RESUMO
Interactions between different types of immune cells and organically-modified silica nanoparticles were studied. The silica particles functionalized with amine groups were prepared by sol-gel technique. Sheep immunoglobulin labeled with fluoresceine isothiocyanate was immobilized by adsorption onto the nanoparticles. The presence of the functional groups was confirmed by infrared absorption measurements. The level of immunocompetent cells interacting with the silica nanoparticles was estimated as the amount of fluorescence-bright cells by flow cytometry method. A low level of interaction of the peripheral blood lymphocytes with the silica nanoparticles was found. On the contrary, the macrophages are actively involved in interaction with the silica nanoparticles. The influence of different size of the silica nanoparticles and incubation time on viability and functional activity of peripheral blood lymphocytes and peritoneal macrophages were investigated.
Assuntos
Linfócitos/metabolismo , Macrófagos Peritoneais/metabolismo , Nanopartículas/química , Dióxido de Silício/química , Adsorção , Animais , Células Cultivadas , Feminino , Citometria de Fluxo/métodos , Fluoresceína-5-Isotiocianato/química , Corantes Fluorescentes/química , Humanos , Imunoglobulina G/química , Imunoglobulina G/imunologia , Linfócitos/citologia , Macrófagos Peritoneais/citologia , Teste de Materiais , Estrutura Molecular , Tamanho da Partícula , Ovinos , Propriedades de SuperfícieRESUMO
The aim of our work was to establish the peculiarities of phenotype profile and functional activity of peritoneal immunocompetent cells of women with different rate of myoma growth. Forty three women with uterine myoma (main group) and 16 healthy women (control group) were recruited for study. According to the growth rate and tumor's size the main group was divided into two clinical subgroups: (1) women with stable small size of uterine myoma (23 patients), (2) women with large size rapidly growing myoma (20 patients). The expression of CD markers on the surface of peritoneal fluid (PF) lymphocytes and bactericidal and adhesive activity of macrophages were studied. It was found that in women with stable small myomas both lymphocytes and macrophages in PF were activated. In women with rapidly growing large myomas cellular reactions seemed to be inhibited and the impairment of maturation and differentiation of lymphocytes was observed. It can be suggested that local immune response is directly connected with the rate of myoma growth.