Assessing the suitability of municipal sewage sludge and coconut bran as breeding medium for Oryza sativa L. seedlings and developing a standardized substrate.

The utilization of organic solid waste (OSW) for preparing standardized seedling substrates is a main challenge due to its temporal and spatial variability. This study aims to form models based on data from the literature and validate them through experiments to explore a standardized seedling substrate. The typical OSW in Hainan Province, including municipal sewage sludge (MSS), coconut bran (CB), seaweed mud (SM), and municipal sewage sludge biochar (MSSB), was used as raw material. A series of six mixing ratios was tested, namely: T1 (0% MSS: 90% CB), T2 (10% MSS: 80% CB), T3 (30% MSS: 60% CB), T4 (50% MSS: 40% CB), T5 (70% MSS: 20% CB), and T6 (90% MSS: 0% CB). SM and MSSB were added as amendment materials at 5% (w/w) for each treatment. The physicochemical properties of substrates, agronomic traits of rice seedlings and microbial diversity were analyzed. The results showed that the four kinds of OSW played an active role in providing rich sources of nutrients. The dry weight of the above-ground part was 2.98 times greater in T3 than that of the commercial substrate. Furthermore, the microbial analysis showed a higher abundance of Actinobacteria in T3, representing the stability of the composted products. Finally, the successful fitting of the results with the linear regression models could establish relationship equations between the physicochemical properties of the substrate and the growth characteristics of seedlings. The relevant parameters suitable for the growth of rice seedlings were as follows: pH (6.46-7.01), EC (less than 2.12 mS cm-1), DD (0.13-0.16 g cm-3), and TPS (65.68-82.73%). This study proposed relevant parameters and models for standardization of seedling substrate, which would contribute to ensuring the quality of seedlings and OSW resource utilization.

Ecological circular agriculture: A case study evaluating biogas slurry applied to rice in two soils.

In the context of carbon peak, neutrality, and circular agricultural economy, the use of renewable resources from agricultural processing for plant cultivation still needs to be explored to clarify material flow and its ecological effects. Paddy-upland rotation is an effective agricultural strategy to improve soil quality. This study evaluated the effects of biogas slurry application against those of chemical fertilisers in these two typical Chinese cropping soils. The application of biogas slurry increased total carbon content in paddy soil by 73.4%, and that in upland soil by 65.8%. Conversely, application of chemical fertiliser reduced total carbon in both soil types. There were significant positive correlations between total carbon and Zn, Cu, and Pb in rice husks grown in paddy soil (R2 = 0.95, 0.996, 0.95; p < 0.05). The content of amylose in biogas slurry treatment of paddy soil increased by 35.9%, while that in upland soil decreased by 19.2%. After biogas slurry was applied, the contents of fulvic acid- and humic acid-like substances in paddy soil average increased by 40.9% and 45.6%, while the contents of protein-like components were enhanced by 46.8% in upland soil. This result was consistent with predictions of microbial community function. Microorganisms in paddy soil generally preferred carbon fixation, while those in upland soil preferred hydrocarbon degradation and chemoheterotrophy. Understanding the changes in soil carbon stock and microbial function after biogas slurry application will contribute to sustainable agricultural development and food security.

Plastic mulch debris in rhizosphere: Interactions with soil-microbe-plant systems.

Large amounts of plastic mulch debris (PMD) accumulated in the soil can endanger agroecosystems. However, little is known about the interactions between PMD and soil-microbe-plant systems. In this study, a pot experiment (four replicates) in tropical greenhouse was conducted to investigate the effects of PMD (polyethylene) at different concentrations (0, 0.4, 0.8, 4.0, 6.0 g kg-1) on soil nutrients, rhizosphere bacterial communities and rice growth. This study further explored the interactive mechanisms between PMD and environmental factors based on correlation analysis and previous studies. The results showed that PMD continuously reduced the soil capabilities to store nutrients (C, N, P, humic-like substances) and increased the proportion of P and biodegradable dissolved organic matter (DOM). At the full ripening stage of rice growth, total organic carbon (TOC), total nitrogen (TN) and total phosphorus (TP) in all PMD treatments significantly decreased by 60.86, 52.51 and 34.83% respectively as compared to CK (p < 0.05). Furthermore, PMD increased the total abundance of bacteria but reduced the diversity and evenness of bacterial communities, which further affected microbial metabolic functions. Total OTUs and Shannon decreased 0.02-17.05% and 0.69-7.55% in treatments. At harvest-time, PMD reduced the biomass and yield of rice with 11.34 and 19.24% (all treatments on average) lower than CK. Under the influence of PMD, the order of correlation size between PMD and one environmental factor was PMD-soil > PMD-microbe > PMD-plant, and the order of correlation between two environmental factors was soil-microbe > microbe-plant > soil-plant. Over all, PMD had the most significant negative effects on soil nutrients storage, followed by the change of microbial community structure and microbial metabolic functions. The negative effects of PMD on crops were relatively weak.

Effects of plastic mulch film residues on soil-microbe-plant systems under different soil pH conditions.

Plastic mulch film residues (PMFR) accumulated throughout mulching years can result in serious environmental problems, especially in hotter areas with frequent farming (e.g. the tropics). The effects of long-term mulching on the soil-microbe-plant system, however, are largely unknown. As mulching years is positively correlated with PMFR concentrations, we used a controlled pot experiment to investigate the effects of mulching years (20a: The concentration of PMFR is about 2 g kg-1, 60a: About 6 g kg-1) on rice growth, rhizosphere bacterial communities, and soil organic carbon (SOC) under different soil pH conditions. Mulching years reduced rice growth; 20a showed more negative effects than 60a on rice tillers number and biomass. PMFR changed the composition, diversity, and metabolic function of the rhizosphere bacterial communities. The content of SOC decreased as mulching residues increased; total organic carbon (TOC), soil organic matter (SOM), Fn (355), and humification index (HIX) declined by 30.24%, 55.97%, 59.74%, and 70.24%, respectively. Furthermore, significant correlations between bacterial communities and SOC were observed in the soil-microbe-plant system. PMFR showed stronger negative effects on rice growth in acidic soil (pH 4.5); however, in basic soil (pH 8.5), there were stronger variations within the bacterial communities and a more significant decline in SOC than acidic soil (pH 4.5). The results of this study are expected to provide theoretical references for understanding of the effects of PMFR on agroecosystems and preventing and controlling plastic pollution.

Reducing bioavailability of heavy metals in contaminated soil and uptake by maize using organic-inorganic mixed fertilizer.

Heavy metals in soil are harmful to human health via the food chain, but little is known about the mechanism of reducing bioavailability of Cd or Pb to maize (Zea mays L.) by applying complex amendments to soil. A field experiment was conducted at a tropical site in Hainan Province, China, that had been subjected to soil pollution by Cd and Pb from past mining activities. There were ten treatment groups comprising a mixture of biochar, hydroxyapatite (HAP), manure, and plant ash in varying proportions and at three different rates. Compared with untreated soil, all treatments increased pH by 2-3 units in bulk soil or 1-2 units in rhizosphere soil. For all amendments, the concentration of Cd in all parts of maize plants was decreased compared with unamended soil, but this effect was much smaller for Pb. The greatest effect was found with a mixture containing the ratio of HAP:manure:biochar:plant ash as 6:4:2:1 when applied at 20.1 t ha-1. The dominant microbial group in contaminated soil was Proteobacteria. There is evidence that this group can immobilize Cd by mechanisms that include biosorption and bioprecipitation. It was concluded that the mixed amendments containing biochar, HAP, manure, and plant ash can be useful in decreasing Cd uptake by maize. The amendment in this study likely operates through a combination of soil chemical changes and by influencing the soil-microbe-plant interaction.