[Thermotropic behavior of lipids and the morphology of Yersinia pseudotuberculosis cells with a high content of lysophosphatidylethanolamine].

The content of lysophosphatidylethanolamine (LPE) in Y. pseudotuberculosis cells was found to increase during their growth at 8 degrees C under stationary conditions (without stirring the medium) and at 37 degrees C when the medium contained glucose. The maximum level of LPE (up to 45% of the total phospholipids) was observed in cells grown at 8 degrees C under stationary conditions. Such cells showed an enhanced growth rate, a reduced yield of biomass, an altered cell morphology, and an increased cell area. The cells contained unsaturated fatty acids, phosphatidylethanolamine (PE), and total phospholipids in small amounts, whereas neutral lipids and diphosphatidylglycerol were abundant. In addition, the cells contained an amount of methylated PE and phospholipids of unknown structure. Irrespective of whether the temperature for growth was low or high, the LPE-rich cells showed a high value (32-36 degrees C) of the maximum temperature of thermal transition of lipids (Tmax). This finding is indicative of a densification of the membrane lipid matrix of the LPE-rich cells. The suggestion is made that LPE is accumulated in glucose-fermenting bacterial cells in response to stress caused by oxygen deficiency and low pH values of the growth medium. The possible relationship between LPE accumulation and the virulence of Y. pseudotuberculosis cells grown at low temperatures is discussed.

The impact of abiotic factors (temperature and glucose) on physicochemical properties of lipids from Yersinia pseudotuberculosis.

The impact of the availability of glucose in nutrition medium and growth temperature on the composition and thermotropic behavior of lipids from Yersinia pseudotuberculosis (Enterobacteriaceae) was studied. Y. pseudotuberculosis was grown in nutrition broth (NB) with/without glucose at 8 and 37 degrees C, corresponding to the temperatures of saprophytic and parasitic phases of this bacterium life. The decrease of phosphatidylethanolamine, phosphatidylglycerol and unsaturated fatty acids and the parallel increase of lysophosphatidylethanolamine and diphosphatidylglycerol and saturated and cyclopropane acids were the most significant changes with temperature in bacterial phospholipid (PL) classes and fatty acids, respectively. Glucose did not effect the direction of temperature-induced changes in the contents of PLs, fatty acids, however it enhanced (for PLs) or diminished (for fatty acids) intensity of these changes. The thermally induced transitions of lipids were studied by differential scanning calorimetry (DSC). It was revealed that the addition of glucose to NB induced a sharp shift of DSC thermograms to lower temperatures in the "warm" variants of bacteria. The peak maximum temperature (Tmax) of thermal transitions dropped from 50 to 26 degrees C that is the optimal growth temperature of Y. pseudotuberculosis. Tmax of total lipids of the cells grown at 8 degrees C without glucose in NB was equal to growth temperature that corresponded to the classical mechanism of homeoviscous adaptation of bacteria. An addition of glucose to NB at this growth temperature caused the subsequent reduction of Tmax to -8 degrees C, while the temperature ranges of thermograms were not substantially changed. So, not only the temperature growth of bacteria, but also the presence of glucose in NB can modify the physical state of lipids from Y. pseudotuberculosis. In this case, both factors affect additively. It is suggested that glucose influences some membrane-associated proteins and then the fluidity of lipid matrix through temperature-inducible genes.

[Information technologies in public health to simulate the processes of decision making based on medical monitoring data]. / Informatsionnye tekhnologii v zdravookhranenii dlia modelirovaniia protsessov priniatiia reshenii po dannym meditsinskogo monitoringa.

The paper considers how to enhance the efficiency of therapeutical-and-therapeutical procedures and to choose the strategy for arranging medical measures at different levels of public health care, which is largely determined by the corrective use of computer-aided information technologies to apply monitoring approaches to assessing and predicting medical parameters by taking into account the space position of the objects under study and the ecological situation in a region. A procedure is proposed for decision making on the basis of experts' assessments and data imaging findings in arbitrary schematic images. This procedure serves as a basis of making various management decisions.

[Automated complex diagnosis and choice of treatment method in benign prostatic hyperplasia]. / Avtomatizirovannaia kompleksnaia diagnostika i vybor vida lecheniia dobrokachestvennoi giperplazii prostaty.

The paper deals with rational choice of conservative and surgical treatment of patients with benign prostatic hyperplasia (BPH), based on an automatic diagnostic approach and realization of four stages of a logistic model of choice of BPH treatment policy with individual features of the status of urological patients being borne in mind. The procedure was tested in 72 patients from a Voronezh regional clinical hospital of the South-Western Railway, in 92 patients from a Voronezh regional clinical treatment-and-diagnosis center. The values of informative parameters were obtained on the basis of computer-aided patients' self-testing, results of baseline examinations, up-to-date non-invasive studies of the prostate: urofluometry, ultrasound bilocation, MR tomography, etc. The findings lead to the conclusion that integration of computer technologies and up-to-date medical equipment is promising for enhancing the quality of diagnosis and treatment of BPH.