Safety and efficacy of stoma site selection in CT-guided percutaneous gastrostomy: a retrospective analysis.

PURPOSE: To compare the safety and efficacy of CPG in the rectus abdominis and intercostal regions. MATERIALS AND METHODS: This retrospective study included 226 patients who underwent CPG at a single center, with the stoma placed in the rectus abdominis or intercostal region. Surgical outcomes and complications, such as pain and infection within 6 months postoperatively, were recorded. RESULTS: The surgical success rate was 100%, and the all-cause mortality rate within 1 month was 0%. An intercostal stoma was placed in 56 patients; a rectus abdominis stoma was placed in 170 patients. The duration of surgery was longer for intercostal stoma placement (37.66 ± 14.63 min) than for rectus abdominis stoma placement (30.26 ± 12.40 min) (P = 0.000). At 1 month postsurgery, the rate of stoma infection was greater in the intercostal group (32.1%) than in the rectus abdominis group (20.6%), but the difference was not significant (P = 0.077). No significant difference was observed in the infection rate between the two groups at 3 or 6 months postsurgery (P > 0.05). Intercostal stoma patients reported higher pain scores during the perioperative period and at 1 month postsurgery (P = 0.000), but pain scores were similar between the two groups at 3 and 6 months postsurgery. The perioperative complication rates for intercostal and rectus abdominis surgery were 1.8% and 5.3%, respectively (P = 0.464), with no significant difference in the incidence of tube dislodgement (P = 0.514). Patient weight improved significantly at 3 and 6 months postoperatively compared to preoperatively (P < 0.05). CONCLUSION: Rectus abdominis and intercostal stomas have similar safety and efficacy. However, intercostal stomas may result in greater short-term patient discomfort.

Long non-coding RNA PRR7-AS1 promotes osteosarcoma progression via binding RNF2 to transcriptionally suppress MTUS1.

Introduction: Osteosarcoma is a common bone malignant tumor in adolescents with high mortality and poor prognosis. At present, the progress of osteosarcoma and effective treatment strategies are not clear. This study provides a new potential target for the progression and treatment of osteosarcoma. Methods: The relationship between lncRNA PRR7-AS1 and osteosarcoma was analyzed using the osteosarcoma databases and clinical sample testing. Cell function assays and tumor lung metastasis were employed to study the effects of PRR7-AS1 on tumorigenesis in vivo and in vitro. Potential downstream RNF2 of PRR7-AS1 was identified and explored using RNA pulldown and RIP. The GTRD and KnockTF database were used to predict the downstream target gene, MTUS1, and ChIP-qPCR experiments were used to verify the working mechanismy. Rescue experiments were utilized to confirm the role of MTUS1 in the pathway. Results: Deep mining of osteosarcoma databases combined with clinical sample testing revealed a positive correlation between lncRNA PRR7-AS1 and osteosarcoma progression. Knockdown of PRR7-AS1 inhibited osteosarcoma cell proliferation and metastasis in vitro and in vivo. Mechanistically, RNA pulldown and RIP revealed that PRR7-AS1 may bind RNF2 to play a cancer-promoting role. ChIP-qPCR experiments were utilized to validate the working mechanism of the downstream target gene MTUS1. RNF2 inhibited the transcription of MTUS1 through histone H2A lysine 119 monoubiquitin. Rescue experiments confirmed MTUS1 as a downstream direct target of PRR7-AS1 and RNF2. Discussion: We identified lncRNA PRR7-AS1 as an important oncogene in osteosarcoma progression, indicating that it may be a potential target for diagnosis and prognosis of osteosarcoma.

Iodine-125 brachytherapy for a desmoid tumor in the abdomen-thorax: A retrospective study.

PURPOSE: To investigate the safety and efficacy of iodine-125 seed implantation in the treatment of abdomen-thorax desmoid tumors (DTs). METHODS AND MATERIALS: Data from 14 DT patients who received brachytherapy with iodine-125 seeds were retrospectively collected from 2014 to 2020. The operation was completed using CT guidance and the treatment plan system (TPS). The number of lesions and the target dosimetry parameters were recorded. After brachytherapy, the lesions were evaluated using response evaluation criteria in solid tumors (RECIST). RESULTS: Fourteen patients with 18 lesions were enrolled in this study; eleven lesions were in the thorax, seven were in the abdomen, and the lesion gross tumor volume (GTV) was 82.10 cc (interquartile range [IQR]: 40.37, 203.42 cc). The median number of seeds was 88 (IQR: 35, 158), and the median prescription dose was 120 Gy (IQR: 115, 120 Gy). The D90 was 123 ± 16.7 Gy, the V90 was 97% (IQR: 95.00, 97.25%), and the V200 was 27% (IQR: 14.50, 33.00%). The median follow-up time for each lesion was 34 (IQR: 23, 67) months, and the local response rate was 100%. Following brachytherapy, the overall survival was 52.3 ± 30.72 months. One year after brachytherapy, one patient experienced persistent worsening of a brachial plexus injury; another received a ureteral stent. No brachytherapy-related complications were observed in the remaining patients. CONCLUSIONS: Iodine-125 brachytherapy is a novel treatment option for DT of the abdomen and thorax. Although it is a safe and effective treatment, the radiation dose of iodine-125 brachytherapy for DT-embedded organs at risk must be investigated further.

Aggressive angiomyxoma of the pelvis and abdominal wall: Dramatic response to chemical ablation therapy.

Objective: Aggressive angiomyxoma (AAM) is a rare, locally aggressive soft tissue neoplasm with a marked tendency for local recurrence after surgery. Although hormone therapy, radiation therapy, and vascular embolization can be performed, we investigated the safety and efficacy of a new chemical ablation protocol for AAM. Methods: This study included two female AAM patients from 2012 to 2016. The patients' clinical and imaging data were collected. The amount of anhydrous ethanol and glacial acetic acid used for chemical ablation was documented, and the management of any complications was detailed. Results: The maximum dimensions of the residual tumor were 12.6 cm and 14.0 cm. In one case, the lesion was in the pelvis and protruded into the vulva. Eighty milliliters of liquid with a mixture of glacial acetic acid, anhydrous ethanol, and iohexol (10:9:1) was used for chemical ablation therapy via multipoint injections with a single needle. However, a pelvic fistula developed 1 month later. In another case, the lesion was located in the abdominal wall. The ablation procedure was improved by performing chemical ablation therapy with multiple needles for multi-point injections of smaller than 30 ml injections for each procedure. To date, no recurrence or metastasis has been observed in the two cases. Conclusion: The preferred treatment for AAM is complete resection. Chemical ablation therapy is a novel adjuvant therapy for AMM. Nonetheless, more research is needed to confirm these findings.

The complete mitochondrial genome of speckled dace, Rhinichthys osculus.

In this study, we report the complete mitochondrial genome sequence of speckled dace, Rhinichthys osculus, for the first time. The length of this genome is 16 611 bp, and the mitochondrial genome harbors 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNA), two ribosomal RNA genes (rRNA), and one control region (D-loop), and it has a base composition of A (30.7%), G (16.3%), C (26.5%), and T (26.5%), indicating that the percentage of A + T (57.2%) was higher than that of G + C (42.8%). Most of the genes were located on the H-strand except for the ND6 gene and eight tRNA genes. The phylogenetic analysis showed that the R. osculus mitochondrial genome formed a cluster with the other seven Rhodeus genus genomes. The mitochondrial genome of R. osculus presented here will contribute to a better understanding of the population genetics.

The polymorphism of ARNTL2 (BMAL2) gene rs2306074 C>T is associated with susceptibility of Alzheimer disease in Chinese population.

In the present study, we investigated whether polymorphism of ARNTL2 (BMAL2) gene rs2306074 T/C was associated with susceptibility of Alzheimer disease (AD) in Chinese population. A case-control method was employed in this study. 296 unrelated AD patients and 423 control subjects were recruited in current study. The prevalence of C carriers in BMAL2 gene rs2306074 T/C in AD patients was significantly higher than that of control subjects in both the whole sample and APOE ε 4 non-carriers (in the whole sample: χ (2) = 5.938, P = 0.012; in APOE ε 4 non-carriers: χ (2) = 9.048, P < 0.0001). In addition, both in the whole sample and APOE ε 4 non-carriers, prevalence of CC genotypes in BMAL2 gene rs2306074 of AD patients was also significantly higher than that in controls (in the whole sample: χ (2) = 5.126, P = 0.018; in APOE ε 4 non-carriers: χ (2) = 7.389, P = 0.023). However, there was no significant difference of prevalence of C carriers and CC genotypes in BMAL2 gene rs2306074 T/C between AD patients and control subjects among APOE ε 4 carriers (C carriers: χ (2) = 0.020, P = 0.900; CC genotypes: χ (2) = 0.017, P = 0.946). C carriers in BMAL2 gene rs2306074 T/C are associated with a high susceptibility of AD among APOE ε 4 non-carriers but not among APOE ε 4 carriers in Chinese population.

Association of fasting serum insulin and fasting serum glucose levels with cognitive impairment in Chinese nonagenarians/centenarians.

In this study, we examined the association of fasting serum insulin (FSI) and fasting serum glucose (FSG) with cognitive impairment in the very elderly using a sample of Chinese nonagenarians/centenarians. This study used data from a survey that was conducted in 2005 on all residents aged 90 years or more in a district with 2,311,709 inhabitants. FSG, FSI, and cognitive function were analyzed. The sample included 661 unrelated Chinese individuals (aged 90-108 years; mean, 93.52 ± 3.37 years; 67.17 % women; FSI, 6.27 ± 2.27 mU/mL; FSG levels, 4.46 ± 1.45 mmol/L). The prevalence of cognitive impairment was 61.81 % and that of hypoinsulinemia was 31.92 %. Individuals with hypoinsulinemia showed lower cognitive function scores (14.81 ± 5.79 vs. 15.78 ± 5.24, t = 2.160, P = 0.031). No differences in cognitive function score between different FSI and FSG groups were significant, and no differences in FSI and FSG between individuals with and without cognitive impairment were statistically significant. Unadjusted multiple logistic regressions showed that hypoinsulinemia, impaired fasting glucose, or diabetes did not change the risk of cognitive impairment significantly. In summary, we found that in elderly subjects, cognitive function appeared associated with FSI, and higher FSI may be associated with enhanced cognitive function.

Cigarette smoking is associated with increased diastolic blood pressure among Chinese nonagenarians/centenarians.

OBJECTIVE: Cigarette smoking has been confirmed as a factor influencing arterial blood pressure. In the present study, we studied whether cigarette smoking habits were still associated with arterial blood pressure among Chinese nonagenarians/centenarians. METHODS: The present study analyzed data from a survey conducted on all residents aged 90 years or more in the DuJiangYan district (in total 2,311,709 inhabitants) in 2005. RESULTS: The individuals included in the statistical analysis were 216 men and 445 women. Individuals who were heavy smokers (76.62 ± 13.28 mmHg) had higher diastolic blood pressure, compared with medium and light smokers (72.33 ± 12.98 and 70.28 ± 10.31 mmHg) (F = 3.551, p = 0.030). There was a higher prevalence of diastolic hypertension (21.62% vs 5.75% and 7.14%, χ(2 =) 6.302, p = 0.043). Furthermore, there was a higher risk for diastolic hypertension in heavy smokers (OR = 3.886, 95% CI 1.241-12.161) (adjusted) compared with medium (OR = 1.475, 95% CI 0.599-3.360) and light smokers (1.00 reference). There was, however, no significant difference in systolic blood pressure or prevalence of systolic hypertension among the different smoking groups. CONCLUSIONS: In summary, we found that among Chinese nonagenarians/centenarians, heavy smoking (current or former) could increase diastolic blood pressure and prevalence of diastolic hypertension, but was not associated with changes in systolic blood pressure.

The polymorphism of CLOCK gene 3111T/C C>T is associated with susceptibility of Alzheimer disease in Chinese population.

OBJECTIVES: In the present study, it was examined whether polymorphism of circadian locomotor output cycle kaput (CLOCK) gene 3111T/C was associated with susceptibility of Alzheimer disease (AD). METHODS: This study was conducted using a case-control method. Genotypes of apolipoprotein E (APOE e4) and CLOCK gene 3111T/C were determined by restriction fragment length polymorphism method and TaqMan assay method, respectively. RESULTS: In this study, we gathered 296 unrelated AD patients and 423 control subjects. Both in the whole sample and APOE e4 noncarriers, prevalence of C carriers in CLOCK gene 3111T/C in AD patients was significantly higher than that in control subjects (in the whole sample: χ = 37.615, P < 0.0001; in APOE e4 noncarriers: χ = 46.809, P < 0.0001). However, among APOE e4 carriers, prevalence of C carriers in CLOCK gene 3111T/C between patients and control subjects presents nonsignificant difference (χ = 0.812, P = 0.451). CONCLUSIONS: Among APOE e4 noncarriers, C carriers in CLOCK gene 3111T/C were associated with a high susceptibility of AD, but among APOE e4 carriers, the functional polymorphism of CLOCK gene 3111T/C was not associated with the susceptibility of AD.

Hypothalamic-pituitary-thyroid axis in patients with Alzheimer disease (AD).

OBJECTIVE: We observed the function of hypothalamic-pituitary-thyroid axis in patients with Alzheimer disease (AD) using a case-control study. METHODS: The case was a cohort that included 50 patients with AD. For each case subject, 1 control who was of similar age, sex, daily activities (scale of Lawton), sleep quality (Pittsburgh Sleep Quality Index), and depression (15-item Geriatrics Depression Scale) was recruited. Thyrotropin-releasing hormone (TRH), thyroid-stimulating hormone (TSH), total triiodothyronine (TT3), total tetraiodothyronine (TT4), free triiodothyronine (FT3), and free tetraiodothyronine (FT4) were detected using radioimmunity. RESULTS: Compared with the healthy controls, the patients with AD had significantly lower levels of TRH (67.72 ± 18.44 vs 78.64 ± 14.31 pmol/L; t = 2.078; P = 0.036), TSH (3.89 ± 1.22 vs 4.31 ± 1.07 mIU/L; t = 2.331; P = 0.024), TT3 (1.44 ± 0.21 vs 1.63 ± 0.19 nmol/L; t = 3.761; P = 0.018), TT4 (119.71 ± 18.64 nmol/L vs 129.54 ± 23.17 nmol/L; t = 1.328; P = 0.044), FT3 (4.01 ± 1.27 vs 5.41 ± 0.99 pmol/L; t = 4.976; P = 0.008), and FT4 (9.84 ± 1.56 vs 12.96 ± 2.20 pmol/L; t = 5.381; P = 0.006). In the AD cases, none of the correlations between TRH and TSH, TT3, TT4, FT3, and FT4, and between TSH and TT3, TT4, FT3, FT4 was significant. However, in the healthy controls, TRH was significantly correlated with TSH (R = 0.020; P = 0.042) and FT4 (R = 0.015; P = 0.018), and TSH was significantly correlated with TT4 (R = 0.209; P = 0.017) and FT4 (R = 0.215; P = 0.009). CONCLUSION: Alzheimer disease was associated with abnormal function of the hypothalamic-pituitary-thyroid axis.

Outcome and prognosis of myelopathy and radiculopathy from osteochondroma in the mobile spine: a report on 14 patients.

STUDY DESIGN: A retrospective study of 14 spinal osteochondroma (OC) patients with compressive myelopathy or radiculopathy who underwent excision of the lesions. OBJECTIVE: To evaluate the surgical results of a series of cases of symptomatic OCs and the risk of recurrence and malignant transformation in the mobile spine. SUMMARY OF BACKGROUND DATA: Intraspinal OC with symptomatic spinal cord or nerve root compression is a rare disease. Most of the lesions require surgical treatment. However, some patients may relapse or malignant transformation may occur after surgery. METHODS: Fourteen symptomatic spinal OC cases, including 2 hereditary multiple exostoses, were treated surgically from 2001 to 2010. The clinical history, plain radiographs, computed tomography, magnetic resonance imaging, pathologic characteristics, surgical treatments, and outcomes were reviewed. All the patients were followed up for an average of 48.9 months. RESULTS: The 14 patients included 8 with lesions in the cervical spine, 3 with lesions in the thoracic spine, and 2 with lesions in the lumbar spine. One patient had a particularly large lesion which extended from C7 to T6. The OCs originated from the lamina (4), lateral mass (3), vertebral body (3), processus transversus (1), spinous process (1), pedicle of vertebral arch (1), and an indeterminate location (1). Six patients presented with myelopathy from spinal cord compression and 8 with radiculopathy from nerve root compression. Operative approaches included posterior (11) and combined posterior anterior (3) patients. The surgical outcome was satisfactory in 79% (11) of patients. Two recurrent lesions were notable for sarcomatous transformation. CONCLUSIONS: We recommend gross total resection of all diagnosed OCs involving the mobile spine because of the risk of malignant transformation. Early detection and total excision of symptomatic spinal lesions in these cases may be the key to providing the best outcome. The neurological defect arising from OC can be improved by surgical intervention in most patients.

The circadian rhythm of arterial blood pressure in Alzheimer disease (AD) patients without hypertension.

OBJECTIVES: The circadian rhythm (CR) of arterial blood pressure (ABP) in Alzheimer disease (AD) patients was examined in a case-control clinical study. METHODS: This study was constructed using the case-control method and investigates non-hypertensive AD patients, compared with normotensive controls from a primary care setting. Twenty-four-hour ABP was measured with an automatic oscillometric device and recorded every 30 min throughout the day and night. Extreme dipper, dipper, non-dipper and reverse-dipper patterns were defined as those individuals with > 20%, 10-20%, < 10% and no fall in nocturnal ABP relative to daytime values. RESULTS: There were significant differences in ABP dipper status between cases and controls (cases - 16.15%, 60.00%, 17.70% and 6.15% vs controls - 3.19%, 31.9 2%, 42.02% and 22.88% for reverse dipper, non-dipper, dipper and extreme dipper, respectively, df = 3, χ(2) = 56.76, p < 0.001). Compared with normal controls, AD patients had significantly higher 24-h mean blood pressure, 24-h mean systolic blood pressure (SBP), night mean SBP, night mean pulse pressure (PP) and 24-h mean PP. There were no significant differences in 24-h mean diastolic blood pressure (DBP), daytime mean DBP or night-time mean DBP, and no significant differences in daytime mean SBP. CONCLUSIONS: The circadian rhythm of ABP in AD patents differed from normal controls, perhaps from higher night SBP in AD patents.

Cigarette smoking and its association with serum lipid/lipoprotein among Chinese nonagenarians/centenarians.

OBJECTIVE: Cigarette smoking had been confirmed as an increased risk for dyslipidemia, but none of the evidence was from long-lived population. In present study, we detected relationship between cigarette smoking habits and serum lipid/lipoprotein (serum Triglyceride (TG), Total cholesterol (TC), Low-density lipoprotein (LDL) and high-density lipoprotein (HDL)) among Chinese Nonagenarians/Centenarian. METHODS: The present study analyzed data from the survey that was conducted on all residents aged 90 years or more in a district, there were 2,311,709 inhabitants in 2005. Unpaired Student's t test, χ2 test, and multiple logistic regression were used to analyze datas. RESULTS: The individuals included in the statistical analysis were 216 men and 445 women. Current smokers had lower level of TC (4.05 ± 0.81 vs. 4.21 ± 0.87, t = 2.403, P = 0.017) and lower prevalence of hypercholesteremia (9.62% vs. 15.13%, χ2 = 3.018,P = 0.049) than nonsmokers. Unadjusted and adjusted multiple logistic regressions showed that cigarette smoking was not associated with risk for abnormal serum lipid/lipoprotein. CONCLUSIONS: In summary, we found that among Chinese nonagenarians/centenarians, cigarette smoking habits were not associated with increased risk for dyslipidemia, which was different from the association of smoking habits with dyslipidemia in general population.

Association between sleep quality and cognitive impairment among Chinese nonagenarians/centenarians.

PURPOSE: The authors examined the individual association between sleep quality and cognitive function among the very elderly. METHODS: The present study analyzed data from a survey that was conducted on all residents aged 90 years or older in a district; there were 2,311,709 inhabitants in 2005. Cognitive function and sleep quality were measured using the 30-item Mini-Mental State Examination and the Pittsburgh Sleep Quality Index, respectively. RESULTS: The subjects included in the statistical analysis were 216 men and 444 women. Subjects with poor sleep quality had significant lower cognitive function scores (P = 0.007) and higher prevalence of cognitive impairment (P = 0.035). Subjects with cognitive impairment had longer sleep latency (P < 0.0001) and lower sleep efficiency percentage (P = 0.006) and more likely reported with poor quality (P = 0.035). Multiple logistic regressions showed that poor sleep quality was a risk factor for cognitive impairment (unadjusted odds ratio, 1.719; 95% confidence interval, 1.138-2.597; adjusted odds ratio, 1.759; 95% confidence interval, 1.012-3.057). CONCLUSIONS: Among longevity Chinese, there is an association between sleep quality and cognitive function, and cognitive impairment is associated with poor quality, longer sleep latency, and lower sleep efficiency percentage.

Living arrangements and risk for late life depression: a meta-analysis of published literature.

OBJECTIVE: The goal of this study was to determine the relationship between living arrangements and risk for depression among older people. METHOD: MEDLINE, EMBASE, The Cochrane Library database was used to identify potential studies. The studies were divided into cross-sectional and longitudinal subsets. Qualitative meta-analysis of the cross-sectional studies and longitudinal studies was performed, respectively. For prevalence and incidence rates of depression, odds ratio (OR) and relative risk (RR) were calculated, respectively. RESULTS: The qualitative meta-analysis showed that older people living alone had a higher risk of depression than those not living alone (OR: 1.44; 95% confidence interval [95% CI]: 1.04-1.99); Relative risk (RR: 1.27, 95% CI: 0.89-1.80) and those living with families (OR: 2.59, 95% CI: 1.60-4.20). Older people living in a nursing home (OR: 2.90, 95% CI: 0.94-8.94; RR: 1.94, 95% CI: 1.18-3.20) or institutionalized setting (OR: 1.86, 95% CI: 1.37-2.52; RR: 2.03, 95% CI: 1.12-3.70) had a higher risk of depression than those living in home. CONCLUSIONS: Despite the methodological limitations of this meta-analysis, living arrangements appear related to the risk for depression in the older population. Older persons living alone, in a nursing home, or in an institutionalized setting have higher risk for depression.

Association between sleep quality and arterial blood pressure among Chinese nonagenarians/centenarians.

BACKGROUND: There is association between sleep quality and arterial blood pressure, but it is still unclear if the association also exists in the very elderly. We examined the individual association between sleep quality and arterial blood pressure among the very elderly. MATERIAL/METHODS: The present study analyzed data from a survey that was conducted on all residents aged 90 years or older in a district with 2,311,709 inhabitants in 2005. Sleep quality was measured using The Pittsburgh Sleep Quality Index (PSQI). RESULTS: The subjects included in the statistical analysis were 216 men and 444 women. There were no significant differences in sleep quality scores, sleep latency, and sleep efficiency percentage and prevalence of poor sleep quality between subjects with and without hypertension. None of the differences in systolic blood pressure, diastolic blood pressure, and prevalence of hypertension, systolic hypertension and diastolic hypertension among subjects with well, fairly and poor sleep quality were significant. Multiple logistic regressions showed that unadjusted and adjusted Odds Ratio (ORs) of poor sleep quality for increased risk for hypertension were significant. CONCLUSIONS: Among very elderly subjects, there was no association between sleep quality and arterial blood pressure.

Association between sleep quality and body mass index among Chinese nonagenarians/centenarians.

We examined the individual association between body mass index (BMI) and sleep quality among the very elderly. The present study analyzed data from survey that was conducted on all residents aged 90 years or more in a district, there were 2,311,709 inhabitants in 2005. Subjects were divided into four groups according to quartile of BMI (<16.6, 16.6-18.9, 18.9-21.1, >21.1 kg/m(2)) and according to classification criteria of underweight, normal weight, overweight, and obesity in BMI (<18.5, 18.5-23.0, 23.0-27.5, >27.5 kg/m(2)), respectively. Sleep quality was measured using The Pittsburgh Sleep Quality Index (PSQI). Sleep quality included quality classification and scores, sleep duration, sleep latency, and sleep efficiency. The subjects included in the statistical analysis were 216 men and 444 women. According to quartile of BMI or classification criteria of underweight, normal weight, overweight, and obesity in BMI, none of the differences in sleep quality scores, sleep latency, sleep duration, sleep efficiency percentage, and prevalence of poor sleep quality was significant among different BMI groups. The difference in BMI between subjects with good and poor sleep quality was non-significant. Unadjusted and adjusted multiple logistic regression showed that none of the BMI groups had a function of decreasing the risk for poor quality. Among longevity Chinese, there is no association between BMI and sleep quality.

Association of serum lipid/lipoprotein with Pro12Ala polymorphism in PPAR-γ2 among Chinese nonagenarians/centenarians.

BACKGROUND AND AIMS: In previous studies, the Pro12Ala polymorphism in the peroxisome proliferator-activated receptor gamma 2 (PPAR-γ2) was shown to be associated with both lipid metabolism and longevity. We examined whether the polymorphism continued to be associated with abnormal levels of serum lipid/lipoprotein among elderly subjects (≥90 years). METHODS: The Pro12Ala variant was examined using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Abnormal levels of serum lipid/lipoprotein were defined according to the criteria provided by the Chinese Medical Association (2004). Abnormal criteria were triglyceride (TG) >5.18 mmol/l, total cholesterol (TC) >1.7 mmol/l, low-density lipoprotein cholesterol (LDL-C) >3.37 mmol/l and high-density lipoprotein cholesterol (HDL-C) <1.04 mmol/l). RESULTS: The sample included 673 unrelated Chinese individuals aged 90-108 years (mean age: 93.54 ± 3.54 years) and 67.3% females. Genotype frequencies of the Pro12Ala polymorphism were 0% Ala12Ala, 8.9% Pro12Ala, 91.1% Pro12Pro. Neither differences in the levels of serum lipid/lipoprotein nor the prevalence of their abnormal levels was significant between subjects who were or were not 12Ala carriers. Unadjusted and adjusted multiple logistic regressions showed that the odds ratios (OR) for abnormal levels of serum lipid/lipoprotein were not associated with the Pro12Ala polymorphism in PPAR-γ2. CONCLUSIONS: Levels of serum lipid/lipoprotein were not associated with the Pro12Ala polymorphism in PPAR-γ2 among Chinese nonagenarians and centenarians, which was different from the general population.

Serum lipid/lipoprotein and arterial blood pressure among Chinese nonagenarians/centenarians.

OBJECTIVE: In the present study, we detected a relationship between serum lipid/lipoprotein [serum triglyceride (TG), total cholesterol (TC), low-density lipoprotein (LDL)-cholesterol and high-density lipoprotein (HDL)-cholesterol] and arterial blood pressure among Chinese nonagenarians/centenarian. METHODS: The present study analyzed data from the survey that was conducted on all residents aged 90 years or more in a district; there were 2,311,709 inhabitants in 2005. Arterial blood pressure included systolic blood pressure (SBP) and diastolic blood pressure (DBP). RESULTS: The subjects included in the statistical analysis were 217 men and 444 women. Subjects with hypertension (1.29 ± 0.74 vs 1.13 ± 0.45, t = 3.362, p = 0.001) or systolic hypertension (1.30 ± 0.74 vs 1.12 ± 0.45, t = 3.534, p < 0.0001) had higher levels of TG than those without. Subjects with abnormal levels of serum TG had higher SBP (145 ± 22 vs 139 ± 23, t = 2.223, p = 0.027). The Pearson correlation showed a significant relationship between SBP and serum TG levels (r = 0.088, p = 0.024). Unadjusted and adjusted multiple logistic regressions showed that hypertension or systolic hypertension was associated with an increased risk of abnormal of serum TG levels. CONCLUSIONS: In summary, we found that among Chinese nonagenarians and centenarians, the levels of serum lipid/lipoprotein were associated with arterial blood pressure. Hypertriglyceridemia was associated with SBP.