RESUMO
As found out in earlier investigations, after the intratracheal infection of guinea pigs with C. burnetii (the method of infection, most similar to aerogenic infection which is characteristic of Coxiella under natural conditions) the maximum amount of the infective agent is accumulated in the lungs of the animals, damaging the organs of the reticuloendothelial system. The present investigation has revealed that this method of infection makes it possible differentiate C. burnetii virulent strains, differing in the level of the accumulation of the infective agent in the lungs of guinea pigs.
Assuntos
Coxiella burnetii/patogenicidade , Febre Q/microbiologia , Animais , Coxiella burnetii/isolamento & purificação , Cobaias , Pulmão/microbiologia , Linfonodos/microbiologia , Ratos , Baço/microbiologia , Fatores de Tempo , Traqueia , VirulênciaRESUMO
Immunization of horses with Ebola virus resulted in production of specific virus-neutralizing antibodies with their maximal level attained on days 28 to 42 postimmunization. Repeated cycles of immunization lead to increase of antibodies titer to 1:4096.
Assuntos
Ebolavirus/imunologia , Soros Imunes/biossíntese , Animais , Anticorpos Antivirais/biossíntese , Cavalos , Soros Imunes/imunologia , Testes de NeutralizaçãoAssuntos
Vírus Lassa/imunologia , Vacinas Virais/isolamento & purificação , Animais , Chlorocebus aethiops , Relação Dose-Resposta Imunológica , Avaliação Pré-Clínica de Medicamentos , Imunização/métodos , Febre Lassa/prevenção & controle , Papio , Fatores de Tempo , Vacinas de Produtos Inativados/imunologia , Vacinas de Produtos Inativados/isolamento & purificação , Células Vero , Vacinas Virais/imunologia , Viremia/prevenção & controleRESUMO
Immunization of horses with Ebola virus resulted in the production of specific virus-neutralizing antibody with maximum titres at 28-42 days. Repeated cycles of immunization led to a rise in antibody titres to 1:4096.
Assuntos
Ebolavirus/imunologia , Soros Imunes/isolamento & purificação , Animais , Anticorpos Antivirais/sangue , Cavalos , Imunização/métodos , Imunização Secundária , Testes de Neutralização , Fatores de TempoRESUMO
With the use of the unified indirect solid-phase fluorescent enzyme immunoassay the combined evaluation of the antigenic and immunogenic properties of experimental whole-virion inactivated virus vaccines against Venezuelan and eastern equine encephalomyelitides, as well as of dried chemical typhus vaccine, has been made; their safety was determined indirectly by the content of ovalbumin. The protective role of antibodies evaluated by the solid-phase fluorescent enzyme immunoassay in typhus immunity has been shown.
Assuntos
Antígenos de Bactérias/análise , Antígenos Virais/análise , Vírus da Encefalite Equina do Leste/imunologia , Vírus da Encefalite Equina Venezuelana/imunologia , Fluorimunoensaio/métodos , Rickettsia prowazekii/imunologia , Vacinas Antirrickéttsia/análise , Vacinas Virais/análise , Animais , Anticorpos Antibacterianos/sangue , Anticorpos Antivirais/sangue , Epitopos/análise , Cobaias , Macaca fascicularis , Coelhos , Vacinas Antirrickéttsia/imunologia , Vacinas de Produtos Inativados/análise , Vacinas de Produtos Inativados/imunologia , Vacinas Virais/imunologiaRESUMO
A single and double immunization of Papio hamadryas monkeys with an inactivated concentrated purified adsorbed preparation of Lassa virus in doses of 0.5-1.0 and 1.1-2.1 mg by protein, respectively, protected the animals against the development of the disease and occurrence of viremia after an intramuscular challenge with 0.4 PFU dose. Upon aerogenic infection of monkeys protection of 50% of the animals was achieved by a single immunization with the inactivated preparation in a dose 1.1 mg by protein.
Assuntos
Vírus Lassa/imunologia , Vacinas Virais/isolamento & purificação , Animais , Anticorpos Antivirais/sangue , Testes de Fixação de Complemento , Relação Dose-Resposta Imunológica , Avaliação Pré-Clínica de Medicamentos , Imunização , Febre Lassa/imunologia , Febre Lassa/mortalidade , Febre Lassa/prevenção & controle , Vírus Lassa/isolamento & purificação , Testes de Neutralização , Papio , Fatores de Tempo , Vacinas de Produtos Inativados/imunologia , Vacinas de Produtos Inativados/isolamento & purificação , Vacinas Virais/imunologia , Cultura de VírusRESUMO
The data of the comparative study of the immunomodulating action of donor interferon and reaferon in experiments with typhus vaccine, used as an experimental model, on monkeys of different species and guinea pigs are presented. One of the main properties of interferons, besides their antiviral activity, is their capacity for producing an immunomodulating effect.
Assuntos
Adjuvantes Imunológicos/farmacologia , Interferon Tipo I/imunologia , Interferon-alfa/imunologia , Rickettsia prowazekii/imunologia , Vacinas Antirrickéttsia/imunologia , Animais , Formação de Anticorpos/efeitos dos fármacos , Avaliação Pré-Clínica de Medicamentos , Quimioterapia Combinada , Cobaias , Humanos , Imunização , Interferon alfa-2 , Macaca fascicularis , Macaca mulatta , Proteínas Recombinantes , Vacinas Sintéticas/imunologiaAssuntos
Ebolavirus , Vírus Lassa , Marburgvirus , Animais , Modelos Animais de Doenças , Ebolavirus/efeitos dos fármacos , Ebolavirus/patogenicidade , Febres Hemorrágicas Virais/diagnóstico , Febres Hemorrágicas Virais/tratamento farmacológico , Febres Hemorrágicas Virais/microbiologia , Humanos , Febre Lassa/diagnóstico , Febre Lassa/tratamento farmacológico , Febre Lassa/microbiologia , Vírus Lassa/efeitos dos fármacos , Vírus Lassa/patogenicidade , Doença do Vírus de Marburg/diagnóstico , Doença do Vírus de Marburg/tratamento farmacológico , Doença do Vírus de Marburg/microbiologia , Marburgvirus/efeitos dos fármacos , Marburgvirus/patogenicidade , PesquisaRESUMO
A comparative evaluation of the developed variants of indirect solid-phase enzyme immunoassay using fluorogenic and chromogenic substrates for the determination of Marburg virus antigen was carried out. The resolving capacity of this method was 3.8 x 10(-9) g of protein for the former and 3.1 x 10(-8) g of protein for the latter substrate. Cross titrations demonstrated the lack of common antigens with Ebola virus.
Assuntos
Antígenos Virais/análise , Marburgvirus/imunologia , Animais , Ebolavirus/imunologia , Estudos de Avaliação como Assunto , Himecromona/análogos & derivados , Técnicas Imunoenzimáticas , Indicadores e Reagentes , Nitrofenóis , Compostos Organofosforados , CoelhosRESUMO
A relationship between ribamydil concentration and the intensity of accumulation of peroxidation products was found while storing ribamydil-containing liposomes which indicated a significant pro-oxidant activity of ribamydil. Increasing lethality of guinea pigs and white mice infected with Venezuelan equine encephalomyelitis virus was shown while using liposomal ribamydil containing from 4.78 to 6.82 nmol. ml-1 malonic dialdehyde which appeared to be associated with disordered function of the antioxidant system of the experimental animals.
Assuntos
Encefalomielite Equina Venezuelana/tratamento farmacológico , Peroxidação de Lipídeos/efeitos dos fármacos , Ribavirina/farmacocinética , Febre do Vale de Rift/tratamento farmacológico , Animais , Relação Dose-Resposta a Droga , Portadores de Fármacos , Avaliação Pré-Clínica de Medicamentos , Encefalomielite Equina Venezuelana/metabolismo , Encefalomielite Equina Venezuelana/mortalidade , Radicais Livres , Cobaias , Lipossomos , Malondialdeído/metabolismo , Camundongos , Ratos , Ribavirina/administração & dosagem , Febre do Vale de Rift/metabolismo , Febre do Vale de Rift/mortalidadeRESUMO
The intratracheal introduction of R. burnetii is the method of infection, highly similar to aerogenic infection, the most widespread route of R. burnetii infection in humans under natural conditions. This suggests that the study of the pathogenesis of Q fever resulting from the intratracheal infection of guinea pigs is highly topical. The authors have established that after the penetration of the infective agent generalized infection develops in the animals with rickettsiae accumulating mostly in the lungs and the organs of the reticuloendothelial system being affected; this infection is commonly more pronounced than that developing after the intraperitoneal inoculation of rickettsiae.
Assuntos
Febre Q/etiologia , Animais , Coxiella burnetii/isolamento & purificação , Coxiella burnetii/patogenicidade , Modelos Animais de Doenças , Cobaias , Febre Q/microbiologia , Fatores de TempoRESUMO
Experiments in guinea pigs and M. rhesus monkeys showed the possibility of contact infection of animals with Marburg virus. Secondary infection occurred most intensively when the monkeys were kept together but was also shown to be possible when the animals were separated but placed in the direction of the air flow from the sick monkeys as well as by "nose-to-nose" contact excluding the alimentary mode of transmission and the role of the agent excreted in the urine.
Assuntos
Doença do Vírus de Marburg/transmissão , Aerossóis , Animais , Testes de Fixação de Complemento , Cobaias , Macaca mulatta , Doença do Vírus de Marburg/imunologia , Doença do Vírus de Marburg/microbiologia , Marburgvirus/isolamento & purificação , Inoculações Seriadas , Fatores de TempoAssuntos
Vírus da Encefalite Equina Venezuelana/imunologia , Vacinas Virais/imunologia , Animais , Cricetinae , Vírus da Encefalite Equina Venezuelana/patogenicidade , Encefalomielite Equina Venezuelana/patologia , Cobaias , Doenças dos Cavalos/patologia , Cavalos , Macaca mulatta , Mesocricetus , Camundongos , Coelhos , Vacinas Atenuadas/imunologia , Vacinas Atenuadas/isolamento & purificação , Vacinas Virais/isolamento & purificação , VirulênciaRESUMO
Intramuscular or aerogenic inoculation of baboons with Lassa virus reproduced a severe form of the disease which clinically, pathoanatomically and virologically resembles the severe form of human Lassa fever reported in the literature. The severity of monkey infection is demonstrated by rapid development of symptoms of general toxicity in the presence of fever, manifestations of hemorrhagic diathesis, high level of viremia and isolation of the causative agent from nasopharyngeal washings.
Assuntos
Febre Lassa/etiologia , Animais , Febre Lassa/microbiologia , Febre Lassa/patologia , Vírus Lassa/isolamento & purificação , Fígado/microbiologia , Fígado/patologia , Pulmão/microbiologia , Pulmão/patologia , Nasofaringe/microbiologia , Papio , Fatores de TempoRESUMO
The therapeutic efficacy of ribamydil and virasol was evaluated in experimental Lassa fever in monkeys which received these drugs at various intervals after the onset of the clinical illness. Ribamydil or virasol administered in the first day of fever protected from death 60% to 66% of the infected animals, but when the drugs were given 2 days after the onset of fever the number of survivors declined to 0.20%. When the treatment was started 4 days after the onset of fever none of the drugs prevented deaths of Lassa virus-infected baboons.