RESUMO
Each of the serological methods used for the screening of T. cruzi carriers has its advantage and disadvantage. Owing to the detection, particularly concerning blood transfusion, a selection of techniques, as wide as possible, and which don't aim the same antibodies is now carried out. Therefore, we have tried to perfect an ELISA-like reaction. This kind of analysis appeared very sensitive, faithful and reproducible in other parasitology fields. It became evident for us to try to apply this method to the diagnosis of Chagas disease. In order to test this method , we compared the results obtained with the one given by other usual reactions: immunofluorescence and passive hemagglutination test. In an other hand, we have, for the whole three methods, estimated the possible crossed reactions, particularly with leishmaniasis, african trypanosomiasis and myeloma. From these results, it can be deduced that: --the ELISA reaction used with a total homologous antigen is sensitive and discriminant towards the other trypanosoma. The ratio of the crossed reactions with visceral leishmaniasis is the same compared to immunofluorescence. It allows the exploration of recent infestations; --the ELISA reaction done with a dislipided antigen is very sensitive. In the meantime, crossed reactions are possible with other trypanosomiasis.