Effects of fermented rapeseed meal on performance, intestinal morphology, the viscosity of intestinal content, phosphorus availability, and egg quality of laying hens.

Fermented rapeseed meal has the potential to partial replace soybean meal in feed mixtures for poultry without a negative impact on the health condition and performance of birds. This is due to the fact that the fermentation process can reduce the amount of antinutritional factors, improve the use of nutrients and impart probiotic properties to rapeseed meal. Therefore, this study was undertaken to investigate the effect of fermented rapeseed meal on the performance, egg quality, intestinal morphometry, the viscosity of intestinal content and total phosphorus availability. A total of 108 Lohmann Brown laying hens at 26 wk of age were used in the 90-day study. All hens were randomly divided into 3 treatment groups, with 12 replicates (cages) each, as follows: control group received no rapeseed meal, the URSM group received 3% unfermented rapeseed meal and the FRSM group received 3% fermented rapeseed meal. In the case of performance, egg traits, sensory evaluation of eggs, the viscosity of intestinal content and the availability of total phosphorus, if the distribution was normal, a 1-way analysis of variance was performed. If the distribution was not normal, the Kruskal-Wallis test was performed. In the case of histomorphometric evaluation of the intestine, if the distribution was normal, the Student t test for independent samples was performed. If not, a Mann-Whitney U test was performed. The performed analyses showed that the supplementation of fermented rapeseed meal had no negative effect on the performance of birds and the quality of eggs. Fermented rapeseed meal was also associated with improved histomorphometric parameters of the small intestine compared to the group receiving unfermented rapeseed meal in the feed. Laying hens from FRSM group were characterized by significantly lower viscosity of intestinal content (P < 0.05) compared to URSM group. Phosphorus in FRSM group was significantly more available to the birds (P < 0.05) compared to URSM group. These results suggest that supplementation with fermented rapeseed meal may be beneficial, especially in times of unstable prices of soybean meal and problems with its availability.

Influence of solid state fermentation with Bacillus subtilis 67 strain on the nutritional value of rapeseed meal and its effects on performance and meat quality of broiler chickens.

The study aimed to evaluate the influence of solid-state fermentation on the nutritional value and enzymatic activity of rapeseed meal and its effects on the performance of broiler chickens and meat quality, including physicochemical properties (proximate analysis, pH, water holding capacity), antioxidant capabilities, dipeptide composition of the meat and sensory traits. Three dietary treatments were evaluated using broiler chickens: a control without incorporation of rapeseed meal; a second treatment with the incorporation of 3% unfermented rapeseed meal; and a third with the incorporation of 3% rapeseed meal fermented with Bacillus subtilis 67. The study showed that fermented compared to unfermented rapeseed meal was characterized by a significantly higher content of dry matter, crude ash, crude fat and metabolic energy (P < 0.05), and a significantly lower content of crude fiber and glucosinolates (P < 0.05). The B. subtilis 67 strain shows cellulolytic and xylulolytic activity. Fermented rapeseed meal has a positive effect on body weight of birds, daily gain, and European Production Efficiency Factor (P < 0.05). Both rapeseed meal treatments significantly reduced the pH of leg muscles and the water-holding capacity of breast muscles (P < 0.05). The fermented meal had a negative impact on some sensory parameters of poultry meat. There was no significant influence of fermented rapeseed meal on the composition of dipeptides in poultry meat and its antioxidant status.

Recent innovations in various methods of harmful gases conversion and its mechanism in poultry farms.

Poultry breeding takes place in intensive, high-production systems characterized by high animal density, which is a source of harmful emission of odorous volatile organic compounds (VOCs), ammonia (NH3), hydrogen sulfide (H2S) and greenhouse gases, which in turn sustain animal welfare. This study identified and examined the characteristics of chemical compounds emitted in intensive poultry farming (laying hens, broilers) and their toxicity, which led to recommending methods of deodorization. Emphasis was placed on the law relative to air purification in poultry farms. Various methods of air treatment in poultry farms have been described: the modification of animal diet to improve nutrient retention and decrease the amount of their excrement; chemical oxidation technologies (ozonation, photocatalysis, Fenton reaction); various types/brands of biofilters, bioscrubbers and membrane reactors. Numerous studies show that biofilters can reduce ammonia emissions by 51%, hydrogen sulfide by 80%, odors by 67%, while scrubbers brings down ammonia emissions by 77% and odors by 42%, and the application of UV light lowers ammonia emissions by 28%, hydrogen sulfide by 55%, odors by 69% and VOCs by 52%. The paper presents both the solutions currently used in poultry farming and those which are currently in the research and development phase and, as innovative solutions, could be implemented in the near future.

The Use of Selenium Yeast and Phytobiotic in Improving the Quality of Broiler Chicken Meat.

The aim of the present study was to investigate the effect of selenium yeast and phytobiotic on the storage capacity, selected quality parameters of meat and content of selenium in muscles obtained from broilers. In the experiment, 1440 male broiler chickens (Ross 308) were randomly assigned to four research groups: group received no additive (G1), group received a supplement of 0.3 mg Se (as sodium selenite)/kg of feed mixture (G2), group received 0.2 g phytobiotic and 0.3 mg Se as 0.1 g selenium yeast per 1 kg of feed mixture (G3) and group received 0.3 mg Se as 0.1 g selenium yeast per 1 kg of feed mixture (G4). Measurement of pH, determination of water retention capacity, degree of advancement of oxidative changes and selenium content in muscles were performed. Samples of chickens' breast and thigh muscles were microbiologically analyzed. The additives significantly influenced the level of oxidation in muscles and the incorporation of selenium. The meat of chickens receiving organic selenium was characterized by significantly lower dynamics of oxidative changes. The studies carried out showed that selenium in organic form had better absorption.

Isolation and Characterization of Phages Active against Paenibacillus larvae Causing American Foulbrood in Honeybees in Poland.

The aim of this study was the isolation and characterization, including the phage effect on honeybees in laboratory conditions, of phages active against Paenibacillus larvae, the causative agent of American Foulbrood-a highly infective and easily spreading disease occurring in honeybee larva, and subsequently the development of a preparation to prevent and treat this dangerous disease. From the tested material (over 2500 samples) 35 Paenibacillus spp. strains were obtained and used to search for phages. Five phages specific to Paenibacillus were isolated and characterized (ultrastructure, morphology, biological properties, storage stability, and genome sequence). The characteristics were performed to obtain knowledge of their lytic potential and compose the final phage cocktail with high antibacterial potential and intended use of future field application. Preliminary safety studies have also been carried out on healthy bees, which suggest that the phage preparation administered is harmless.

The effect of an enriched laying environment on welfare, performance, and egg quality parameters of laying hens kept in a cage system.

The aim of this study was to evaluate the welfare and performance of laying hens kept in a furnished cage system equipped with additional feeders. A total of 72 Lohmann Brown hens were randomly assigned to 4 experimental groups. Each group consisted of 6 cages housing 3 birds per cage (18 birds per group). Group I was a control group without an extra feeder in the cages. Experimental groups GII, GIII, and GIV contained one, 2, and 3 additional feeders in the cages, respectively. The assessment of bird welfare was based on production, physiological and behavioral parameters, as well as on the basis of external appearance. The experiment lasted 12 wk. The obtained results suggest that enriching laying hens' cages with additional feeders improved the welfare of the hens. Enrichment of cages significantly reduced the number of feather pecking and aggressive behaviors in the GII and GIV groups (P < 0.01) and the GIII group (P < 0.05). Breast plumage was significantly (P < 0.05) better in the GII group compared with that in the control group. The control group also had the worst general plumage (P < 0.01). The GII and GIV groups were also characterized by significantly (P < 0.05) lower blood corticosterone concentrations compared with the control group. No negative changes in egg production and quality parameters were observed in the experimental groups. Only eggs from the GIV group had significantly (P < 0.05) lower breaking strength than those from the control and GII group. The results suggest that the best solution is to place 1 additional feeder in furnished laying hens' cages.

Phages in Therapy and Prophylaxis of American Foulbrood - Recent Implications From Practical Applications.

American foulbrood is one of the most serious and yet unsolved problems of beekeeping around the world, because it causes a disease leading to the weakening of the vitality of honey bee populations and huge economic losses both in agriculture and horticulture. The etiological agent of this dangerous disease is an extremely pathogenic spore-forming bacterium, Paenibacillus larvae, which makes treatment very difficult. What is more, the use of antibiotics in the European Union is forbidden due to restrictions related to the prevention of the presence of antibiotic residues in honey, as well as the global problem of spreading antibiotic resistance in case of bacterial strains. The only available solution is burning of entire bee colonies, which results in large economic losses. Therefore, bacteriophages and their lytic enzymes can be a real effective alternative in the treatment and prevention of this Apis mellifera disease. In this review, we summarize phage characteristics that make them a potentially useful tool in the fight against American foulbrood. In addition, we gathered data regarding phage application that have been described so far, and attempted to show practical implications and possible limitations of their usage.