Optimization parameters for the production of dimer of epicatechin from an endophytic fungus Curvularia australiensis FC2AP using response surface methodology (RSM).

The search for natural therapeutic agents has intensified due to their potential to treat various diseases. Bioactive secondary metabolites from endophytes offer high therapeutic profiles and can be mass-produced after optimizing medium parameters and purification. This investigation aimed to maximize crude pigmented secondary metabolite (CPSM) production from Curvularia australiensis FC2AP by optimizing fermentation conditions statistically. The endophytic fungus produced a maximum yield of 8.81 UL/g from biomass using Sabouraud's Dextrose Broth. After screening essential factors, the Plackett-Burman design was used for factorial optimization, and the Box Behnken design was employed to investigate three significant factors. The final CPSM yield was 12.3 UL/g, approximately 4-fold higher than the preliminary growth medium. Chromatographic purification using a gradient solvent system resulted in six fractions, with the fourth fraction demonstrating the highest bioactivity profile. Structural characterization confirmed this fraction to be a dimer of epicatechin, which has anti-cancer properties, as confirmed through in vivo studies on Sprague Dawley rats. This is the first report of a epicatechin dimer produced from C. australiensis.

Banana peels as a cost effective substrate for fungal chitosan synthesis: optimisation and characterisation.

Massive accumulation of unprocessed banana peels enthralls sustainable issues as they are eventually dumped as landfills leading to emission of obnoxious gasses. To avoid these persisting challenges the present study shims lights on chitosan production from the characterised fungal strain using banana peel hydrolysate as an effective medium. Substantial amount of carbohydrate in banana peels serves as a potential solution for fungal chitosan production in a view to attain a circular bioeconomy and repurposed for synthesis of beneficial products in a cost effective manner. Presence of fermentable sugars in banana peels qualifies them as a feasible substrate which could be exploited for scaling up of fungal chitosan synthesis. Screened isolate was subjected to statistical optimisation using formulated medium to elucidate the influential factors that had significant effect on chitosan production. The harvested chitosan biomass was characterised through standardised techniques and evaluated for further studies. Statistical optimisation reveals that ammonium nitrate (5 g/L), pH (6) and incubation time (144 hrs) were the three PBD variables that had a greater influence on fungal chitosan yield. The validated developed model exhibited maximum yield of 200 mg/L, a 4.4 fold increase than unoptimised medium (45 mg/L). These findings emphasise the fermentative synthesis of chitosan through valorisation of banana peel prop up a complementary approach in concomitant with preserving renewable resources and bioproduct formation.

Evaluation of Dimer of Epicatechin from an Endophytic Fungus Curvularia australiensis FC2AP on Acute Toxicity Levels, Anti-Inflammatory and Anti-Cervical Cancer Activity in Animal Models.

Cervical cancer, as the most frequent cancer in women globally and accounts almost 14% in India. It can be prevented or treated with vaccines, radiation, chemotherapy, and brachytherapy. The chemotherapeutic agents cause adverse post effects by the destruction of the neighboring normal cells or altering the properties of the cells. In order to reduce the severity of the side effects caused by the chemically synthesized therapeutic agents, the current research developed an anti-cancer agent dimer of epicatechin (DoE), a natural bioactive secondary metabolite (BSM) mediated from an endophytic fungus Curvularia australiensis FC2AP. The investigation has initiated with the evaluation of inhibiting the angiogenesis which is a main activity in metastasis, and it was assessed through Hen's Egg Test on Chorio Allantoic Membrane (HET-CAM) test; the BSM inhibited the growth of blood vessels in the developing chick embryo. Further the DoE was evaluated for its acute toxicity levels in albino mice, whereas the survival dose was found to be 1250 mg/kg and the lethal dose was 1500 mg/kg body weight of albino mice; hematological, biochemical, and histopathological analyses were assessed. The anti-inflammatory responses of the DoE were evaluated in carrageenan induced Wistar rats and the reduction of inflammation occurred in a dose-dependent manner. By fixing the effective dose for anti-inflammation analysis, the DoE was taken for the anti-cervical cancer analysis in benzo (a) pyrene induced female Sprague-Dawley rats for 60 days trial. After the stipulated days, the rats were taken for hematological antioxidants, lipid peroxidation (LPO), member bound enzymes, cervical histopathological and carcinogenic markers analyses. The results specified that the DoE has the capability of reducing the tumor in an efficient way. This is the first report of flavonoid-DoE production from an endophytic fungus C. australiensis has the anticancer potentiality and it can be stated as anti-cancer drug.

Bioprocess optimization and production of biosurfactant from an unexplored substrate: Parthenium hysterophorus.

Biosurfactants are one among the best alternative for synthetic surfactants that are exploited by many researchers. Several agro wastes help to reduce the cost biosurfactants by being renewable and economical. The present research focuses on the biosurfactant production from Pseudomonas mosselii utilizing Parthenium hysterophorus as a relatively cheap substrate. P. hysterophorus being a hazardous weed, its eradication is quite tedious. So, the utilization of the weed for useful purposes serves as a choice to overcome the problems posed by the weed. In the study, this weed has been successfully utilized as a substrate and the optimized fermentative production of biosurfactant was done. From one-factor-at a-time analysis it was known that the substrate level of 3% incubation time of 96 h, pH 6.0, temperature 35 °C, glucose and yeast extract was found to be the best C and N sources for a high yield. The extracted biosurfactant was partially purified and characterized using FTIR. The biosurfactant produced from the weed could help to render the milestone for distinct biomedical and other applications.