Subgingival 0.75% boric acid vs 1% povidone-iodine adjunctive to subgingival instrumentation in stage II and III periodontitis-A double-blind randomized clinical trial.

PURPOSE: To compare the effects of subgingival irrigation with 0.75% boric acid (BA) and 1% povidone-iodine (PVP-I) as an adjunct to scaling and root planing (SRP) on clinical and microbiologic parameters in the management of patients with periodontitis after a 12-month follow-up. METHODS: Sixty systemically healthy individuals diagnosed with periodontitis were included in this double-blind randomised clinical trial. The patients were randomly allocated to treatment groups: (1) SRP plus 0.75% BA and (2) SRP plus 1% PVP-I. Whole-mouth periodontals were clinically examined, and the counts of bacteria including Aggregatibacter actinomycetemcomitans (Aa), Fusobacterium nucleatum (Fn), Porphyromonas gingivalis (Pg), Treponema denticola (Td), Tannerella forsythia (Tf), Solobacterium moorei (Sm) and Streptococcus salivarius (Ss) were tested by real-time polymerase chain reaction (PCR). RESULTS: All periodontal parameters and the counts of Aa, Fn, Pg, Td, Tf, Sm and Ss in both groups showed statistically significant reductions at T3, T6 and T12 compared to T0. Whole-mouth or moderate or severe PD and CAL improvements were significantly found in the 0.75% BA group compared to the 1% PVP-I group at T3, T6 and T12. The reduction in Aa or Fn and the reduction in Ss were significantly higher in the 0.75% BA group at T6 and T12 than in the 1% PVP-I group. CONCLUSION: This study shows that subgingival irrigation with 0.75% BA may be an alternative to 1% PVP-I because it promotes greater PD reductions and CAL gain, particularly up to 12 months after treatment.

Comparison of Antimicrobial Activity against Porphyromonas gingivalis between Advanced Platelet-Rich Fibrin and Injectable Platelet-Rich Fibrin.

Platelet-rich fibrin (PRF) obtained via low-speed centrifugation has antimicrobial properties. This study was conducted to evaluate the effectiveness of advanced platelet-rich fibrin plus (A-PRF+) and injectable platelet-rich fibrin (I-PRF), obtained from patients with different periodontal states, against Porphyromonas gingivalis. A-PRF+ and I-PRF samples were obtained from venous blood of 60 subjects divided equally into three groups: periodontitis, gingivitis, and healthy gingiva groups. The antibacterial experiments evaluated biofilm inhibition, mature biofilm impact, and time-kill kinetics. The percent reduction in biofilm-growing and mature biofilm bacteria ranged from 39% to 49% and 3% to 7%, respectively. In the time-kill kinetics assay, PRF from the periodontitis group was more effective as an antimicrobial than that from the gingivitis and healthy gingiva group (p < 0.001); I-PRF was more effective than A-PRF+ (p < 0.05) and both of them showed peak antibacterial activity after 12 h of exposure. Both A-PRF+ and I-PRF exhibited antibacterial properties against P. gingivalis, but I-PRF appeared to be more effective. The PRF obtained from the different groups appeared to have different degrees of antimicrobial efficacy.

Antimicrobial effect against Aggregatibacter actinomycetemcomitans of advanced and injectable platelet-rich fibrin from patients with periodontal diseases versus periodontally healthy subjects.

Objectives: Advanced platelet-rich fibrin (A-PRF+) and injectable platelet-rich fibrin (i-PRF) have recently been developed and used in periodontal therapy. Few studies have contrasted the antibacterial effectiveness of these autologous materials derived from individuals with healthy gums, gingivitis, and periodontitis. This study aimed to compare the antimicrobial effects of these PRF materials against the periodontal pathogenic bacterium Aggregatibacter actinomycetemcomitans (Aa) in patients with different periodontal conditions. Methods: Blood samples were collected from periodontally healthy individuals, patients with gingivitis, or patients with periodontitis to prepare A-PRF+ and i-PRF. The antibacterial capacity of these materials was evaluated through antibiofilm formation, biofilm susceptibility, and the time-kill assay over a 48-h period. Results: A-PRF+ and i-PRF from each patient groups interfered with Aa's ability to form biofilm on the test tube surface, and the effect of i-PRF was significantly different among the patient groups. In contrast, these plasma preparation had a weak impact on mature biofilm. For products from the gingivitis and periodontitis groups, these effects were significantly stronger for i-PRF than A-PRF+ (p = 0.012 and p = 0.004, respectively). All plasma preparations inhibited Aa growth in the first 12 h after application, and i-PRF exhibited a significantly greater antimicrobial effect than A-PRF + at each time point. Conclusion: A-PRF+ and i-PRF in all three patient groups could inhibit the growth of Aa in vitro, and i-PRF from patients with periodontitis exhibited a more significant effect than PRF from the other groups.

Nonsurgical periodontal treatment improved the type 2 diabetes mellitus status in smokers: A randomized controlled trial.

AIM: Type 2 diabetes mellitus (T2D) and periodontal disease have bilateral associations. The effect of periodontal treatment on T2D patients who smoke is scarce. This study aimed to assess the effect of nonsurgical periodontal treatment (NSPT) in periodontitis smokers with T2D for a duration of 6 months of follow-up. MATERIALS AND METHODS: Forty moderate to severe periodontitis smokers with T2D were randomly distributed into two different treatment groups: the test group (NSPT including oral hygiene instructions, scaling and root planing; and 0.05% Chlorhexidine mouthrinse) and the control group (treatment including oral hygiene instructions, supragingival removal of plaque and calculus and 0.05% Chlorhexidine mouthrinse). Periodontal parameters including plaque index (PI), gingival index (GI), bleeding on probing (BOP), periodontal probing depth (PPD) and clinical attachment loss (CAL) were examined. Metabolic parameters, including fasting plasma glucose (FPG) and glycated hemoglobin (HbA1c), and high-sensitivity C-reactive protein (hs-CRP) were evaluated at baseline and at 1, 3, and 6 months of follow-up. RESULTS: The test group significantly improved all periodontal parameters and reduced metabolic parameters and hs-CRP, whereas improvements in PI and GI were observed in the control group at 1, 3 and 6 months of follow-up. However, the PPD, CAL, metabolic parameters and hs-CRP increased in the control group at 3 and 6 months of follow-up, but the differences were not significant. CONCLUSIONS: NSPT improves the periodontal status of smokers with T2D, has a favorable effect on glycemic control and reduces pro-inflammatory mediators, which may limit complications due to T2D in these patients.

Morphological features of smile attractiveness and related factors influence perception and gingival aesthetic parameters.

INTRODUCTION: In addition to understanding the basic standards of a smile and patient's opinion, dentists should take into account smile aesthetics, an essential factor for optimal outcomes. This study aimed to evaluate the factors that affect the perception of an aesthetic smile and determine its morphological characteristics and measure the gingival aesthetic parameters. METHODS: In all, 200 Vietnamese aged 18-35 years were recruited to have their spontaneous smiles captured. These smile images were assessed by 50 laypersons and 50 dentists using a visual analogue scale measurement. The images were analysed to evaluate perceptions of evaluators, determine smile attractiveness, and measure gingival aesthetics. RESULTS: The difference in the judgements of laypersons and dentists, males and females, and laypersons aged 18-25 and 26-35 years were nonsignificant. High or average anterior smile line, parallel smile arc, upward upper lip curvature, second premolars as the posterior-most teeth displayed, smile index of 5.23-5.63, and dynamic smile symmetry of 1 were scored highly on smile attractiveness. The following maxillary gingival aesthetic parameters were preferred: gingival zenith (GZ) of the canine 0.72-0.75 mm apical to the GZ of the central incisor; GZ of the lateral incisor 0.66 mm coronal to the gingival line; gingival line angle of ∼87°; for the central incisor, lateral incisor, and canines, distance from the GZ to the long axis of 1, 0.4, and 0 mm, respectively; interdental papilla height of 4.25, 3.60, and 3.85 mm, respectively; ratio of the distance from the GZ and the interdental papilla tip to the incisal edge of ∼1.74-1.77 mm. CONCLUSIONS: Factors including profession, gender, and age of evaluators had almost no impact on their perception of aesthetics. Smile attractiveness characteristics and gingival aesthetic parameters have clinical applicability for patient care.

INTRABONY DEFECT TREATMENT WITH PLATELET-RICH FIBRIN, GUIDED TISSUE REGENERATION AND OPEN-FLAP DEBRIDEMENT: A RANDOMIZED CONTROLLED TRIAL.

OBJECTIVE: To comparatively assess the outcomes of the treatment of periodontal intrabony defects with platelet-rich fibrin (PRF) combined with open-flap debridement (OFD), guided tissue regeneration (GTR) or OFD alone based on clinical, radiographic, and wound healing parameters for 12 months of follow-up. MATERIALS AND METHODS: Ninety intrabony defects were randomly divided into 3 different groups and treated as group 1 (PRF + OFD), group 2 (GTR), or group 3 (OFD alone). Clinical parameters, including the plaque index, gingival index, bleeding on probing, probing depth (PD), clinical attachment loss (CAL), and tooth mobility were assessed at 3, 6, and 12 months. Additionally, the wound healing index was assessed at 7 and 14 days postsurgery. Radiographic parameters, including bony defect fill and alveolar crestal resorption, were measured at 6 and 12 months postsurgery and calculated using image analysis software. RESULTS: Intragroup comparisons showed consistently significant improvements in all the clinical and radiographic parameters in the 3 groups at 12 months postsurgery. Compared to baseline, in group 1, the PD decreased to 2.37 ±â€¯0.56; 3.30 ±â€¯0.84; 4.80 ±â€¯0.71 mm, and CAL decreased to 2.23 ±â€¯0.90; 3.33 ±â€¯0.71; 5.00 ±â€¯0.46 mm; in group 2, the PD decreased to 2.30 ±â€¯0.60; 3.23 ±â€¯0.86; 4.63 ±â€¯0.67 mm, and CAL decreased to 2.00 ±â€¯0.98; 3.20 ±â€¯0.71; 4.53 ±â€¯0.57 mm); and in group 3, the PD decreased to 1.87 ±â€¯0.68; 2.57 ±â€¯1.36; 3.37 ±â€¯1.00 mm, and CAL decreased to 1.60 ±â€¯0.93; 2.23 ±â€¯1.22; 3.37 ±â€¯1.22 mm at 3, 6, and 12 months postsurgery, respectively. The bone fill percentages in group 1 (26.45 ±â€¯16.47 and 45.25 ±â€¯5.20%), group 2 (22.20 ±â€¯15.76 and 42.15 ±â€¯6.39%) and group 3 (10.21 ±â€¯6.47 and 23.13 ±â€¯6.98%) were observed at 6 and 12 months postsurgery. The alveolar crestal resorption levels were -1.07 ±â€¯0.52 and -1.70 ±â€¯0.60 mm in group 1, -1.03 ±â€¯0.72 and -1.47 ±â€¯0.73 mm in group 2 and 0.37 ±â€¯0.62 and 0.43 ±â€¯0.73 mm in group 3, respectively. The percentages of sites with a WHI score of 1 were 93% and 100% in group 1, 81%, and 94% in group 2 and 45% and 71% in group 3 at 7 and 14 days postsurgery, respectively. CONCLUSIONS: Compared to GTR, PRF yielded comparable treatment outcomes and periodontal tissue healing in terms of improvements in clinical and radiographic parameters. Compared to OFD alone, PRF also significantly improved these parameters in the treatment of intrabony defects.

Comparison of Subgingival Irrigation Effect of Boric Acid 0.5% and Povidone-Iodine 0.1% on Chronic Periodontitis Treatment.

PURPOSE: To comparatively evaluate the effect of a 5% boric acid (BA) irrigant on periodontal condition, bacterial level and oral neutrophil numbers with a 1% povidone iodine (PVP-I) irrigant as an adjunct to scaling and root planing (SRP) in chronic periodontitis (CP) treatment. MATERIALS AND METHODS: A single-masked, randomised clinical trial with 36 CP patients was conducted at the Faculty of Odonto-Stomatology, University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam. Subjects were randomly divided into two treatment groups: 1) SRP plus PVP-I 0.1% irrigant and 2) SRP plus BA 0.5% irrigant. Clinical measurements, including the plaque index (PI), gingival index (GI), bleeding on probing (BOP), probing depth (PD), clinical attachment level (CAL), bacterial level in subgingival plaque (BANA test) and the quantification of oral neutrophils were evaluated at baseline, 4, 6 and 8 weeks after treatment (T0, T4, T6 and T8). RESULTS: Whole-mouth (PI, GI, BOP, PD, CAL and PD) parameters, bacterial level in subgingival plaque and number of oral neutrophils decreased statistically significantly after treatment compared to baseline in both groups (p < 0.01). Between the two groups, whole-mouth PI, GI, BOP, PD and CAL reduction in the BA 0.5% group were higher than those in the PVP-I 0.1% group, but statistical significance was found only for GI and BOP after treatment (p < 0.05). The PD and CAL reductions for moderately deep pockets (PD ≥ 5 mm and < 7 mm) were significantly greater in group 2 compared to group 1 after treatment compared to baseline (p < 0.01). This difference was not found for deep pockets (PD ≥ 7 mm). CONCLUSION: The results of this study suggest that BA 0.5% could be an alternative to PVP-I 0.1%, and might be more favourable because it provided superior results regarding whole-mouth BOP, GI as well as PD and CAL reduction for moderately deep pockets after CP treatment.

In vitro characteristics of human periodontal ligament stem cells incubated with boric acid.

OBJECTIVES: To evaluate the influence of boric acid (BA) on various aspects of the human periodontal ligament stem cells (hPDLSCs), including proliferation, migration, and adhesion. METHODS: BA was prepared at increasing concentrations of 0.5%, 0.75%, 1%, 1.5%, 3%, and 6%, and an [3-(4,5-dimethylthiazol-2-YL)-2,5-diphenyl] (MTT) assay was used to determine the non-toxic concentrations of BA on hPDLSCs. The appropriate concentrations of BA were applied in subsequent experiments to evaluate its effect on three particular aspects of hPDLSCs: proliferation, migration, and adhesion. The proliferation of hPDLSCs was determined with a hemocytometer. The migration of hPDLSCs was evaluated using a scratch assay, followed by analysis of the recorded digital images using the Image-Analysis J 1.51j8 software. The adhesion of hPDLSCs to sterilized root surfaces that retained or lacked a cementum was assessed using the MTT assay. RESULTS: Non-toxic concentrations of BA against hPDLSCs were 0.5% and 0.75%, allowing a relative growth rate of greater than 75%. Treated hPDLSCs proliferated and migrated uneventfully, with no significant differences to the control group. hPDLSCs adhered to both types of the prepared root surfaces; however, those lacking a cementum allowed more effective adhesion than those retaining it. Qualitatively, the adhesion ability of hPDLSCs was better in the 0.5% rather than the 0.75% BA-treated or control groups. CONCLUSIONS: BA at concentrations of 0.5% and 0.75% did not impede the proliferation, migration, or adhesion ability of hPDLSCs to root surfaces, indicating that this sterilizing agent could be advanced to clinical trials.

Antimicrobial Effect of Platelet-Rich Plasma against Porphyromonas gingivalis.

AIM: The aim of our study was to evaluate whether there was a difference in antimicrobial effect between the PRP of healthy volunteers and that of patients with chronic periodontitis against P. gingivalis, which was fresh cultured from subgingival plaque. METHODS: Subgingival plaque of patients with moderate and severe chronic periodontitis was collected to isolate P. gingivalis. The PRP of four individuals with healthy periodontium and four patients with moderate and severe periodontitis were collected with a specific kit using a two-centrifuge procedure, and then, the antibacterial properties against P. gingivalis were tested, through their minimum inhibitory concentration (MIC), adhesion resistance assay, and biofilm susceptibility assay. RESULTS: P. gingivalis was successfully isolated from the subgingival plaque of the 21st patient. The round, smooth, and black colony appeared in the agar disk after 7-10 days of incubation under anaerobic conditions. Bacterial identification was performed by MALDI-TOF and confirmed by PCR. All PRP samples tested showed the ability to inhibit P. gingivalis growth. The MIC value (expressed as fraction of PRP) was 1/2, and PRP prevented P. gingivalis attachment on the disk surface. However, PRP did not have a strong effect on the suppression of P. gingivalis biofilm. CONCLUSION: PRP of individuals with healthy periodontium and chronic periodontitis patients showed antibacterial properties against P. gingivalis. This material can become an adjunct to periodontal treatment.

In Vitro Evaluation of Proliferation and Migration Behaviour of Human Bone Marrow-Derived Mesenchymal Stem Cells in Presence of Platelet-Rich Plasma.

OBJECTIVE: To access the effects of platelet-rich plasma (PRP) on the behaviour of human bone marrow-derived mesenchymal stem cells (hBMSCs), including proliferation and migration. METHODS: PRP was diluted with DMEM/F12, resulting in concentrations of 1%, 2%, and 5%. The proliferation of hBMSCs was examined by 2 methods: cell-number counting with the haemocytometer method and the colony-forming unit-fibroblast (CFU-F) assay. Cell migration was evaluated using the scratch wound healing (SWH) assay; after that, the recorded digital images were analysed by the Image-Analysis J 1.51j8 software to compare the cell-free areas between groups after 0, 24, and 48 hours. RESULTS: hBMSCs cultured in DMEM/F12 at PRP concentrations of 1%, 2%, and 5% were all able to proliferate and migrate. In the 5% PRP group, hBMSCs proliferated greatly with a significantly higher cell number than reported for all other groups on days 5, 7, and 9. CFU-Fs were observed in all groups, except for the negative control group. The SWH assay demonstrated that hBMSCs cultured in 2% and 5% PRP almost filled the artificial wound scratch and significantly migrated more than those of all other groups at both 24 h and 48 h. CONCLUSION: This study indicated that, due to the significant enhancement of cell proliferation and migration, 5% PRP might be the optimal concentration that should be used to promote the potential of hBMSCs in wound healing.

An Update on Anti-CD137 Antibodies in Immunotherapies for Cancer.

The selective expression of CD137 on cells of the immune system (e.g., T and DC cells) and oncogenic cells in several types of cancer leads this molecule to be an attractive target to discover cancer immunotherapy. Therefore, specific antibodies against CD137 are being studied and developed aiming to activate and enhance anti-cancer immune responses as well as suppress oncogenic cells. Accumulating evidence suggests that anti-CD137 antibodies can be used separately to prevent tumor in some cases, while in other cases, these antibodies need to be co-administered with other antibodies or drugs/vaccines/regents for a better performance. Thus, in this work, we aim to update and discuss current knowledge about anti-cancer effects of anti-CD137 antibodies as mono- and combined-immunotherapies.

Periodontal Status of the Adjacent Second Molar after Impacted Mandibular Third Molar Surgical Extraction.

OBJECTIVE: The purpose of this study was to evaluate the change in periodontal status of the adjacent second molar of the impacted mandibular third molar after surgical extraction and its association with the third molar condition in the presurgical stages, including position, eruption level, and local complications. MATERIALS AND METHODS: The study was based on a 6-month follow-up of 38 patients (19 males and 19 females; Mean age: 21.89 ± 2.74) recruited consecutively after surgical extraction of an impacted lower third molar. The third molar's presurgical position, eruption level, and local complications were examined. Periodontal status, including Plaque Index (PI), Gingival Index (GI), and gingival bleeding on probing (BOP), of the teeth in the adjacent sextant was clinically evaluated. The pocket depth (PD) and the distance between the epithelial attachment and the adjacent second molar's occlusal surface were clinically measured; and the distance between the alveolar bone crest and cementoenamel junction (AC-CEJ) of the adjacent second molar was evaluated by the periapical film. All measures were recorded at the time of surgery and 1, 3, and 6 months after surgery. RESULTS: The values of PI, GI, BOP, PD, and EA-OS were significantly reduced after 1, 3, and 6 months compared to baseline data. The AC-CEJ was decreased after 1 month but significantly increased after 3 and 6 months. Presurgical local complications of the impacted third molar mostly were significantly associated with the periodontal status of the adjacent sextant. CONCLUSION: There was a significant improvement of periodontal conditions of the second molar and adjacent sextant after impacted third molar surgery.

Factors related to dental caries in 10-year-old Vietnamese schoolchildren.

BACKGROUND: In Vietnam, research is required to provide a clearer understanding of the factors associated with dental caries. This will allow paediatric dentists and physicians to provide appropriate care to children. AIM: To determine the factors related to dental caries and to evaluate the association between dental caries and nutritional status in 10-year-old schoolchildren. METHOD: In 2015, 1,079 10-year-old children were randomly selected from 16 public schools in Ho Chi Minh City. Information on self-reported oral health, dental habits, eating, drinking and physical activity behaviours was obtained via a questionnaire. Anthropometric measurements (height and weight) were also taken. Body mass index (BMI) was calculated, and nutritional classification of children was conducted using the BMI-for-age percentile. Dental plaque and dental caries (decayed and filled teeth) were recorded for both primary and permanent teeth. RESULTS: Dental caries was experienced on primary and permanent teeth by 60.7% and 39.6%, respectively, of subjects. The factors significantly related to primary tooth decay were school location [odds ratio (OR) = 1.78], self-reported dental caries (OR = 1.36), frequency of tooth self-checking (OR = 1.32), toothbrushing habits (OR = 1.31) and nutritional status (OR = 1.77); however, the factors associated with permanent tooth decay were school location (OR = 2.66), self-reported dental caries (OR = 1.34), frequency of tooth self-checking (OR = 1.37), frequency of dental check-ups (OR = 1.35), toothbrushing habits (OR = 1.31) and nutritional status (OR = 1.39 and 1.74) (P < 0.05). CONCLUSION: Dental caries was associated with school location, dental-related behaviours and nutritional status in 10-year-old schoolchildren in Ho Chi Minh City, Vietnam.

Efficacy of chlorine dioxide mouthwash in reducing oral malodor: A 2-week randomized, double-blind, crossover study.

The aim of this clinical trial was to assess the inhibitory effects of a mouthwash containing 0.1% ClO2 used for 2 weeks on oral malodor, periodontal and salivary parameters, tongue coating, and Gram-negative and Gram-positive bacteria in saliva. Thirty-nine subjects with oral malodor were randomly assigned into two groups. In the first stage, one group was instructed to rinse with the experimental mouthwash (containing 0.1% ClO2), whereas the other group was instructed to rinse with the control mouthwash (sodium chloride 0.9%) for 2 weeks. After 4 weeks of washing out, in the second stage, each group then used the other mouthwash for 2 weeks. Oral malodor, periodontal status, tongue coating, salivary pH and flow rate, and the amounts of the salivary bacteria Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis, Solobacterium moorei, Streptococcus salivarius, Treponema denticola, and Tannerella forsythia were evaluated at baseline and after 2 weeks of mouthwash use. After 12 hr and after 2 weeks, organoleptic scores and the levels of H2S and CH3SH were significantly lower in the experimental group compared with those in the control group. After 2 weeks, the experimental mouthwash appeared significantly effective in reducing plaque index, tongue-coating score, and the amounts of F. nucleatum, S. moorei, T. denticola, and T. forsythia in the whole saliva, compared with those at baseline. Mouthwash containing 0.1% ClO2 is effective in reducing oral malodor, dental plaque, tongue-coating accumulation, and the amounts of F. nucleatum, S. moorei, T. denticola, and T. forsythia in saliva.

Periodontal status of anterior teeth following clinical crown lengthening by minimally traumatic controlled surgical extrusion.

BACKGROUND/AIMS: The number of fractured anterior teeth following trauma has been increasing while not every patient is able to afford a dental implant instead of maintaining the injured tooth. Thus, a tooth conservation solution is required to place an aesthetic and functional restoration without biologic width violation. The aim of this study was to evaluate the effectiveness of minimally traumatic controlled surgical extrusion in fractured anterior teeth crown lengthening by assessing the periodontal status through clinical examination and radiographs. METHODS: This longitudinal observational study investigated a group of 18 patients (six males and 12 females) at the Department of Periodontology, National Hospital of Odonto-stomatology, Ho Chi Minh City, Vietnam. Following pre-surgery procedures and examination, minimally traumatic controlled surgical extrusion was carried out using a periotome. Patients were examined at four follow-up appointments after 1 week, 1, 3 and 6 months to record the following experimental variables: periodontal parameters including the gingival index (GI), pocket depth (PD), bleeding on probing (BOP), mobility, marginal gingiva position, alveolar ridge resorption, periapical osteogenesis, tooth resorption and ankylosis. RESULTS: All periodontal parameters were significantly decreased at 3 and 6 months post-procedure (P < 0.001). Tooth mobility decreased gradually following surgery, and at 6 months, all teeth became normal at level 0. Periapical osteogenesis changes were significantly increased at 1, 3 and 6 months in comparison with pre-surgery (P < 0.001). Marginal gingiva position and alveolar ridge resorption were not significantly different between pre-surgery and 1, 3 and 6 months post-surgery. No cases of root resorption or ankylosis were observed at 6 months post-surgery. CONCLUSION: A minimally traumatic controlled surgical extrusion technique for clinical crown lengthening yielded highly successful results in both aesthetic and functional aspects, and no cases had unfavourable outcomes during the 6-month follow-up period.

Effects of platelet-rich plasma on human gingival fibroblast proliferation and migration in vitro.

OBJECTIVE: This study evaluated the influence of platelet-rich plasma (PRP) on the behaviour of human gingival fibroblasts (hGFs), including fibroblast proliferation, migration and colony formation. METHODS: PRP was obtained from the human peripheral blood of a healthy volunteer and then was diluted into platelet concentrations of 1%, 2% and 5%. The proliferation of hGFs was determined by two methods: (1) Cell-number counting with a haemocytometer method at days 1, 3, 5 and 7; (2) Colony-forming unit-fibroblast (CFU-F) assay at 2 weeks. The migration of hGFs was evaluated with scratch assay, then recorded digital images were analysed by Image-Analysis J 1.51j8 software to compare the remaining artificial wound areas between PRP groups at 0, 24 and 48 hours. RESULTS: All hGFs that were cultivated in media with 1%, 2% and 5% PRP showed their ability to proliferate and migrate. Cell numbers incubated with 1% PRP increased significantly during the first three days and peaked at day 5, tending to be similar to their proliferation in complete medium. With concentrations of 2% and 5% PRP, hGFs outgrew and peaked at day 3, which was faster than with those in medium with 1% PRP. Especially, hGFs in the group 5% PRP proliferated with higher cell numbers than those in the other remaining groups at day 3. The hGF colony number that was formed in the group 5% PRP was significantly higher than those in the groups 1% and 2% PRP. Scratch assay showed hGFs in the groups 2% and 5% PRP almost filled the artificial wound and migrated more effectively than in the group 1% PRP at 24 hours, which was significant. CONCLUSION: In this study, perhaps the medium with 5% PRP is the dominant option, promoting the abilities of hGFs to heal wounds, because of its fast and effective impact on cell proliferation, colony formation and migration.

The interaction among obesity, Type 2 diabetes mellitus, and periodontitis in Vietnamese patients.

To examine the relationship between obesity, Type 2 diabetes, and periodontal disease in Vietnamese patients. The sample included 712 patients aged 18 years or older who first visited the Institute of Traditional Medicine, Ho Chi Minh City. All participants completed a questionnaire and underwent anthropometric index measurements for obesity (height, weight, waist, and hip circumferences) and had their body fat percentage measured. A full periodontal examination was performed and a fasting glycemic level was determined. Occurrence and risk of periodontal outcomes were compared across 3 different measurements of obesity (body mass index, waist-hip ratio, and body fat percentage). The prevalence of periodontitis in obese group (37.0%, 36.4%, and 24.6% by body mass index, waist-hip ratio, and body fat percentage, respectively) or Type 2 diabetic group (50.7%) was significantly higher than those without these conditions (p < .05). Subjects with obesity or Type 2 diabetes had significantly greater pocket depth and clinical attachment loss than those who are not obese or diabetic (p < .001). Multivariate logistic regression, adjusted for confounding variables, showed that the likelihood (odds ratio, OR) for periodontitis was highest in the obese and Type 2 diabetic group (OR = 4.24, CI [2.29, 7.86]; OR = 4.06, CI [2.24, 7.36]; and OR = 5.44, CI [2.94, 10.03]), followed by the obese and non-Type 2 diabetic group (OR = 2.28, CI [1.05, 4.95]; OR = 2.02, CI [1.34, 3.56]), and then the nonobese and Type 2 diabetic group (OR = 2.20, CI [1.21, 3.98]; OR = 1.99, CI [0.93, 4.24] and OR = 5.22, CI [2.76, 9.84]) when obesity was defined by body mass index, waist-hip ratio, and body fat percentage, respectively, (p < .05). There was a significant association between obesity, Type 2 diabetes, or those with both systemic conditions and periodontitis in Vietnamese patients.

Dental and periodontal problems of elderly people in Vietnamese nursing homes.

OBJECTIVE: To determine the oral health status and treatment needs of elderly residents in Vietnamese nursing homes. METHODS: This study was conducted among 360 men and 431 women with a mean age of 72.9 ± 9.1 years in three nursing homes in Ho Chi Minh City. Demographic information on education, duration of stay, chronic diseases, tooth brushing and tobacco use were obtained from medical records and a questionnaire. The oral health of participants was clinically assessed by a dental examiner using WHO criteria. Oral health status included estimates of coronal and root caries, periodontal disease, denture status and dental treatment needs. RESULTS: Nearly all (90.1%) of the participants had natural teeth. The mean numbers of teeth with coronal or root decay were 5.8 ± 4.0 and 6.0 ± 4.2, respectively, and almost everyone required at least one restoration for coronal or root caries. Most of the participants with natural teeth had bleeding gingiva on probing, 26.2% had deep periodontal pockets, 96.5% needed oral hygiene improvement plus scaling and 20.3% required complex periodontal treatment. None of the edentulous participants had dentures, and 86.6% required new or repaired dentures. CONCLUSION: The prevalence of untreated oral diseases is very high and the need for dental care extensive among the residents of government-administered long-term care facilities in Ho Chi Minh City.

Effects of platelet-rich plasma on human gingival fibroblast proliferation and migration in vitro

Abstract Objective This study evaluated the influence of platelet-rich plasma (PRP) on the behaviour of human gingival fibroblasts (hGFs), including fibroblast proliferation, migration and colony formation. Methods PRP was obtained from the human peripheral blood of a healthy volunteer and then was diluted into platelet concentrations of 1%, 2% and 5%. The proliferation of hGFs was determined by two methods: (1) Cell-number counting with a haemocytometer method at days 1, 3, 5 and 7; (2) Colony-forming unit-fibroblast (CFU-F) assay at 2 weeks. The migration of hGFs was evaluated with scratch assay, then recorded digital images were analysed by Image-Analysis J 1.51j8 software to compare the remaining artificial wound areas between PRP groups at 0, 24 and 48 hours. Results All hGFs that were cultivated in media with 1%, 2% and 5% PRP showed their ability to proliferate and migrate. Cell numbers incubated with 1% PRP increased significantly during the first three days and peaked at day 5, tending to be similar to their proliferation in complete medium. With concentrations of 2% and 5% PRP, hGFs outgrew and peaked at day 3, which was faster than with those in medium with 1% PRP. Especially, hGFs in the group 5% PRP proliferated with higher cell numbers than those in the other remaining groups at day 3. The hGF colony number that was formed in the group 5% PRP was significantly higher than those in the groups 1% and 2% PRP. Scratch assay showed hGFs in the groups 2% and 5% PRP almost filled the artificial wound and migrated more effectively than in the group 1% PRP at 24 hours, which was significant. Conclusion In this study, perhaps the medium with 5% PRP is the dominant option, promoting the abilities of hGFs to heal wounds, because of its fast and effective impact on cell proliferation, colony formation and migration.

Validation of the salivary urea and creatinine tests as screening methods of chronic kidney disease in Vietnamese patients.

OBJECTIVES: The aims of this case control study were to correlate the serum and salivary urea as well as creatinine levels; and to evaluate salivary urea and creatinine as noninvasive alternatives to serum for creatinine estimation in chronic kidney disease (CKD) patients. METHODS: Blood and saliva samples were collected from 112 CKD patients and 108 subjects without CKD for quantitative analysis of urea and creatinine. Spearman's correlation coefficients between salivary and serum urea as well as creatinine were obtained. Receiver operating characteristic analysis was done to assess the diagnostic tests of salivary urea and creatinine. Cut-off values were determined based on the best trade-off between the sensitivity and specificity for both salivary urea and creatinine. RESULTS: Salivary urea and creatinine concentrations were significantly higher in CKD patients than those in control subjects; and increased by the stages of the severity of the disease. The positive correlation was significantly found between salivary and serum creatinine (r = 0.90) and between salivary and serum urea (r = 0.73). Area under the curve for salivary urea was 0.76 and a cut-off value of 14.25 mmol/L gave a sensitivity of 82.9% and specificity of 57.8%. Area under the curve for salivary creatinine was 0.92 and a cut-off value of 0.24 mg/dL gave a sensitivity of 86.5% and specificity of 87.2%. CONCLUSIONS: Both salivary urea and creatinine have a high capacity for serum creatinine estimation. Salivary urea and creatinine tests can be used as low-cost, easily accessible and noninvasive tools for screening, diagnosing, monitoring treatment outcomes and ascertaining prognosis of chronic kidney disease.