RESUMO
OBJECTIVES: Because commensal viruses are defined by the immunologic tolerance afforded to them, any immunomodulation, such as is received during haematopoietic stem-cell transplantation, may shift the demarcation between innocuous viral resident and disease-causing pathogen. METHODS: We analysed by deep-sequencing the plasma virome of 40 allogeneic haematopoietic stem-cell transplantation patients 1 month after transplantation. Because human pegivirus (HPgV) was highly prevalent, we performed a 1-year screening of 122 plasma samples by specific real-time reverse transcription PCR assay. We used the log-rank test and the Gray test to assess association with outcomes, and the Mann-Whitney test and multivariable linear regression model to assess association with T-cell reconstitution. RESULTS: Polyomaviruses (PyV) (20/40 patients), anelloviruses (16/40), pegiviruses (14/40) and herpesviruses (14/40) were most frequently identified, including ten cytomegalovirus; three Epstein-Barr virus; two herpes simplex virus type 1; one human herpesvirus 6b and one human herpesvirus 7; 18 Merkel cell-PyV; two BK-PyV; three PyV-6; and one JC-PyV. Papillomavirus and adenovirus were identified in 11 and two patients, respectively. The HPgV specific real-time reverse transcription PCR screening identified 51 of 122 positive samples, high virus loads and persistent infections up to 1 year after transplantation. Comparison between patients with or without HPgV infection at time of transplantation did not reveal a significant difference in infections, engraftment, survival, graft vs. host disease, relapse or immune reconstitution. CONCLUSIONS: The blood virome after allogeneic haematopoietic stem-cell transplantation includes several DNA viruses, notably herpesviruses and PyV. Among RNA viruses, HPgV is highly prevalent and persists for several months, and it thus may deserve special attention in further research on immune reconstitution.
Assuntos
Vírus de DNA/isolamento & purificação , Transplante de Células-Tronco Hematopoéticas , Vírus de RNA/isolamento & purificação , Adolescente , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto JovemAssuntos
Infecções por Vírus de DNA , Neoplasias Hematológicas/terapia , Transplante de Células-Tronco Hematopoéticas , Torque teno virus , Adolescente , Adulto , Idoso , Aloenxertos , Infecções por Vírus de DNA/sangue , Infecções por Vírus de DNA/epidemiologia , Infecções por Vírus de DNA/etiologia , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
This study analyzes the main parameters that should influence the specific absorption rate (SAR) in children's heads. The evolution of their head shape and the growth of specific parameters, such as the skull thickness, are analyzed. The influence of these parameters on the radio frequency (RF) exposure of children's head is studied. The SAR over 1 g in specific tissue is assessed in different children's head models based on magnetic resonance imaging (MRI) and on non-uniformly down-scaled adult heads. Comparisons with SAR data in adults are reported using a handset with a patch antenna operating at 900 MHz.