Unveiling the hidden threat of microplastics to coral reefs in remote South Atlantic islands.

The widespread presence of marine microplastics (< 5 mm) is a significant concern, as it may harm marine biodiversity and ocean ecosystems. Corals' capacity to ingest microplastics has emerged as a significant threat to reef ecosystems, owing to the detrimental physiological and ecological effects it can trigger. The extent of the impact of microplastics on Brazilian corals remains unclear and this study aimed to investigate its distribution and characteristics in four coral species: Favia gravida, Mussismilia hispida, Montastrea cavernosa, and Siderastrea stellata, found in the Trindade and Martim Vaz Islands - the most isolated archipelago of Brazil, located about 1200 km (680 miles) east of the coast. This study aims to reveal the extent of microplastic distribution in the coral reef environment, assess the amount of microplastics in different coral species, and compare each species' capacity to adhere and accumulate microplastics. A high concentration of ingested and adhered microplastics was detected in all coral species evaluated in the present study. No significant differences were observed in the sampling points which indicates that although the sampling points are located at different distances from the coast, the microplastic pollution is equally distributed in the region. Polyethylene (PE), polyvinyl chloride (PVC), polypropylene (PP), poly(methyl methacrylate) (PMMA), Rayon, and Nylon particles were detected, with a predominance of PE (45.5 %). No significant differences in microplastic concentration were detected among the various species and locations studied. Our research presents findings that demonstrate the extensive occurrence of microplastic contamination in coral colonies located on remote islands.

Exposure of the amphipod Hyalella azteca to microplastics. A study on subtoxic responses and particle biofragmentation.

Microplastics are widespread pollutants in the environment and are considered a global pollution problem. Microplastics mostly originate from larger plastics and due to environmental conditions are undergoing constant fragmentation processes. It is important to understand the fragmentation pathways, since they play a key role in the fate of the particles, and also directly influence toxicity. Amphipods are potential inducers of plastic debris fragmentation. Here, Hyalella azteca was exposed to different concentrations (540, 2700, 5400 items/L) of 24.5 µm polystyrene microplastics (PS-MP) for 7 days. After exposure, oxidative stress, particle size reduction, and mortality were checked. No significant mortality was seen in any of the treatments, although changes were recorded in all enzymatic biomarkers analyzed. It was observed that throughout the ingestion and egestion of PS-MP by H. azteca, particles underwent intense fragmentation, presenting a final size up to 25.3% smaller than the initial size. The fragmentation over time (24, 72, 120, 168 h) was verified and the results showed a constant reduction in average particle size indicating that H. azteca are able to induce PS-MP fragmentation. This process may facilitate bioaccumulation and trophic particle transfer.

Biological responses of Chironomus sancticaroli to exposure to naturally aged PP microplastics under realistic concentrations.

Microplastic (MP) is yet another form of chronic anthropogenic contribution to the environment. MPs are plastic particles (<5 mm) that have been widely found in the most diverse natural environments, but their real impacts on ecosystems are still under investigation. Here, we studied the toxicity of naturally aged secondary polypropylene (PP) MPs after constant exposure to ultraviolet radiation (26 µm) to the third instar larvae of Chironomus sancticaroli, a dipteran species. The concentrations tested were 13.5; 67.5; and 135 items g-1 of dry sediment. C. sancticaroli organisms were investigated for fragment ingestion, mortality and changes in enzymatic biomarkers after 144 h of exposure. The organisms were able to ingest MPs from the first 48 h, and the amount of items internalized was dose-dependent and time-dependent. Overall, the results show that mortality was low, being significant at the lowest and highest concentrations (13.5 and 135 items g-1). Regarding changes in biochemical markers, after 144 h MDA and CAT activities were both significantly altered (increased and reduced, respectively), while SOD and GST levels were unchanged. In the present study, naturally aged polypropylene MPs induced biochemical toxicity in C. sancticaroli larvae, with toxicity being higher according to exposure time and particle concentration.

Toxicological effects caused by environmental relevant concentrations of ketoconazole in Chironomus sancticaroli (Diptera, Chironomidae) larvae evaluated by oxidative stress biomarkers.

Ketoconazole (KTZ), a broad-spectrum fungicidal drug, has been a significant problem in recent decades due to its toxic action on non-target aquatic organisms. Thus, the present study aimed to evaluate determine the effects that environmental relevant concentration of the commercial formulation of KTZ can exert on benthic macroinvertebrates, more specifically on larvae of the insect Chironomus sancticaroli. Acute toxicity tests with KTZ indicated lethal concentration (LC50) of 9.9 µg/L. Analyses of prolonged exposure to KTZ (chronic toxicity) indicated an increase in the rate of mentum deformity by approximately 3 times at concentrations of 0.6 and 2.4 µg/L. All biomarkers analyzed showed an increase after exposure to KTZ (0.6 and 2.4 µg/L), with average values of 115 % for superoxide dismutase (SOD), 63 % for catalase (CAT), 111 % for glutathione S-transferase (GST) and 59 % for malonaldehyde (MDA) in C. sancticaroli larvae. Thus, the toxic effects on survival, development (length and weight), mentum and redox responses caused by commercial KTZ in low concentrations were observed on C. sancticaroli larvae. In addition, the results suggest that biochemical biomarkers can be used for studies involving environmental disturbances.

Ecological risk of imidacloprid on the Brazilian non-target freshwater organisms Chironomus sancticaroli and Poecilia reticulata.

Imidacloprid (IMI) is a neonicotinoid insecticide widely used in agriculture worldwide. This pesticide has been found in freshwater ecosystems, including Brazilian freshwaters. For this reason, studies are being conducted to detect the presence of IMI in freshwater and understand its effects on the aquatic biota. In the present study, the acute toxic effect of the imidacloprid commercial formulation (ICF) Galeão® on the Brazilian non-target aquatic organisms Chironomus sancticaroli and Poecilia reticulata was evaluated. Enzymatic activities (glutathione S-transferase (GST), catalase (CAT), and ascorbate peroxidase (APX)) were also determined. Moreover, we considered 11 studies that detected IMI concentrations up to 3.65 µg.L-1 in 28 different Brazilian freshwaters to evaluate the acute ecological risk of IMI in these environments. From the ecotoxicological assays, we determined the LC50 values for C. sancticaroli (LC50-48 h 1.52 µg.L-1) and P. reticulata (LC50-96 h 122.65 mg.L-1). The high sensitivity of C. sancticaroli demonstrates that this species could be used as a bioindicator in studies investigating the contamination of freshwater by IMI. Enzymatic activity changes were observed in both organisms and offered sublethal responses to the effects of the pollution by IMI on aquatic biota. Our results suggest that the presence of IMI in Brazilian aquatic ecosystems can represent a potential ecological risk for the aquatic insect populations and, consequently, cause an imbalance in these ecosystems. The present study provides relevant and comparable toxicity information that may be useful to develop public policies to protect the Brazilian aquatic ecosystem from IMI contamination.

Ecotoxicological effect of ketoconazole on the antioxidant system of Daphnia similis.

The occurrence of emerging pharmaceutical pollutants (i.e. small drugs, antibiotics) present in aquatic environments shown to be a current environmental problem still without apparent solution. In this regard, the use of ecotoxicological techniques has been shown fundamental for the appraisal of damage to affected living organisms. Herein, ecotoxicological tests were conducted, focusing on the evaluation of the effects of ketoconazole (KTZ) on the antioxidant system of the model body Daphnia similis. In order to study the biochemical changes caused by KTZ in the antioxidant system, the enzymatic biomarkers glutathione S-transferase (GST), catalase (CAT), and ascorbate peroxidase (APX) were monitored. Toxicological tests were conducted using KTZ concentrations (0-10 µg·L-1). Prolonged exposure to KTZ (336 h) caused changes upon the expression of antioxidant enzymes and simultaneously affected the reproductive system in those organisms. Moreover, a decrease in GST and APX activity was observed caused by KTZ exposure, respectively 79.2% (3.53 µmol min-1 mg-1 protein) and 24.4% (0.88 µmol min-1 mg-1 protein). On the other hand, it was observed an increase of 27% (0.17 µmol min-1 mg-1 protein) in CAT activity. Through this study, it was possible to observe the toxicological effects of KTZ, which proves its action as an oxidative stress-inducing agent and endocrine modifier in daphnids organisms.

Responses of Aquatic Nontarget Organisms in Experiments Simulating a Scenario of Contamination by Imidacloprid in a Freshwater Environment.

Several studies have indicated the presence of the neonicotinoid insecticide imidacloprid (IMI) in aquatic ecosystems in concentrations up to 320.0 µg L-1. In the present study, we evaluated the effects of the highest IMI concentration detected in surface water (320.0 µg L-1) on the survival of Chironomus sancticaroli, Daphnia similis, and Danio rerio in three different scenarios of water contamination. The enzymatic activities of glutathione S-transferase (GST), catalase (CAT), and ascorbate peroxidase (APX) in D. rerio also were determined. For this evaluation, we have simulated a lotic environment using an indoor system of artificial channels developed for the present study. In this system, three scenarios of contamination by IMI (320.0 µg L-1) were reproduced: one using reconstituted water (RW) and the other two using water samples collected in unpolluted (UW) and polluted (DW) areas of a river. The results indicated that the tested concentration was not able to cause mortality in D. similis and D. rerio in any proposed treatment (RW, UW, and DW). However, C. sancticaroli showed 100% of mortality in the presence of IMI in the three proposed treatments, demonstrating its potential to impact the community of aquatic nontarget insects negatively. Low IMI concentrations did not offer risks to D. rerio survival. However, we observed alterations in GST, CAT, and APX activities in treatments that used IMI and water with no evidence of pollution (i.e., RW and UW). These last results demonstrated that fish are more susceptible to the effects of IMI in unpolluted environments.

Parasite detection in sand from bays on the north coast of São Paulo state, Brazil

Soil contamination by protozoan parasites and soil-transmitted helminths (STH) is common in beach sand due to a number of factors such as pets, pluvial water, garbage, etc. These pathogens may cause many diseases in humans and animals and become a public health problem. Thus, systematic evaluation and inspection are necessary to develop control strategies regarding public contamination. For this purpose, our aims were to evaluate the parasitic profile of sandy soils on an urban beach and an untouched beach and correlate this with environmental and seasonal characteristics in Ubatuba, Brazil, in two seasons (winter and summer). 132 soil samples were collected for parasite analysis utilizing Rugai's method and the sedimentation adapted method. Our results showed positivity in 62% of the samples for at least one parasite in the urban beach and no parasitic structures on the untouched beach. The positivity was higher in summer (85%) than in winter (51.7%). All samples were positive for both, helminths and protozoa. Seasonal influence was noted regarding the presence of STH while for protozoa this influence was not observed. The parasitic structures most found were larvae of hookworms (35%) and Toxocara eggs (31.7%). We also noted the presence of Strongyloides sp, Ascaris lumbricoides, coccidia, Dipylidium caninum, Entamoeba hystolitica/dispar, Endolimax nana, Entamoeba coli, Giardia sp, Toxascaris leonina, Trichuris sp and Dibothricephalus latus. There was a positive correlation between temperature and the presence of STH in the sand samples. Most of the collection sites on the urban beach presented dogs or canine traces and garbage in both seasons. There was an association between the presence of dogs or their traces and parasitic structures. In conclusion, seasonality, urbanization and the presence of pets on beaches can potentially favor environmental contamination by parasites increasing the risk of transmission of zoonotic and parasitic diseases.

Avaliação da eficiência de um índice de estado trófico na determinação da qualidade da água de reservatórios para abastecimento público / Assessment of the efficiency of a trophic state index in determining the water quality of public water supply reservoirs

RESUMO O objetivo deste estudo consiste na avaliação da eficácia do uso de um índice de estado trófico (IET) como ferramenta na análise da qualidade das águas de três reservatórios. O IET foi calculado baseado na transparência do reservatório e nas concentrações de clorofila-a e fósforo total (P total). Para identificar a correlação do uso do solo com a poluição aquática das represas, o IET calculado para cada represa foi comparado ao mapeamento temático de cobertura do solo com base em imagens do satélite Landsat TM 5 do ano de 2010. A análise dos componentes principais (ACP), focada na correlação dos dados, foi realizada utilizando o software PAST®, versão 2.16. Inicialmente, o comportamento dos parâmetros associados ao IET foi avaliado de forma isolada (P total, clorofila-a e transparência) sobre os reservatórios. Em seguida, foram aplicados à ACP os demais parâmetros físicos e químicos associados à qualidade da água dos reservatórios (sem o IET). Por fim, todos os parâmetros foram submetidos à ACP (IET e todos os outros parâmetros em conjunção). O IET utilizado nesta pesquisa demonstrou ser autossuficiente na predição da qualidade de água com a utilização de apenas três parâmetros (P total, clorofila-a e transparência). A análise das imagens de satélite, em conjunto com os dados provenientes do IET, foi suficiente para apontar a redução da qualidade das águas por fontes pontuais de poluição, mesmo em ambientes com maior cobertura vegetal preservada.

ABSTRACT The aim of this study consists in the evaluation of the trophic state index (IET) efficacy as a tool in the water quality analysis of three reservoirs. The IET was calculated based on water transparency in the reservoir and on the surface concentrations of chlorophyll-a and total phosphorus (total P). To identify the correlation of the soil use with the reservoir aquatic pollution, the IET calculated for each reservoir was compared to the thematic mapping of land cover based on Landsat TM 5 satellite images from 2010. The main components analysis (ACP), focused on data correlation, was made using the software PAST®, version 2.16. Initially, the behavior of the parameters associated with the IET was evaluated alone (total P, chlorophyll-a and transparency) on the reservoirs. Then, the other chemical and physical parameters associated with the reservoirs water quality (without the IET) were applied to the ACP. Finally, all the parameters underwent the ACP (IET and all the other parameters in conjunction). The IET used in this research seemed to be self-sufficient in the prediction of water quality using only three parameters (total P, chlorophyll-a and transparency). The satellite images analysis, in conjunction with the IET data, was sufficient to point out the water quality reduction by punctual sources of pollution even in environments with larger preserved vegetal coverage.