Bioavailability and toxicity to rats of bound residues of 14C-pirimiphos-methyl in stored wheat.

Wheat grains were treated with 14C-pirimiphos-methyl to generate bound residues for testing their bioavailability to rats. Bound residues accounted for 25% of the applied dose (50 ppm) at the end of one year. When the grain bound residues were fed to rats for 48 hours the animals eliminated 30 and 40% of the administered dose in urine and feces respectively, after 5 days. Radioactivity in some selected organs and blood accounted for 37% of the administered dose after 2 days, which gradually declined to 1% after 5 days. These data indicate that wheat-bound pirimiphos-methyl residues are moderately bioavailable to rats. In a 90-day feeding study, inhibition of plasma cholinesterase and brain acetylcholinesterase strongly suggest that the bound residues possess a toxicological potential.

Preliminary screening of amino acids excretion in the urine of newly born babies.

The paper attempts to characterize the qualitative as well as quantitative estimations of aminoacids in the urines of new born babies using two dimensional paper chromatography. The objective of this investigation was to know the extent of amino acid defects in these samples and to correlate such defects with the physical abnormalities in infants. Based on the overall results, it is inferred that phenylalanine excreted in the urine gave a positive response with PKU test and some particular amino acids are identified and estimated in the urine of new born babies to confirm the diagnosis of phenylketonuric patients.