovation in a novel foraging task in wild bat-eared foxes.

Intra- and interspecific variation in self-control capacities of parrots in a delay of gratification task.

Forgoing immediate satisfaction for higher pay-offs in the future (delayed gratification) could be adaptive in situations that wild animals may encounter. To explain species-differences in self-control, hypotheses based on social complexity, feeding ecology, brain size and metabolic rate have been proposed. To explore these hypotheses in a comparative setting, we tested three macaw species (neotropical parrots)-great green macaws (N = 8), blue-throated macaws (N = 6), blue-headed macaws (N = 6)-and the distantly related African grey parrots (afrotropical parrots; N = 8) in a modified rotating tray task, in which subjects are required to inhibit consuming a constantly available low-quality reward in favour of a high-quality reward that becomes available only after an increasing delay (min. 5 s, max. 60 s). All four species successfully waited for a minimum of 8.3 s ± 11.7 s (group level mean ± SD) with African greys reaching a delay of 29.4 ± 15.2 s, and great green macaws-as best performing macaw species-tolerating delays of 20 s ± 8 s. The best performing African grey individual reached a maximum delay of 50 s, whereas, a great green and a blue-throated macaw tolerated a delay of 30 s max. Females tolerated higher maximum delays than males. Engaging in distraction behaviours enhanced waiting performance across species and all birds were able to anticipate the waiting duration. Our results suggest that both feeding and socio-ecological complexity may be a factor in self-control, but further systematically collected comparative data on self-control of different (parrot) species are required to test the evolutionary hypotheses rigorously.

Tameness does not correlate with the learning of an appetitive association in a wild canid.

Individual differences in cognition have been shown to be common in some animal taxa, and recent evidence suggests that an individual's personality can be associated with an individual's cognitive strategy. We tested whether wild bat-eared foxes Otocyon megalotis differ in a risk-taking behavior (tameness) and whether this trait correlated with appetitive association learning performance. While our result shows that individuals differed in their tameness, we found no association between this personality trait and learning the appetitive association. This result does not support the framework that differences in cognition are associated with differences in personality; however, our small sample size does not allow us to assert that personality cannot be associated with cognition in this system. This study highlights that measuring cognition and personality in wild systems presents added difficulty and that correlations found in captive animals may not be evident in their wild counterparts.

Oxytocin Experiments Shed Light on Mechanisms Shaping Prosocial and Antisocial Behaviors in Non-human Mammals.

Oxytocin has gained a reputation in popular culture as a simple "love drug" or "cuddle hormone", yet emerging biological evidence indicates that the effects of oxytocin are complex, mediating a suite of behavioral traits that range from ultrasocial to antisocial. Here we provide a comprehensive review to assess the salience of oxytocin in the lives of free-living social mammals. We reviewed the literature to understand the potential effects of oxytocin in promoting prosocial and antisocial behaviors in non-human mammals. Our review highlights a strong bias for studies of model organisms in highly-controlled settings, and emerging evidence for oxytocin's antisocial, context-specific and sex-specific effects. We discuss the results of the review in the context of insights gained from a pilot study aimed to investigate the potential for oxytocin to promote social cohesion in free-living yellow-bellied marmots (Marmota flaviventer). Our field experiment offers an example of the diverse issues that arise when conducting oxytocin manipulations in ecologically relevant contexts. Our synthesis highlights the challenges associated with acquiring adequate sample sizes for field-based, manipulative studies that require standardized measures of social behavior. Taken together, our findings lead us to join others in calling for revision of a simplistic view of oxytocin's role in regulating patterns of behavior. We draw from classical approaches used to study the mechanistic basis of behavior and offer a useful guide for disentangling these effects while appreciating the complex actions of oxytocin in shaping mammalian social behavior.

Social associations between California sea lions influence the use of a novel foraging ground.

Social relationships define an individual's position in its social network, which can influence the acquisition and spread of information and behavioural variants through the population. Thus, when nuisance behaviours spread through wildlife populations, identifying central individuals may provide valuable insights for problem-species management. We studied the effects of network position on California sea lion (Zalophus californianus) discovery and foraging success at a novel foraging ground-the salmonids that aggregate at the Bonneville Dam tail-race, 235 km up the Columbia River. We found that an individual's centrality in their social network influenced discovery of the Bonneville Dam and whether they returned the next year. Foraging success once at the dam was independent of network position. Extensive lethal and non-lethal removal efforts have been implemented at Bonneville Dam and focused on reducing the number of individual sea lions at the dam. Since social relationships forged at the opening of the Columbia River influence both the discovery and return to the Bonneville Dam, efforts to increase salmon recovery may be enhanced by breaking apart social networks at the opening of the river.

The effect of maternal glucocorticoid levels on juvenile docility in yellow-bellied marmots.

Maternal effects can have significant and long-term consequences on offspring behavior and survival, while consistent individual differences (i.e., personality) can have profound impacts on individual fitness. Thus, both can influence population dynamics. However, the underlying mechanisms that determine variation in personality traits are poorly understood. Maternal effects are one potential mechanism that may explain personality variation. We capitalized on a long-term study of yellow-bellied marmots (Marmota flaviventris) to identify maternal effects on juvenile docility. To do so, we partitioned the variance in juvenile docility using a quantitative genetic modeling approach to isolate potential maternal effects. We also directly tested whether maternal stress, measured through fecal glucocorticoid metabolite levels during lactation of 82 mothers, was associated with offspring docility. Docility scores were estimated for 645 juveniles trapped between 2002 and 2012. We found an interaction between maternal glucocorticoid levels and dam age on juvenile docility. We also found significant maternal, litter, permanent environment, and year effects. These results suggest that a mother's life history stage interacts with stress to influence offspring personality. This early life influence can have long lasting effects on an individual's docility throughout life.