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Cancer poses a major threat to human health worldwide. The development of anti-tumor materials provides new modalities for cancer diagnosis and treatment. In this review, we comprehensively summarize the research progress and clinical applications of anti-tumor materials. First, we introduce the etiology and pathogenesis of cancer, and the significance and challenges of anti-tumor materials research. Then, we classify anti-tumor materials and discuss their mechanisms of action. After that, we elaborate the research advances and clinical applications of anti-tumor materials, including those targeting tumor cells and therapeutic instruments. Finally, we discuss the future perspectives and challenges in the field of anti-tumor materials. This review aims to provide an overview of the current status of anti-tumor materials research and application, and to offer insights into future directions in this rapidly evolving field, which holds promise for more precise, efficient and customized treatment of cancer.
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Antineoplásicos , Neoplasias , Humanos , Neoplasias/tratamento farmacológico , Neoplasias/terapia , Antineoplásicos/uso terapêutico , Antineoplásicos/farmacologia , Terapia de Alvo Molecular/métodos , Animais , Sistemas de Liberação de Medicamentos/métodosRESUMO
Waterborne polyurethane, renowned for its lightweight properties, excellent insulation capabilities, and corrosion resistance, has found extensive application in fields such as construction, automotive, leather, and thermal insulation. Nevertheless, during operational usage, waterborne polyurethane materials, akin to other polymeric substances, are susceptible to oxidative aging manifestations like yellowing, cracking, and diminished mechanical performance, significantly curtailing their utility. Consequently, the synthesis of yellowing-resistant polyurethane assumes pivotal significance. This study integrates dynamic reversible reactions into the synthesis process of polyurethane by introducing the dynamic reversible compound 2-hydroxyethyl disulfide as a chain extender, alongside the incorporation of a UV absorber to enhance the polyurethane's resistance to yellowing. When the disulfide bonds absorb heat, they undergo cleavage, yielding thiols that spontaneously recombine into disulfide bonds at ambient temperatures, allowing for the continuous breaking and reformation of disulfide bonds to absorb heat. Concurrently, in collaboration with the UV absorber, the detrimental effects of ultraviolet radiation on the polyurethane material are mitigated, thereby augmenting its resistance to yellowing. This study scrutinizes the positioning of UV absorber addition, the quantity of UV absorber, and the molar ratio of 1,4-butanediol to 2-hydroxyethyl disulfide, characterizing the functional groups of polyurethane through infrared and Raman spectroscopy. It is observed that the successful preparation of yellowing-resistant polyurethane is achieved, and evaluations on the modified polyurethane through color difference, tensile, and centrifugal tests reveal that the optimal yellowing resistance is attained by adding a UV absorber at a mass fraction of 1% to 3% prior to chain extension, resulting in a color change grade of 2, denoting slight discoloration. Simultaneously, the other properties of polyurethane exhibit relative stability. Notably, when the molar ratio of 1,4-butanediol to 2-hydroxyethyl disulfide is 3:2, the overall performance of the polyurethane remains stable, with exceptional yellowing resistance capabilities attaining a color change grade of 2.
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Understanding intracellular phase separation is crucial for deciphering transcriptional control, cell fate transitions, and disease mechanisms. However, the key residues, which impact phase separation the most for protein phase separation function have remained elusive. We develop PSPHunter, which can precisely predict these key residues based on machine learning scheme. In vivo and in vitro validations demonstrate that truncating just 6 key residues in GATA3 disrupts phase separation, enhancing tumor cell migration and inhibiting growth. Glycine and its motifs are enriched in spacer and key residues, as revealed by our comprehensive analysis. PSPHunter identifies nearly 80% of disease-associated phase-separating proteins, with frequent mutated pathological residues like glycine and proline often residing in these key residues. PSPHunter thus emerges as a crucial tool to uncover key residues, facilitating insights into phase separation mechanisms governing transcriptional control, cell fate transitions, and disease development.
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Aprendizado de Máquina , Proteínas , GlicinaRESUMO
The promise of electrochemically reducing excess anthropogenic carbon dioxide into useful chemicals and fuels has gained significant interest. Recently, indium-copper (In-Cu) alloys have been recognized as prospective catalysts for the carbon dioxide reduction reaction (CO2RR), although they chiefly yield carbon monoxide. Generating further reduced C1 species such as methane remains elusive due to a limited understanding of how In-Cu alloying impacts electrocatalysis. In this work, we investigated the effect of alloying In with Cu for CO2RR to form methane through first-principles simulations. Compared with pure copper, In-Cu alloys suppress the hydrogen evolution reaction while demonstrating superior initial CO2RR selectivity. Among the alloys studied, In7Cu10 exhibited the most promising catalytic potential, with a limiting potential of -0.54 V versus the reversible hydrogen electrode. Analyses of adsorbed geometries and electronic structures suggest that this decreased overpotential arises primarily from electronic perturbations around copper and indium ions and carbon-oxygen bond stability. This study outlines a rational strategy to modulate metal alloy compositions and design synergistic CO2RR catalysts possessing appreciable activity and selectivity.
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The three-dimensional structure of chromatin plays a crucial role in development and disease, both of which are associated with transcriptional changes. However, given the heterogeneity in single-cell chromatin architecture and transcription, the regulatory relationship between the three-dimensional chromatin structure and gene expression is difficult to explain based on bulk cell populations. Here we develop a single-cell, multimodal, omics method allowing the simultaneous detection of chromatin architecture and messenger RNA expression by sequencing (single-cell transcriptome sequencing (scCARE-seq)). Applying scCARE-seq to examine chromatin architecture and transcription from 2i to serum single mouse embryonic stem cells, we observe improved separation of cell clusters compared with single-cell chromatin conformation capture. In addition, after defining the cell-cycle phase of each cell through chromatin architecture extracted by scCARE-seq, we find that periodic changes in chromatin architecture occur in parallel with transcription during the cell cycle. These findings highlight the potential of scCARE-seq to facilitate comprehensive analyses that may boost our understanding of chromatin architecture and transcription in the same single cell.
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Cromatina , Cromossomos , Animais , Camundongos , RNA Mensageiro/genética , Análise de Célula Única/métodosRESUMO
The process of suspension polymerization was utilized to create acrylate resin microspheres with mesh numbers of 140-200 µm and particle sizes of 100 µm for implementation in mesh coating technology. The copolymer of methyl methacrylate (MMA) and methyl acrylate (MA) served as the primary polymer, with dibenzoyl peroxide (DBPO) functioning as the initiator, and a mixture of calcium carbonate and deionized water served as the dispersion medium. The surface morphology of the synthesized microspheres was analyzed through Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) to confirm successful synthesis. The optimal reaction conditions for the synthesis of these microspheres were determined to be a dispersant dosage of 30 g of calcium carbonate with a monomer ratio of 4:1, a reaction time of 1 h, an initiator dosage of 1.2 g of BPO, and a reaction temperature of approximately 75-80 C, resulting in microspheres with a regular spherical shape and smooth surface.
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Correction for 'High-resolution DNA size enrichment using a magnetic nano-platform and application in non-invasive prenatal testing' by Bo Zhang et al., Analyst, 2020, 145, 5733-5739, DOI: 10.1039/D0AN00813C.
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B cells played an important role in Schistosoma infection-induced diseases. TLR7 is an intracellular member of the innate immune receptor. The role of TLR7 on B cells mediated immune response is still unclear. Here, C57BL/6 mice were percutaneously infected by S. japonicum for 5-6 weeks. The percentages and numbers of B cells increased in the infected mice (p < 0.05), and many activation and function associated molecules were also changed on B cells. More splenic cells of the infected mice expressed TLR7, and B cells were served as the main cell population. Moreover, a lower level of soluble egg antigen (SEA) specific antibody and less activation associated molecules were found on the surface of splenic B cells from S. japonicum infected TLR7 gene knockout (TLR7 KO) mice compared to infected wild type (WT) mice (p < 0.05). Additionally, SEA showed a little higher ability in inducing the activation of B cells from naive WT mice than TLR7 KO mice (p < 0.05). Finally, the effects of TLR7 on B cells are dependent on the activation of NF-κB p65. Altogether, TLR7 was found modulating the splenic B cell responses in S. japonicum infected C57BL/6 mice.
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Linfócitos B/imunologia , Schistosoma japonicum/fisiologia , Esquistossomose Japônica/imunologia , Baço/imunologia , Receptor 7 Toll-Like/imunologia , Animais , Feminino , Humanos , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Schistosoma japonicum/genética , Esquistossomose Japônica/genética , Esquistossomose Japônica/parasitologia , Baço/parasitologia , Receptor 7 Toll-Like/genéticaRESUMO
BACKGROUND: Biomolecular condensates have been implicated in multiple cellular processes. However, the global role played by condensates in 3D chromatin organization remains unclear. At present, 1,6-hexanediol (1,6-HD) is the only available tool to globally disrupt condensates, yet the conditions of 1,6-HD vary considerably between studies and may even trigger apoptosis. RESULTS: In this study, we first analyzed the effects of different concentrations and treatment durations of 1,6-HD and found that short-term exposure to 1.5% 1,6-HD dissolved biomolecular condensates whereas long-term exposure caused aberrant aggregation without affecting cell viability. Based on this condition, we drew a time-resolved map of 3D chromatin organization and found that short-term treatment with 1.5% 1,6-HD resulted in reduced long-range interactions, strengthened compartmentalization, homogenized A-A interactions, B-to-A compartment switch and TAD reorganization, whereas longer exposure had the opposite effects. Furthermore, the long-range interactions between condensate-component-enriched regions were markedly weakened following 1,6-HD treatment. CONCLUSIONS: In conclusion, our study finds a proper 1,6-HD condition and provides a resource for exploring the role of biomolecular condensates in 3D chromatin organization.
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Condensados Biomoleculares/efeitos dos fármacos , Cromatina , Glicóis/farmacologia , Condensados Biomoleculares/química , Fenômenos Fisiológicos Celulares , Glicóis/química , Células HeLa , Humanos , Imageamento TridimensionalRESUMO
Potassium-ion batteries (KIBs) are promising electrochemical energy storage systems because of their low cost and high energy density. However, practical exploitation of KIBs is hampered by the lack of high-performance cathode materials. Here we report a potassium manganese hexacyanoferrate (K2Mn[Fe(CN)6]) material, with a negligible content of defects and water, for efficient high-voltage K-ion storage. When tested in combination with a K metal anode, the K2Mn[Fe(CN)6]-based electrode enables a cell specific energy of 609.7 Wh kg-1 and 80% capacity retention after 7800 cycles. Moreover, a K-ion full-cell consisting of graphite and K2Mn[Fe(CN)6] as anode and cathode active materials, respectively, demonstrates a specific energy of 331.5 Wh kg-1, remarkable rate capability, and negligible capacity decay for 300 cycles. The remarkable electrochemical energy storage performances of the K2Mn[Fe(CN)6] material are attributed to its stable frameworks that benefit from the defect-free structure.
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The accumulation of myeloid-derived suppressor cells (MDSCs) is one of the major obstacles to achieve an appropriate anti-tumor immune response and successful tumor immunotherapy. MDSCs in tumor-bearing hosts are primarily polymorphonuclear (PMN-MDSCs). However, the mechanisms regulating the development of MDSCs remain poorly understood. In this report, we showed that interferon regulatory factor 4 (IRF4) plays a key role in the development of PMN-MDSCs, but not monocytic MDSCs. IRF4 deficiency caused a significant elevation of PMN-MDSCs and enhanced the suppressive activity of PMN-MDSCs, increasing tumor growth and metastasis in mice. Mechanistic studies showed that c-Myc was up-regulated by the IRF4 protein. Over-expression of c-Myc almost abrogated the effects of IRF4 deletion on PMN-MDSCs development. Importantly, the IRF4 expression level was negatively correlated with the PMN-MDSCs frequency and tumor development but positively correlated with c-Myc expression in clinical cancer patients. In summary, this study demonstrated that IRF4 represents a novel regulator of PMN-MDSCs development in cancer, which may have predictive value for tumor progression.
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Fatores Reguladores de Interferon/fisiologia , Células Supressoras Mieloides/fisiologia , Neoplasias/imunologia , Proteínas Proto-Oncogênicas c-myc/genética , Transcrição Gênica , Animais , Proliferação de Células , Feminino , Camundongos , Camundongos Endogâmicos C57BL , Metástase Neoplásica , Proteínas Proto-Oncogênicas c-myc/fisiologiaRESUMO
Electrochemical carbon dioxide reduction reaction (CO2 RR) represents a promising way to generate fuels and chemical feedstock sustainably. Recently, studies have shown that two-dimensional metal carbides and nitrides (MXenes) can be promising CO2 RR electrocatalysts due to the alternating -C and -H coordination with intermediates that decouples scaling relations seen on transition metal catalysts. However, further by tuning the electronic and surface structure of MXenes it should still be possible to reach higher turnover number and selectivities. To this end, defect engineering of MXenes for electrochemical CO2 RR has not been investigated to date. In this work, first-principles modelling simulations are employed to systematically investigate CO2 RR on M2 XO2 -type MXenes with transition metal and carbon/nitrogen vacancies. We found that the -C-coordinated intermediates take the form of fragments (e. g., *COOH, *CHO) whereas the -H-coordinated intermediates form a complete molecule (e. g., *HCOOH, *H2 CO). Interestingly, the fragment-type intermediates become more strongly bound when transition-metal vacancies are present on most MXenes, while the molecule-type intermediates are largely unaffected, allowing the CO2 RR overpotential to be tuned. The most promising defective MXene is Hf2 NO2 containing Hf vacancies, with a low overpotential of 0.45â V. More importantly, through electronic structure analysis it could be observed that the Fermi level of the MXene changes significantly in the presence of vacancies, indicating that the Fermi level shift can be used as an ideal descriptor to rapidly predict the catalytic performance of defective MXenes. Such an evaluation strategy is applicable to other catalysts beyond MXenes, which could enhance high throughput screening efforts for accelerated catalyst discovery.
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Precise DNA sizing can boost sequencing efficiency, reduce cost, improve data quality, and even allow sequencing of low-input samples, while current pervasive DNA sizing approaches are incapable of differentiating DNA fragments under 200 bp with high resolution (<20 bp). In non-invasive prenatal testing (NIPT), the size distribution of cell-free fetal DNA in maternal plasma (main peak at 143 bp) is significantly different from that of maternal cell-free DNA (main peak at 166 bp). The current pervasive workflow of NIPT and DNA sizing is unable to take advantage of this 20 bp difference, resulting in sample rejection, test inaccuracy, and restricted clinical utility. Here we report a simple, automatable, high-resolution DNA size enrichment workflow, named MiniEnrich, on a magnetic nano-platform to exploit this 20 bp size difference and to enrich fetal DNA fragments from maternal blood. Two types of magnetic nanoparticles were developed, with one able to filter high-molecular-weight DNA with high resolution and the other able to recover the remaining DNA fragments under the size threshold of interest with >95% yield. Using this method, the average fetal fraction was increased from 13% to 20% after the enrichment, as measured by plasma DNA sequencing. This approach provides a new tool for high-resolution DNA size enrichment under 200 bp, which may improve NIPT accuracy by rescuing rejected non-reportable clinical samples, and enable NIPT earlier in pregnancy. It also has the potential to improve non-invasive screening for fetal monogenic disorders, differentiate tumor-related DNA in liquid biopsy and find more applications in autoimmune disease diagnosis.
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Ácidos Nucleicos Livres , Diagnóstico Pré-Natal , DNA/genética , Feminino , Humanos , Fenômenos Magnéticos , Gravidez , Análise de Sequência de DNARESUMO
Inorganic cesium lead halide (CsPbI3) is a promising candidate for next-generation photovoltaic devices, but photoactive α-phase CsPbI3 can rapidly transform to non-photoactive yellow δ-CsPbI3 in a humid atmosphere. Here, we report that partial substitution of cesium by the potassium or rubidium element can effectively improve the phase stability against moisture by forming a water-repelling surface layer with Rb/K segregation. Using density functional theory, we found that the water-induced polarization, which triggers the PbI62- octahedron distortion and accelerates the phase transition, can be effectively alleviated by incorporating Rb/K elements. Further exploration of transition states suggests that Rb/K doped surface layers result in a higher activation barrier for water penetration. The electronic structure analysis further reveals that the barrier enhancement originates from the absence of the participation of inner 5p electrons in Rb/K-H2O binding, which induces a much lower energy barrier in pristine CsPbI3. Based on these improvements, the doped perovskites remained in the major α-phase after direct exposure to ambient air (RH â¼ 30%) for 5 hours, while pristine CsPbI3 showed an irreversible degradation. With the clarified mechanism of enhanced phase stability of Rb/K incorporation, we suggest such a doping method as a promising strategy to be widely applied in the field of photovoltaic devices.
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CD4+ T follicular helper (Tfh) cells, a new subset of immune cells, have been demonstrated to be involved in granulomatous responses to Schistosoma japonicum (S. japonicum) infection. However, the role and underlying mechanisms of Tfh cell aggregation in S. japonicum infection remain incompletely understood. In this study, we provide evidence that S. japonicum infection enhances the accumulation of Tfh cells in the spleen, lymph nodes, and peripheral blood of C57BL/6 mice. Infection-induced Tfh cells exhibited more potent effects directly on B cell responses than the control Tfh cells (P < 0.05). Furthermore, reduced apoptosis of Tfh cells was found both in S. japonicum infected mice and in soluble egg antigen (SEA) treated Tfh cells (P < 0.05). Mechanistic studies reveal that caspase-3 is the primary drivers of down-regulated apoptotic Tfh cell death in S. japonicum infection. In summary, this study demonstrates that Tfh cell accumulation might have an impact on the generation of immune responses in S. japonicum infection, and caspase-3 signaling mediated apoptosis down-regulation might responsible for the accumulation of Tfh cell in this course.
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Apoptose/imunologia , Caspase 3/imunologia , Schistosoma japonicum/imunologia , Esquistossomose Japônica/imunologia , Linfócitos T Auxiliares-Indutores/imunologia , Animais , Caspase 3/metabolismo , Regulação para Baixo/imunologia , Feminino , Linfonodos/imunologia , Linfonodos/metabolismo , Linfonodos/parasitologia , Camundongos Endogâmicos C57BL , Schistosoma japonicum/fisiologia , Esquistossomose Japônica/metabolismo , Esquistossomose Japônica/parasitologia , Baço/imunologia , Baço/metabolismo , Baço/parasitologia , Linfócitos T Auxiliares-Indutores/metabolismo , Linfócitos T Auxiliares-Indutores/parasitologiaRESUMO
Enzymatic reactions in living cells are highly dynamic but simultaneously tightly regulated. Enzyme engineers seek to construct multienzyme complexes to prevent intermediate diffusion, to improve product yield, and to control the flux of metabolites. Here we choose a pair of short peptide tags (RIAD and RIDD) to create scaffold-free enzyme assemblies to achieve these goals. In vitro, assembling enzymes in the menaquinone biosynthetic pathway through RIAD-RIDD interaction yields protein nanoparticles with varying stoichiometries, sizes, geometries, and catalytic efficiency. In Escherichia coli, assembling the last enzyme of the upstream mevalonate pathway with the first enzyme of the downstream carotenoid pathway leads to the formation of a pathway node, which increases carotenoid production by 5.7 folds. The same strategy results in a 58% increase in lycopene production in engineered Saccharomyces cerevisiae. This work presents a simple strategy to impose metabolic control in biosynthetic microbe factories.
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Reatores Biológicos/microbiologia , Escherichia coli/metabolismo , Engenharia Metabólica/métodos , Engenharia de Proteínas/métodos , Saccharomyces cerevisiae/metabolismo , Biocatálise , Vias Biossintéticas/genética , Carotenoides/metabolismo , Escherichia coli/enzimologia , Escherichia coli/genética , Licopeno/metabolismo , Ácido Mevalônico/metabolismo , Saccharomyces cerevisiae/enzimologia , Saccharomyces cerevisiae/genética , Vitamina K 2/metabolismoRESUMO
Multienzyme complexes, or metabolons, are assemblies or clusters of sequential enzymes that naturally exist in metabolic pathways. These nanomachineries catalyze the conversion of metabolites more effectively than the freely floating enzymes by minimizing the diffusion of intermediates in vivo. Bioengineers have devised synthetic versions of multienzyme complexes in cells to synergize heterologous biosynthesis, to improve intracellular metabolic flux, and to achieve higher titer of valuable chemical products. Here, we utilized orthogonal protein reactions (SpyCatcher/SpyTag and SnoopCatcher/SnoopTag pairs) to covalently assemble three key enzymes in the mevalonate biosynthesis pathway and showed 5-fold increase of lycopene and 2-fold increase of astaxanthin production in Escherichia coli. The multienzyme complexes are ellipsoidal nanostructures with hollow interior space and uniform thickness and shapes. Intracellular covalent enzyme assembly has yielded catalytic nanomachineries that drastically enlarged the flux of carotenoid biosynthesis in vivo. These studies also deepened our understanding on the complexity of hierarchical enzyme assembly in vivo.
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Biocatálise , Vias Biossintéticas , Complexos Multienzimáticos/metabolismo , Nanoestruturas/química , Sequência de Aminoácidos , Carotenoides/química , Carotenoides/metabolismo , Complexos Multienzimáticos/química , Complexos Multienzimáticos/ultraestrutura , Proteínas/metabolismoRESUMO
Immune responses play an important role in the pathogenesis of polycystic ovary syndrome (PCOS). However, the characteristics of T lymphocyte subsets in PCOS remain insufficiently understood. In this study, lymphocytes of follicular fluid (FF) were obtained from oocyte retrieval before in-vitro fertilization (IVF) in infertile women with or without PCOS. The levels of cluster of differentiation 25 (CD25), CD69, programmed death 1 (PD-1), interferon-γ (IFN-γ), interleukin 17A (IL-17A) and IL-10 in T lymphocytes were determined by flow cytometry. Our results showed that the percentage of FF CD8+ T cells was significantly decreased in infertile patients with PCOS (P < 0.05). Furthermore, the levels of CD69 and IFN-γ were significantly decreased and the level of PD-1 was increased in both CD4+ and CD8+ T cells from infertile patients with PCOS (P < 0.05). Moreover, the expression of PD-1 on CD4+ or CD8+ T cells was positively correlated with the estradiol (E2) levels in the serum and reversely correlated with the expression of IFN-γ in CD4+ or CD8+ T cells in infertile patients with PCOS. These results suggested that T cell dysfunction may be involved in the pathogenesis of PCOS.
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Líquido Folicular/imunologia , Síndrome do Ovário Policístico/imunologia , Subpopulações de Linfócitos T/imunologia , Adulto , Antígenos CD/análise , Antígenos CD/imunologia , Antígenos de Diferenciação de Linfócitos T/análise , Antígenos de Diferenciação de Linfócitos T/imunologia , Linfócitos T CD4-Positivos/imunologia , Linfócitos T CD8-Positivos/imunologia , Citocinas/análise , Citocinas/imunologia , Feminino , Fertilização in vitro , Humanos , Infertilidade Feminina/complicações , Infertilidade Feminina/imunologia , Subunidade alfa de Receptor de Interleucina-2/análise , Subunidade alfa de Receptor de Interleucina-2/imunologia , Lectinas Tipo C/análise , Lectinas Tipo C/imunologia , Síndrome do Ovário Policístico/complicações , Adulto JovemRESUMO
Graphene/metal oxides (G/MO) composite materials have attracted much attention as the anode of sodium ion batteries (SIBs), because of the high theoretical capacity. However, most metal oxides operate based on the conversion mechanism and the alloying mechanism has changed to Na2O after the first cycle. The influence of G/Na2O (G/N) on the subsequent sodiation process has never been clearly elucidated. In this work, we report a systematic investigation on the G/N interface from both aspects of theoretical simulation and experiment characterization. By applied first-principles simulations, we find that the sluggish kinetics in the G/MO materials is mainly caused by the high diffusion barrier (0.51 eV) inside the Na2O bulk, while the G/N interface shows a much faster transport kinetics (0.25 eV) via unique double-interstitialcy mechanism. G/N interface possesses an interfacial storage of Na atom through the charge separation mechanism. The experimental evidence confirms that high interfacial ratio structure of G/N greatly improves the rate performance and endows G/MO materials the interfacial storage. Furthermore, the experimental investigation finds that the high interfacial ratio structure of G/N also benefits from the reversible reaction between SnO2 and Sn during cycling. Lastly, the effects of (N, O, S) doping in graphene systems at the G/N interface were also explored. This work provides a fundamental comprehension on the G/MO interface structure during the sodiation process, which is helpful to design energy storage materials with high rate performance and large capacity.
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Toll-like receptors (TLRs) play an important role in regulating immune responses during pathogen infection. However, roles of TLRs on T cells reside in the mesenteric lymph node (MLN) were not be fully elucidated in the course of S. japonicum infection. In this study, T lymphocytes from the mesenteric lymph node (MLN) of S. japonicum-infected mice were isolated and the expression and roles of TLR2, TLR3, TLR4, and TLR7 on both CD4+ and CD8+ T cells were compared. We found that the expression of TLR7 was increased in the MLN cells of S. japonicum-infected mice, particularly in CD4+ and CD8+ T cells (P < 0.05). R848, a TLR7 agonist, could enhance the production of IFN-γ from MLN T cells of infected mice (P < 0.05), especially in CD8+ T cells (P < 0.01). In TLR7 gene knockedout (KO) mice, the S. japonicum infection caused a significant decrease (P < 0.05) of the expression of CD25 and CD69, as well as the production of IFN-γ and IL-4 inducted by PMA plus ionomycin on both CD4+ and CD8+ T cells. Furthermore, the decreased level of IFN-γ and IL-4 in the supernatants of SEA- or SWA-stimulated mesenteric lymphocytes was detected (P < 0.05). Our results indicated that S. japonicum infection could induce the TLR7 expression on T cells in the MLN of C57BL/6 mice, and TLR7 mediates T cell response in the early phase of infection.