Nitrogen-functionalized graphene quantum dot incorporated GelMA microgels as fluorescent 3D-tissue Constructs.

Biopolymer microgels present many opportunities in biomedicine and tissue engineering. To understand their in vivo behavior in therapeutic interventions, long-term monitoring is critical, which is usually achieved by incorporating fluorescent materials within the hydrogel matrix. Current research is limited due to issues concerning the biocompatibility and instability of the conventional fluorescent species, which also tend to adversely affect the bio-functionality of the hydrogels. Here, we introduce a microfluidic-based approach to generate nitrogen-functionalized graphene quantum dot (NGQD) incorporated gelatin methacryloyl (GelMA) hydrogel microspheres, capable of long-term monitoring while preserving or enhancing the other favorable features of 3D cell encapsulation. A multilayer droplet-based microfluidic device was designed and fabricated to make monodisperse NGQD-loaded GelMA hydrogel microspheres encapsulating skeletal muscle cells (C2C12). Control over the sizes of microspheres could be achieved by tuning the flow rates in the microfluidic device. Skeletal muscle cells encapsulated in these microgels exhibited high cell viability from day 1 (82.9 ± 6.50%) to day 10 (92.1 ± 3.90%). The NGQD-loaded GelMA microgels encapsulating the cells demonstrated higher metabolic activity compared to the GelMA microgels. Presence of sarcomeric α-actin was verified by immunofluorescence staining on day 10. A fluorescence signal was observed from the NGQD-loaded microgels during the entire period of the study. The investigation reveals the advantages of integrating NGQDs in microgels for non-invasive imaging and monitoring of cell-laden microspheres and presents new opportunities for future therapeutic applications.

Excitation dependence and independence of photoluminescence in carbon dots and graphene quantum dots: insights into the mechanism of emission.

Excitation-dependent, multicolor emission from different varieties of 0D carbon systems has attracted immense research attention. It is generally accepted that some variants of 0D carbon exhibit excitation dependent emission, while other variants do not. A third variant exhibits both excitation dependent as well as excitation independent emission. In this work we investigate the structure, composition, steady-state emission-excitation and photoluminescence decay dynamics of three distinctly different variants of 0D carbon - amorphous carbon dots (aCDs), graphene quantum dots (GQDs) and nitrogen-doped GQDs (NGQDs). We find that despite significant differences in the structure and composition there is a striking similarity in the excitation energy dependence of the emission characteristics of these three different dots. All of them exhibit excitation energy independent emission below some threshold wavelength (λth), and above this threshold the emission becomes excitation dependent. We also demonstrate that a similar trend is apparent for nearly all variants of 0D carbon reported in the literature. The threshold wavelength correlates well with the excitation wavelength for the most intense emission and the photoluminescence excitation peaks, suggesting a common origin of light emission in these carbon dots. The findings provide important clues for developing a unified general picture for understanding the light emission mechanism in 0D carbon nanostructures.

One-Pot Synthesis of Gel Glass Embedded with Luminescent Silicon Nanoparticles.

Preparation of highly luminescent glasses involves expensive and complicated processes and usually requires high temperature. In this work, we show that luminescent silicon (Si) nanoparticle (NP)- embedded silicate gel glasses can be developed under near-ambient conditions by a remarkably simple, one-pot strategy, without using any sophisticated instrumentation or technique. Simultaneous hydrolysis and reduction of (3-aminopropyl)triethoxysilane leads to the formation of colloidal Si nanocrystals that can be transformed to a glassy phase upon slow evaporation followed by freezing. Structural investigations reveal the formation of a sodium silicate gel glass framework having discernible shear bands, along with embedded Si NPs. High photoluminescence quantum yield (ca. 35-40%), low glass-transition temperature ( Tg ≈ 66-73 °C), strain-tolerant mechanical stability, and inexpensive preparation make the glass attractive for applications as display materials and photonic converters.

Amorphous Carbon Dots and their Remarkable Ability to Detect 2,4,6-Trinitrophenol.

Apparently mundane, amorphous nanostructures of carbon have optical properties which are as exotic as their crystalline counterparts. In this work we demonstrate a simple and inexpensive mechano-chemical method to prepare bulk quantities of self-passivated, amorphous carbon dots. Like the graphene quantum dots, the water soluble, amorphous carbon dots too, exhibit excitation-dependent photoluminescence with very high quantum yield (~40%). The origin and nature of luminescence in these high entropy nanostructures are well understood in terms of the abundant surface traps. The photoluminescence property of these carbon dots is exploited to detect trace amounts of the nitro-aromatic explosive - 2,4,6-trinitrophenol (TNP). The benign nanostructures can selectively detect TNP over a wide range of concentrations (0.5 to 200 µM) simply by visual inspection, with a detection limit of 0.2 µM, and consequently outperform nearly all reported TNP sensor materials.

Biomimetically synthesized polymer-hydroxyapatite sheet like nano-composite.

Biomimetic internal architecture is proving valuable for multi-tissue and structural tissue interface engineering. A biomimetic method developed for the in situ synthesis of hydroxyapatite in poly (vinyl) alcohol, produces nanocomposites in the form of fabric-like sheets, when subjected to lyophilisation. The surface reactive hydroxyl groups of poly (vinyl) alcohol encourage site-specific coupling of hydroxyapatite. This results in improved mechanical properties, which may be better for biomedical applications when compared to a mere blend of the above two.

Hydroxyapatite coating on stainless steel pre-coated with bovine serum albumin at ambient conditions.

A biomimetic process for coating of nanosized hydroxyapatite on stainless steel, which capitalises the dual nature of the protein bovine serum albumin in both metal binding and a strong affinity for calcium ions, has been developed. The novelty of the process lies in pre-conditioning the metallic surface using the above protein prior to its mineralization with hydroxyapatite at ambient conditions. The microporous morphology of these coatings may provide favourable solubility and resorbability as desired by many orthopaedic and orthodontic applications.