RESUMO
From mouse to primate, there is a striking discontinuity in our current understanding of the neural coding of motion direction. In non-primate mammals, directionally selective cell types and circuits are a signature feature of the retina, situated at the earliest stage of the visual process. In primates, by contrast, direction selectivity is a hallmark of motion processing areas in visual cortex, but has not been found in the retina, despite significant effort. Here we combined functional recordings of light-evoked responses and connectomic reconstruction to identify diverse direction-selective cell types in the macaque monkey retina with distinctive physiological properties and synaptic motifs. This circuitry includes an ON-OFF ganglion cell type, a spiking, ON-OFF polyaxonal amacrine cell and the starburst amacrine cell, all of which show direction selectivity. Moreover, we discovered that macaque starburst cells possess a strong, non-GABAergic, antagonistic surround mediated by input from excitatory bipolar cells that is critical for the generation of radial motion sensitivity in these cells. Our findings open a door to investigation of a precortical circuitry that computes motion direction in the primate visual system.
Assuntos
Conectoma , Macaca , Retina , Células Amácrinas/fisiologia , Animais , Potenciais Evocados Visuais/fisiologia , Macaca/fisiologia , Mamíferos , Camundongos , Primatas/fisiologia , Retina/fisiologia , Células Ganglionares da Retina/fisiologia , Sinapses/fisiologiaRESUMO
In primates, broad thorny retinal ganglion cells are highly sensitive to small, moving stimuli. They have tortuous, fine dendrites with many short, spine-like branches that occupy three contiguous strata in the middle of the inner plexiform layer. The neural circuits that generate their responses to moving stimuli are not well-understood, and that was the goal of this study. A connectome from central macaque retina was generated by serial block-face scanning electron microscopy, a broad thorny cell was reconstructed, and its synaptic inputs were analyzed. It received fewer than 2% of its inputs from both ON and OFF types of bipolar cells; the vast majority of its inputs were from amacrine cells. The presynaptic amacrine cells were reconstructed, and seven types were identified based on their characteristic morphology. Two types of narrow-field cells, knotty bistratified Type 1 and wavy multistratified Type 2, were identified. Two types of medium-field amacrine cells, ON starburst and spiny, were also presynaptic to the broad thorny cell. Three types of wide-field amacrine cells, wiry Type 2, stellate wavy, and semilunar Type 2, also made synapses onto the broad thorny cell. Physiological experiments using a macaque retinal preparation in vitro confirmed that broad thorny cells received robust excitatory input from both the ON and the OFF pathways. Given the paucity of bipolar cell inputs, it is likely that amacrine cells provided much of the excitatory input, in addition to inhibitory input.
Assuntos
Células Amácrinas/fisiologia , Conectoma/métodos , Retina/citologia , Retina/fisiologia , Células Ganglionares da Retina/fisiologia , Sinapses/fisiologia , Células Amácrinas/ultraestrutura , Animais , Macaca , Macaca nemestrina , Masculino , Retina/ultraestrutura , Células Ganglionares da Retina/ultraestrutura , Sinapses/ultraestruturaRESUMO
Electrical coupling has been reported to occur only between homotypic retinal ganglion cells, in line with the concept of parallel processing in the early visual system. Here, however, we show reciprocal correlated firing between heterotypic ganglion cells in multielectrode array recordings during light stimulation in retinas of adult guinea pigs of either sex. Heterotypic coupling was further confirmed via tracer spread after intracellular injections of single cells with neurobiotin. Both electrically coupled cell types were sustained ON center ganglion cells but showed distinct light response properties and receptive field sizes. We identified one of the involved cell types as sustained ON α-ganglion cells. The presence of electrical coupling between heterotypic ganglion cells introduces a network motif in which the signals of distinct ganglion cell types are partially mixed at the output stage of the retina.SIGNIFICANCE STATEMENT The visual information is split into parallel pathways, before it is sent to the brain via the output neurons of the retina, the ganglion cells. Ganglion cells can form electrical synapses between dendrites of neighboring cells in support of lateral information exchange. To date, ganglion-to-ganglion cell coupling is thought to occur only between cells of the same type. Here, however, we show that electrical coupling between different types of ganglion cells exists in the mammalian retina. We provide functional and anatomical evidence that two different types of ganglion cells share information via electrical coupling. This new network motif extends the impact of the heavily studied coding benefits of homotypic coupling to heterotypic coupling across parallel neuronal pathways.
Assuntos
Sinapses Elétricas/fisiologia , Células Ganglionares da Retina/citologia , Células Ganglionares da Retina/fisiologia , Transmissão Sináptica/fisiologia , Animais , Feminino , Cobaias , MasculinoRESUMO
In the mammalian retina, horizontal cells receive glutamatergic inputs from many rod and cone photoreceptors and return feedback signals to them, thereby changing photoreceptor glutamate release in a light-dependent manner. Horizontal cells also provide feedforward signals to bipolar cells. It is unclear, however, how horizontal cell signals also affect the temporal, spatial, and contrast tuning in retinal output neurons, the ganglion cells. To study this, we generated a genetically modified mouse line in which we eliminated the light dependency of feedback by deleting glutamate receptors from mouse horizontal cells. This genetic modification allowed us to investigate the impact of horizontal cells on ganglion cell signaling independent of the actual mode of feedback in the outer retina and without pharmacological manipulation of signal transmission. In control and genetically modified mice (both sexes), we recorded the light responses of transient OFF-α retinal ganglion cells in the intact retina. Excitatory postsynaptic currents (EPSCs) were reduced and the cells were tuned to lower temporal frequencies and higher contrasts, presumably because photoreceptor output was attenuated. Moreover, receptive fields of recorded cells showed a significantly altered surround structure. Our data thus suggest that horizontal cells are responsible for adjusting the dynamic range of retinal ganglion cells and, together with amacrine cells, contribute to the center/surround organization of ganglion cell receptive fields in the mouse.SIGNIFICANCE STATEMENT Horizontal cells represent a major neuronal class in the mammalian retina and provide lateral feedback and feedforward signals to photoreceptors and bipolar cells, respectively. The mode of signal transmission remains controversial and, moreover, the contribution of horizontal cells to visual processing is still elusive. To address the question of how horizontal cells affect retinal output signals, we recorded the light responses of transient OFF-α retinal ganglion cells in a newly generated mouse line. In this mouse line, horizontal cell signals were no longer modulated by light. With light response recordings, we show that horizontal cells increase the dynamic range of retinal ganglion cells for contrast and temporal changes and contribute to the center/surround organization of their receptive fields.
Assuntos
Glutamina/metabolismo , Células Ganglionares da Retina/metabolismo , Células Horizontais da Retina/metabolismo , Transmissão Sináptica/fisiologia , Animais , Feminino , Masculino , Camundongos , Camundongos TransgênicosRESUMO
Retinal OFF bipolar cells show distinct connectivity patterns with photoreceptors in the wild-type mouse retina. Some types are cone-specific while others penetrate further through the outer plexiform layer (OPL) to contact rods in addition to cones. To explore dendritic stratification of OFF bipolar cells in the absence of rods, we made use of the 'cone-full' Nrl-/- mouse retina in which all photoreceptor precursor cells commit to a cone fate including those which would have become rods in wild-type retinas. The dendritic distribution of OFF bipolar cell types was investigated by confocal and electron microscopic imaging of immunolabeled tissue sections. The cells' dendrites formed basal contacts with cone terminals and expressed the corresponding glutamate receptor subunits at those sites, indicating putative synapses. All of the four analyzed cell populations showed distinctive patterns of vertical dendritic invasion through the OPL. This disparate behavior of dendritic extension in an environment containing only cone terminals demonstrates type-dependent specificity for dendritic outgrowth in OFF bipolar cells: rod terminals are not required for inducing dendritic extension into distal areas of the OPL.
Assuntos
Fatores de Transcrição de Zíper de Leucina Básica/genética , Células Dendríticas/ultraestrutura , Proteínas do Olho/genética , Células Bipolares da Retina/ultraestrutura , Células Fotorreceptoras Retinianas Bastonetes/ultraestrutura , Sinapses/ultraestrutura , Animais , Células Dendríticas/metabolismo , Modelos Animais de Doenças , Ácido Glutâmico/genética , Ácido Glutâmico/metabolismo , Humanos , Camundongos , Camundongos Knockout , Microscopia Eletrônica , Células Bipolares da Retina/metabolismo , Segmento Externo das Células Fotorreceptoras da Retina/ultraestrutura , Células Fotorreceptoras Retinianas Bastonetes/metabolismo , Sinapses/genética , Sinapses/metabolismoRESUMO
At early stages of visual processing, receptive fields are typically described as subtending local regions of space and thus performing computations on a narrow spatial scale. Nevertheless, stimulation well outside of the classical receptive field can exert clear and significant effects on visual processing. Given the distances over which they occur, the retinal mechanisms responsible for these long-range effects would certainly require signal propagation via active membrane properties. Here the physiology of a wide-field amacrine cell-the wiry cell-in macaque monkey retina is explored, revealing receptive fields that represent a striking departure from the classic structure. A single wiry cell integrates signals over wide regions of retina, 5-10 times larger than the classic receptive fields of most retinal ganglion cells. Wiry cells integrate signals over space much more effectively than predicted from passive signal propagation, and spatial integration is strongly attenuated during blockade of NMDA spikes but integration is insensitive to blockade of NaV channels with TTX. Thus these cells appear well suited for contributing to the long-range interactions of visual signals that characterize many aspects of visual perception.
Assuntos
Células Amácrinas/fisiologia , Transmissão Sináptica , Campos Visuais , Células Amácrinas/metabolismo , Animais , Feminino , Macaca , Masculino , N-Metilaspartato/metabolismo , Células Ganglionares da Retina/metabolismo , Células Ganglionares da Retina/fisiologia , Canais de Sódio/metabolismoRESUMO
Functional analyses exist only for a few of the morphologically described primate ganglion cell types, and their correlates in other mammalian species remain elusive. Here, we recorded light responses of broad thorny cells in the whole-mounted macaque retina. They showed ON-OFF-center light responses that were strongly suppressed by stimulation of the receptive field surround. Spike responses were delayed compared with parasol ganglion cells and other ON-OFF cells, including recursive bistratified ganglion cells and A1 amacrine cells. The receptive field structure was shaped by direct excitatory synaptic input and strong presynaptic and postsynaptic inhibition in both ON and OFF pathways. The cells responded strongly to dark or bright stimuli moving either in or out of the receptive field, independent of the direction of motion. However, they did not show a maintained spike response either to a uniform background or to a drifting plaid pattern. These properties could be ideally suited for guiding movements involved in visual pursuit. The functional characteristics reported here permit the first direct cross-species comparison of putative homologous ganglion cell types. Based on morphological similarities, broad thorny ganglion cells have been proposed to be homologs of rabbit local edge detector ganglion cells, but we now show that the two cells have quite distinct physiological properties. Thus, our data argue against broad thorny cells as the homologs of local edge detector cells.
Assuntos
Percepção de Movimento/fisiologia , Acompanhamento Ocular Uniforme/fisiologia , Retina/fisiologia , Células Ganglionares da Retina/citologia , Células Ganglionares da Retina/fisiologia , Potenciais de Ação/fisiologia , Células Amácrinas/fisiologia , Animais , Feminino , Macaca , Masculino , Estimulação Luminosa , Retina/anatomia & histologia , Campos Visuais/fisiologia , Vias Visuais/fisiologiaRESUMO
The distribution of the soluble NSF-attachment protein receptor protein syntaxin-4 and the Na-K-Cl cotransporter (NKCC) were investigated in the outer plexiform layer of human retina using immunohistochemistry. Both proteins, which are proposed to be components of a gamma-aminobutyric acid mediated feed-forward circuit from horizontal cells directly to bipolar cells, were enriched beneath S-cones. The expression pattern of syntaxin-4 was further analyzed in baboon and marmoset to determine if the synaptic specialization is common to primates. Syntaxin-4 was enriched beneath S-cones in both species, which together with the human results indicates that this specialization may have evolved for the purpose of mediating unique color vision capacities that are exclusive to primates.
Assuntos
Callithrix/fisiologia , Papio/fisiologia , Células Fotorreceptoras Retinianas Cones/citologia , Transdução de Sinais , Sinapses/metabolismo , Idoso , Animais , Percepção de Cores/fisiologia , Visão de Cores/fisiologia , Evolução Molecular , Feminino , Regulação da Expressão Gênica , Humanos , Proteínas Qa-SNARE/metabolismo , Células Fotorreceptoras Retinianas Cones/metabolismo , Especificidade da EspécieRESUMO
The functional roles and synaptic features of horizontal cells in the mammalian retina are still controversial. Evidence exists for feedback signaling from horizontal cells to cones and feed-forward signaling from horizontal cells to bipolar cells, but the details of the latter remain elusive. Here, immunohistochemistry and confocal microscopy were used to analyze the expression patterns of the SNARE protein syntaxin-4, the GABA receptor subunits α1 and ρ, and the cation-chloride cotransporters NKCC and KCC2 in the outer plexiform layer of primate retina. In macaque retina, as observed previously in other species, syntaxin-4 was expressed on dendrites and axon terminals of horizontal cells at cone pedicles and rod spherules. At cones, syntaxin-4 appeared densely clustered in two bands, at horizontal cell dendritic tips and at the level of desmosome-like junctions. Interestingly, in the lower band where horizontal cells may synapse directly onto bipolar cells, syntaxin-4 was highly enriched beneath short-wavelength sensitive (S) cones and colocalized with calbindin, a marker for HII horizontal cells. The enrichment at S-cones was not observed in either mouse or ground squirrel. Furthermore, high amounts of both GABA receptor and cation-chloride cotransporter subunits were found beneath primate S-cones. Finally, while syntaxin-4 was expressed by both HI and HII horizontal cell types, the intense clustering and colocalization with calbindin at S-cones indicated an enhanced expression in HII cells. Taken together, GABA receptors beneath cone pedicles, chloride transporters, and syntaxin-4 are putative constituents of a synaptic set of proteins which would be required for a GABA-mediated feed-forward pathway via horizontal cells carrying signals directly from cones to bipolar cells.
Assuntos
Retroalimentação Fisiológica/fisiologia , Células Fotorreceptoras Retinianas Cones/metabolismo , Células Horizontais da Retina/fisiologia , Transdução de Sinais/fisiologia , Sinapses/fisiologia , Animais , Imuno-Histoquímica , Macaca mulatta , Camundongos , Microscopia Confocal , Subunidades Proteicas/metabolismo , Proteínas Qa-SNARE/metabolismo , Receptores de GABA/metabolismo , Sciuridae , Membro 2 da Família 12 de Carreador de Soluto/metabolismo , Simportadores/metabolismo , Cotransportadores de K e Cl-RESUMO
Inhibitory glycine receptors (GlyRs) are composed of homologous α- (α1-4) and ß-subunits. The ß-subunits (GlyRß) interact via their large cytosolic loops with the postsynaptic scaffolding protein gephyrin and are therefore considered essential for synaptic localization. In situ hybridization studies indicate a widespread distribution of GlyRß transcripts throughout the mammalian central nervous system (CNS), whereas GlyRα mRNAs and proteins display more restricted expression patterns. Here we report the generation of a monoclonal antibody that specifically recognizes rodent GlyRß (mAb-GlyRß) and does not exhibit crossreactivity with any of the GlyRα1-4 subunits. Immunostaining with this antibody revealed high densities of punctate GlyRß immunoreactivity at inhibitory synapses in mouse spinal cord, brainstem, midbrain, and olfactory bulb but not in the neocortex, cerebellum, or hippocampus. This contrasts the abundance of GlyRß transcripts in all major regions of the rodent brain and suggests that GlyRß protein levels are regulated posttranscriptionally. When mAb-GlyRß was used in double-labeling experiments with GlyRα1-, α2-, α3-, or α4-specific antibodies to examine the colocalization of GlyRß with these GlyR subunits in the mouse retina, >90% of the GlyRα1-3 clusters detected were found to be GlyRß-immunoreactive. A subset (about 50%) of the GlyRα4 puncta in the inner plexiform layer, however, was found to lack GlyRß and gephyrin immunostaining. These GlyRα4-only clusters were apposed to bassoon immunoreactivity and hence synaptically localized. Their existence points to a gephyrin-independent synaptic localization mechanism for a minor subset of GlyRs.
Assuntos
Anticorpos Monoclonais , Química Encefálica , Encéfalo/metabolismo , Receptores de Glicina/análise , Medula Espinal/metabolismo , Animais , Anticorpos Monoclonais/imunologia , Especificidade de Anticorpos , Humanos , Imuno-Histoquímica , Camundongos , Camundongos Endogâmicos C57BL , Microscopia Confocal , Ratos , Receptores de Glicina/biossíntese , Medula Espinal/química , Sinapses/química , Sinapses/metabolismoRESUMO
Like most mammals, mice feature dichromatic color vision based on short (S) and middle (M) wavelength-sensitive cone types. It is thought that mammals share a retinal circuit that in dichromats compares S- and M-cone output to generate blue/green opponent signals, with bipolar cells (BCs) providing separate chromatic channels. Although S-cone-selective ON-BCs (type 9 in mouse) have been anatomically identified, little is known about their counterparts, the M-cone-selective OFF-BCs. Here, we characterized cone connectivity and light responses of selected mouse BC types using immunohistochemistry and electrophysiology. Our anatomical data indicate that four (types 2, 3a/b, and 4) of the five mouse OFF-BCs indiscriminately contact both cone types, whereas type 1 BCs avoid S-cones. Light responses showed that the chromatic tuning of the BCs strongly depended on their position along the dorsoventral axis because of the coexpression gradient of M- and S-opsin found in mice. In dorsal retina, where coexpression is low, most type 2 cells were green biased, with a fraction of cells (≈ 14%) displaying strongly blue-biased responses, likely reflecting S-cone input. Type 1 cells were also green biased but did not comprise blue-biased "outliers," consistent with type 1 BCs avoiding S-cones. We therefore suggest that type 1 represents the green OFF pathway in mouse. In addition, we confirmed that type 9 BCs display blue-ON responses. In ventral retina, all BC types studied here displayed similar blue-biased responses, suggesting that color vision is hampered in ventral retina. In conclusion, our data support an antagonistically organized blue/green circuit as the common basis for mammalian dichromatic color vision.
Assuntos
Visão de Cores/fisiologia , Retina/citologia , Células Bipolares da Retina/fisiologia , Células Fotorreceptoras Retinianas Cones/fisiologia , Vias Visuais/fisiologia , Potenciais de Ação/fisiologia , Animais , Proteínas de Transporte/genética , Cor , Percepção de Cores/fisiologia , Proteínas Ativadoras de GTPase/genética , Proteínas de Fluorescência Verde/genética , Luz , Camundongos , Camundongos Transgênicos , Microscopia Confocal , Modelos Estatísticos , Opsinas/metabolismo , Compostos Organofosforados/metabolismo , Aglutinina de Amendoim/metabolismo , Fenóis/metabolismo , Estimulação Luminosa/métodos , Receptores Imunológicos/genética , Receptores da Neurocinina-3/genética , Células Bipolares da Retina/classificação , Células Fotorreceptoras Retinianas Cones/classificaçãoRESUMO
Parallel processing of an image projected onto the retina starts at the first synapse, the cone pedicle, and each cone feeds its light signal into a minimum of eight different bipolar cell types. Hence, the morphological classification of bipolar cells is a prerequisite for analyzing retinal circuitry. Here we applied common bipolar cell markers to the cone-dominated ground squirrel retina, studied the labeling by confocal microscopy and electron microscopy, and compared the resulting bipolar cell types with those of the mouse (rod dominated) and primate retina. Eight different cone bipolar cell types (three OFF and five ON) and one rod bipolar cell were distinguished. The major criteria for classifying the cells were their immunocytochemical identity, their dendritic branching pattern, and the shape and stratification level of their axons in the inner plexiform layer (IPL). Immunostaining with antibodies against Gγ13, a marker for ON bipolar cells, made it possible to separate OFF and ON bipolars. Recoverin-positive OFF bipolar cells partly overlapped with ON bipolar axon terminals at the ON/OFF border of the IPL. Antibodies against HCN4 labeled the S-cone selective (bb) bipolar cell. The calcium-binding protein CaB5 was expressed in two OFF and two ON cone bipolar cell types, and CD15 labeled a widefield ON cone bipolar cell comparable to the DB6 in primate.
Assuntos
Retina/citologia , Células Bipolares da Retina/ultraestrutura , Sciuridae/anatomia & histologia , Animais , Biomarcadores/metabolismo , Canais de Cátion Regulados por Nucleotídeos Cíclicos/metabolismo , Humanos , Imuno-Histoquímica/métodos , Antígenos CD15/metabolismo , Camundongos , Microscopia Confocal , Células Bipolares da Retina/classificação , Células Bipolares da Retina/metabolismo , Vias Visuais/anatomia & histologiaRESUMO
Color vision in mammals is based on the expression of at least two cone opsins that are sensitive to different wavelengths of light. Furthermore, retinal pathways conveying color-opponent signals are required for color discrimination. Most of the primates are trichromats, and "color-coded channels" of their retinas are unveiled to a large extent. In contrast, knowledge of cone-selective pathways in nonprimate dichromats is only slowly emerging, although retinas of dichromats like mice or rats are extensively studied as model systems for retinal information processing. Here, we review recent progress of research on color-coded pathways in nonprimate dichromats to identify differences or similarities between di- and trichromatic mammals. In addition, we applied immunohistochemical methods and confocal microscopy to retinas of different species and present data on their neuronal properties, which are expected to contribute to color vision. Basic neuronal features such as the "blue cone bipolar cell" exist in every species investigated so far. Moreover, there is increasing evidence for chromatic OFF channels in dichromats and retinal ganglion cells that relay color-opponent signals to the brain. In conclusion, di- and trichromats share similar retinal pathways for color transmission and processing.
Assuntos
Visão de Cores/fisiologia , Células Bipolares da Retina/fisiologia , Vias Visuais/fisiologia , Animais , Callithrix , Humanos , Macaca , Camundongos , Microscopia Confocal , Ratos , Células Fotorreceptoras Retinianas Cones/citologia , Células Fotorreceptoras Retinianas Cones/fisiologia , Células Ganglionares da Retina/fisiologia , Células Horizontais da Retina/fisiologiaRESUMO
Protocadherins (Pcdhs) are thought to be key features of cell-type-specific synapse formation. Here we analyzed the expression pattern of Pcdh subunit ß16 (ß16) in the primate retina by applying antibodies against ß16, different subunits of ionotropic glutamate receptors (GluRs), and cell-type-specific markers as well as by coimmunoprecipitation and Western blots. Immunocytochemical localization was analyzed by confocal microscopy and preembedding electron microscopy. In the outer plexiform layer (OPL) H1, but not H2, horizontal cells expressed ß16 as revealed by the strong reduction of ß16 at short-wavelength-sensitive cones. ß16 colocalized with the GluR subunits GluR2-4 at horizontal cell dendritic tips and with GluR2-4 and GluR6/7 at the desmosome-like junctions. At the latter, these AMPA and kainate receptor subunits were found to be clustered within single synaptic hot spots. Additionally, ß16-labeled dendritic tips of OFF cone bipolar cells appeared in triad-associated positions at the cone pedicle base, pointing to ß16 expression by OFF midget or DB3 bipolar cells. In the inner plexiform layer, ß16 was localized also postsynaptically at most of the glutamatergic synapses. Overall, we provide evidence for a cell-type-specific localization of ß16 together with GluRs at defined postsynaptic sites and a coexistence of AMPA and kainate receptors within single synaptic hot spots. This study supports the hypothesis that ß16 plays an important role in the formation and/or stabilization of specific glutamatergic synapses, whereas our in vivo protein biochemical results argue against the existence of protein complexes formed by ß16 and GluRs.
Assuntos
Caderinas/metabolismo , Macaca , Neuropeptídeos/metabolismo , Receptores de AMPA/metabolismo , Receptores de Ácido Caínico/metabolismo , Retina/citologia , Sinapses/metabolismo , Animais , Biomarcadores/metabolismo , Humanos , Camundongos , Camundongos Endogâmicos C57BL , Microscopia Imunoeletrônica/métodos , Complexos Multiproteicos/metabolismo , Complexos Multiproteicos/ultraestrutura , Subunidades Proteicas/metabolismo , Receptores Ionotrópicos de Glutamato/metabolismo , Retina/metabolismo , Sinapses/ultraestruturaRESUMO
Information processing in the retina starts at the first synaptic layer, where photoreceptors and second-order neurons exhibit a complex architecture of glutamatergic and electrical synapses. To investigate the composition of this highly organized synaptic network, we determined the spatial relationship of zonula occludens-1 (ZO-1) with different connexins (Cx) and glutamate receptor (GluR) subunits in the outer plexiform layer (OPL) of rabbit, mouse, and monkey retinas. ZO-1 is well known as an intracellular component of tight and adherens junctions, but also interacts with various connexins at gap junctions. We found ZO-1 closely associated with Cx50 on dendrites of A-type horizontal cells in rabbit, and with Cx57 at dendro-dendritic gap junctions of mouse horizontal cells. The spatial arrangement of ZO-1 at the giant gap-junctional plaques in rabbit was particularly striking. ZO-1 formed a clear margin around the large Cx50 plaques instead of being colocalized with the connexin staining. Our finding suggests the involvement of ZO-1 in the composition of tight or adherens junctions around gap-junctional plaques instead of interacting with connexins directly. Furthermore, gap junctions were found to be clustered in close proximity to GluRs at the level of desmosome-like junctions, where horizontal cell dendrites converge before invaginating the cone pedicle. Based on this distinct spatial organization of gap junctions and GluRs, it is tempting to speculate that glutamate released from the photoreceptors may play a role in modulating the conductance of electrical synapses in the OPL.
Assuntos
Junções Comunicantes/ultraestrutura , Proteínas de Membrana/análise , Fosfoproteínas/análise , Retina/química , Retina/citologia , Células Horizontais da Retina/química , Células Horizontais da Retina/ultraestrutura , Junções Aderentes/ultraestrutura , Animais , Conexinas/análise , Conexinas/metabolismo , Dendritos/ultraestrutura , Desmossomos/fisiologia , Proteínas do Olho/metabolismo , Macaca fascicularis , Camundongos , Camundongos Endogâmicos C57BL , Coelhos , Receptores de Glutamato/análise , Junções Íntimas/ultraestrutura , Proteína da Zônula de Oclusão-1RESUMO
We report a quantitative analysis of the different bipolar cell types of the mouse retina. They were identified in wild-type mice by specific antibodies or in transgenic mouse lines by specific expression of green fluorescent protein or Clomeleon. The bipolar cell densities, their cone contacts, their dendritic coverage, and their axonal tiling were measured in retinal whole mounts. The results show that each and all cones are contacted by at least one member of any given type of bipolar cell (not considering genuine blue cones). Consequently, each cone feeds its light signals into a minimum of 10 different bipolar cells. Parallel processing of an image projected onto the retina, therefore, starts at the first synapse of the retina, the cone pedicle. The quantitative analysis suggests that our proposed catalog of 11 cone bipolar cells and one rod bipolar cell is complete, and all major bipolar cell types of the mouse retina appear to have been discovered.
Assuntos
Retina/citologia , Células Bipolares da Retina , Células Fotorreceptoras Retinianas Cones/citologia , Sinapses/fisiologia , Animais , Subunidade RIIbeta da Proteína Quinase Dependente de AMP Cíclico/metabolismo , Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , Canais de Cátion Regulados por Nucleotídeos Cíclicos/metabolismo , Dendritos/fisiologia , Proteínas de Fluorescência Verde/genética , Canais Disparados por Nucleotídeos Cíclicos Ativados por Hiperpolarização , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Receptores Imunológicos/genética , Receptores de Ácido Caínico/metabolismo , Receptores da Neurocinina-3/metabolismo , Receptores 5-HT3 de Serotonina/genética , Células Bipolares da Retina/classificação , Células Bipolares da Retina/citologia , Células Bipolares da Retina/fisiologia , Sinaptotagmina II/metabolismoRESUMO
Recent studies suggested that different types of OFF bipolar cells express specific types of ionotropic (AMPA or kainate) glutamate receptors (GluRs) at their contacts with cone pedicles. However, the question of which GluR type is expressed by which type of OFF bipolar cell in primate retina is still open. In this study, the expression of AMPA and kainate receptor subunits at the dendritic tips of flat (OFF) midget bipolar (FMB) cells was analyzed in the retina of the common marmoset, Callithrix jacchus. We used preembedding electron microscopy and double immunofluorescence with subunit-specific antibodies. The FMB cells were labeled with antibodies against the carbohydrate epitope CD15. Cone pedicles were identified with peanut agglutinin. Immunoreactivity for the GluR1 subunit and for CD15 is preferentially located at triad-associated flat contacts. Furthermore, the large majority of GluR1 immunoreactive puncta is localized at the dendritic tips of FMB cells. These results suggest that FMB cells express the AMPA receptor subunit GluR1. In contrast, the kainate receptor subunit GluR5 is not colocalized with the dendritic tips of FMB cells or with the GluR1 subunit. Immunoreactive puncta for the GluR1 subunit are found at all M/L-cone pedicles but are only rarely associated with S-cone pedicles. This is consistent with our recent findings in marmoset retina that FMB cells do not contact S-cone pedicles. The presence of GluR5 clusters at S-cone pedicles indicates that in primate retinas OFF bipolar cells expressing kainate receptor subunits receive some S-cone input.