RESUMO
Polymorphism of PON-1 gene in 192 position of amino acid sequence of enzyme paraoxonase 1 was studied. For this research we have used the blood samples of 96 patients with T2DM and 123 healthy habitants of Kharkiv. Frequencies of alleles for the patients (pQ = 0,65, pR = 0,35) and the healthies (PQ = 0,70 and pR = 0,30) did not differentiate meaningfully. Distribution of the genotypes for healthy people does not correspond to Hardy-Weinberg equilibrium, the patients of T2DM have surplus of both homozygotes and shortage of heterozygotes. The risk of illness of T2DM for QQ homozygotes is 1,46-times higher and for QR heterozygotes is two times lower than on the average in the population (2%). The risk of illness of T2DM for RR homozygotes 1,86-times exceeds a middle population risk but is not significant.
Assuntos
Arildialquilfosfatase/genética , Diabetes Mellitus Tipo 2/genética , Polimorfismo de Nucleotídeo Único , Estudos de Casos e Controles , DNA/genética , Diabetes Mellitus Tipo 2/enzimologia , Eletroforese em Gel de Ágar , Feminino , Frequência do Gene , Predisposição Genética para Doença , Homozigoto , Humanos , Masculino , RiscoRESUMO
Polymorphism of adiponectine (APM1) gene in +276G > T position was studied. For this research we used blood samples of 103 donors (men/women: 65/38; 70 Ukrainian, 33 Russian) - habitants of Kharkiv. Frequencies of T and G alleles were pT = 0.55 and pG = 0.45 in a general group. They did not meaningfully differentiate either for men and women or for Russians and Ukrainians. Distribution of genotypes did not correspond to Hardy-Weinberg equilibrium: share of heterozygotes was 1.55 times higher than selectively-neutral value, share of TT homozygote is 0.55, and GG homozygote is 0.33 of equilibrium value.
Assuntos
Doadores de Sangue , Polimorfismo de Nucleotídeo Único , Adiponectina/genética , Adulto , Doadores de Sangue/estatística & dados numéricos , Cidades , DNA/genética , Feminino , Frequência do Gene , Testes Genéticos , Heterozigoto , Humanos , Leucócitos/metabolismo , Masculino , Pessoa de Meia-Idade , Federação Russa/etnologia , Ucrânia/epidemiologia , Adulto JovemRESUMO
Polymorphism of PON-1 gene in 192 position of amino acid sequence of enzyme paraoxonase 1 related to the synthesis of allozymes R or Q was studied. For this research we used blood samples of 109 donors (men/women: 78/31; 84 Ukrainian, 25 Russian)--habitants of Kharkiv. Frequency of Q and R alleles were pQ = 0.67, pR = 0.33. Distinctions of allele frequencies among Ukrainians and Russians and also among men and women were not significant. Distributing of genotype frequencies in the Slavonic population of Kharkiv corresponds to panmictic population.