Effect of bimatoprost sustained-release intracameral implant on intraocular pressure and medication burden in patients with prior glaucoma surgery.

The present retrospective study evaluated intraocular pressure (IOP) and medication burden after bimatoprost sustained-release (bimatoprost SR, Durysta, Allergan) implantation in patients with glaucoma. A secondary objective was to examine an effect of bimatoprost SR in a subset of patients with prior minimally invasive and incisional glaucoma surgery. A retrospective chart review of 122 eyes that received bimatoprost SR by 6 glaucoma specialists at Wills Eye Hospital between March 2020 and September 2021 was performed. One hundred and eighteen eyes from 84 patients had a reduction in IOP (18.5±5.7mmHg vs. 16.0±5.4mmHg, P<0.01) and required fewer glaucoma medications (2.1±1.4 vs. 1.2±1.2, P<0.01) after bimatoprost SR implantation. In 41 eyes from 31 patients who previously underwent glaucoma surgery (including iStent, goniotomy, trabeculectomy, Xen Gel Stent, or tube shunt surgery), medication burden was decreased after bimatoprost SR implantation (1.9±1.3 vs. 1.0±1.0, P<0.001). These data suggest that bimatoprost SR is an efficacious treatment modality for glaucoma, even in post-surgical patients.

Non-steroidal drug-induced glaucoma.

Numerous systemically used drugs are involved in drug-induced glaucoma. Most reported cases of non-steroidal drug-induced glaucoma are closed-angle glaucoma (CAG). Indeed, many routinely used drugs that have sympathomimetic or parasympatholytic properties can cause pupillary block CAG in individuals with narrow iridocorneal angle. The resulting acute glaucoma occurs much more commonly unilaterally and only rarely bilaterally. CAG secondary to sulfa drugs is a bilateral non-pupillary block type and is due to forward movement of iris-lens diaphragm, which occurs in individuals with narrow or open iridocorneal angle. A few agents, including antineoplastics, may induce open-angle glaucoma. In conclusion, the majority of cases with glaucoma secondary to non-steroidal medications are of the pupillary block closed-angle type and preventable if the at-risk patients are recognized and treated prophylactically.

The relationship between climate and the distribution of Culicoides imicola in Iberia.

The biting midge Culicoides imicola was captured at 17 of 27 farms in Spain and Portugal during a survey of its distribution following outbreaks of African horse sickness in Iberia that occurred between 1987 and 1990. Farms were sampled approximately twice weekly from October 1992 to February 1995. Farms were widely spaced apart (maximum 850 km) and had considerable variation in climate. Across sites, summer temperatures ranged from 18.3 degrees C-27.2 degrees C; in winter the range was 4.4 degrees C-11.6 degrees C. Relative humidities in summer ranged from 37.2% to 90.1%. Proximity to southern Spain (Seville) was the most significant predictor of the presence/absence of C. imicola, but high summer temperatures and possibly dry summer conditions, were also important. Vila Nova de Milfontes in Portugal, where C. imicola was abundant and the climate is relatively cool, was an exception to the climatic trends at the other 26 sites. This exception points to a lack of knowledge of climatic requirements for immature development of C. imicola. The absence of C. imicola from the three most easterly sites, which have apparently favourable climates, suggests a relatively recent invasion by this species into Iberia.

The seasonal and geographical distribution of Culicoides imicola, C. pulicaris group and C. obsoletus group biting midges in central and southern Spain.

Pirbright-type light traps were used to collect Culicoides biting midges (Diptera: Ceratopogonidae) at fifteen sites in twelve provinces of central Spain and Andalusia. A total of 293,625 Culicoides were collected in 1,387 samples over a two year period. These comprised approximately 9.2% Culicoides imicola, 11.4% C. pulicaris group, 1.6% C. obsoletus group and 12.2% C. circumscriptus. Culicoides imicola was present at ten of the fifteen sites; the five sites from which it was absent were the most eastern of the fifteen. The greatest abundance of this species was at Navalmoral in Caceres Province. Culicoides pulicaris group were present at all sites; C. obsoletus group were present at twelve sites. The annual peaks in abundance were: C. imicola, August-October; C. pulicaris group, May-June; and C. obsoletus group, March-June. The geographical and seasonal distributions of C. imicola are consistent with those of the outbreaks of African horse sickness (AHS) and bluetongue (BT) during epizootics in Spain, and support the contention that C. imicola was the major vector of AHS and BT viruses.

Spatial and seasonal distribution of Culicoides imicola in Iberia in relation to the transmission of African horse sickness virus.

Collections of biting midges were made over 24 months from sixty sites spread across Iberia. Information on the distribution of the vector of African horse sickness virus, Culicoides imicola, from these 3119 samples showed that this species was annually present across south-western Spain as far as 3 degrees 53'W and throughout most of Portugal, up to 41 degrees 5'N. C. imicola was found in all areas where African horse sickness epizootics had occurred in 1987-90 and also in areas outside the epizootic zones. Seasonal patterns of capture success of C. imicola, from seventeen frequently sampled sites where the vector was present, usually showed a late summer-early autumn peak. At the sites furthest south there was a discrete peak, mostly in September or October, before and after which the numbers captured increased or decreased steadily. At higher latitudes peak abundances occurred as early as May or as late as November, population build up was less uniform and numbers often declined rapidly after the peak was reached. Both the distribution and seasonal abundance patterns closely matched transmission patterns of African horse sickness virus, which rose during late summer and caused most cases during the autumn months.

Transformation of sterigmatocystin and O-methylsterigmatocystin by aflatoxigenic and nonaflatoxigenic field isolates of the Aspergillus flavus group.

Transformation of sterigmatocystin and O-methylsterigmatocystin (two metabolic aflatoxin precursors) to aflatoxins by aflatoxigenic and nonaflatoxigenic field isolates of Aspergillus flavus was studied. The 24 nonaflatoxigenic isolates investigated failed to transform both precursors. Among the 8 aflatoxin-producing isolated used, 7 transformed both precursors whereas the remaining failed to transform both. According to these results, the usefulness of the measurement of enzymatic activities related to aflatoxin production in understanding the true status of conflictive field isolates is discussed.

Considerations on the distribution of aflatoxigenic Aspergillus flavus in feeds.

The distribution of aflatoxin producing isolates of the Aspergillus flavus group in feeds was studied. Aflatoxin production was investigated by a sequential method previously reported (fluorescence in Coconut Agar Medium, rapid extraction from a wheat medium, and total extraction from the same wheat medium). Twenty-seven of 32 samples contained A. flavus, and 21 of them had at least one aflatoxicogenic isolate of A. flavus. Of the 115 isolates analysed, 65 produced aflatoxins, mainly B aflatoxins.