Glucocorticoid and triiodothyronine concentrations do not correlate with behavior in vicuñas (Vicugna vicugna).

State-dependent foraging theory posits that animals should make foraging decisions based on energetic condition, where animals with fewer energetic reserves prioritize foraging over other behaviors, including antipredator behaviors. However, few studies have investigated these trade-offs at an individual level in wild, free-ranging animals. We investigated the relationships between internal condition and behavior in a wild mammal, the vicuña (Vicugna vicugna), which makes state-dependent decisions about the use of two habitats with different characteristics that contribute to their internal condition. Using non-invasively collected fecal samples, we measured glucocorticoid metabolites (GCMs) and thyroid hormones (THs) as indicators of combined stress (predation and nutritional), and just nutritional stress, respectively. We video recorded 20-minute behavioral observations and focused on behaviors which often demand a trade-off between energy acquisition and antipredator behaviors-vigilance and foraging. We found differences in expression of these behaviors between the two sites but found no relationships between physiological parameters (GCMs and THs) and behavior (vigilance and foraging) at either site. We suggest that state-dependent foraging may be difficult to observe in large mammals under baseline conditions and that GCMs and THs may be insensitive to small changes in stress stimuli at this scale, and where these wild animals have the entire suite of behavioral responses available to them.

Overfishing and nutrient pollution interact with temperature to disrupt coral reefs down to microbial scales.

Losses of corals worldwide emphasize the need to understand what drives reef decline. Stressors such as overfishing and nutrient pollution may reduce resilience of coral reefs by increasing coral-algal competition and reducing coral recruitment, growth and survivorship. Such effects may themselves develop via several mechanisms, including disruption of coral microbiomes. Here we report the results of a 3-year field experiment simulating overfishing and nutrient pollution. These stressors increase turf and macroalgal cover, destabilizing microbiomes, elevating putative pathogen loads, increasing disease more than twofold and increasing mortality up to eightfold. Above-average temperatures exacerbate these effects, further disrupting microbiomes of unhealthy corals and concentrating 80% of mortality in the warmest seasons. Surprisingly, nutrients also increase bacterial opportunism and mortality in corals bitten by parrotfish, turning normal trophic interactions deadly for corals. Thus, overfishing and nutrient pollution impact reefs down to microbial scales, killing corals by sensitizing them to predation, above-average temperatures and bacterial opportunism.

Nutrient supply from fishes facilitates macroalgae and suppresses corals in a Caribbean coral reef ecosystem.

On coral reefs, fishes can facilitate coral growth via nutrient excretion; however, as coral abundance declines, these nutrients may help facilitate increases in macroalgae. By combining surveys of reef communities with bioenergetics modeling, we showed that fish excretion supplied 25 times more nitrogen to forereefs in the Florida Keys, USA, than all other biotic and abiotic sources combined. One apparent result was a positive relationship between fish excretion and macroalgal cover on these reefs. Herbivore biomass also showed a negative relationship with macroalgal cover, suggesting strong interactions of top-down and bottom-up forcing. Nutrient supply by fishes also showed a negative correlation with juvenile coral density, likely mediated by competition between macroalgae and corals, suggesting that fish excretion may hinder coral recovery following large-scale coral loss. Thus, the impact of nutrient supply by fishes may be context-dependent and reinforce either coral-dominant or coral-depauperate reef communities depending on initial community states.