Mining, expression, and phylogenetic analysis of volatile terpenoid biosynthesis-related genes in different tissues of ten Elsholtzia species based on transcriptomic analysis.

We sequenced the leaf and inflorescence transcriptomes of 10 Elsholtzia species to mine genes related to the volatile terpenoid metabolic pathway. A total of 184.68 GB data and 1,231,162,678 clean reads were obtained from 20 Elsholtzia samples, and 333,848 unigenes with an average length of at least 1440 bp were obtained by Trinity assembly. KEGG pathway analysis showed that there were three pathways related to volatile terpene metabolism: terpenoid backbone biosynthesis (No. ko00900), monoterpenoid biosynthesis (No. ko00902), and sesquiterpenoid and triterpenoid biosynthesis (No. ko00909), with 437, 125, and 121 related unigenes, respectively. The essential oil content and composition in 20 Elsholtzia samples were determined by gas chromatography-mass spectrometry. The results showed that there were obvious interspecific differences among the 10 Elsholtzia species, but there were no significant differences between the different tissues among species. The expression levels of seven candidate genes involved in volatile terpenoid biosynthesis in Elsholtzia were further analyzed by quantitative real-time PCR. The results showed that HMGS had the highest expression among all genes, followed by GGPS4. In addition, there was not a significant correlation between the seven genes and the components with high essential oil contents. Combined with the essential oil components detected in this study, the possible biosynthetic pathway of the characteristic components in Elsholtzia plants was speculated to be a metabolic pathway with geraniol as the starting point and elsholtzione as the end product. Phylogenetic analysis was conducted using the nucleotide sequences of the geranyl diphosphate synthase candidate genes, and the results showed that genes related to the volatile terpenoid biosynthetic pathway may be more suitable gene fragments for resolving the Elsholtzia phylogeny.

Taxonomic studies on the genus Isotrema (Aristolochiaceae) from China III: I.pseudohei, a new species from Yunnan, Southwest China.

Isotremapseudohei, a new species from Yunnan, Southwest China, is described and illustrated. It is morphologically similar to I.hei and I.moupinense, but differs from the former in the colour of flower and throat, the size of throat and the shape of gynostemium lobes, and from the latter in the shape of lamina and gynostemium lobes.

Differentiation in fructification percentage between two morphs of Amomum tsaoko (Zingiberaceae).

Amomum tsaoko is a flexistylous ginger. Flexistyly is a unique floral mechanism promoting outcrossing, which is known only in some species of Zingiberaceae till date. This is a pioneer report on flexistyly in A. tsaoko from the aspect of fructification percentage to clarify its influence on reproduction. We observed in 2007 and 2008 that the fructification percentage of the anaflexistyled and the cataflexistyled inflorescence were 14.89 ± 10.35% and 11.31 ± 7.91% respectively, with significant difference (d.f. = 141.920, t = 2.518, P = 0.013 < 0.05). The greatly significant difference between 2007 and 2008 were present in both the flower number (d.f. = 93, t = -2.819, P = 0.006 < 0.01) and the fructification percentage (d.f. = 93, t = -2.894, P = 0.005 < 0.01) of the cataflexistylous inflorescence. Although the two morphs were similar in morphological characteristics, there was some gender differentiation between them, showing a possibility that the anaflexistylous morph might function more as females and the cataflexistylous morph more as males. Reproduction of the cataflexistylous morph was significantly sensitive to change of environmental factors, in contrast to the anaflexistylous morph, thus the yield varied between the abundant year (2008) and the off year (2007).

[Study on comparison of quantitative multistate character of infructescence of Amomum tsao-ko from five populations].

OBJECTIVE: To study the difference of the quantitative multistate character of infructescence of Amomum tsao-ko from five populations, and the correlations between the quantitative multistate character and altitude, longitude and latitude. METHODS: By observing and analyzing the quantitative multistate character of infructescence of Amomum tsao-ko from five populations. RESULTS: There is highly significant difference among the number of flowerlets each inflorescence from five populations. The higher the altitude, the less the number of flowerlets each inflorescence; and the higher the longitude is, the more the number of flowerlets each inflorescence. The temperature and the humidity of the circumstance affects the number of flowerlets each inflorescence. Lower temperature, higher diurnal variation of temperature, and higher diurnal variation of relative humidity causes less number of flowerlets each inflorescence. There is significantly negative correlation between the number of flowerlets each inflorescence and the maturing rate of infructescence. The difference of the maturing rate of infructescence from five populations don't approach significant level. The difference of the maturing rate of infructescence approaches significant level by multiple comparisons between Magnan population and Baoshan population, Gongshan population and Xichou population. There is not significant correlation between the maturing rate of infructescence and the altitude, the longitude and the latitude. CONCLUSION: The factors of the circumstance affects the number of flowerlets each inflorescence. The climate by the change of the altitude affects the number of flowerlets each inflorescence highly significantly. Selecting the fine genetic resources of Amomum tsao-ko for high yielding, the number of flowerlets of inflorescence is not better character. Maybe the difference of the maturing rate of inflorescence is mainly caused by different genetic resources. Analyzing the difference of genetic regenetic resources of Amomum tsao-ko.

[Study on quantitative multistate character of fruit and seed of Amomum tsaoko].

OBJECTIVE: To explore the regulation of the growth of fruit of Amomum tsaoko and the factors influencing the weight of fruit. METHODS: To compare and analyze the quantitative multistate character of fruit and seed of Amomum tsaoko. RESULTS: The quantitative multistate character of fruit and seed of Amomum tsaoko in populations and between populations were abundant. There was positive relativity between the rate of fertilization of ovule and the rate of fructify and this indicated the different heredity character among plants. The weight of fruit was mainly decided by the weight of seed regiment, The more the seed quantity, the larger the weight of seed regiment, and the larger the weight of fruit, then the higher the yield. The variety of the weight of fruit and the quantity of seed presented peak form along with the proper order of fruit growth in inflorescence but had no relativity. CONCLUSION: The abundant quantitative multistate character of fruit and seed of Amomum tsaoko provide the abundant materials for selecting good varieties. For high yield we should not only choose plants with high rate of fructify and high rate of fertilization of ovule the, but also pay attention to the influence of pollination to yield.