RESUMO
Trichinellosis is an emerging parasitic zoonosis in North Vietnam. In this survey, hunted and farm-bred wild boars as well as synanthropic rats were sampled in two provinces of northwest Vietnam where outbreaks of trichinellosis have recently occurred. Evidence of Trichinella infection was studied by parasitological, serological and molecular methods. The results showed relatively low prevalence of Trichinella spiralis in hunted wild boars (2/62 (3.2%; 95% CI: 0.8- 4.8)) and rats (23/820 (2.8%; 95% CI: 13.7-32.3)). Parasite burdens in the muscle tissues were between 0.1 and 0.03 larvae/g, and 0.1 and 7 larvae/g in wild boars and rats, respectively. Seroprevalence in farm-bred wild boars was negative. The findings of Trichinella-infected rats in 7 of the 20 districts of Dien Bien and Son La provinces suggest that the parasite is circulating in these regions. These results indicate that the local population and health centers should be made aware of the risks of eating raw or undercooked meat dishes prepared from wild animals.
Assuntos
Doenças dos Roedores/epidemiologia , Sus scrofa , Doenças dos Suínos/epidemiologia , Triquinelose/epidemiologia , Animais , Anticorpos Anti-Helmínticos/sangue , Ensaio de Imunoadsorção Enzimática , Feminino , Masculino , Músculos/parasitologia , Carga Parasitária , Reação em Cadeia da Polimerase , Prevalência , Ratos , Doenças dos Roedores/parasitologia , Suínos , Doenças dos Suínos/parasitologia , Trichinella/genética , Trichinella/imunologia , Triquinelose/parasitologia , Vietnã/epidemiologiaRESUMO
Trichinellosis is an important emerging or re-emerging zoonotic disease in Southeast Asia. In Vietnam, data on trichinellosis are scarce. Therefore, the present study was designed to determine the seroprevalence of trichinellosis in the domestic lifecycle in two provinces of northwestern Vietnam, where recently isolated outbreaks of human trichinellosis occurred. Serum samples were obtained from 558 pigs, 125 dogs and 98 cats, transported on filter paper, and tested for Trichinella antibodies by ELISA and Western blot, using larval excretory-secretory (E/S) antigens. The overall seroprevalence of antibodies to Trichinella was 5.6%, 4% and 0% in pigs, dogs and cats, respectively. In pigs, positive cases were distributed in 8/20 districts of the two provinces. This study suggests that Trichinella spp. is circulating in the domestic life cycle in northwestern Vietnam. Further study is recommended to investigate the presence of Trichinella in a sylvatic cycle, and to identify the occurring Trichinella species.
Assuntos
Doenças do Gato/parasitologia , Doenças do Cão/parasitologia , Doenças dos Suínos/parasitologia , Triquinelose/veterinária , Animais , Animais Domésticos , Anticorpos Anti-Helmínticos , Western Blotting , Doenças do Gato/epidemiologia , Gatos , Doenças do Cão/epidemiologia , Cães , Ensaio de Imunoadsorção Enzimática/veterinária , Suínos , Doenças dos Suínos/epidemiologia , Triquinelose/epidemiologiaRESUMO
PURPOSE: To estimate the association between the prevalence of epilepsy and potential risk factors in three Burkina Faso villages. METHODS: Three villages were selected based on local reports of high numbers of epilepsy cases and pig-rearing practices. One person aged 7 or older was selected at random from all households of selected concessions for epilepsy screening and blood sampling. Epilepsy was confirmed by a physician using the ILAE definition. The cross-sectional associations between epilepsy and selected factors and seroresponse to the antigens of Taenia solium were estimated using a Bayesian hierarchical logistic regression. Prevalence odds ratios (POR) and their 95% Bayesian Credible Intervals (95% BCI) were estimated. RESULTS: Of 888 individuals interviewed, 39 of 70 screened positive were confirmed to have epilepsy for a lifetime prevalence of 4.5% (95% CI: 3.3; 6.0). The prevalence of epilepsy was associated with a positive reaction to cysticercosis Ag-ELISA serology (POR = 3.1, 95% BCI = 1.0; 8.3), past pork consumption (POR = 9.7, 95% BCI = 2.5; 37.9), and being salaried or a trader compared to a farmer or housewife (POR = 2.9, 95% BCI = 1.2; 6.4). DISCUSSION: Several factors were associated with prevalent epilepsy, with Ag-ELISA suggesting the presence of neurocysticercosis. The association between epilepsy and some occupations may reflect differences in local attitudes toward epilepsy and should be further explored.
Assuntos
Epilepsia/epidemiologia , População Rural , Adolescente , Adulto , Fatores Etários , Antígenos/imunologia , Burkina Faso/epidemiologia , Criança , Estudos Transversais , Cisticercose/sangue , Cisticercose/epidemiologia , Cisticercose/imunologia , Ensaio de Imunoadsorção Enzimática , Epilepsia/sangue , Feminino , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Prevalência , Estudos Retrospectivos , Fatores de Risco , Testes Sorológicos , Inquéritos e Questionários , Adulto JovemRESUMO
Although previous epidemiological surveys in Ecuador indicate the presence of Entamoeba histolytica, prevalence data of this parasite remain scarce. Most of the studies were based on microscopic examination, which does not allow a morphological differentiation from the non-pathogenic Ent. dispar and Ent. moshkovskii. In the present study, 674 stool samples from a South Ecuadorian rural community were screened for Entamoeba spp. Subsequently, molecular identification was performed on 101 samples containing Ent. histolytica/Ent. dispar/Ent. moshkovskii cysts. The study indicated the absence of Ent. histolytica in this South Ecuadorian community and confirmed the difficulty of differentiating Entamoeba spp. based on morphological features.
Assuntos
Entamoeba histolytica/genética , Entamebíase/diagnóstico , Fezes/parasitologia , Adulto , Estudos Transversais , DNA de Protozoário/genética , Equador/epidemiologia , Entamoeba histolytica/patogenicidade , Entamebíase/epidemiologia , Feminino , Humanos , Masculino , Reação em Cadeia da Polimerase , Prevalência , Saúde da População RuralRESUMO
(Neuro)cysticercosis is an important zoonotic disease caused by infection with Taenia solium metacestode larvae. Existing immunodiagnostic techniques detect antibodies and circulating antigens (Ag) in serum and cerebrospinal fluid (CSF). Blood/CSF collection is an invasive procedure associated with blood-borne infections and is often not well accepted by communities. Detection of circulating Ag in urine has been suggested as an alternative, however this has been evaluated in clinical settings only. The aim of the present study was to evaluate the performance of a urine Ag-ELISA under field conditions. Paired serum and urine samples were obtained from participants in endemic areas of Ecuador (n=748) and Zambia (n=690) and were subjected to a monoclonal antibody-based Ag-ELISA. Calculation of positive and negative agreement indices (AI) showed better agreement in the negative direction both for Ecuadorian and Zambian samples (AI of 93.1 and 86.8, respectively). Using a Bayesian approach to determine the test characteristics, similar sensitivities were obtained for serum and urine Ag detection, whereas a decreased specificity was determined for the urine Ag-ELISA with a lower specificity (78.6%) for Zambian samples than for Ecuadorian samples (88.4%). This study indicates a higher specificity for the serum test under field conditions and promotes further research to improve the urine test.
Assuntos
Antígenos de Helmintos/urina , Cisticercose/urina , Taenia solium/isolamento & purificação , Animais , Teorema de Bayes , Cisticercose/diagnóstico , Cisticercose/epidemiologia , Equador/epidemiologia , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Masculino , Sensibilidade e Especificidade , Zâmbia/epidemiologiaRESUMO
A cross-sectional survey on bovine fasciolosis was conducted in the Binh Dinh province of Central Vietnam that was previously identified as hyperendemic for human fasciolosis. In Vietnam, both pure Fasciola gigantica and hybrid and/or introgressed populations of liverflukes bearing genetic material from both Fasciola hepatica and F. gigantica infect humans and animals. In this study, 825 cattle were randomly selected from 8 of the 11 provincial districts for faecal collection; blood samples were taken from 400 of these animals. Fasciola eggs and antibodies against Fasciola were detected by a quantitative sedimentation method and an Enzyme Linked Immunosorbent Assay, respectively. Overall, 54.9% of the animals were shedding Fasciola eggs while 72.2% were Fasciola seropositive. Animals under two years showed lower Fasciola infection rates than older animals. There were no differences in infection rates between districts. These results indicate a very high prevalence of Fasciola infections in cattle in Binh Dinh province. It is concluded that a fasciolosis control programme should be designed in this region aiming at reducing infection in both cattle and humans.
Assuntos
Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/parasitologia , Fasciola/isolamento & purificação , Fasciolíase/veterinária , Animais , Anticorpos Antiprotozoários/sangue , Sangue/parasitologia , Bovinos , Estudos Transversais , Doenças Endêmicas , Ensaio de Imunoadsorção Enzimática , Fasciola/classificação , Fasciola/genética , Fasciolíase/epidemiologia , Fasciolíase/parasitologia , Fezes/parasitologia , Humanos , Parasitologia/métodos , Vietnã/epidemiologiaRESUMO
Neurocysticercosis (NCC), caused by the pork tapeworm Taenia solium, is reported to be a common condition in Nepal. So far imaging diagnosis was mainstay of the diagnosis. In this paper, we report three patients presenting with neurological symptoms due to intraventricular NCC. We have diagnosed the causative agent as T. solium on molecular basis. Further research is warranted to assess the actual health impact of T. solium in Nepal.
Assuntos
Ventrículos Cerebrais/parasitologia , Neurocisticercose/diagnóstico , Taenia solium/isolamento & purificação , Adolescente , Idoso , Animais , Anticorpos Anti-Helmínticos/análise , Ventriculografia Cerebral/métodos , Diagnóstico Diferencial , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Taenia solium/imunologia , Tomografia Computadorizada por Raios XRESUMO
Neurocysticercosis is recognised as an important but neglected cause of epilepsy in developing countries where the parasite occurs. Data on the transmission dynamics of the parasite in endemic areas are scarce. Individuals living in these areas are likely to be highly exposed to the parasite, but relatively few of them develop active infections. The present study aimed to describe and gain insights into changes in antibody responses and infection patterns related to age and/or gender in a south Ecuadorian rural population by combining antibody and antigen serological data with demographic characteristics. In 25% of the population, antibodies to Taenia solium cysticerci were detected whilst 2.9% had circulating parasite antigens. The proportion of antibody-positive individuals increased significantly until the age of 40years to become stable in older individuals. A rule-based simulation model was developed to explain these variations and to reflect the dynamics of exposure to, and transmission of, the parasite. In contrast, the proportion of people presenting circulating parasite antigens, reflecting an active infection, was significantly higher in people older than 60years. Immunosenescence could explain such an observation since a weaker immune system in the elderly would facilitate the establishment and maintenance of viable cysticerci compared with fully immunocompetent younger individuals. This work points out the role of the immune system in the development of cysticercosis within an exposed population and highlights new essential issues in understanding the transmission dynamics of the parasite, its incidence and the resulting immunological response at a population level.
Assuntos
Anticorpos Anti-Helmínticos/sangue , Antígenos de Helmintos/sangue , Cisticercose , População Rural , Taenia solium/imunologia , Adolescente , Adulto , Distribuição por Idade , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Animais , Criança , Pré-Escolar , Cisticercose/diagnóstico , Cisticercose/epidemiologia , Cisticercose/parasitologia , Cisticercose/transmissão , Equador/epidemiologia , Humanos , Lactente , Recém-Nascido , Pessoa de Meia-Idade , Prevalência , Distribuição por Sexo , Suínos , Taenia solium/isolamento & purificação , Adulto JovemRESUMO
Parasitic food-borne diseases are generally underrecognised, however they are becoming more common. Globalization of the food supply, increased international travel, increase of the population of highly susceptible persons, change in culinary habits, but also improved diagnostic tools and communication are some factors associated with the increased diagnosis of food-borne parasitic diseases worldwide. This paper reviews the most important emerging food-borne parasites, with emphasis on transmission routes. In a first part, waterborne parasites transmitted by contaminated food such as Cyclospora cayetanensis, Cryptosporidium and Giardia are discussed. Also human fasciolosis, of which the importance has only been recognised in the last decades, with total numbers of reported cases increasing from less than 3000 to 17 million, is looked at. Furthermore, fasciolopsiosis, an intestinal trematode of humans and pigs belongs to the waterborne parasites as well. A few parasites that may be transmitted through faecal contamination of foods and that have received renewed attention, such as Toxoplasma gondii, or that are (re-)emerging, such as Trypanosoma cruzi and Echinococcus spp., are briefly reviewed. In a second part, meat-borne parasite infections are reviewed. Humans get infected by eating raw or undercooked meat infected with cyst stages of these parasites. Meat inspection is the principal method applied in the control of Taenia spp. and Trichinella spp. However, it is often not very sensitive, frequently not practised, and not done for T. gondii and Sarcocystis spp. Meat of reptiles, amphibians and fish can be infected with a variety of parasites, including trematodes (Opisthorchis spp., Clonorchis sinensis, minute intestinal flukes), cestodes (Diphyllobothrium spp., Spirometra), nematodes (Gnathostoma, spp., anisakine parasites), and pentastomids that can cause zoonotic infections in humans when consumed raw or not properly cooked. Another important zoonotic food-borne trematode is the lungfluke (Paragonimus spp.). Traditionally, these parasitic zoonoses are most common in Asia because of the particular food practices and the importance of aquaculture. However, some of these parasites may emerge in other continents through aquaculture and improved transportation and distribution systems. Because of inadequate systems for routine diagnosis and monitoring or reporting for many of the zoonotic parasites, the incidence of human disease and parasite occurrence in food is underestimated. Of particular concern in industrialised countries are the highly resistant waterborne protozoal infections as well as the increased travel and immigration, which increase the exposure to exotic diseases. The increased demand for animal proteins in developing countries will lead to an intensification of the production systems in which the risk of zoonotic infections needs to be assessed. Overall, there is an urgent need for better monitoring and control of food-borne parasites using new technologies.
Assuntos
Doenças Transmissíveis Emergentes/parasitologia , Parasitologia de Alimentos , Helmintíase , Infecções por Protozoários , Zoonoses/parasitologia , Animais , HumanosRESUMO
In this study, packed cell volume-values (PCV) are evaluated as indicator of trypanosomiasis infections in cattle. A total of 734 blood samples were collected in 11 different sampling sites in eastern Zambia: 84 calves (< 1 year), 52 young females and 40 young males (between 1 and 3 years), 228 cows, 317 oxen and 13 bulls (> 3 years). All samples were subjected to three diagnostic tests: parasitological examination using the buffy coat method, PCR/RFLP and PCV determination. The results were compared and analysed in a Bayesian model, which allowed the estimation of the infection prevalence and the respective test sensitivities and specificities. The presence of a trypanosomal infection significantly reduced the PCV, independently of the age and sex of the infected animal. The estimated prevalence of trypanosomal infections in the study area was 34% (95% credibility interval: 30-38%). While the specificity of both the parasitological and the PCR/RFLP tests were set to 1, the parasitological diagnosis had a low sensitivity (37%) compared to the PCR/RFLP (96%). When using a cut-off value of 24, the PCV had a high specificity (98%) but a rather low sensitivity (53%) for identifying trypanosomiasis infections. Using 26% as a cut-off increased the sensitivity to 76% without much affecting the specificity (94%). A parallel combination of the parasitological diagnosis and the PCV improved the diagnostic sensitivity (74% and 89% for PCV cut-off values of 24% or 26%, respectively) while specificity remained high (98% and 94% for PCV cut-off values of 24% or 26%, respectively). These results suggest that such a combination could advantageously be used for the diagnosis of cattle trypanosomiasis in the field: it is much more sensitive than parasitological examination alone and it is much cheaper than molecular tests. However, the value of this approach depends largely on the determination of an appropriate cut-off value to consider a sample positive, depending on the required test sensitivities and specificities.
Assuntos
Doenças dos Bovinos/sangue , Tripanossomíase/veterinária , Animais , Bovinos , Doenças dos Bovinos/diagnóstico , Doenças dos Bovinos/epidemiologia , Feminino , Hematócrito/veterinária , Masculino , Sensibilidade e Especificidade , Tripanossomíase/sangue , Tripanossomíase/diagnóstico , Tripanossomíase/epidemiologia , Zâmbia/epidemiologiaRESUMO
The pork tapeworm, Taenia solium, causative organism of porcine cysticercosis and human neurocysticercosis is known to occur in areas of South Africa including Eastern Cape Province but, despite increasing reports of its occurrence throughout the subregion, the prevalence is yet to be clearly established. The parasite presents a potentially serious agricultural problem and public health risk in endemic areas. The human populations considered to be at highest risk of infection with this zoonotic helminth are people living in rural areas most of whom earn their livelihood wholly or partially through livestock rearing. Here we report on initial results of a community-based study of pigs owned by resource-poor, emerging pig producers from 21 villages in the Eastern Cape Province. Lingual examination (tongue palpation) in live pigs, two enzyme-linked immunosorbent assays (ELISAs), which detect parasite antigen (B158/B60 Ag-ELISA and HP10 Ag-ELISA) and an enzyme immunotransfer blot (EITB) assay, which detects antiparasite antibody, were used to verify endemicity and estimate apparent prevalence. In the absence of a gold standard true prevalence was obtained, using a Bayesian approach, with a model that uses both available data and prior information. Results indicate that the parasite is indeed present in the study villages and that true prevalence was 64.6%. The apparent prevalences as measured by each of the four tests were: 11.9% for lingual examination, 54.8% for B158/B60 Ag-ELISA, 40.6% for HP10 Ag-ELISA and 33.3% for EITB. This base-line knowledge of the prevalence of T. solium in pigs provides information essential to the design and monitoring of sustainable and appropriate interventions for cysticercosis prevention and control.
Assuntos
Infecções Comunitárias Adquiridas/veterinária , Cisticercose/veterinária , Doenças dos Suínos/parasitologia , Taenia solium , Animais , Infecções Comunitárias Adquiridas/sangue , Infecções Comunitárias Adquiridas/parasitologia , Cisticercose/sangue , Cisticercose/epidemiologia , Prevalência , África do Sul/epidemiologia , Suínos , Doenças dos Suínos/sangue , Doenças dos Suínos/epidemiologia , Língua/parasitologia , Língua/patologiaRESUMO
The diagnostic performance of six foot-and-mouth disease (FMD) assays for detection of antibodies to the non-structural proteins (NSP) of the FMD virus (FMDV) was estimated using a Bayesian analysis on field sera from cattle of unknown infection status originating from post-FMDV outbreak situations in Israel and Zimbabwe. Estimations of the disease prevalence in both populations were also obtained. The diagnostic sensitivity estimates did not differ between both field studies, although overall Bayesian estimates were markedly higher than those previously reported based on sera from comparable experimentally infected (vaccinated) cattle populations. All NSP-based assays demonstrated a lower diagnostic specificity when applied to the Zimbabwean sera compared to both published specificities and similar Bayesian specificity estimates derived for the Israeli dataset. In Israel, the disease prevalence was estimated at 23.9% (95% credibility interval: 19.5-28.8%), whereas 65.4% (59.0-72.5%) was found in Zimbabwe. The need for reliable diagnostic test performance estimates and the benefits of Bayesian analysis in obtaining them are also addressed.
Assuntos
Anticorpos Antivirais/sangue , Antígenos Virais , Ensaio de Imunoadsorção Enzimática/métodos , Febre Aftosa/diagnóstico , Febre Aftosa/epidemiologia , Proteínas não Estruturais Virais , Animais , Teorema de Bayes , Bovinos , Febre Aftosa/imunologia , Israel/epidemiologia , Prevalência , Sensibilidade e Especificidade , Zimbábue/epidemiologiaRESUMO
In spite of the EU directives that regulate meat inspection for bovine cysticercosis, Taenia saginata is still present in Europe and causes economic losses due to condemnation, refrigeration and downgrading of infected carcasses. The main reasons for this persistence include the low sensitivity of current meat inspection protocols, the dissemination and survival of eggs in the environment and cattle husbandry systems, which allow grazing on pastures and drinking from water streams. It is assumed that water streams and surface water are potentially contaminated with T. saginata eggs. Furthermore, current wastewater management not only fails to halt, but rather contributes to the dissemination of eggs in the environment. Here, the authors discuss an integrated approach for control of this food-borne zoonosis, as well as the potential use of serological methods as a way of improving detection of bovine cysticercosis.
Assuntos
Taenia saginata/fisiologia , Teníase/epidemiologia , Teníase/prevenção & controle , Animais , Bovinos , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/parasitologia , Cisticercose/epidemiologia , Europa (Continente)/epidemiologia , Parasitologia de Alimentos , Humanos , Prevalência , Teníase/parasitologiaRESUMO
The alternative pathway of complement is an important innate defence against pathogens including ticks. This component of the immune system has selected for pathogens that have evolved countermeasures. Recently, a salivary protein able to inhibit the alternative pathway was cloned from the American tick Ixodes scapularis (Valenzuela et al., 2000; J. Biol. Chem. 275, 18717-18723). Here, we isolated two different sequences, similar to Isac, from the transcriptome of I. ricinus salivary glands. Expression of these sequences revealed that they both encode secreted proteins able to inhibit the complement alternative pathway. These proteins, called I. ricinus anticomplement (IRAC) protein I and II, are coexpressed constitutively in I. ricinus salivary glands and are upregulated during blood feeding. Also, we demonstrated that they are the products of different genes and not of alleles of the same locus. Finally, phylogenetic analyses demonstrate that ticks belonging to the Ixodes ricinus complex encode a family of relatively small anticomplement molecules undergoing diversification by positive Darwinian selection.
Assuntos
Proteínas Inativadoras do Complemento/química , Ixodes/química , Proteínas e Peptídeos Salivares/química , Sequência de Aminoácidos , Animais , Evolução Biológica , Proteínas Inativadoras do Complemento/genética , Proteínas Inativadoras do Complemento/metabolismo , Feminino , Imuno-Histoquímica , Ixodes/genética , Ixodes/metabolismo , Dados de Sequência Molecular , Família Multigênica , Glândulas Salivares/metabolismo , Homologia de Sequência de AminoácidosRESUMO
Taenia solium-taeniasis and cysticercosis were studied in the human and porcine populations of a rural community in the Southern Ecuadorian Andes. From the 1059 inhabitants, 800 serum samples and 958 stool samples could be collected. In addition, 646 from the estimated 1148 pigs were tongue inspected. Circulating antigen was detected by enzyme linked immunosorbent assay (Ag-ELISA) in 2.25% of the human population, whereas intestinal taeniasis was detected in 1.46% by the formalin-ether technique. Following treatment and recovery of tapeworm fragments these were all identified as T. solium. Porcine cysticercosis was diagnosed in 3.56% of the pigs by tongue inspection. In addition, enzyme linked immunoelectrotransfer blot (EITB) was performed on a subset group of 100 humans to confirm the results of the Ag-ELISA. One hundred serum samples from pigs were also analysed by EITB. It appeared that 43 and 74% of humans and pigs had antibodies against T. solium cysticerci, respectively. It is concluded that contrary to the high exposure of the human population to T. solium that is suggested by EITB, the number of active cysticercosis cases, diagnosed by Ag-ELISA, was low, which may indicate endemic stability. The further use of complementary diagnostic methods for a better understanding of the epidemiology of T. solium is suggested.
Assuntos
Anticorpos Anti-Helmínticos/sangue , Doenças Endêmicas , Taenia solium/isolamento & purificação , Teníase/diagnóstico , Adolescente , Adulto , Idoso , Animais , Criança , Cisticercose/diagnóstico , Cisticercose/epidemiologia , Cisticercose/veterinária , Equador/epidemiologia , Fezes/parasitologia , Humanos , Programas de Rastreamento , Pessoa de Meia-Idade , Polimorfismo de Fragmento de Restrição , Prevalência , População Rural , Estações do Ano , Suínos , Doenças dos Suínos/diagnóstico , Doenças dos Suínos/epidemiologia , Doenças dos Suínos/parasitologia , Taenia solium/genética , Taenia solium/imunologia , Teníase/epidemiologia , Teníase/veterináriaRESUMO
Taenia solium-taeniasis and cysticercosis were studied in the human and porcine populations of a rural community in the Southern Ecuadorian Andes. From the 1059 inhabitants, 800 serum samples and 958 stool samples could be collected. In addition, 646 from the estimated 1148 pigs were tongue inspected. Circulating antigen was detected by enzyme linked immunosorbent assay (Ag-ELISA) in 2.25 percent of the human population, whereas intestinal taeniasis was detected in 1.46 percent by the formalin-ether technique. Following treatment and recovery of tapeworm fragments these were all identified as T. solium. Porcine cysticercosis was diagnosed in 3.56 percent of the pigs by tongue inspection. In addition, enzyme linked immunoelectrotransfer blot (EITB) was performed on a subset group of 100 humans to confirm the results of the Ag-ELISA. One hundred serum samples from pigs were also analysed by EITB. It appeared that 43 and 74 percent of humans and pigs had antibodies against T. solium cysticerci, respectively. It is concluded that contrary to the high exposure of the human population to T. solium that is suggested by EITB, the number of active cysticercosis cases, diagnosed by Ag-ELISA, was low, which may indicate endemic stability. The further use of complementary diagnostic methods for a better understanding of the epidemiology of T. solium is suggested.