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STAR Protoc
; 4(4): 102632, 2023 Dec 15.
Artigo
em Inglês
| MEDLINE
| ID: mdl-37838948
RESUMO
Cellular redox state determinants are traditionally studied using fluorescent microscopy and immunoblot analysis; however, no procedure has been developed for simultaneous measurement in various immune cell subsets. Here, we present a flow cytometry assay for measuring antioxidant defense systems and reactive oxygen species simultaneously in T, B, and natural killer lymphocytes. We describe steps for preparing and treating peripheral blood mononuclear cells, surface and dye staining, cell fixation/permeabilization, and intracellular staining. We then detail machine standardization, acquisition, and analysis.
Assuntos
Leucócitos Mononucleares , Humanos , Citometria de Fluxo/métodos , Espécies Reativas de Oxigênio , Immunoblotting , Oxirredução
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