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1.
Chembiochem ; 21(6): 836-844, 2020 03 16.
Artigo em Inglês | MEDLINE | ID: mdl-31566864

RESUMO

To unravel the underlying principles of membrane adaptation in small systems like bacterial cells, robust approaches to characterize membrane fluidity are needed. Currently available relevant methods require advanced instrumentation and are not suitable for high-throughput settings needed to elucidate the biochemical pathways involved in adaptation. We developed a fast, robust, and financially accessible quantitative method to measure the microviscosity of lipid membranes in bulk suspension using a commercially available plate reader. Our approach, which is suitable for high-throughput screening, is based on the simultaneous measurements of absorbance and fluorescence emission of a viscosity-sensitive fluorescent dye, 9-(2,2-dicyanovinyl)julolidine (DCVJ), incorporated into a lipid membrane. We validated our method using artificial membranes with various lipid compositions over a range of temperatures and observed values that were in good agreement with previously published results. Using our approach, we were able to detect a lipid phase transition in the ruminant pathogen Mycoplasma mycoides.


Assuntos
Corantes Fluorescentes/química , Ensaios de Triagem em Larga Escala , Lipídeos de Membrana/química , Mycoplasma mycoides/química , Tamanho da Partícula , Viscosidade
2.
Langmuir ; 34(49): 14921-14931, 2018 12 11.
Artigo em Inglês | MEDLINE | ID: mdl-30253101

RESUMO

DNA origami nanotechnology is being increasingly used to mimic membrane-associated biophysical phenomena. Although a variety of DNA origami nanostructures has already been produced to target lipid membranes, the requirements for membrane binding have so far not been systematically assessed. Here, we used a set of elongated DNA origami structures with varying placement and number of cholesteryl-based membrane anchors to compare different strategies for their incorporation. Single and multiple cholesteryl anchors were attached to DNA nanostructures using single- and double-stranded DNA spacers of varying length. The produced DNA nanostructures were studied in terms of their membrane binding and diffusion. Our results show that the location and number of anchoring moieties play a crucial role for membrane binding of DNA nanostructures mainly if the cholesteryl anchors are in close proximity to the bulky DNA nanostructures. Moreover, the use of DNA spacers largely overcomes local steric hindrances and thus enhances membrane binding. Fluorescence correlation spectroscopy measurements demonstrate that the distinct physical properties of single- and double-stranded DNA spacers control the interaction of the amphipathic DNA nanostructures with lipid membranes. Thus, we provide a rational basis for the design of amphipathic DNA origami nanostructures to efficiently bind lipid membranes in various environments.


Assuntos
Colesterol/análogos & derivados , DNA de Cadeia Simples/metabolismo , Nanoestruturas/química , Lipossomas Unilamelares/metabolismo , DNA de Cadeia Simples/química , Difusão , Ácidos Graxos Monoinsaturados/química , Estrutura Molecular , Conformação de Ácido Nucleico , Fosfatidilcolinas/química , Fosfatidilserinas/química , Polietilenoglicóis/química , Compostos de Amônio Quaternário/química , Lipossomas Unilamelares/química
3.
Biophys J ; 114(10): 2419-2431, 2018 05 22.
Artigo em Inglês | MEDLINE | ID: mdl-29706225

RESUMO

Fluorescent proteins (FPs) feature complex photophysical behavior that must be considered when studying the dynamics of fusion proteins in model systems and live cells. In this work, we characterize mNeonGreen (mNG), a recently introduced FP from the bilaterian Branchiostoma lanceolatum, in comparison to the well-known hydrozoan variants enhanced green fluorescent protein (EGFP) and Aequorea coerulescens GFP by steady-state spectroscopy and fluorescence correlation spectroscopy in solutions of different pH. Blind spectral unmixing of sets of absorption spectra reveals three interconverting electronic states of mNG: a nonfluorescent protonated state, a bright state showing bell-shaped pH dependence, and a similarly bright state dominating at high pH. The gradual population of the acidic form by external protonation is reflected by increased flickering at low pH in fluorescence correlation spectroscopy measurements, albeit with much slower flicker rates and lower amplitudes as compared to Aequorea GFPs. In addition, increased flickering of mNG indicates a second deprotonation step above pH 10 leading to a slight decrease in fluorescence. Thus, mNG is distinguished from Aequorea GFPs by a two-step protonation response with opposite effects that reflects a chemically distinct chromophore environment. Despite the more complex pH dependence, mNG represents a superior FP under a broad range of conditions.


Assuntos
Evolução Molecular , Proteínas de Fluorescência Verde/metabolismo , Animais , Concentração de Íons de Hidrogênio , Anfioxos , Espectrometria de Fluorescência
4.
Biointerphases ; 13(2): 028501, 2018 04 03.
Artigo em Inglês | MEDLINE | ID: mdl-29614862

RESUMO

Synthetic polymers, nanoparticles, and carbon-based materials have great potential in applications including drug delivery, gene transfection, in vitro and in vivo imaging, and the alteration of biological function. Nature and humans use different design strategies to create nanomaterials: biological objects have emerged from billions of years of evolution and from adaptation to their environment resulting in high levels of structural complexity; in contrast, synthetic nanomaterials result from minimalistic but controlled design options limited by the authors' current understanding of the biological world. This conceptual mismatch makes it challenging to create synthetic nanomaterials that possess desired functions in biological media. In many biologically relevant applications, nanomaterials must enter the cell interior to perform their functions. An essential transport barrier is the cell-protecting plasma membrane and hence the understanding of its interaction with nanomaterials is a fundamental task in biotechnology. The authors present open questions in the field of nanomaterial interactions with biological membranes, including: how physical mechanisms and molecular forces acting at the nanoscale restrict or inspire design options; which levels of complexity to include next in computational and experimental models to describe how nanomaterials cross barriers via passive or active processes; and how the biological media and protein corona interfere with nanomaterial functionality. In this Perspective, the authors address these questions with the aim of offering guidelines for the development of next-generation nanomaterials that function in biological media.


Assuntos
Transporte Biológico , Membrana Celular/metabolismo , Nanoestruturas/química , Polímeros/química , Polímeros/metabolismo , Modelos Biológicos , Modelos Químicos
5.
Nano Lett ; 18(5): 3185-3192, 2018 05 09.
Artigo em Inglês | MEDLINE | ID: mdl-29658275

RESUMO

We present a simple and versatile single-molecule-based method for the accurate determination of binding rates to surfaces or surface bound receptors. To quantify the reversible surface attachment of fluorescently labeled molecules, we have modified previous schemes for fluorescence correlation spectroscopy with total internal reflection illumination (TIR-FCS) and camera-based detection. In contrast to most modern applications of TIR-FCS, we completely disregard spatial information in the lateral direction. Instead, we perform correlation analysis on a spatially integrated signal, effectively converting the illuminated surface area into the measurement volume. In addition to providing a high surface selectivity, our new approach resolves association and dissociation rates in equilibrium over a wide range of time scales. We chose the transient hybridization of fluorescently labeled single-stranded DNA to the complementary handles of surface-immobilized DNA origami structures as a reliable and well-characterized test system. We varied the number of base pairs in the duplex, yielding different binding times in the range of hundreds of milliseconds to tens of seconds, allowing us to quantify the respective surface affinities and binding rates.

6.
Soft Matter ; 13(39): 7172-7187, 2017 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-28930355

RESUMO

Membrane-mediated interactions and aggregation of colloidal particles adsorbed to responsive elastic membranes are challenging problems relevant for understanding the microscopic organization and dynamics of biological membranes. We experimentally study the behavior of rodlike semiflexible fd virus particles electrostatically adsorbed to freestanding cationic lipid membranes and find that their behavior can be controlled by tuning the membrane charge and ionic strength of the surrounding medium. Three distinct interaction regimes of rodlike virus particles with responsive elastic membranes can be observed. (i) A weakly charged freestanding cationic lipid bilayer in a low ionic strength medium represents a gentle quasi-2D substrate preserving the integrity, structure, and mechanical properties of the membrane-bound semiflexible fd virus, which under these conditions is characterized by a monomer length of 884 ± 4 nm and a persistence length of 2.5 ± 0.2 µm, in perfect agreement with its properties in bulk media. (ii) An increase in the membrane charge leads to the membrane-driven collapse of fd virus particles on freestanding lipid bilayers and lipid nanotubes into compact globules. (iii) When the membrane charge is low, and the mutual electrostatic repulsion of membrane-bound virus particles is screened to a considerable degree, membrane-driven self-organization of membrane-bound fd virus particles into long linear tip-to-tip aggregates showing dynamic self-assembly/disassembly and quasi-semiflexible behavior takes place. These observations are in perfect agreement with the results of recent theoretical and simulation studies predicting that membrane-mediated interactions can control the behavior of colloidal particles adsorbed on responsive elastic membranes.


Assuntos
Bacteriófago M13/fisiologia , Membrana Celular/metabolismo , Membrana Celular/virologia , Vírion/química , Vírion/metabolismo , Bacteriófago M13/metabolismo , Bicamadas Lipídicas/metabolismo , Conformação Molecular
7.
Soft Matter ; 12(38): 7908-19, 2016 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-27492050

RESUMO

What are the physical laws of the mutual interactions of objects bound to cell membranes, such as various membrane proteins or elongated virus particles? To rationalise this, we here investigate by extensive computer simulations mutual interactions of rod-like particles adsorbed on the surface of responsive elastic two-dimensional sheets. Specifically, we quantify sheet deformations as a response to adhesion of such filamentous particles. We demonstrate that tip-to-tip contacts of rods are favoured for relatively soft sheets, while side-by-side contacts are preferred for stiffer elastic substrates. These attractive orientation-dependent substrate-mediated interactions between the rod-like particles on responsive sheets can drive their aggregation and self-assembly. The optimal orientation of the membrane-bound rods is established via responding to the elastic energy profiles created around the particles. We unveil the phase diagramme of attractive-repulsive rod-rod interactions in the plane of their separation and mutual orientation. Applications of our results to other systems featuring membrane-associated particles are also discussed.

8.
Biophys J ; 108(5): 1104-13, 2015 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-25762322

RESUMO

We study the effect of a minimal cytoskeletal network formed on the surface of giant unilamellar vesicles by the prokaryotic tubulin homolog, FtsZ, on phase separation in freestanding lipid membranes. FtsZ has been modified to interact with the membrane through a membrane targeting sequence from the prokaryotic protein MinD. FtsZ with the attached membrane targeting sequence efficiently forms a highly interconnected network on membranes with a concentration-dependent mesh size, much similar to the eukaryotic cytoskeletal network underlying the plasma membrane. Using giant unilamellar vesicles formed from a quaternary lipid mixture, we demonstrate that the artificial membrane-associated cytoskeleton, on the one hand, suppresses large-scale phase separation below the phase transition temperature, and, on the other hand, preserves phase separation above the transition temperature. Our experimental observations support the ideas put forward in our previous simulation study: In particular, the picket fence effect on phase separation may explain why micrometer-scale membrane domains are observed in isolated, cytoskeleton-free giant plasma membrane vesicles, but not in intact cell membranes. The experimentally observed suppression of large-scale phase separation much below the transition temperatures also serves as an argument in favor of the cryoprotective role of the cytoskeleton.


Assuntos
Proteínas de Bactérias/metabolismo , Proteínas do Citoesqueleto/metabolismo , Lipossomas Unilamelares/metabolismo , Proteínas de Bactérias/química , Proteínas do Citoesqueleto/química , Transição de Fase , Temperatura de Transição , Lipossomas Unilamelares/química
9.
Nano Lett ; 15(1): 649-55, 2015 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-25467421

RESUMO

We introduce a simple experimental system to study dynamics of needle-like nanoobjects in two dimensions (2D) as a function of their surface density close to the isotropic-nematic transition. Using fluorescence correlation spectroscopy, we find that translational and rotational diffusion of rigid DNA origami nanoneedles bound to freestanding lipid membranes is strongly suppressed upon an increase in the surface particle density. Our experimental observations show a good agreement with results of Monte Carlo simulations of Brownian hard needles in 2D.


Assuntos
DNA/química , Bicamadas Lipídicas/química , Modelos Químicos , Nanoestruturas/química , Método de Monte Carlo , Espectrometria de Fluorescência
10.
Phys Chem Chem Phys ; 16(5): 2020-37, 2014 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-24343177

RESUMO

Motivated by recent experimental observations of a rapid spontaneous DNA coil-globule transition on freestanding cationic lipid bilayers, we propose simple theoretical models for DNA condensation on cationic lipid membranes. First, for a single DNA rod, we examine the conditions of full wrapping of a cylindrical DNA-like semi-flexible polyelectrolyte by an oppositely charged membrane. Then, for two parallel DNA rods, we self-consistently analyze the shape and the extent of the membrane enveloping them, focusing on membrane elastic deformations and the membrane-DNA embracing angle, which enables us to compute the membrane-mediated DNA-DNA interactions. We examine the effects of the membrane composition and its charge density, which are the experimentally tunable parameters. We show that membrane-driven rod-rod attraction is more pronounced for higher charge densities and for smaller surface tensions of the membrane. Thus, we demonstrate that for a long DNA chain adhered to a cationic lipid membrane, such membrane-induced DNA-DNA attraction can trigger compaction of DNA.


Assuntos
DNA/química , Bicamadas Lipídicas/química , Modelos Moleculares , Cátions/química
11.
Faraday Discuss ; 161: 31-43; discussion 113-50, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23805736

RESUMO

Recently, DNA origami became a powerful tool for custom-shaped functional biomolecules. In this paper, we present the first approach towards assembling amphipathic three-dimensional DNA origami nanostructures and assessing their dynamics on the surface of freestanding phospholipid membranes. Our nanostructures were stiff DNA origami rods comprising six DNA helices. They were functionalized with hydrophobic cholesteryl-ethylene glycol anchors and fluorescently labeled at defined positions. Having these tools in hand, we could demonstrate not only the capability of the amphipathic nanorods to coat membranes of various phospholipid compositions, but also their switchable liquid-ordered/liquid-disordered partitioning on phase separated membranes. The observed translocation of our nanostructures between different domains was controlled by divalent ions. Moreover, selective fluorescent labeling enabled us to distinguish between the translational and rotational diffusion of our six helix bundles on the membranes by fluorescence correlation spectroscopy. The obtained data reveal how DNA origami can be employed as a valuable tool in membrane biophysics.


Assuntos
Membrana Celular/química , DNA/química , Nanoestruturas/química , Biofísica , Colesterol/análogos & derivados , Colesterol/química , Difusão , Etilenoglicóis/química , Interações Hidrofóbicas e Hidrofílicas , Conformação de Ácido Nucleico , Fosfatidilcolinas/química , Fosfolipídeos/química , Fosfolipídeos/metabolismo , Espectrometria de Fluorescência/métodos
12.
Langmuir ; 28(13): 5518-21, 2012 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-22424289

RESUMO

Giant unilamellar vesicles (GUVs) represent a versatile in vitro system widely used to study properties of lipid membranes and their interaction with biomacromolecules and colloids. Electroformation with indium tin oxide (ITO) coated coverslips as electrodes is a standard approach to GUV production. In the case of cationic GUVs, however, application of this approach leads to notorious difficulties. We discover that this is related to aging of ITO-coated coverslips during their repeated use, which is reflected in their surface topography on the nanoscale. We find that mild annealing of the ITO-coated surface in air reverts the effects of aging and ensures efficient reproducible electroformation of supergiant (diameter > 100 µm) unilamellar vesicles containing cationic lipids.


Assuntos
Eletroquímica/instrumentação , Bicamadas Lipídicas/química , Compostos de Estanho/química , Lipossomas Unilamelares/química , Eletrodos
13.
Biophys J ; 102(3): 523-31, 2012 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-22325275

RESUMO

Evidence has accumulated that the voltage-dependent anion channel (VDAC), located on the outer membrane of mitochondria, plays a central role in apoptosis. The involvement of VDAC oligomerization in apoptosis has been suggested in various studies. However, it still remains unknown how exactly VDAC supramolecular assembly can be regulated in the membrane. This study addresses the role of lipids in this process. We investigate the effect of cardiolipin (CL) and phosphatidylglycerol (PG), anionic lipids important for mitochondria metabolism and apoptosis, on VDAC oligomerization. By applying fluorescence cross-correlation spectroscopy to VDAC reconstituted into giant unilamellar vesicles, we demonstrate that PG significantly enhances VDAC oligomerization in the membrane, whereas cardiolipin disrupts VDAC supramolecular assemblies. During apoptosis, the level of PG in mitochondria increases, whereas the CL level decreases. We suggest that the specific lipid composition of the outer mitochondrial membrane might be of crucial relevance and, thus, a potential cue for regulating the oligomeric state of VDAC.


Assuntos
Fosfolipídeos/metabolismo , Multimerização Proteica , Canal de Ânion 1 Dependente de Voltagem/química , Animais , Apoptose , Cardiolipinas/metabolismo , Bovinos , Membrana Celular/química , Membrana Celular/metabolismo , Detergentes/química , Humanos , Mitocôndrias/metabolismo , Modelos Moleculares , Fosfatidilgliceróis/metabolismo , Estrutura Quaternária de Proteína , Espectrometria de Fluorescência , Lipossomas Unilamelares/química , Lipossomas Unilamelares/metabolismo
14.
Biophys J ; 100(1): 80-9, 2011 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-21190659

RESUMO

We address the relationship between membrane microheterogeneity and anomalous subdiffusion in cell membranes by carrying out Monte Carlo simulations of two-component lipid membranes. We find that near-critical fluctuations in the membrane lead to transient subdiffusion, while membrane-cytoskeleton interaction strongly affects phase separation, enhances subdiffusion, and eventually leads to hop diffusion of lipids. Thus, we present a minimum realistic model for membrane rafts showing the features of both microscopic phase separation and subdiffusion.


Assuntos
Membrana Celular/metabolismo , Citoesqueleto/metabolismo , Transição de Fase , Difusão , Dimiristoilfosfatidilcolina/metabolismo , Lipídeos de Membrana/metabolismo , Fosfatidilcolinas/metabolismo
15.
Biophys J ; 95(1): 390-9, 2008 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-18339763

RESUMO

Fluorescence correlation spectroscopy with total internal reflection excitation (TIR-FCS) is a promising method with emerging biological applications for measuring binding dynamics of fluorescent molecules to a planar substrate as well as diffusion coefficients and concentrations at the interface. Models for correlation functions proposed so far are rather approximate for most conditions, since they neglect lateral diffusion of fluorophores. Here we propose accurate extensions of previously published models for axial correlation functions taking into account lateral diffusion through detection profiles realized in typical experiments. In addition, we consider the effects of surface-generated emission in objective-based TIR-FCS. The expressions for correlation functions presented here will facilitate quantitative and accurate measurements with TIR-FCS.


Assuntos
Transferência Ressonante de Energia de Fluorescência/instrumentação , Transferência Ressonante de Energia de Fluorescência/métodos , Espectrometria de Fluorescência/instrumentação , Espectrometria de Fluorescência/métodos , Desenho de Equipamento , Análise de Falha de Equipamento
16.
Biophys J ; 94(5): L41-3, 2008 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-18192354

RESUMO

The Saffman-Delbrück approximation is commonly used in biophysics to relate the membrane inclusion size to its translational diffusion coefficient and membrane viscosity. However, this approximation has a restricted validity range, and its application to determination of inclusion sizes from diffusion data may in certain cases lead to unreliable results. At the same time, the model by Hughes et al. (Hughes, B. D., B. A. Pailthorpe, and C. R. White. 1981. J. Fluid Mech. 110:349-372.), providing diffusion coefficients of membrane inclusions for arbitrary inclusion sizes and viscosities of the membrane and surrounding fluids, involves substantial computational efforts, which prevents its use in practical data analysis. We develop a simple and accurate analytical approximation to the Hughes et al. model and demonstrate its performance and utility by applying it to the recently published experimental data on translational diffusion of micrometer-sized membrane domains.


Assuntos
Algoritmos , Fluidez de Membrana , Lipídeos de Membrana/química , Membranas Artificiais , Membrana Celular/química , Membrana Celular/metabolismo , Difusão , Lipídeos de Membrana/metabolismo , Modelos Teóricos , Tamanho da Partícula , Viscosidade
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