RESUMO
The tube leukocyte adherence inhibition (LAI) assay was performed using 3 M KCl extracts of malignant melanoma materials and leukocytes taken from 24 malignant melanoma patients. Of these patients, 21 participated in sequential studies which employed a minimum of 3 time points. An increasing non-adherence index (NAI) was found in 5 of 6 patients who exhibited a favorable disease course. In 5 of 6 patients with an unfavorable disease course, NAI values decreased. In 8 of 9 patients with stable disease, the NAI also was stable. These results indicate that the trend of sequential LAI studies reflects the clinical course of malignant melanoma patients.
Assuntos
Técnicas Imunológicas , Teste de Inibição de Aderência Leucocítica , Leucócitos/imunologia , Melanoma/imunologia , Feminino , Humanos , Masculino , Melanoma/patologia , Pessoa de Meia-Idade , Cloreto de PotássioRESUMO
Leukocyte adherence inhibition (LAI) assays were performed to test whether peripheral blood leukocytes (PBL) from patients with colorectal cancer could be inhibited from attachment to a glass surface when tumor-associated antigens (TAA) of human colorectal tumors were present. The assays were performed with PBL from 41 patients with adenocarcinoma of the colon or rectum with 3-M KCl extracts of colorectal tumors. The results demonstrated the presence of colorectal TAA in the 3-M KCl extract of colorectal tumor materials. The reactivity of leukocytes from patients with a favorable prognosis showed an increasing LAI trend; the reactivity of leukocytes from patients with an unfavorable prognosis had a decreasing LAI trend. In individual patients, alterations in the sequential LAI results paralleled changes in the clinical status, which thus strongly indicated that the LAI assay could be of value in the assessment of antitumor immunity.
Assuntos
Adenocarcinoma/imunologia , Antígenos de Neoplasias , Neoplasias do Colo/imunologia , Técnicas Imunológicas , Teste de Inibição de Aderência Leucocítica , Leucócitos/imunologia , Neoplasias Retais/imunologia , Adulto , Idoso , Antígenos de Neoplasias/isolamento & purificação , Feminino , Humanos , Imunidade Celular , Técnicas In Vitro , Masculino , Pessoa de Meia-Idade , Cloreto de Potássio , Prognóstico , Fatores de TempoRESUMO
Erythrocytic survival and ferrokinetic studies were carried out in dogs with cyclic hematopoiesis. The serum iron values varied during different phases of the cycle. The highest values occurred when the bone marrow predominantly contained granulocytic cells. The erythrocytic survival and the remainder of the ferrokinetic variables were comparable to those in normal dogs of a similar age.
Assuntos
Agranulocitose/veterinária , Eritrócitos/fisiologia , Eritropoese , Neutropenia/veterinária , Anemia/sangue , Anemia/veterinária , Animais , Sobrevivência Celular , Ferro/sangue , Neutropenia/sangueRESUMO
Anti-erythropoietin antibodies (anti-ESF) were determined by the capacity of the immunoglobulins to neutralize the biological activity of erythropoietin (ESF). Affinity chromatographic methods were used to initiate the purification of anti-ESF by use of ESF covalently linked to Sepharose. It was observed that anti-ESF bound the ESF-Sepharose. In order to remove the nonspecific immunoglobulins, the anti-ESF preparation was subjected to further chromatography on affinity colums where either serum or urinary proteins from a polycythemic patient were covalently linked to Sepharose. It was demonstrated the affinity chromatography could be used as a method isolate anti-ESF.