Aspirin enhances IFN-α-induced growth inhibition and apoptosis of hepatocellular carcinoma via JAK1/STAT1 pathway.

STAT1 has a key role in exerting the antiproliferative and proapoptotic effects of interferon (IFN)-α on tumors, and its defects in expression is associated with IFN-α resistance. In this study we want to investigate whether aspirin can improve the antitumor efficiency of IFN-α on hepatocellular carcinoma (HCC) through the activation of STAT1. We found that aspirin not only significantly enhanced IFN-α-induced antiproliferation and apoptosis of HCC in vitro study but also enhanced tumor growth inhibition in nude mice. Although IFN-α alone resulted in significant phosphorylation of both STAT1 and STAT3, aspirin only prompted the IFN-α-induced phosphorylation of STAT1. Further study revealed that aspirin-prompted phosphorylation of STAT1 was activated through phosphorylation of JAK1. Furthermore, aspirin-activated STAT1 upregulated the transcription of proapoptotic IFN-stimulated gene (ISG) of X-linked inhibitor of apoptosis-associated factor-1 and downregulated the transcription of antiapoptotic ISG of G1P3, which in turn promoted the expression of Bax and activation of caspase-9 and caspase-3, thereby sensitizing HCC cells to IFN-α-induced apoptosis. Taken together, our findings suggest a novel strategy of using aspirin to overcome tumor resistance and enhance the effectiveness of IFN-α in HCC treatment through activating STAT1 gene, and have potential implications for improving future IFN-α protein and gene therapy.

Surface integrity and removal mechanism in simulated dental finishing of a feldspathic porcelain.

Dental abrasive finishing of a fine-grained feldspathic porcelain was performed on a computer-assisted apparatus for simulation of a 2-degrees-of-freedom restorative operation with a dental handpiece and a coarse diamond bur of grit size of 106-125 mum. Finishing forces, surface roughness, and morphology were investigated as functions of finishing conditions. The tangential and normal forces were measured using a piezoelectric dynamometer and a data processing system. The results indicated that these forces increased with either the depth of cut or with the feed rate, in the ranges of 0.12-0.31 N and 0.45-1.09 N, respectively. However, an increase in either depth of cut or feed rate affected neither the surface roughness measured using a stylus profilometer nor the morphology observed under a scanning electron microscope. The finished porcelain surfaces were found to consist of the microfracture and chipping areas, ductile removal areas, smeared areas, and debris. Irregular fracture and chipping resulted from the extension of lateral/median cracks; ductile micromachining was attributed to the plastic deformation accompanied by distributed microcracks. It was determined that a combination of the microfracture and ductile micromachining was the primary mechanism for material removal.

Performance evaluation of a dental handpiece in simulation of clinical finishing using a novel 2DOF in vitro apparatus.

This paper reports on the performance evaluation of a dental handpiece in simulation of clinical finishing using a novel two-degrees-of-freedom (2DOF) in vitro apparatus. The instrumented apparatus consisted of a two-dimensional computer-controlled coordinate worktable carrying a dental handpiece, a piezoelectric force dynamometer, and a high-speed data acquisition and signal conditioning system for simulating the clinical operations and monitoring the dental finishing processes. The performance of the dental handpiece was experimentally evaluated with respect to rotational speed, torque, and specific finishing energy under the applied clinical finishing conditions. The results show that the rotational speeds of the dental handpiece decreased by increasing either the depth of cut or the feed rate at a constant clinically applied air pressure and water flowrate. They also decreased when increasing both the tangential and normal finishing forces. The specific finishing energy decreased with an increase in either depth of cut or feed rate, while the finishing torque increased as either the depth of cut or the feed rate was increased. Implications of these results were to provide guidance for proper applications of dental handpieces in clinical practice.

[The relation of typing of gastric carcinoma according to traditional Chinese medicine theories and clinical pathologic classification].

102 cases of gastric carcinoma which have been removed by partial gastrectomy and have pathological diagnoses and follow-up results are reported. The relationship between their preoperational syndromes of TCM and pathological, TNM classification and post-operational survival rate has been studied. There is indication that the type of Ganwei Buhe (the dispersing function of the liver is disturbed and affects the stomach) manifests the earlier stage of the gastric carcinoma and the majority of cases are in stage 1 or 2 in TNM, the 5-year survival rate with surgery being 46%. The majority of cases of the types of Pishen Yangxu (deficiency of energy in the Spleen and Kidney), Yinxu Neire (the febrile syndrome of the viscera caused by the insufficient primordial energy) and Qixue Shuangkui (deficiency of both energy and blood) are in stage 3 or 4 in TNM, the 5-year survival rate with surgery differently being 5,8,12% respectively. The type of Qizhi Xueyu (energy stagnancy and blood stasis) is between the abovementioned two groups, with a 5-year survival rate of 40%.