Global DNA methylation status in laryngeal cancer.

BACKGROUND: The purpose of our study was to evaluate if DNA methylation level in leukocytes may be used as a surrogate marker of genome methylation status in laryngeal cancer tissues. METHODS: We evaluated global DNA methylation using an ultra performance liquid chromatography (UPLC)-based method to assess the total content of 5-methylcytosine (5 mC). RESULTS: The study was performed on DNA isolated from cancer tissues, adjacent normal tissues, and peripheral blood leukocytes in a group of 72 patients with laryngeal cancer. DNA hypomethylation was found in tumor tissue (56%) and normal tissue (49%). There was a significant correlation between the levels of 5 mC in these 2 types of tissue. There was no significant DNA hypomethylation in blood. A negative correlation between tumor grade and blood levels of 5 mC was found. CONCLUSION: The level of leukocyte DNA methylation measured using total 5 mC content cannot be used as a surrogate marker for genome methylation status in laryngeal cancer tissues.

[Salivary tumors in the material of the Otolaryngology Clinical Ward of 4th Military Clinical Hospital with the Polyclinic in Wroclaw in the years 1992- 2008]. / Guzy slinianek w materiale Klinicznego Oddzialu Otolaryngologicznego 4 Wojskowego Szpitala Klinicznego z Poliklinika we Wroclawiu w latach 1992-2008.

AIM: The aim of the study is the analyses of salivary gland tumors on 193 patients who underwent surgery at the Otolaryngology Clinical Ward of 4th Military Clinical Hospital with the Polyclinic in Wroclaw in the years 1992-2008. MATERIAL AND METHODS: In the group of patients with salivary gland tumors, which were analysed, there were 106 woman (54.9%) and 87 men (45.1%). The following data: age, sex, time between appearing first symptoms and operation, objective and subjective symptom, the kind of the executed operation and the result of histopathological examination were taken into account in this study. RESULTS AND CONCLUSIONS: Tumors located in 48.7% in parotid gland, 49.7% in submandibular salivary gland and 1.6% in hypoglossal salivary gland. Tumor--like lesions constituted 56%, adenoma 38.9% and malignat tumors 4.7% of all glands tumors. Tumors salivary glands both benign and malignat occur at women about the average age of 50.9 more often. Pleomorphic adenoma was the most common neoplasm. Tumors located in parotid salivary glands are often on the left side.

[Endoscopic surgical treatment of isolated inverted papilloma of the sphenoid sinus complicated by cerebrospinal fluid (CSF) rhinorrhea--case report]. / Endoskopowa operacja izolowanego brodawczaka odwróconego zatoki klinowej powiklana plynotokiem nosowym--opis przypadku.

This report presents a case of isolated inverted papilloma of the sphenoid sinus in 48-year old patients diagnosed and treated in ENT Departament of Military Hospital in Wroclaw. Post operative course complicated by cerebrospinal fluid (CSF) rhinorrhea. We used Surgicel, lyophylized dura and TachoComb with Tissucol to close dural fistula. Using of this in reconstructive surgery to treat post operative CSF leak was effective. In last CT three years after operation, we observe the bone decline in sphenoid sinus and recurrence of the leasion in sphenoid sinus.