RESUMO
The study concerned an influence of saline content of liquid phase of crushed ore and presence or absence of solution blending on the process of cyanic hydrogen release in air of working area gold-mining industry.
Assuntos
Meio Ambiente , Ouro , Cianeto de Hidrogênio/efeitos adversos , Cianeto de Hidrogênio/metabolismo , Mineração/estatística & dados numéricos , Doenças Profissionais/epidemiologia , Doenças Profissionais/etiologia , Humanos , Estudos RetrospectivosRESUMO
A diagnostic kit for the determination of IgG antibodies to diphtheria toxin ("Abion", Germany) the use of analytical columns "Abicap" was studied in the specialized laboratory of the Tarasevich State Control Institute with a view to determine the possibility of its official registration in the Russian Federation. The kit was evaluated by the following criteria: the correspondence of the specified properties of the preparation to those actually observed; meeting the norms stated in the documents supplied with the kit and WHO requirements; the presence of advantages in comparison with the enzyme immunoassay. In the course of the trial positive results were obtained on all these points.
Assuntos
Toxina Diftérica/imunologia , Técnicas Imunoenzimáticas/normas , Imunoglobulina G/análise , Testes Sorológicos/normas , Humanos , Kit de Reagentes para Diagnóstico , Padrões de Referência , Reprodutibilidade dos Testes , Federação Russa , Estatística como Assunto , Organização Mundial da SaúdeRESUMO
In this article materials on obtaining rickettsial liposomal preparations on the basis of R.prowazekii phospholipid antigen and chemical typhus vaccine are presented. Electron microscopic studies revealed the formation of liposomes in this system. Liposomal preparations were not hemotoxic and produced no toxic action in experiments on guinea pigs and white mice. Phospholipid antigens incorporated into liposomal preparations were found to produce a potentiating effect on the immunogenic properties of chemical typhus vaccine. The adjuvant properties of rickettsial phospholipids incorporated into liposomes on the basis chemical typhus vaccine were registered.
Assuntos
Antígenos de Bactérias/isolamento & purificação , Fosfolipídeos/imunologia , Rickettsia prowazekii/imunologia , Animais , Anticorpos Antibacterianos/biossíntese , Vacinas Bacterianas/imunologia , Cobaias , Imunidade Celular , Lipossomos , Camundongos , Microscopia Eletrônica , CoelhosRESUMO
The technology of the purification of C.burnetii from the yolk-sac components of the cultivation medium, including differential centrifugation, membrane filtration, enzymatic hydrolysis, the standardization of the preparation thus obtained and its stabilization by the method of lyophilization, was developed. Antigens, prepared in accordance with this technology, were sufficiently active and highly specific. The possibility of their use in different serological tests (the complement fixation test and the indirect fluorescent analysis) was studied. In addition, these antigens were found to have good capacity for sorption on the solid-phase carrier (plates) and to be suitable for use in such highly sensitive test as the enzyme immunoassay.
Assuntos
Biotecnologia , Coxiella burnetii/isolamento & purificação , Animais , Centrifugação , Embrião de Galinha , Testes de Fixação de Complemento , Meios de Cultura , Filtração , Técnica Indireta de Fluorescência para Anticorpo , Liofilização , Hidrólise , Sensibilidade e Especificidade , Testes SorológicosRESUMO
To evaluate a kit for the enzyme immunoassay (ELISA), the metrological approach was used: the total error of the method for the quantitative determination of antibodies, regarded as a multistage process, was determined as the result of the accumulation of errors made in measurements at different stages. The proposed algorithm made it possible to attest the positive control serum, to determine the total error of measurements, to mark a linear section in a limited range of values on a graduation diagram. This led to obtaining well-grounded results, comparable with the results of the reaction of neutralization at good correlation (lc = 0.9).
Assuntos
Algoritmos , Anticorpos Antibacterianos/análise , Antitoxina Diftérica/imunologia , Ensaio de Imunoadsorção Enzimática , Antitoxina Tetânica/imunologia , Estudos de Avaliação como Assunto , Humanos , Modelos Lineares , Reprodutibilidade dos TestesRESUMO
Species-specific antigenic erythrocyte diagnosticum (SAED) for the detection of antibodies to R.prowazekii was developed. The cells used as the basis for the preparation of the antigenic erythrocyte diagnosticum were formulated and tanned sheep red blood cells (FT-SRBC). Original indication antigens, solubilized with Triton X-100 from common R.prowazekii antigen for the complement fixation test, the antigen neutralization test and the fluorescent analysis, were used as sensitin. The optimum parameters for sensitizing FT-SRBC were established. The SAED thus obtained was stabilized by the method of lyophilization. In a separate series of experiments the specificity and activity of SAED were studied in comparison with those of preparations obtained with the use of different sensitins. The newly developed SAED was shown to be highly specific.
Assuntos
Anticorpos Antibacterianos/sangue , Eritrócitos/imunologia , Rickettsia prowazekii/imunologia , Testes Sorológicos/métodos , Especificidade de Anticorpos , Testes de Hemaglutinação , Humanos , Técnicas de Imunoadsorção , Especificidade da EspécieRESUMO
In this article the problems of immunodiagnostics of generalized and local chlamydial infections are discussed. Extensive material is used for the substantiation of the expediency of using serological methods for diagnosing zoonotic and anthroponotic chlamydial infections. A rational scheme of using the passive hemagglutination (PHA) test and the complement fixation test for the detection of generalized chlamydial infections, as well as the PHA test and the indirect immunofluorescence test for diagnosing urogenital chlamydial infections.
Assuntos
Doenças das Aves/diagnóstico , Infecções por Chlamydia/diagnóstico , Infecções por Chlamydia/veterinária , Testes Sorológicos/métodos , Testes Sorológicos/veterinária , Doenças dos Ovinos/diagnóstico , Doenças dos Suínos/diagnóstico , Animais , Bovinos , Galinhas , Columbidae , Testes de Fixação de Complemento/veterinária , Doenças Urogenitais Femininas/diagnóstico , Doenças Urogenitais Femininas/veterinária , Técnica Indireta de Fluorescência para Anticorpo/veterinária , Testes de Hemaglutinação/veterinária , Humanos , Doenças Urogenitais Masculinas , Ovinos , Suínos , ZoonosesRESUMO
The methods for obtaining antigenic and immunoglobulin preparations intended for the rapid serological diagnosis of coxiellosis were developed. The cells used as the basis for their preparation were formulated tanned sheep red blood cells. As sensitin, rabbit anti-C.burnetii immunoglobulin was used. The capacity for the detection of C.burnetii antigens in the passive hemagglutination test and specific antibodies in the passive agglutination test with the use of the our immunoglobulin erythrocytic diagnosticum was studied. To construct coxiellosis antigenic erythrocytic diagnosticum, the complex of membrane antigens obtained from C.burnetii strain M-44 was used as sensitin. The positive results of the approbatory trial of the erythrocytic diagnosticums made it possible to recommend both preparations for use in laboratory practice in the system of conjugated unidirectional serological reactions.
Assuntos
Antígenos de Bactérias/sangue , Coxiella burnetii/imunologia , Eritrócitos/imunologia , Imunoglobulinas/sangue , Febre Q/diagnóstico , Testes Sorológicos/métodos , Animais , Febre Q/imunologia , OvinosRESUMO
The influence of vermin of immunity was studied in experiments on mice. This preparation was shown to have immunomodulating action. Of three doses under test (0.0025, 0.25 and 25 mg/kg), the preparation used in the first two doses exhibited immunostimulating action and in the third dose, immunosuppressing action. The realization of the immunotropic effects of vermin was linked with the influence of the preparation of B-cell-mediated immunity and the synthesis of antibodies.
Assuntos
Adjuvantes Imunológicos/uso terapêutico , Anelídeos/química , Produtos Biológicos/uso terapêutico , Imunidade/efeitos dos fármacos , Animais , Formação de Anticorpos/efeitos dos fármacos , Linfócitos B/efeitos dos fármacos , Biomassa , Relação Dose-Resposta a Droga , Avaliação Pré-Clínica de Medicamentos , Feminino , Masculino , CamundongosRESUMO
The investigations were performed during 139 operations. A conclusion was made that the arterial pressure level in the intramural vessels of the intestine was a reliable criterion of its viability. Repeated measures after the liquidation of the intestine strangulation can show the reestablishment of the disturbed blood circulation. The angiotensometry indices can serve as a guide for surgical tactics.