Effect of a hay-based diet or different upland grazing systems on milk volatile compounds.

The effect of animal feeding on milk volatile organic compounds (VOCs) of metabolic origin was tested on a hay-based diet (H), a highly diversified pasture under continuous grazing (CG), or a less diversified pasture under rotational grazing (RG). Individual milk of 24 Montbéliarde cows (8 per treatment) were sampled after 2 weeks. Pasture-derived milk was richer (p < 0.05) in camphene, sabinene, ß-caryophyllene, and skatole than H milk. Neither milk yield nor fat content affected the majority of VOCs measured. Skatole increased slightly with milk yield, while indole and cineole decreased slightly with milk fat content but with poor regression (R(2) < 0.54). Multivariate analysis showed that, on the basis of those VOCs of metabolic origin whose concentration differed between treatment (dimethyl-sulfone, skatole, toluene, undecanoic acid, 1-octadecene, benzeneacetaldehyde, octanoic acid, and 2-pentanone-4-hydroxy-4-methyl), it was possible to obtain good discriminations among feeding systems. This study is promising for a future use of VOCs of metabolic origin to trace animal feeding systems.

Severe filamentous fungal infections after widespread tissue damage due to traumatic injury: six cases and review of the literature.

We describe 6 cases of severe filamentous fungal infections after widespread tissue damage due to traumatic injury in previously healthy people. Additionally, we report 69 cases from an exhaustive 20-y review of the literature to investigate the epidemiological and clinical features, the prognosis and the therapeutic management of these post-traumatic severe filamentous fungal infections. Traffic (41%) and farm accidents (25%) were the main causes of injury, which involved either the limbs only (41%) or multiple sites (41%). Necrosis was the main symptom (60%) and Mucorales (72%) and Aspergillus (11%) were the 2 most frequent fungi causing infection. These infections required substantial surgical debridement or amputation (96%) associated with aggressive antifungal therapy (81%), depending on the responsible fungi. This study underlines the need for early, repeated and systematic mycological wound samples to guide and adapt surgical and antifungal management in these filamentous fungal infections.

Surgical outcome of one-stage and two-stage flexor tendon grafting in children.

BACKGROUND: Flexor tendon grafting represents the most common modality for secondary restoration of flexor tendon function. Tendon grafting is either performed in 1 or 2 stages. This study attempts to evaluate the clinical outcome of 1-stage and 2-stage grafting in children. METHODS: A retrospective review was performed identifying 20 children treated for secondary rupture of the flexor digitorum longus by means of a tendon graft. There were 17 boys and 3 girls with a mean age of 10.8 years (range: 3 to 15) at the time of surgery. The preoperative condition of each operated finger was graded by the digital damage classification recommended by Merle and Dautel. Functional status was obtained throughout follow-up using the Strickland classification. RESULTS: There were 10 children in grade 1, 6 grade 2, and 4 grade 3 according to Merle and Dautel classification. The delay between the initial trauma or primary procedure and the secondary surgical procedure averaged 7.5 months for 1-stage grafting and 9 months for 2-stage grafting (range: 1 mo to 2 y). The median Strickland index was 70 (range: 55 to 114) for 1-stage grafting and 66 (range 0 to 103) for 2-stage grafting, which was not statistically different (P=0.1). CONCLUSIONS: The functional outcome seems to depend on the initial severity index. One-stage grafting is a relevant procedure when pulleys are intact and the range of motion is complete. When neurovascular bundles are injured a 1-stage grafting should not be tempted. Satisfactory results are expected with 2-stage grafting providing the principles of this procedure are carefully adhered to. LEVEL OF EVIDENCE: Level 4.

Phospholipid, sphingolipid, and fatty acid compositions of the milk fat globule membrane are modified by diet.

The phospholipid and sphingolipid composition of milk is of considerable interest regarding their nutritional and functional properties. The objective of this article was to determine the lipid composition of the milk fat globule membrane (MFGM) of milk from cows fed a diet rich in polyunsaturated fatty acids. The experiments were performed with 2 groups of 6 cows feeding on (i) maize silage ad libitum (+ grassland hay, mixture of cereals, soyabean meal) or (ii) the maize silage-based diet supplemented with extruded linseed (bringing a lipid proportion of 5% of dry matter). The phospholipid and sphingolipid composition of the MFGM was determined using HPLC/ELSD. The fatty acid (FA) composition of total lipids and phospholipids was determined using GC. As expected, the linseed-supplemented diet decreased the saturated FA and increased the unsaturated FA content in milk fat. MFGM in milk from cows fed the diet rich in polyunsaturated FA resulted in (i) a higher amount of phospholipids (+ 18%), which was related to a smaller size of milk fat globules (ii) an increase of 30% (w/w) of the concentration in sphingomyelin, (iii) a higher content in stearic acid (1.7-fold), unsaturated FA (1.36-fold), and C18:1 trans FA: 7.2 +/- 0.5% (3.7-fold). The MFGM contained a higher concentration of unsaturated FA (C18:1, C18:2, and C18:3) and very long-chain FA (C22:0, C23:0, C24:0, EPA, DHA) compared with total lipids extracted from milk. The technological, sensorial, and nutritional consequences of these changes in the lipid composition of the MFGM induced by dietary manipulation remain to be elucidated.

Monohydrocalcite in calcareous corpuscles of Mesocestoides corti.

Mesocestoides corti (syn. vogae), as many other cestode platyhelminthes, contains abundant mineralized structures called calcareous corpuscles. These concretions may constitute as much as 40% of the dry weight of the organisms, but their function remains poorly understood. In this work, we reviewed the mineral composition of the calcareous corpuscles of M. corti. X-ray diffraction pattern showed that the major mineral component of the corpuscles is a hydrated form of calcium carbonate, monohydrocalcite, also confirmed by infrared spectrometry. The baseline shift of the X-ray diffraction spectra suggested the presence of amorphous calcium carbonate, accordingly to previous reports, and an organic matrix was confirmed by FTIR. Monohydrocalcite is a rare mineral unusually found in biominerals. Although the significance of monohydrocalcite in biominerals has not been determined, the knowledge of corpuscles composition is of relevance to establish their function and for the elucidation of the mechanisms involved in mineralization processes.