Parental bleaching susceptibility leads to differences in larval fluorescence and dispersal potential in Pocillopora acuta corals.

Coral reef ecosystems are declining at an alarming rate. Increasing seawater temperatures and occurrence of extreme warming events can impair sexual reproduction in reef-building corals and inhibit the ability for coral communities to replenish and persist. Here, we investigated the role of photophysiology on the reproductive ecology of Pocillopora acuta coral colonies by focusing on the impacts of bleaching susceptibility of parents on reproduction and larval performance, during an El Niño Southern Oscillation event in Mo'orea, French Polynesia. Elevated temperature conditions at that time induced bleaching phenotypic differences among P. acuta individuals: certain colonies became pale (from the loss of pigments and/or decline in symbiont cell density), while others remained pigmented (normal/high symbiont cell density). More specifically, we studied the impact of parental phenotypes on offspring's fluorescence by counting released larvae and sorting them by fluorescence types, we assessed survival to thermal stress, recruitment success and post-recruitment survival of released larvae from each fluorescent phenotype, during summer months (February to April 2016). Our results showed that red and green fluorescent larvae released by P. acuta had distinct physiological performances: red fluorescent larvae exhibited a higher survival into the pelagic phase regardless temperature conditions, with lower capacity to settle and survive post-recruitment, compared to green larvae that settle within a short period. Interestingly, pale colonies released two-to seven-fold more red fluorescent larvae than pigmented colonies did. In the light of our results, photophysiological profiles of the brooding P. acuta parental colonies may modulate the fluorescence features of released larvae, and thus influence the dispersal strategy of their offspring, the green fluorescent larval phenotypes being more performant in the benthic than pelagic phase.

Co-occurring nematodes and bacteria in submarine canyon sediments.

In submarine canyon sediments, bacteria and nematodes dominate the benthic biomass and play a key role in nutrient cycling and energy transfer. The diversity of these communities remains, however, poorly studied. This work aims at describing the composition of bacteria and nematode communities in the Lacaze-Duthiers submarine canyon in the north-western Mediterranean Sea. We targeted three sediment depths for two consecutive years and investigated the communities using nuclear markers (18S rRNA and 16S rRNA genes). High throughput sequencing combined to maximal information coefficient (MIC) statistical analysis allowed us to identify, for the first time, at the same small scale, the community structures and the co-occurrence of nematodes and bacteria Operational Taxonomic Units across the sediment cores. The associations detected by MIC revealed marked patterns of co-occurrences between the bacteria and nematodes in the sediment of the canyon and could be linked to the ecological requirements of individual bacteria and nematodes. For the bacterial community, Delta- and Gammaproteobacteria sequences were the most abundant, as seen in some canyons earlier, although Acidobacteria, Actinobacteria and Planctomycetes have been prevalent in other canyon sediments. The 20 identified nematode genera included bacteria feeders as Terschellingia, Eubostrichus, Geomonhystera, Desmoscolex and Leptolaimus. The present study provides new data on the diversity of bacterial and nematodes communities in the Lacaze-Duthiers canyon and further highlights the importance of small-scale sampling for an accurate vision of deep-sea communities.

Thermal resistances and acclimation potential during coral larval ontogeny in Acropora pulchra.

Rising temperature can adversely affect specific functions of corals. Coral gametes and planulae of Acropora pulchra were evaluated to determine their temperature resistances, and the potential of developmental thermal acclimation was examined on gametes. Results highlight that fertilization success displays a relatively high thermal resistance at ET50 (median effective temperature) 31.5 ±â€¯0.5 °C after 4 h and 30 min. Additionally, probability of larval survival is halved at LT50 (median lethal temperature) 28.4 ±â€¯0.42 °C after 14 days. The pre-exposure of oocytes to 30 °C and 32 °C for 1 h increases the cell development pace during fertilization at ambient temperature. Pre-exposure of gametes, separately at 32 °C for 1 h, increases fertilization success rate by 63% at 32°C, conversely, pre-exposure to 30 °C induces more variable results. These results evidenced the occurrence of developmental thermal acclimation as a result of thermal pre-exposure of oocytes.

Hyposalinity stress compromises the fertilization of gametes more than the survival of coral larvae.

The life cycle of coral is affected by natural and anthropogenic perturbations occurring in the marine environment. In the context of global changes, it is likely that rainfall events will be more intense and that coastal reefs will be exposed to sudden drops in salinity. Therefore, a better understanding of how corals-especially during the pelagic life stages-are able to deal with declines in salinity is crucial. To fill this knowledge gap, this work investigated how gametes and larva stages of two species of Acropora (Acropora cytherea and Acropora pulchra) from French Polynesia cope with drops in salinity. An analysis of collected results highlights that both Acropora coral gametes displayed the same resistance to salinity changes, with 4h30-ES50 (effective salinity that decrease by 50% the fertilization success after 4h30 exposure) of 26.6 ± 0.1 and 27.5 ± 0.3‰ for A. cytherea and A. pulchra, respectively. This study also revealed that coral gametes were more sensitive to decreases in salinity than larvae, for which significant changes are only observed at 26‰ for A. cytherea after 14 d of exposure. Although rising seawater temperatures and ocean acidification are often perceived as the main threat for the survival of coral reefs, our work indicates that 70% of the gametes could be killed during a single night of spawning by a rainfall event that decreases salinity to 26‰. This suggests that changes in the frequency and intensity of rainfall events associated with climate changes should be taken seriously in efforts to both preserve coral gametes and ensure the persistence and renewal of coral populations.

High resistance of Acropora coral gametes facing copper exposure.

Pollution by heavy metals remains today an important threat to the health of humans and ecosystems, but there is still a paucity of data on the response of early life stages of key organisms. In this context, the present work assessed the fertilization success rate of two Acropora species (A. cytherea and A. pulchra) from the French Polynesia reefs exposed to six increasing copper concentrations in seawater. The two species showed a relatively high tolerance to copper (4h30-EC50 was 69.4 ± 4.8 µg L(-1) and 75.4 ± 6.4 µg L(-1) for A. cytherea and A. pulchra, respectively). As Cu concentration increases, an increasing proportion of deformed embryos was recorded (67.6% and 58.5% for A. cytherea and A. pulchra, respectively, at 220 µg Cu L(-1)). These results demonstrated thus, that high levels of copper could negatively impair the normal fertilization process of coral gametes and therefore alter the renewal of coral populations. Since the two Acropora species investigated in this study displayed a high resistance to copper, these results should be considered in the context of multiple stressors associated with climate change, where rising temperature or ocean acidification may significantly exacerbate copper toxicity.