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1.
J Agric Food Chem ; 72(32): 17706-17729, 2024 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-39090814

RESUMO

Numerous studies worldwide have evaluated pesticide residues detected in urine. This review serves as a contribution to this field by presenting an overview of scientific research studies published from 2001 to 2023, including details of study characteristics and research scope. Encompassing 72 papers, the review further delves into addressing key challenges in study design and method used such as sampling and analytical approaches, results adjustments, risk assessment, estimations, and results evaluation. The review explores urinary concentrations and detection frequency of metabolites of organophosphates and pyrethroids, as well as herbicides such as 2,4-D and glyphosate and their metabolites, across various studies. The association of the results with demographic and lifestyle variables were explored. While farmers generally have higher pesticide exposure, adopting organic farming practices can reduce the levels of pesticides detected in their urine. Residence close to agricultural areas has shown high exposure in some cases. Dietary exposure is especially high among people adopting a conventionally grown plant-rich dietary pattern. A higher detection level and frequency of detection are generally found in females and children compared to males. The implications of transitioning to organic and sustainable plant-rich diets for reducing pesticide exposure and potential health benefits for both adults and children require further investigation.


Assuntos
Resíduos de Praguicidas , Resíduos de Praguicidas/urina , Humanos , Feminino , Exposição Ambiental , Masculino , Organofosfatos/urina , Organofosfatos/metabolismo , Piretrinas/urina
2.
Artigo em Inglês | MEDLINE | ID: mdl-38838189

RESUMO

In order to assess compliance with maximum residue levels in foods and evaluate the exposure of the Danish population to pesticides, a comprehensive monitoring programme was conducted. The work from 2012-2017 involved testing pesticide residues in fruits, vegetables, cereals, animal product and processed commodities. The sampling strategy, mainly based on exposure calculations and previous findings, involved the collection of 13,492 samples primarily from fresh conventional and organic produces on the Danish market. The origin of the samples varied, with 34% being of Danish origin and 67% originating from EU and non-EU countries. The results revealed that residues in conventionally grown produce were detected in 54% of the fruit and vegetable samples, and 30% of the cereal samples. Additionally, residues above the maximum residue limits were found in 1.8% of these samples, most frequently in fruits. As previous years, more residues were found in samples of foreign origin compared to samples of Danish origin. Compared to earlier findings more than 40 pesticides were detected for the first time and including boscalid, imidacloprid, thiacloprid, etofenprox, and spinosad, all detected more than 100 times. The data shows that detection of PFAS pesticides has increased dramatically, from 24 in 2006 to 412 in 2022.


Assuntos
Contaminação de Alimentos , Resíduos de Praguicidas , Verduras , Resíduos de Praguicidas/análise , Dinamarca , Contaminação de Alimentos/análise , Verduras/química , Frutas/química , Humanos , Praguicidas/análise
3.
Anal Methods ; 16(23): 3784-3797, 2024 Jun 13.
Artigo em Inglês | MEDLINE | ID: mdl-38828558

RESUMO

Newly designed micro-solid phase extraction cartridges are now available, reflecting the increasing shift towards laboratory automation, especially in the clean-up step for the analysis of pesticide residues in food and feed. In the present study, the introduction of different sorbents on the newly designed PAL µSPE CTC cartridges was investigated for the removal of matrix interferents and the recovery of pesticides. Eight cartridges containing different sorbent combinations and different amounts were used including EMR-lipid (not activated), Z-sep, chitin, C18, PSA, and GCB. The evaluation of co-extractive removal for each cartridge showed that the optimal choice for removing fatty acids was the cartridges containing PSA and Z-sep as clean-up sorbents. However, the presence of C18 and EMR-lipid was still required for the removal of sterols and tocopherols. Two grams of sample, fish feed (FF) and rapeseed cake (RSC) were extracted using QuEChERS citrate buffer, followed by a freeze-out step. The recoveries and repeatability of QuEChERS using µ-SPE clean-up were evaluated for 216 pesticide residues (112 compounds analyzed by GC-MS/MS and 143 compounds by LC-MS/MS, from which 39 compounds were analyzed using both techniques). The best results, with recovery between 70 and 120% and RSD <20%, were achieved when FF samples were cleaned-up with 15 mg EMR-lipid and 20 mg MgSO4. This was achieved for 94% of GC-amenable compounds and 86% of LC-amenable compounds. In the case of RSC, the best results were seen when samples were cleaned-up with the cartridge containing only 20 mg Z-sep and 20 mg MgSO4. This was achieved for 88% of GC-amenable compounds and 90% of LC-amenable compounds. Although these cartridges yielded optimal results in terms of recovery, their use could require more instrument maintenance, especially for GC-MS/MS, due to the lower removal of co-extractives.


Assuntos
Ração Animal , Resíduos de Praguicidas , Microextração em Fase Sólida , Resíduos de Praguicidas/análise , Microextração em Fase Sólida/métodos , Ração Animal/análise , Espectrometria de Massas em Tandem/métodos , Cromatografia Gasosa-Espectrometria de Massas/métodos , Contaminação de Alimentos/análise , Extração em Fase Sólida/métodos , Animais
4.
Artigo em Inglês | MEDLINE | ID: mdl-38039344

RESUMO

Sustainable beer production requires a comprehensive assessment of potential hazards such as pesticides in both the finished product and waste streams, as these streams can be used to create high-value by-products. This study presents the tracking of 13 fungicides (azoxystrobin, boscalid, epoxiconazole, fenpropidin, fenpropimorph, fluquiconazole, flutriafol, fluxapyroxad, kresoxym-methyl, spiroxamine, propiconazole, prothioconazole-desthio, and tebuconazole), two insecticides (chlorpyrifos-methyl and deltamethrin), one herbicide (glyphosate), and one growth regulator (mepiquat) through the beer brewing process. Field-treated rye, wheat, and barley samples containing pesticide residues were used as adjunct during brewing. Samples of the beer as well as the by-products (spent grain, spent hops, trub and spent yeast) were collected and extracted with a modified QuEChERS method for pesticide residues analysis using GC-MS/MS and LC-MS/MS. Results show that an average of 58% of pesticide residues are retrieved in the by-products with the highest fraction (53%) recovered in the spent grain, 4% in trub, 1% in spent hops, no residues detected in spent yeast and 9% in the beer. This is consistent with these nonpolar pesticides tending to remain adsorbed to the spent grain during brewing. Glyphosate and mepiquat, the most polar pesticides included in this study, showed a different behavior, with the largest fraction (>80%) being retrieved in sweet wort and transferred to the beer. Processing factors were generated for each pesticide from the adjunct to the beer and to the four by-products.


Assuntos
Resíduos de Praguicidas , Praguicidas , Resíduos de Praguicidas/análise , Espectrometria de Massas em Tandem , Cromatografia Líquida , Cerveja/análise , Saccharomyces cerevisiae , Praguicidas/análise , Glifosato
5.
Food Chem Toxicol ; 168: 113359, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35970271

RESUMO

The cumulative chronic dietary exposure for the Danish population were assessed using Danish food monitoring data for the period 2012-2017 and Danish food consumption data for the consumer groups children (age 1-2, age 4-6 and age 7-14 years), adults age 15-75, high consumers and consumers eating Danish produced fruit and vegetables whenever possible. Levels below the Limit of Reporting (LOR) were set to ½ LOR with correction. For risk assessment of the cumulative chronic dietary exposure we used the Hazard Index (HI) method where the Hazard Quotient (HQ) was calculated for each pesticide and summed up to a HI. The HI was calculated to be below 1 for all consumer groups; i.e. 0.36 for children age 4-6 years and 0.13 for adults, indicating that the cumulative chronic dietary pesticide exposure is unlikely to constitute a health risk for Danish consumers, even for high consumers eating 600 g or more of fruit and vegetables each day. By eating Danish produced commodities whenever possible, the HI was reduced by a factor of one third for both children and adults. 25 different commodities contributed to about 90% of the HI and to 84% of the exposure.


Assuntos
Resíduos de Praguicidas , Praguicidas , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Dinamarca , Contaminação de Alimentos/análise , Humanos , Lactente , Pessoa de Meia-Idade , Resíduos de Praguicidas/análise , Praguicidas/análise , Praguicidas/toxicidade , Medição de Risco , Verduras , Adulto Jovem
6.
Artigo em Inglês | MEDLINE | ID: mdl-35061573

RESUMO

Upon the European commission's request in 2013, Denmark initiated a survey of the levels of chlorate and perchlorate in agriculture products from the Danish market over a period of 4 years from 2014 to 2017. The results of the survey were used to guide decision making with a view to setting maximum levels for intra-Union trade in 2013 and reviewing and updating the levels from 2020. The results of the Danish survey and the employed analytical method are documented in this paper. In addition to the 89 domestic samples, 30 samples of foreign origin were analysed for chlorate and perchlorate, together with ten samples of baby food and seven samples of infant formula collected from the Danish market. Samples were extracted using the Quick Polar Pesticides extraction method (QuPPe) and analysed on a triple quadrupole LC-MS/MS system. The levels of both chlorate and perchlorate detected in samples originating from Denmark were lower than the current MRLs except for a single sample of lettuce. Moreover, the percentage of foreign samples that were positive for chlorate and perchlorate residues was higher than the percentage of domestic samples. The reason for the generally low levels and low frequency of chlorate and perchlorate in Danish products is most likely linked to the fact that the water supply in Denmark is 100% unchlorinated ground water. The present study shows that residues of perchlorate and chlorate are found in Danish food products at a relatively low frequency and that the levels found are below the EU's MRLs implemented in 2020.


Assuntos
Cloratos , Espectrometria de Massas em Tandem , Cloratos/análise , Cromatografia Líquida/métodos , Dinamarca , Humanos , Fórmulas Infantis/análise , Percloratos/análise , Espectrometria de Massas em Tandem/métodos
7.
J Chromatogr A ; 1652: 462384, 2021 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-34261021

RESUMO

Food analysis is a tremendously broad field that is constantly evolving. New methods have emerged to increase productivity, such as modern miniaturized and robotic analytical techniques. In this paper, a micro-solid-phase extraction system (µ-SPE) for clean-up was combined with a robotic autosampler to yield ready-to-analyze extracts. The system was evaluated for its applicability in routine laboratories. The new, automated, high-throughput µ-SPE clean-up method was applied to acetonitrile extracts and was developed for the analysis of pesticide residues in cereals by gas chromatography-Orbitrap mass spectrometry (GC-Orbitrap-MS). The µ-SPE clean-up efficiency was demonstrated in the removal of matrix-interfering components and in the recovery of pesticides. The sorbent bed mixture consisted of magnesium sulfate, primary-secondary amine, C18, and CarbonX, and effectively retained matrix components without loss of target analytes. Analysis of five types of cereals (barley, oat, rice, rye, and wheat) by GC-Orbitrap-MS showed that the method removed more than 70% of matrix components. The clean-up method was validated for 170 pesticides in rye, 159 pesticides in wheat, 142 pesticides in barley, 130 pesticides in oat, and 127 pesticides in rice. Spike recovery values were 70-120% for all pesticides and the repeatability, calculated as the relative standard deviation, was less than 20%. The limits of quantitation achieved were 0.005 mg kg-1 for almost all analytes, ensuring compliance with the maximum residue limits.


Assuntos
Grão Comestível , Análise de Alimentos , Cromatografia Gasosa-Espectrometria de Massas , Resíduos de Praguicidas , Extração em Fase Sólida , Grão Comestível/química , Análise de Alimentos/métodos , Análise de Alimentos/normas , Resíduos de Praguicidas/análise , Resíduos de Praguicidas/isolamento & purificação , Extração em Fase Sólida/normas
8.
Food Chem ; 356: 129653, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-33812188

RESUMO

Various generic extraction methods have been used to determine pesticide residues, mycotoxins, and polycyclic aromatic hydrocarbons (PAHs) in food and animal feed to ensure consumer safety. However, these methods cannot extract all relevant compounds at an acceptable rate of recovery. This study presents a new extraction method. This new method facilitated the identification of 231 compounds, including 196 pesticides, 11 mycotoxins, and 24 PAHs over a broad range of polarities. These compounds were identified in various sample matrices, including those that are lipid-rich. The processed sample is first extracted with water, acetonitrile, formic acid, and heptane. The addition of ammonium formate results in separation into three phases and enables analysis of the aqueous phase. Solid-phase extraction clean-up procedures were performed as necessary followed by analysis by liquid or gas chromatography and mass spectrometry. Analyte recoveries were typically in the range of 70 - 120% with relative standard deviations below 20%.


Assuntos
Micotoxinas/análise , Resíduos de Praguicidas/análise , Hidrocarbonetos Policíclicos Aromáticos/análise , Ração Animal , Animais , Cromatografia Líquida de Alta Pressão , Alimentos , Análise de Alimentos , Cromatografia Gasosa-Espectrometria de Massas/métodos , Extração em Fase Sólida/métodos
9.
Food Chem Toxicol ; 83: 300-7, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26188179

RESUMO

We used the Hazard Index (HI) method to carry out a cumulative risk assessment after chronic dietary exposure to all monitored pesticides in fruit, vegetables and cereals for various consumer groups in Denmark. Residue data for all the pesticides were obtained from the Danish monitoring programme during the period 2004-2011. Food consumption data were obtained from DANSDA (the DAnish National Survey of Diet and physical Activity) for the period 2005-2008. The calculations were made using three different models to cope with residues below the limit of reporting (LOR). We concluded that a model that included processing factors and set non-detects to ½ LOR, but limited the correction (Model 3), gave the most realistic exposure estimate. With Model 3 the HI was calculated to be 0.44 for children and 0.18 for adults, indicating that there is no risk of adverse health effects following chronic cumulative exposure to the pesticides found in fruit, vegetables and cereals on the Danish market. The HI was below 1 even for consumers who eat more than 550 g of fruit and vegetables per day, corresponding to 1/3 of the population. Choosing Danish-produced commodities whenever possible could reduce the HI by a factor of 2.


Assuntos
Dieta/efeitos adversos , Contaminação de Alimentos , Modelos Biológicos , Resíduos de Praguicidas/toxicidade , Praguicidas/toxicidade , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Dinamarca , Grão Comestível/efeitos adversos , Grão Comestível/química , Grão Comestível/economia , Monitoramento Ambiental , Feminino , Frutas/efeitos adversos , Frutas/química , Frutas/economia , Humanos , Masculino , Pessoa de Meia-Idade , Inquéritos Nutricionais , Resíduos de Praguicidas/análise , Praguicidas/análise , Modelos de Riscos Proporcionais , Medição de Risco , Verduras/efeitos adversos , Verduras/química , Verduras/economia , Adulto Jovem
10.
Food Chem Toxicol ; 55: 113-20, 2013 May.
Artigo em Inglês | MEDLINE | ID: mdl-23333574

RESUMO

The four pesticides epoxiconazole, prochloraz, procymidone and tebuconazole, are commonly used pesticides, all suspected of acting as endocrine disrupters. In the present study, we assessed the acute cumulative dietary exposure to the women of child bearing age and the general population of Denmark to these pesticides from the intake of fruit and vegetables. The assessment was carried out using the probabilistic approach combined with the relative potency factor (RPF) approach. Residue data for prochloraz, procymidone, and tebuconazole were obtained from the Danish monitoring programme 2006-2009, while residue data for epoxiconazole were obtained from the Swedish monitoring programme carried out in the period 2007-2009. Food consumption data were obtained from the Danish nationwide dietary survey conducted in 2000-2002. Relative potency factors for the four pesticides were obtained from rat studies. Prochloraz was used as the index compound. All four pesticides increased nipple retention in male offspring, and epoxiconazole, prochloraz, and tebuconazole also increased the gestation period in pregnant rat dams. For women of childbearing age, the high-end cumulative exposure (99.9th percentile) was calculated to 9% of the Adjusted Reference Value (ARV) for the effect on nipple retention and to 1% of the ARV for the effect on increased gestation period.


Assuntos
Dieta , Disruptores Endócrinos/toxicidade , Exposição Ambiental , Praguicidas/toxicidade , Probabilidade , Dinamarca , Contaminação de Alimentos , Humanos
11.
Reprod Toxicol ; 34(2): 261-74, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22659286

RESUMO

The present study investigated whether a mixture of low doses of five environmentally relevant endocrine disrupting pesticides, epoxiconazole, mancozeb, prochloraz, tebuconazole and procymidone, would cause adverse developmental toxicity effects in rats. In rat dams, a significant increase in gestation length was seen, while in male offspring increased nipple retention and increased incidence and severity of genital malformations were observed. Severe mixture effects on gestation length, nipple retention and genital malformations were seen at dose levels where the individual pesticides caused no or smaller effects when given alone. Generally, the mixture effect predictions based on dose-additivity were in good agreement with the observed effects. The results indicate that there is a need for modification of risk assessment procedures for pesticides, in order to take account of the mixture effects and cumulative intake, because of the potentially serious impact of mixed exposure on development and reproduction in humans.


Assuntos
Antagonistas de Androgênios/toxicidade , Disruptores Endócrinos/toxicidade , Praguicidas/toxicidade , Desenvolvimento Sexual/efeitos dos fármacos , Anormalidades Induzidas por Medicamentos , Animais , Linhagem Celular Tumoral , Interações Medicamentosas , Feminino , Genitália Masculina/anormalidades , Genitália Masculina/efeitos dos fármacos , Humanos , Masculino , Troca Materno-Fetal , Modelos Estatísticos , Gravidez , Ratos , Esteroides/metabolismo
12.
Int J Androl ; 29(1): 186-92, 2006 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-16466539

RESUMO

Prochloraz is an imidazole fungicide that is widely used in Europe, Australia, Asia and South America within gardening and agriculture. Screening studies have shown that prochloraz elicits multiple mechanisms of action in vitro, as it antagonizes the androgen and the oestrogen receptor, agonizes the Ah receptor and inhibits aromatase activity. In vivo prochloraz acts as an antiandrogen in the Hershberger assay by reducing weights of reproductive organs, affecting androgen-regulated gene expressions in the prostate and increasing luteinizing hormone levels. In order to investigate the developmental effects of prochloraz, pregnant Wistar dams were dosed perinatally with 30 mg/kg prochloraz. Results showed that prochloraz significantly reduced plasma and testicular testosterone levels in gestational day 21 male foetuses, whereas testicular progesterone was increased. Gestational length was increased by prochloraz. In male pups a significant increase in nipple retention was found, and the weight of the bulbourethral glands was decreased. Behavioural studies showed that the activity level and sweet preference of adult males were significantly increased, indicating that exposure during gestation and lactation causes permanent effects in adulthood. Overall, these results indicate that prochloraz feminizes the male offspring after perinatal exposure, and that these effects are due, at least in part, to diminished fetal steroidogenesis. Thus, a novel endocrine disruptor has been identified that is mechanistically interesting as it elicits dual mechanisms of action and acts as an antiandrogen both by blocking the androgen receptor and by inhibiting fetal steroidogenesis. That a fungicide with such effects is so widely used is a cause for concern, and its use should be reduced, thereby minimizing the risk of human exposure.


Assuntos
Antagonistas de Androgênios/toxicidade , Disruptores Endócrinos/toxicidade , Fungicidas Industriais/toxicidade , Imidazóis/toxicidade , Antagonistas de Receptores de Andrógenos , Animais , Comportamento Animal/efeitos dos fármacos , Feminização/induzido quimicamente , Fungicidas Industriais/química , Humanos , Imidazóis/química , Masculino , Estrutura Molecular , Tamanho do Órgão/efeitos dos fármacos , Próstata/efeitos dos fármacos , Próstata/metabolismo , Próstata/patologia , Receptores Androgênicos/genética , Reprodução/efeitos dos fármacos , Testículo/anormalidades , Testículo/efeitos dos fármacos , Testículo/patologia
13.
Toxicol Sci ; 85(2): 886-97, 2005 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-15788727

RESUMO

Prochloraz is a commonly used fungicide that has shown multiple mechanisms of action in vitro. It antagonizes the androgen and the estrogen receptors, agonizes the Ah receptor, and inhibits aromatase activity. In vivo prochloraz acts antiandrogenically in the Hershberger assay by reducing weights of reproductive organs, affecting androgen-regulated gene expressions, and increasing luteinizing hormone (LH) levels. The purpose of this study was to investigate reproductive toxic effects after exposure during gestation and lactation to prochloraz alone and a mixture of five pesticides (deltamethrin, methiocarb, prochloraz, simazine, and tribenuron-methyl). Prochloraz (30 mg/kg/day) or the mixture (20 mg/kg/day) was dosed to pregnant Wistar dams from gestational day (GD) 7 until postnatal day (PND) 16. Some dams were taken for cesarean section at GD 21, and others were allowed to give birth. Results showed that prochloraz and the mixture significantly reduced plasma and testicular testosterone levels in GD 21 male fetuses, whereas testicular progesterone was increased. Gestational length was increased by prochloraz. Chemical analysis of the rat breast milk showed that prochloraz was transferred to the milk. In males a significant increase of nipple retention was found, and the bulbourethral gland weight was decreased, whereas other reproductive organs were unaffected. In addition cytochrome P450 (CYP)1A activities in livers were induced by prochloraz, possibly as a result of Ah receptor activation. Behavioral studies showed that the activity level and sweet preference of adult males were significantly increased. Overall these results strongly indicate that prochloraz feminizes the male offspring after perinatal exposure, and that these effects are due, at least in part, to diminished fetal steroidogenesis.


Assuntos
Animais Recém-Nascidos/fisiologia , Feminização/induzido quimicamente , Fungicidas Industriais/toxicidade , Imidazóis/toxicidade , Animais , Comportamento Animal/efeitos dos fármacos , Cesárea , Sistema Enzimático do Citocromo P-450/metabolismo , Feminino , Preferências Alimentares/efeitos dos fármacos , Genitália Masculina/efeitos dos fármacos , Genitália Masculina/crescimento & desenvolvimento , Hormônios Esteroides Gonadais/sangue , Habituação Psicofisiológica/efeitos dos fármacos , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Leite/química , Atividade Motora/efeitos dos fármacos , Mamilos/efeitos dos fármacos , Mamilos/crescimento & desenvolvimento , Tamanho do Órgão/efeitos dos fármacos , Jogos e Brinquedos , Ratos , Ratos Wistar , Sêmen/citologia , Sêmen/efeitos dos fármacos , Maturidade Sexual/efeitos dos fármacos , Paladar/efeitos dos fármacos
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