Assessing the Risk Perception and Knowledge Regarding Cardiovascular Diseases in Patients With Hypertension and Diabetes in Central India: A Mixed-Methods Study.

Background Cardiovascular diseases (CVDs) are a major cause of morbidity and mortality worldwide. The perceptions of patients can be important in health-related behaviors and disease prognosis. Thus, this study aimed to determine the risk perception and knowledge of hypertensive and diabetic patients. Methodology The study was conducted among 264 patients with diabetes and/or hypertension. A sequential exploratory mixed-methods design was used, which initially identified themes related to risk perception about CVDs among patients. Knowledge and risk perception about CVDs were quantified using a survey derived from predefined themes. CVD knowledge scores were categorized into low, intermediate, and high scores, and the trends of risk perception were studied across this spectrum of cardiovascular knowledge. Results The age of the participants ranged from 30 to 78 years. Overall, 57.19% of the participants were males, whereas 42.80% were females. The qualitative results revealed that the sources of knowledge about CVDs, physical activity, and maintaining a nutritious diet included family, friends, and media. On the other hand, doctors played a significant role in influencing perceptions related to medications. The observation of complications related to CVDs and the fear of mortality played a pivotal role in shaping the perception regarding the seriousness of the condition. Perceived susceptibility was low if there was unawareness of protection through medications and lifestyle changes, but it was high with stress or heredity. The analysis of CVD knowledge and risk perception survey data revealed the median CVD knowledge score to be 12 (interquartile range = 7.5-13), which showed a significant association with education and socioeconomic status (p < 0.05). The distribution of risk perception scores across the spectrum of CVD knowledge scores depicted that participants with higher scores agreed more with CVDs being serious and their susceptibility to them. Conclusions Despite having high knowledge scores regarding CVDs, the study population had average risk perception. Patient risk perceptions should be evaluated as it affects the health-seeking behavior and prognosis of the disease.

Prevalence and Correlates of Depression Among School-Going Adolescents in the Urban Schools of Central India: A Cross-Sectional Study.

BACKGROUND: Adolescence is defined as the phase of development that occurs between childhood and adulthood. Presently in India, 243 million populations are staring at the crossroads of transition from childhood to adulthood. Physical, emotional, and social issues unique to this age group make them vulnerable to various mental problems. So, we conducted this study to quantify the current burden of depression in adolescents and its possible causes. MATERIALS AND METHODS: The present community-based cross-sectional study was conducted in the middle and late adolescent participants aged 14-19 years from 52 sections (clusters) of 9th to 12thclasses comprising a total of 1412 students with a multistage cluster sampling method. In total four sections (clusters), and one participant of class 9th, 10th, 11th, and 12th were chosen from 13 preselected schools. The questionnaire consisted of socio-demographic details, screen time, physical activity, etc., and the DASS-42 scale was used to determine the prevalence of depression.  Results: We found that the prevalence of depression in our study participants was around 39%. It was classified as 16.9%, 16.7%, 5.1%, and 0.5% participants respectively having mild, moderate, severe, and extremely severe depression. Mother's education was a statistically significant determinant for depression among these adolescents. CONCLUSION: The study concludes that the prevalence of depression (including mild, moderate, severe, or very severe) among school-going adolescents is 39%. We hereby recommend that a holistic approach should be followed involving parents and teachers with the help of school counselors to tackle and curb this problem.

Three-dimensional Assessment of Two-rooted Maxillary Central Incisor with Labiogingival Groove: Endodontic and Periodontal Surgical Management.

Understanding the diversity of root canal systems and their anatomical/morphological variations helps in achieving improved outcomes of root canal treatment. The outcome of endodontic treatment depends on a thorough knowledge of dental anatomy and its variations. Maxillary central incisor with two roots is a rare entity, so a complete clinical and radiological examination is important before initiating the treatment. Recently, three-dimensional (3D) radiography has improved the diagnosis and treatment planning in endodontics. So, the current report describes the endodontic and periodontal surgical management of traumatized discolored maxillary central incisors with two separated roots and root canals identified by two-dimensional (2D) and 3D radiographic examination. How to cite this article: Prasad P, Galani M, Nawal RR, et al. Three-dimensional Assessment of Two-rooted Maxillary Central Incisor with Labiogingival Groove: Endodontic and Periodontal Surgical Management. Int J Clin Pediatr Dent 2023;16(3):528-533.

Adjunctive Integrated Stress Response Inhibition Accelerates Tuberculosis Clearance in Mice.

Despite numerous advances in tuberculosis (TB) drug development, long treatment durations have led to the emergence of multidrug resistance, which poses a major hurdle to global TB control. Shortening treatment time therefore remains a top priority. Host-directed therapies that promote bacterial clearance and/or lung health may improve the efficacy and treatment duration of tuberculosis antibiotics. We recently discovered that inhibition of the integrated stress response, which is abnormally activated in tuberculosis and associated with necrotic granuloma formation, reduced bacterial numbers and lung inflammation in mice. Here, we evaluated the impact of the integrated stress response (ISR) inhibitor ISRIB, administered as an adjunct to standard tuberculosis antibiotics, on bacterial clearance, relapse, and lung pathology in a mouse model of tuberculosis. Throughout the course of treatment, ISRIB robustly lowered bacterial burdens compared to the burdens with standard TB therapy alone and accelerated the time to sterility in mice, as demonstrated by significantly reduced relapse rates after 4 months of treatment. In addition, mice receiving adjunctive ISRIB tended to have reduced lung necrosis and inflammation. Together, our findings identify the ISR pathway as a promising therapeutic target with the potential to shorten TB treatment durations and improve lung health. IMPORTANCE Necrosis of lung lesions is a hallmark of tuberculosis (TB) that promotes bacterial growth, dissemination, and transmission. This process is driven by the persistent hyperactivation of the integrated stress response (ISR) pathway. Here, we show that adjunctive ISR inhibition during standard antibiotic therapy accelerates bacterial clearance and reduces immunopathology in a clinically relevant mouse model of TB, suggesting that host-directed therapies that de-escalate these pathological stress responses may shorten TB treatment durations. Our findings present an important conceptual advance toward overcoming the challenge of improving TB therapy and lowering the global burden of disease.

Improved bladder cancer antitumor efficacy with a recombinant BCG that releases a STING agonist.

Despite the introduction of several new agents for the treatment of bladder cancer (BC), intravesical BCG remains a first line agent for the management of non-muscle invasive bladder cancer. In this study we evaluated the antitumor efficacy in animal models of BC of a recombinant BCG known as BCG-disA-OE that releases the small molecule STING agonist c-di-AMP. We found that compared to wild-type BCG (BCG-WT), in both the orthotopic, carcinogen-induced rat MNU model and the heterotopic syngeneic mouse MB-49 model BCG-disA-OE afforded improved antitumor efficacy. A mouse safety evaluation further revealed that BCG-disA-OE proliferated to lesser degree than BCG-WT in BALB/c mice and displayed reduced lethality in SCID mice. To probe the mechanisms that may underlie these effects, we found that BCG-disA-OE was more potent than BCG-WT in eliciting IFN-ß release by exposed macrophages, in reprogramming myeloid cell subsets towards an M1-like proinflammatory phenotypes, inducing epigenetic activation marks in proinflammatory cytokine promoters, and in shifting monocyte metabolomic profiles towards glycolysis. Many of the parameters elevated in cells exposed to BCG-disA-OE are associated with BCG-mediated trained innate immunity suggesting that STING agonist overexpression may enhance trained immunity. These results indicate that modifying BCG to release high levels of proinflammatory PAMP molecules such as the STING agonist c-di-AMP can enhance antitumor efficacy in bladder cancer.

Re-engineered BCG overexpressing cyclic di-AMP augments trained immunity and exhibits improved efficacy against bladder cancer.

In addition to its role as a TB vaccine, BCG has been shown to elicit heterologous protection against many other pathogens including viruses through a process termed trained immunity. Despite its potential as a broadly protective vaccine, little has been done to determine if BCG-mediated trained immunity levels can be optimized. Here we re-engineer BCG to express high levels of c-di-AMP, a PAMP recognized by stimulator of interferon genes (STING). We find that BCG overexpressing c-di-AMP elicits more potent signatures of trained immunity including higher pro-inflammatory cytokine responses, greater myeloid cell reprogramming toward inflammatory and activated states, and enhances epigenetic and metabolomic changes. In a model of bladder cancer, we also show that re-engineered BCG induces trained immunity and improved functionality. These results indicate that trained immunity levels and antitumor efficacy may be increased by modifying BCG to express higher levels of key PAMP molecules.

Spatio-temporal assessment of COVID-19 lockdown impact on beach litter status and composition in Goa, India.

The study aimed to understand beach litter status at some of the world-famous beaches of Goa, West India, to comprehend the impact of the Covid-19 lockdown. We characterize litter in six categories (Nylon+Rubber, Plastics, Footwear, Glass, Metal, and Thermocol) for eight sampled beaches in the north and south Goa. All beaches show increased glass and decreased plastics (significant litter) during the lockdown period compared to the unlock period that marked the high tourist inflow. Beaches were classified and graded with colour codes using litter density exhibit light blue-green colour coding during the lockdown or unlock period, suggests clean maintenance. The Miramar beach located in the heart of the capital city showed relatively more litter density (yellow code) due to the combination of local people and tourist inflow. Morjim, Palolem, Velsao were littered the least during both periods.

Suitability of Google Trends™ for Digital Surveillance During Ongoing COVID-19 Epidemic: A Case Study from India.

OBJECTIVE: Digital surveillance has shown mixed results as a supplement to traditional surveillance. Google Trends™ (GT) (Google, Mountain View, CA, United States) has been used for digital surveillance of H1N1, Ebola and MERS. We used GT to correlate the information seeking on COVID-19 with number of tests and cases in India. METHODS: Data was obtained on daily tests and cases from WHO, ECDC and covid19india.org. We used a comprehensive search strategy to retrieve GT data on COVID-19 related information-seeking behavior in India between January 1 and May 31, 2020 in the form of relative search volume (RSV). We also used time-lag correlation analysis to assess the temporal relationships between RSV and daily new COVID-19 cases and tests. RESULTS: GT RSV showed high time-lag correlation with both daily reported tests and cases for the terms "COVID 19," "COVID," "social distancing," "soap," and "lockdown" at the national level. In 5 high-burden states, high correlation was observed for these 5 terms along with "Corona." Peaks in RSV, both at the national level and in high-burden states corresponded with media coverage or government declarations on the ongoing pandemic. CONCLUSION: The correlation observed between GT data and COVID-19 tests/cases in India may be either due to media-coverage-induced curiosity, or health-seeking curiosity.

Prevalence of Hypothyroidism in Pregnant Women in India: A Meta-Analysis of Observational Studies.

INTRODUCTION: This meta-analysis was conducted to estimate the prevalence of hypothyroidism among pregnant women in India. METHODS: We searched PubMed, Web of Science, Scopus, Google Scholar, and Shodhganga (Indian thesis repository) for observational studies, providing prevalence of hypothyroidism among pregnant women in India. Systematic study selection and data extraction procedures were followed. Quality assessment of each study was done using JBI critical appraisal checklist. The random effects model was used for pooling the effect sizes. Publication bias was assessed using the funnel plot and rank correlation test. I 2 statistics was used to measure heterogeneity across the studies. Heterogeneity in the pooled estimates was further explored with subgroup analyses and meta-regression analysis. RESULTS: Sixty-one studies were found eligible and included in this review. The pooled estimate of the prevalence of hypothyroidism in pregnant women was 11.07% (95% CI: 8.79-13.84, I 2 = 99%). Pooled prevalence estimates of subclinical and overt hypothyroidism are 9.51% (95% CI: 7.48-12.04, I 2 = 98%) and 2.74% (95% CI: 2.08-3.58, I 2 = 94%). CONCLUSION: We documented 11.07% pooled prevalence of hypothyroidism in pregnant women in India.

Risk factors for undernutrition in under-five children living in a migrant populated area of South Delhi.

INTRODUCTION: Undernutrition is continuing to be a major public health problem in India. Moreover, India houses more than 30% of the world's stunted children aged under 5 years. There was a paucity of a community-based cross-sectional study on the nutrition of children aged under 5 years in urban Delhi. Therefore, we conducted this study to assess the prevalence of undernutrition and their associated factors in children aged under 5 years in urban South Delhi. MATERIALS AND METHODS: A cross-sectional community-based study was conducted in the Mehrauli area of South Delhi with a semi-structured questionnaire. Systematic random sampling without replacement was implemented. Informed written consent was sought from the parents of participants. Questions were asked regarding demography and sociocultural factors of the participants. Anthropometry measurements were obtained. Underweight and stunting were defined using WHO child growth standards 2006. RESULTS: A total of 520 participants in 695 houses were contacted and interviewed. Prevalence of underweight and stunting were 34.0% and 42.6%, respectively. Sociocultural factors, associated with underweight and stunting, found in current study were fathers' education (with both, P value < 0.05), mothers' education (with both, P value < 0.05), poverty status (with both, P value <0.05), overcrowding (stunting only, P value <0.05), fathers' alcohol abuse (with both, P value < 0.05) and exclusive breastfeeding for 6 months (stunting only, P value <0.05). RESULTS: A total of 520 participants in 695 houses were contacted and interviewed. Prevalence of underweight and stunting were 34.0% and 42.6%, respectively. Sociocultural factors, associated with underweight and stunting, found in current study were fathers' education (with both, P value < 0.05), mothers' education (with both, P value < 0.05), poverty status (with both, P value <0.05), overcrowding (stunting only, P value <0.05), fathers' alcohol abuse (with both, P value < 0.05) and exclusive breastfeeding for 6 months (stunting only, P value <0.05). CONCLUSIONS: There was a very high prevalence of underweight (34.0%) and stunting (42.6%) in children under 5 years age in Mehrauli.

Screening for diabetes among tuberculosis patients registered under revised national tuberculosis control program, Bhopal, India.

CONTEXT: Tuberculosis (TB) and diabetes mellitus (DM) remain a global public health problem. India has the largest number of TB cases; in 2015, out of total global annual incidence of 9.6 million TB cases, 2.2 million were estimated from India. There are 62.4 million people with type 2 diabetes and 77 million people with prediabetes in India, and these numbers are projected to increase to 101 million by the year 2030. Diabetes and TB affect each other at many levels. Screening for diabetes in patients with TB will not only help in early case detection but also better management of both comorbidities. AIMS: (i) To determine the prevalence of diabetes and prediabetes among diagnosed cases of TB registered under RNTCP in Bhopal district. (ii) To determine additional yield of previously unknown DM and the number needed to screen (NNS) to find out a new case of DM. (iii) To find out the factors associated with diabetes among patients diagnosed with TB registered under RNTCP in Bhopal district. SETTINGS AND DESIGN: This study was a cross-sectional study conducted on registered patients with TB under RNTCP in two TB units of Bhopal district. MATERIALS AND METHODS: Participants were contacted and interview was conducted after obtaining consent using predesigned and pretested performa during the period of 1st October 2014 to 30th March 2015 for a period of 6 months. STATISTICAL ANALYSIS USED: Continuous variable were summarized as frequency, mean, and standard deviation. All variables were analyzed using Chi-square test of significance; P < 0.05 was taken as statically significant. RESULT: Of the total 528 patients with TB, 296 was male and 232 were female. Of the total, 63 (11.9%) patients were diagnosed as diabetic. NNS to diagnose a new case of DM was 22.1. Significant association was found with six variables which are age, sex, body mass index, type of TB, category of TB, and smoking. CONCLUSION: This study shows feasibility and importance of screening of patients with TB in existing program settings.

Hypoxia-Mediated Epigenetic Regulation of Stemness in Brain Tumor Cells.

Activation of pluripotency regulatory circuit is an important event in solid tumor progression and the hypoxic microenvironment is known to enhance the stemness feature of some cells. The distinct population of cancer stem cells (CSCs)/tumor initiating cells exist in a niche and augment invasion, metastasis, and drug resistance. Previously, studies have reported global hypomethylation and site-specific aberrant methylation in gliomas along with other epigenetic modifications as important contributors to genomic instability during glioma progression. Here, we have demonstrated the role of hypoxia-mediated epigenetic modifications in regulating expression of core pluripotency factors, OCT4 and NANOG, in glioma cells. We observe hypoxia-mediated induction of demethylases, ten-eleven-translocation (TET) 1 and 3, but not TET2 in our cell-line model. Immunoprecipitation studies reveal active demethylation and direct binding of TET1 and 3 at the Oct4 and Nanog regulatory regions. Tet1 and 3 silencing assays further confirmed induction of the pluripotency pathway involving Oct4, Nanog, and Stat3, by these paralogues, although with varying degrees. Knockdown of Tet1 and Tet3 inhibited the formation of neurospheres in hypoxic conditions. We observed independent roles of TET1 and TET3 in differentially regulating pluripotency and differentiation associated genes in hypoxia. Overall, this study demonstrates an active demethylation in hypoxia by TET1 and 3 as a mechanism of Oct4 and Nanog overexpression thus contributing to the formation of CSCs in gliomas. Stem Cells 2017;35:1468-1478.