Assuntos
Apolipoproteínas B , Doenças Cardiovasculares , HDL-Colesterol , Humanos , Fatores de RiscoRESUMO
BACKGROUND: Tumor necrosis factor-alpha (TNF-α) are considered as a pro inflammatory and interleukin-10 (IL-10) anti inflammatory have been shown to predict the risk of incident of coronary artery disease (CAD). The polymorphism at promoter of TNF-α and IL-10 has been shown to increase transcriptional activity of the gene and play a important role in patho physiology of CAD. Aim of present study is to examine the impact of the TNF-α and IL-10 variant allele on various markers of the CAD and to study its relation with circulating TNF-α and IL-10 levels. METHODS: The -308 G/A & -238 G/A of TNF-α and -1082 G/A & -819 C/T of IL-10 gene polymorphism has been studied in 301 diagnosed CAD subjects (Age 51.50⯱â¯9.28; BMI 25.30⯱â¯3.58) and 305 healthy controls (Age 51.57⯱â¯9.50; BMI 24.06⯱â¯7.26). These polymorphism of TNF-α and IL-10 were detected by real time PCR by using Taqman SNP genotyping assay. Furthermore serum TNF-alpha and IL-10 levels were also measured by ELISA. RESULTS: Allelic and genotypic frequencies did not deviate from Hardy-Weinberg equilibrium in the controls (pâ¯>â¯0.05). On allele contrast, significant association with susceptibility to CAD was detected with polymorphisms in TNF-α -308 G/A, that variant genotype GAâ¯+â¯AA (dominant model) (pâ¯=â¯0.030: ORâ¯=â¯1.61: 95% CIâ¯=â¯1.06-2.44) and variant allele (A) (pâ¯=â¯0.006: ORâ¯=â¯1.71: 95% CIâ¯=â¯1.17-2.51) of TNF-α 308 G/A gene was significant highly observed in the cases as compared to control group. Furthermore, variant genotype CTâ¯+â¯TT (dominant model) (pâ¯=â¯0.004: ORâ¯=â¯1.62: 95% CIâ¯=â¯1.17-2.24) and variant allele (T) (pâ¯<â¯0.001: ORâ¯=â¯1.49: 95% CIâ¯=â¯1.17-1.89) of IL-10 -819 C/T gene was significant highly observed in the cases as compared to control group. CONCLUSION: Our results suggest that the TNF-α G-308A polymorphism independently associated with DBP, cholesterol, triglyceride, LDL, TNF-α and IL-10 levels which may be leads to the development of coronary artery disease of North Indians.
Assuntos
Povo Asiático/genética , Doença da Artéria Coronariana/genética , Predisposição Genética para Doença/genética , Interleucina-10/genética , Polimorfismo de Nucleotídeo Único/genética , Regiões Promotoras Genéticas/genética , Alelos , Biomarcadores , Estudos de Casos e Controles , Feminino , Frequência do Gene/genética , Genótipo , Humanos , Índia , Masculino , Pessoa de Meia-Idade , Fator de Necrose Tumoral alfa/genéticaRESUMO
BACKGROUND/AIM: Cytokines are responsible for the modulation of immunological and inflammatory processes and play a significant role in the pathogenesis of coronary artery disease (CAD). Tumor necrosis factor-alpha (TNF-α) are considered as a pro inflammatory and interleukin-10 (IL-10) anti inflammatory have been shown to predict the risk of incident of CAD. Aim of present study is to examine the impact of the TNF-α and Il-10 levels on various components of the CAD. METHODS: Total 580 subjects were recruited in the present study out of which 290 diagnosed CAD subjects (Age 51.61±9.26; BMI 25.27±3.58) and 290 healthy controls (Age 51.72±9.48; BMI 24.02±7.42). Serum TNF-alpha and IL-10 levels were measured by ELISA. RESULTS: Data of present study revealed that CAD patients had higher frequency (p=0.001) of smoking (38.28%), Alcohol (21.03%), Diabetes (53.45%) and hypertension (48.28%) as compare to controls. Moreover, we have observed highly significant (p<0.05) difference in PR, BMI, DBP, DBP, Glucose, TC, TG, VLDL, LDL, TNF-α and TNF-α/IL-10. However, HDL and IL-10 were found lower in CAD. CONCLUSION: The findings of present study suggest that the TNF-α/IL-10 ratio may play a vital role in the development of CAD of North Indian population.
