RESUMO
The biochemical parameters of hepatobiliary system functions were studied in patients with opisthorchiasis and concomitant diseases, such as chronic viral hepatitis concurrent with chronic opisthorchiasis, as well as Ixodes tick-borne borreliosis in the presence of the same invasion. Although the magnitude ofbiochemical changes is not great in chronic opisthorchiasis or chronic viral hepatitis, the concomitance of these two diseases were ascertained to result in pronounced abnormalities, by demonstrating the exhaustion of spare capacities of the hepatobiliary system in parasitic invasion (or viral infection). When opisthorchiasis was concurrent with Ixodes tickborne borreliosis, some parameters under study differed from those in the groups of patients with monoinfections. Variance analysis showed that chronic opisthorchiasis had a great impact on carbohydrate and lipid metabolisms (glucose and cholesterol levels). The findings suggest that the formation of stable host-parasite relationships in chronic opisthorchiasis alters human metabolic processes and their compensatory capabilities.
Assuntos
Infecções por Borrelia/sangue , Infecções por Borrelia/complicações , Borrelia burgdorferi , Hepatite Viral Humana/sangue , Hepatite Viral Humana/complicações , Opistorquíase/sangue , Opistorquíase/complicações , Opisthorchis , Alanina Transaminase/sangue , Animais , Sistema Biliar/fisiopatologia , Bilirrubina/sangue , Infecções por Borrelia/fisiopatologia , Colesterol/sangue , Colinesterases/sangue , Doença Crônica , Glucose/análise , Glutamil Aminopeptidase/sangue , Hepatite Viral Humana/fisiopatologia , Humanos , Fígado/fisiopatologia , Opistorquíase/parasitologia , Opistorquíase/fisiopatologia , Opisthorchis/fisiologia , gama-Glutamiltransferase/sangueRESUMO
Polymorphonuclear leukocytes from 115 patients with opisthrochiasis at its different (acute and chronic) stages were studied before and 1-3 days, 1-3 months, and 1-2 years after antihelminthic therapy with bilthricide. The functional activity of the cells in question was evaluated following an hour load with different drugs: bilthricide, ascorbic acid, decaris, cyclophosphane, doxycycline in a final concentration of 2.5 x 10(-5) mole/l. Physiological solution and autologous plasma were test controls. The changes in the activity of extracellular (secreted), intracellular (reserve), and summary myeloperoxidase on incubation with each test agent were found to depend on the stage of the disease and the interval after antihelminthic therapy. In early Opisthorchis invasion, the maximum increase in the induced activity of secreted myeloperoxidase is followed by a fall in the level of reserve myeloperoxidase in response to these drugs. With this, there is the most pronounced decrease in reserve myeloperoxidase of polymorphonuclear leukocytes in chronic opisthorchiasis, which may be due to the disrupted controlling processes of its production at late stages of the disease. The distinctive features of the action of agents on the overall activity of granular leukocytic myeloperoxidase in acute and chronic opisthorchiasis suggests that autologous plasma, ascorbic acid, and doxycycline should be used as a test control at the clinical stage of Opisthorchis invasion.
Assuntos
Neutrófilos/metabolismo , Opistorquíase/sangue , Peroxidase/metabolismo , Anti-Helmínticos/farmacologia , Anti-Helmínticos/uso terapêutico , Antinematódeos/farmacologia , Antioxidantes/farmacologia , Ácido Ascórbico/farmacologia , Biomarcadores/análise , Células Cultivadas , Ciclofosfamida/farmacologia , Humanos , Imunossupressores/farmacologia , Levamisol/farmacologia , Neutrófilos/efeitos dos fármacos , Neutrófilos/enzimologia , Opistorquíase/tratamento farmacológico , Opistorquíase/metabolismo , Peroxidase/análise , Praziquantel/farmacologia , Praziquantel/uso terapêutico , Fatores de TempoRESUMO
Cytochemistry was used to study the level of lysosomal cationic proteins of polymorphonuclear peripheral leukocytes in 25 and 20 patients with acute and chronic opisthorchiasis, respectively, before treatment. Comparison groups included patients with chronic viral hepatitis C, bronchial asthma, as well as apparently healthy individuals free of Opisthorchis invasion. That the ratios of an increase in the intracellular content of lysosomal cationic proteins to that in the control individuals were found to be 13.86 and 4.50 for acute and chronic opisthorchiasis, respectively. At the same time, the contribution of an allergic component (bronchial asthma) to the changes in the index studied is greater than that of somatic lesions of the hepatic parenchyma (viral hepatitis C). The authors suggest that it is expedient to use lysosomal cationic proteins in combination with other indices for making a diagnosis and determining the stage of the disease in Opisthorchis invasion.
Assuntos
Proteínas Sanguíneas/análise , Leucócitos Mononucleares/metabolismo , Opistorquíase/metabolismo , Opisthorchis , Animais , Peptídeos Catiônicos Antimicrobianos , Biomarcadores/análise , Proteínas Sanguíneas/metabolismo , Histocitoquímica , Humanos , Opistorquíase/sangue , Opistorquíase/diagnósticoRESUMO
An assay was developed to determine IgM antibodies to Opisthorchis felineus antigens for the diagnosis of acute infection. The method is based on indirect solid-phase enzyme immunoassay. Optimal conditions were elaborated for analysis and its high specificity and specifications are shown in the paper.
Assuntos
Anticorpos Anti-Helmínticos/sangue , Antígenos de Helmintos/imunologia , Imunoglobulina M/sangue , Opistorquíase/diagnóstico , Opisthorchis/imunologia , Doença Aguda , Animais , Humanos , Técnicas Imunoenzimáticas , Sensibilidade e EspecificidadeRESUMO
A total of 152 patients with Opisthorchis infection at the acute and chronic stage of the diseases, as well as 1-3 days and 1-2 months after therapy with biltricide in a dose of 60 mg/kg body weight. Intracellular myeloperoxidase was spectrophotometrically determined. Its activity was demonstrated to be significantly varying at the different opisthorchiasis stage. At the acute stage there was a 6-fold increase in the activity of the enzyme as compared with that at the chronic stage. The therapy resulted in the drop of myeloperoxidase levels, at the same time the authors interpret the heterodirectional changes in the activity of myeloperoxidase in the early post-therapeutic period as a fact of individual anthelmintic intolerance the normalization of the parameter studied 1 month after the therapy only in 42.1% of the cases as a fact residual Opisthorchis antigen persistence.
Assuntos
Antiplatelmínticos/uso terapêutico , Neutrófilos/enzimologia , Opistorquíase/tratamento farmacológico , Opistorquíase/enzimologia , Peroxidase/sangue , Praziquantel/uso terapêutico , Doença Aguda , Biomarcadores/sangue , Doença Crônica , Avaliação de Medicamentos , Humanos , Neutrófilos/efeitos dos fármacos , Peroxidase/efeitos dos fármacos , Espectrofotometria , Fatores de TempoRESUMO
Superinfection of animals having Opisthorchis invasion with Mycobacterium tuberculosis leads to destabilization of host-parasite relationships in opisthorchiasis and formation of new host-parasitocenotic interrelations whose manifestation depends on the phase of the invasive process. At the acute invasive phase of mixed pathology (2 weeks) the activity of the host's immune system increases, while the biological activity of helminths and the number of M. tuberculosis colonies decrease. And on the contrary, at the subacute phase of mixed pathology (2.5 months) the activity of the host's immune system decreases, while the reproductive activity of helminths and the isolation rate of M. tuberculosis from pulmonary tissue increases in comparison with the groups of animals with monoinvasion and monoinfection.
Assuntos
Opistorquíase/parasitologia , Tuberculose/parasitologia , Doença Aguda , Animais , Anticorpos Antibacterianos/sangue , Anticorpos Anti-Helmínticos/sangue , Células Produtoras de Anticorpos/imunologia , Antígenos Heterófilos/imunologia , Cricetinae , Modelos Animais de Doenças , Interações Hospedeiro-Parasita/imunologia , Mesocricetus , Mycobacterium tuberculosis/imunologia , Opistorquíase/imunologia , Opisthorchis/imunologia , Baço/imunologia , Tuberculose/imunologiaRESUMO
7 patients in the acute and 51 patients in the chronic stages of opisthorchiasis were treated with azynoks in daily dose 60 mg per kilo. The radical cure was achieved in 95.8 +/- 0.4% of cases. The wild side effects (headache, vertigo, nausea, fatigue) were registered in 51.7 +/- 9.1% of the cases, predominantly during the first hours of the treatment. The biochemical and immunological changes during the first 2 weeks after azynoks administration were analogues to those seen after biltricide and chloxyl (hexaparaxylol) treatment.