RESUMO
BACKGROUND: Several species of the genus Cassia are known for their antioxidant, antimicrobial and antidiabetic activities, but some of the lesser-known Cassia species, e.g. C. renigera, C. biflora and C. laevigata have not been studied for their biological activities. RESULTS: Methanol extract of C. laevigata was fractionated by preparative thin-layer chromatography. The resulting six different fractions were tested against Fusarium oxysporum and Aspergillus niger for their antifungal activity. Due to higher antifungal activity of fraction 1 of C. laevigata, this was further analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC), resulting in distinct separation of one compound at a retention time of 7.2 min with an absorbance of 252 nm. Further, this compound was analyzed by gas chromatography-mass spectrometry (GC-MS) for its putative structural identification. Mass spectra of this compound resembled the spectra of anthraquinone 1-carboxylic acid by NIST library search. The genomic-level expression of chalcone synthase, a key enzyme involved in the biosynthesis of polyketides, was increased in C. laevigata when compared to other Cassia species. CONCLUSIONS: This study provides an insight into the higher antifungal activity of C. laevigata, including the identification of anthraquinone 1-carboxylic acid, which may be responsible for the antifungal activity.
Assuntos
Antraquinonas/farmacologia , Antifúngicos/farmacologia , Aspergillus niger/efeitos dos fármacos , Cassia/química , Fusarium/efeitos dos fármacos , Extratos Vegetais/farmacologia , Aciltransferases/genética , Aciltransferases/metabolismo , Antraquinonas/análise , Antifúngicos/análise , Cassia/genética , Cromatografia Líquida de Alta Pressão/métodos , Cromatografia de Fase Reversa/métodos , Cromatografia Gasosa-Espectrometria de Massas/métodos , Estrutura Molecular , Extratos Vegetais/análise , Policetídeos/metabolismo , Especificidade da EspécieRESUMO
Clinically inapparent adrenal masses detected through imaging for nonadrenal disease, often referred to as adrenal incidentalomas, were first described approximately 20 years ago. However, their impact on health outcomes is now better appreciated and gaining broader attention. Despite the rarity of primary endocrine cancers of the adrenal, adrenal masses are one of the most prevalent of all human tumors. The prevalence of adrenal incidentaloma approaches 3% in middle age and increases to as much as 10% in the elderly. This report describes the case of a 30-year-old man who presented primarily with complaints of deep vein thrombosis of the left leg secondary to a nonfunctioning adrenocortical carcinoma.
