Stability-indicating reversed-phase-HPLC method development and validation for sacubitril/valsartan complex in the presence of impurities and degradation products: Robustness by quality-by-design approach.

According to current regulatory guidelines, a stability-indicating method has been developed to determine the impurities in sacubitril (SCB) and valsartan (VLS) tablet dosage forms and perform robustness studies using the design of experiments approach. The present study was initiated to understand quality target product profile, analytical target profile, and risk assessment for method variables that affect the method response. A reversed-phase-HPLC system was equipped with a Phenomenex Gemini-NX C18 column (150 × 4.6 mm, 3 µm) and a photo diode array detector. A gradient mobile phase was used in this research work. The detection was performed at 254 nm; the flow rate was 1.5 mL/min, and the column temperature was maintained at 30°C. The proposed method was validated per the International Council for Harmonisation Q2 (R1) guidelines. The coefficient of correlation was >0.999 for all impurities. The limits of detection and quantification were evaluated for SCB, VLS, and all impurities. The precision and accuracy were obtained for SCB, VLS, and their related impurities. Intra- and inter-day relative standard deviation values were less than 10.0%, and the recoveries of impurities varied between 90.0 and 115.0%. Based on the validation results, the proposed DoE method can estimate SCB and VLS impurities in the finished dosage form.

Novel reversed-phase HPLC method for simultaneous determination of ethynodiol diacetate (EDA)/ethinyl estradiol (EE) in pharmaceutical dosage form.

Ethynodiol diacetate (EDA) and ethinyl estradiol (EE) tablets are indicated to prevent pregnancy in women who use oral contraceptives as a contraception method. EDA and EE were separated by reversed-phase HPLC using Agilent ZORBAX SB-Phenyl column, 4.6 mm × 15 cm, 5 µm, using a gradient mixture of acetonitrile and Milli-Q water as mobile phase. The linearity and recovery were found in the range of 0.025-0.25 mg/mL and 0.05-0.18 mg/mL for EDA and 0.001-0.01 mg/mL and 0.002-0.007 mg/mL for EE, respectively. The method is validated according to the regulatory guidelines concerning system suitability, specificity, repeatability, recovery, linearity, robustness, and stability of the sample solution.

Efficacy of biochar in removal of organic pesticide, Bentazone from watershed systems.

Bentazone is one of the toxic insecticides used to control forest tent caterpillar moths, boll weevils, gypsy moths, and other types of moths in various field crops. We report the efficacy of biochar prepared from the Azardirachta Indica waste biomass as adsorbent for removal of Bentazone. Biochar material was prepared by pyrolysis process under limited oxygen conditions. Biochar material was characterized by proximate and ultimate analysis, SEM analysis, FTIR analysis and TG/DTA analyses. The Bentazone adsorption capacity by biochar from aqueous solutions was assessed. Effect of time, adsorbent dosage, insecticide concentration and pH on the adsorption characteristics of the biochar were evaluated. Adsorption parameters were obtained at equilibrium contact time of 150 min, with biochar dosage of 0.5 g at pH 8. From the optimization studies, desirability of 0.952 was obtained with response (adsorption uptake) of 79.40 mg/g, for initial concentration of insecticide (50 mg/L), adsorbent dosage (0.448 g), time 30.0 min and pH 2. The adsorption isotherm data for the removal of Bentazone fitted well with the Freundlich isotherm. This study indicates that the biochar produced from the bark of Azardirachta Indica biomass could be employed as a potential adsorbent for removal of synthetic organic pollutants from the water streams.