RESUMO
OBJECTIVES: In this work, we evaluated the expression of microRNA-384 (miR-384) in human prostate cancer (CAP) cells and its regulatory role on CAP in vitro and in vivo functions. METHODS: In CAP cell lines, miR-384 expression was evaluated by qRT-PCR. In LNCaP and VCaP cells, lentiviral infection was done to overexpress miR-384. The regulatory effects of miR-384 overexpression on CAP in vitro proliferation and migration, and in vivo tumorigenesis, were evaluated, respectively. The targeting of miR-384 on Homeobox b7 gene (HOXB7), was evaluated by dual-luciferase reporter assay and qRT-PCR. In addition, HOXB7 was upregulated in miR-384-overexpressed CAP cells to evaluate the correlated role of HOXB7 in miR-384-mediated CAP proliferation and migration in vitro. RESULTS: MiR-384 was lowly expressed in CAP cell lines. Lentiviral infection induced miR-384 overexpression inhibited CAP in vitro proliferation and migration, and in vivo tumorigenesis. HOXB7 was directly targeted by miR-384 in CAP cells. In miR-384-overexpressed CAP cells, HOXB7 upregulation reversed the effect of miR-384 by promoting CAP in vitro proliferation and migration. CONCLUSION: MiR-384 was downregulated in CAP cells. MiR-384 overexpression suppressed CAP in vitro and in vivo development, possibly via acting through its downstream target gene of HOXB7.
Assuntos
Proteínas de Homeodomínio/genética , MicroRNAs/genética , Neoplasias da Próstata/genética , Carcinogênese/genética , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Regulação para Baixo/genética , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Masculino , Regulação para Cima/genéticaRESUMO
Medical device going home is an inevitable trend, however, using these devices has potential safety risks. Through introducing the home use electronic medical device products and related medical device standards, this paper provides recommendations on construction of standard system for home use electronic medical devices, to improve the advancement of existing medical device standard system and guide future medical standardization work, to fully utilize standars's guiding and security role in the scientific and technological innovation, industrial development.
Assuntos
Eletrônica Médica , Segurança de Equipamentos , Equipamentos e Provisões , Serviços de Assistência Domiciliar , Padrões de ReferênciaRESUMO
This article analyzes the new demand of emergency medical equipment in the current development trend based on the analysis of the development and current situation of emergency medicine in our country. At the same time it introduces the current industrial characteristics of our country. Finally it analyzes the development trend of this kind of equipment in the new emergency medicine field.
Assuntos
Serviços Médicos de Emergência , Equipamentos e Provisões , China , Medicina de EmergênciaRESUMO
This paper discusses the test method of auditory alarm in YY0709-2009 by analyzing the test requirement and process. Consider of the acoustic theory and the problem during the test, it provides some suggestions for improvement.
Assuntos
Alarmes Clínicos , Falha de Equipamento , Segurança de Equipamentos/métodos , AcústicaRESUMO
This paper introduces the meaning of YY0709-2009's issue and its scope. It emphasizes the details of visual and audible alarms. Reasonable suggestions to comply with these requirements are provided at the end of the article. It will be of help to understand YY0709-2009 and to manufacture or test the product under YY0709-2009.