Machine learning techniques for periodontitis and dental caries detection: A narrative review.

OBJECTIVES: In recent years, periodontitis, and dental caries have become common in humans and need to be diagnosed in the early stage to prevent severe complications and tooth loss. These dental issues are diagnosed by visual inspection, measuring pocket probing depth, and radiographs findings from experienced dentists. Though a glut of machine learning (ML) algorithms has been proposed for the automated detection of periodontitis, and dental caries, determining which ML techniques are suitable for clinical practice remains under debate. This review aims to identify the research challenges by analyzing the limitations of current methods and how to address these to obtain robust systems suitable for clinical use or point-of-care testing. METHODS: An extensive search of the literature published from 2015 to 2022 written in English, related to the subject of study was sought by searching the electronic databases: PubMed, Institute of Electrical and Electronics Engineers (IEEE) Xplore, and ScienceDirect. RESULTS: The initial electronic search yielded 1743 titles, and 55 studies were eventually included based on the selection criteria adopted in this review. Studies selected were on ML applications for the automatic detection of periodontitis and dental caries and related dental issues: Apical lessons, Periodontal bone loss, and Vertical root fracture. CONCLUSION: While most of the ML-based studies use radiograph images for the detection of periodontitis and dental caries, few pieces of the literature revealed that good diagnostic accuracy could be achieved by training the ML model even with mobile photos representing the images of dental issues. Nowadays smartphones are used in every sector for different applications. Training the ML model with as many images of dental issues captured by the smartphone can achieve good accuracy, reduce the cost of clinical diagnosis, and provide user interaction.

Spectrum of Neurological Manifestations of COVID-19 Data from a Tertiary Care Hospital.

Background: Coronavirus is a novel virus which has disrupted life in the past year. While it involves the lungs in the majority and this has been extensively studied, it involves other organ systems. More number of studies need to be focused on the extrapulmonary manifestations of the disease. Objective: To delineate the clinical manifestations of coronavirus disease 2019 (COVID-19) virus on the central and peripheral nervous systems and to assess the risk factors and the outcome of COVID-19 patients with neurological manifestations. Materials and Methods: All patients who were SARS-CoV-2 RNA polymerase chain reaction (PCR) positive were assessed, and detailed clinical history and laboratory findings were collected. Data was analyzed using percentage, mean, and frequency. Results: Out of 864 patients, 17 (N = 17, 1.96%) had neurological manifestations. Twelve out of 17 had comorbid conditions. Patients had diverse presentations ranging from acute cerebrovascular accident to paraplegia and encephalopathy. Ten (58.8%) patients presented with acute cerebrovascular accidents. Of the patients who developed stroke, five (50%) died. Conclusions: COVID-19 usually presents as a respiratory disease. The neurological manifestations of COVID-19 are not uncommon. One should be aware of a wide spectrum of neurological signs and symptoms of COVID-19 for early diagnosis and treatment for preventing mortality and morbidity.

Cyclic mood disorder heralding adult-onset autosomal dominant leucodystrophy: a clinical masquerader.

Leucodystrophies are a heterogeneous group of progressive white matter diseases which may be inherited in dominant, recessive or X-linked fashion depending on the type. Adrenoleucodystrophy (ALD) and metachromatic leucodystrophy (MLD) are rather commoner forms of leucodystrophies whereas krabbes disease, alexander disease, cannavans disease etc. are of less common type. Adult-onset autosomal dominant leucodystrophy (ADLD) is a lately described rarer form of leucodystrophy with perhaps no case report from India. Various leucodystrophies may have different clinical presentations, ranging from subtle cognitive and psychiatric manifestations to gross motor disabilities, visual impairment and seizure. Psychiatric manifestations in the form of psychoses and frank schizophrenia are commonly described in MLD. Depression though uncommonly reported in MLD, cyclic mood disorders have been rarely described in any form of leucodystrophies. We are reporting an eye opener, a case of ADLD which masqueraded as a rapid cyclic mood disorder for initial four years, later to be followed by progressive neurological signs and symptoms. To the best of our knowledge, this is perhaps the first case report of ADLD presenting as rapid cyclic mood disorder in the world literature.

Larvicidal, ovicidal, and oviposition-deterrent activities of four plant extracts against three mosquito species.

In mosquito control programs, insecticides of botanical origin have the potential to eliminate eggs, larvae, and adults. So, the larvicidal, ovicidal, and oviposition-deterrent activities of petroleum ether and ethyl acetate extracts of the leaves of Eugenia jambolana, Solidago canadensis, Euodia ridleyi, and Spilanthes mauritiana were assayed against the three vector mosquito species, namely Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus. The larval bioassay was conducted following the World Health Organization method. The maximum larval mortality was found with ethyl acetate extract of S. mauritiana against the larvae of A. stephensi, A. aegypti, and C. quinquefasciatus with LC50 values of 11.51, 28.1, 14.10 ppm, respectively. The mean percent hatchability of the ovicidal activity was observed at 48-h post-treatment. The percent hatchability was found to be inversely proportional to the concentration of the extract and directly proportional to the number of eggs. The flower head extract of S. mauritiana gave 100% mortality followed by E. ridleyi, S. canadensis, and E. jambolana against the eggs of the three mosquito vectors. For oviposition-deterrent effect, out of the five concentrations tested (20, 40, 60, 80, and 100 ppm), the concentration of 100 ppm showed a significant egg laying-deterrent capacity. The oviposition activity index value of E. jambolana, E. ridleyi, S. canadensis, and S. mauritiana against A. aegypti, A. stephensi, C. quinquefasciatus at 100 ppm were -0.71, -0.71, -0.90, -0.93, -0.85, -0.91, -1, -1, -0.71, -0.85, -1, and -1, respectively. These results suggest that the leaf/flower extracts of certain local plants have the potential to be developed as possible eco-friendly means for the control of mosquitoes.

Hereditary amyloid polyneuropathy in a family from North West India: phenotypic, MRI and pathologic study.

Hereditary amyloid polyneuropathies are rare, heterogeneous group of autosomal dominant disorders and deserve special attention because of its rare presentation, multisystem involvement and significant therapeutic implications if diagnosed early. We report a male patient of hereditary amyloid polyneuropathy from North West India with peripheral nerve, autonomic nervous system, vitreous and cardiac involvement.

Rapid thrombosis of middle cerebral artery aneurysm after subarachnoid haemmorhage.

Spontaneous thrombosis of intracranial aneurysm is a rare event but is frequent after subarachnoid haemorrhage (SAH) and in fusiform or giant saccular aneurysms. We report a case of a 20-year-old man presenting with SAH due to rupture of a giant aneurysm of the middle cerebral artery. Initial CT angiography (CTA) revealed partially thrombosed MCA aneurysm but digital subtraction angiography performed 3 days later revealed complete occlusion of the aneurysm. Rapid thrombosis of aneurysm within 3 days has not been reported in literature so far.

Synergistic effect of Eugenia jambolana Linn. and Solidago canadensis Linn. leaf extracts with deltamethrin against the dengue vector Aedes aegypti Linn. at Mysore.

With the goal in mind to minimize the application of environmentally hazardous chemical insecticides, the larvicidal activity of two plant extracts along with deltamethrin was studied at University of Mysore. The extracts of Solidago canadensis and Eugenia jambolana were employed for working out the synergistic efficacy against Aedes aegypti larvae, as the extracts of both the plants exhibited high efficacy when applied individually. The deltamethrin when analyzed separately, LC50 and LC90 values were 0.00045 and 0.00148 ppm, respectively. Synergistic studies with two plant extracts on deltamethrin revealed S. canadensis as more effective with synergistic factor(SF) of 4.090 for LC50 value and 4.781 for LC90 followed by E. jambolana with SF 1.80 for LC50 and 2.467 for LC90 at 1:1 ratio of the phytoextracts and deltamethrin. Thus, S. canadensis was found to be a better larvicidal and synergistic agent. Combination of phytochemical and insecticide were found to be more effective than insecticides or phytochemicals alone which could be a good ecofriendly and cost-effective approach to reduce the dose of chemicals with high residual effect to be applied in vector control programs.

Effect of dietary energy on seminal plasma insulin-like growth factor-I (IGF-I), serum IGF-I and testosterone levels, semen quality and fertility in adult rams.

The objective of the present study was to modulate seminal plasma insulin-like growth factor-I (IGF-I) by dietary energy and assess the relationship among testosterone and IGF-I levels, semen quality and fertility in adult rams. Twenty-four 1-yr old adult Nellore rams were equally divided into three groups (n = 8) and fed with three different concentrate mixtures formulated using conventional ingredients and finger millet (Eleucine corocana) straw to ensure rams received with similar amount of crude protein with three levels of energy. Rams in low-energy group were offered diets with 20% less energy than the control energy group (optimum energy, 100%, recommended energy level), whereas rams in high energy group were offered diets with 20% more energy than the optimum energy group. Semen was collected from rams 60 days after start of the experimental feeding. The percentages of progressive forward motility, functional membrane integrity and mitochondrial membrane potential of the spermatozoa were significantly (P < 0.05) higher in control and high energy groups as compared to low-energy group. Feeding of low-energy diet significantly (P < 0.05) decreased spermatozoa VSL, VCL and VAP when compared to control and high energy fed groups. The number of spermatozoa binding/oocyte was significantly (P < 0.05) higher in control (11.23 ± 0.20) and high energy (10.57 ± 0.19) groups as compared to the low energy (6.14 ± 0.01) group. The serum and seminal plasma IGF-I levels were significantly (P < 0.05) higher in control and high energy fed groups as compared to the low-energy group. The serum testosterone and cholesterol levels were significantly (P < 0.05) higher in the control group as compared to the low-energy group. The seminal plasma fructose levels in optimum energy fed animals were significantly (P < 0.05) higher as compared to other two groups. The seminal plasma IGF-I level had positive correlation with progressive forward motility (r = 0.7) and other velocity (linearity, r = 0.7; straightness, r = 0.7) parameters. The study suggested that the modulation of seminal plasma IGF-I levels by dietary energy is possible and the optimum level of seminal plasma IGF-I is necessary and sufficient to influence semen quality.

Detection of follicular apoptosis in water buffalo (Bubalus bubalis) ovary by histology and nick end labelling technique.

The objective of this experiment was to assess the features and extent of follicular apoptosis in the water buffalo (Bubalus bubalis) ovary using classical histology and nick end labelling technique. Ovaries (n=40) procured from the slaughterhouse were used for the study. The sections (5 µm) were used for detection of terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling (TUNEL) and classical histology (H&E). Those follicles showing ≥ 5% TUNEL positivity (TUNEL assay) and pyknotic nuclei (histology) in granulosa cells were classified as atretic. Based on histology, the atretic primary and secondary follicles (%) were 93.82 and 95.62 respectively. The histology study reveals that the rates (%) of atresia in <1, 1-3, 3-5 mm and >5 mm were 36.90, 40.50, 62.84 and 74.5 respectively. Further the atretic tertiary follicles (%) were significantly lower than the primary and secondary classes of follicles. TUNEL assay reveals that the atretic rate (%) of tertiary follicles in <1, 1-3, 3-5 and ≥ 5 mm class follicles were 50.88, 53.84, 81.81 and 36.36 respectively. The percentage of atresia in >5 mm diameter follicles were significantly lower in TUNEL than histology. Percentages of granulosa and thecal cells positive for atresia by TUNEL were 30.7 ± 0.53 and 13.82 ± 0.18 respectively per follicle. The initial structural changes in atretic follicles were seen primarily in the granulosa cells. In severely atretic follicles TUNEL positive granulosa cells along with theca cells have to be considered in assessing the rate and extent of atresia.

Changes in luteal cells distribution, apoptotic rate, lipid peroxidation levels and antioxidant enzyme activities in buffalo (Bubalus bubalis) corpus luteum.

Buffalo (Bubalus bubalis) is known for its weak/silent estrous behaviour, lower conception rate and longer inter-calving interval as compared to cattle. Understanding the kinetics and functional properties of luteal cells may be helpful to improve reproductive efficiency in the buffalo. Hence the present study was designed to assess the size and distribution of steroidogenic luteal cells along with biochemical properties during different phases of corpus luteum (CL) in the buffalo. The ovaries collected from the local abattoir were classified into three phases, early, mid and late, based on the morphological appearance of the CL as well as the follicles in the ovary. The proportion (%) of the luteal cells (>10microm diameter) increased (P<0.01) from early (30.7+/-1.3) to mid (36.30+/-1.6), and then decreased (P<0.01) in late luteal (31.46+/-1.8) phases. Percentage of small luteal cells (10-20microm diameter) was higher (P<0.05) in early (58.47+/-0.61) and mid (61.29+/-0.67) than late luteal (37.18+/-1.50) phases of CL. However, the percentage of large luteal cells (20-50microm diameter) was higher (P<0.05) only in late (62.82+/-1.50) than early (41.53+/-0.61) and mid (38.71+/-0.67) phases of CL. The average size (microm) of the large luteal cells increased (P<0.05) from early (25.46+/-0.62) to mid (27.15+/-0.5) and late (28.86+/-0.47) luteal phases. The percentage of luteal cells expressing in situ DNA fragmentation was significantly (P<0.05) higher in the late luteal (41.17+/-5.8) than mid-luteal (21.15+/-4.9) phase of the CL. In the early stage, half of the steroidogenic luteal cells had significantly (P<0.05) less 3beta-HSD activity than the other two phases. In the mid stage, the steroidogenic luteal cells had significantly higher (P<0.05) intense 3beta-HSD activity than the other two phases. Further in the late phase, a significant (P<0.05) reduction in intense 3beta-HSD activity was observed in the large luteal cells. The lipid peroxidation (micromol/g of CL) levels were significantly (P<0.05) higher in late luteal (3.46+/-0.2) than the mid-luteal (1.43+/-0.16) phases. The superoxide dismutase and catalase enzyme levels (U/mg of protein) were also significantly (P<0.05) higher in late luteal (0.9+/-0.015 and 3.37+/-0.45, respectively) than the mid-luteal (0.1+/-0.01 and 2.34+/-0.3, respectively) phases. In contrast, the GPx activity (U/mg of protein) decreased significantly (P<0.05) from mid-luteal (1.85+/-0.4) to late luteal (1.22+/-0.2) phases. The present study suggests that (i) the decrease in progesterone levels in late CL may be associated with loss of 3beta-HSD activity in large luteal cells and (ii) demise of the buffalo CL may be mediated by apoptosis despite the high levels of luteal antioxidant enzymes.

Improvement in buffalo (Bubalus bubalis) spermatozoa functional parameters and fertility in vitro: Effect of insulin-like growth factor-I.

The aim of the current study was to assess the effect of insulin-like growth factor-I (IGF-I; 100 ng/mL) on buffalo (Bubalus Bubalis) sperm functional parameters related to in vitro fertilization. The acrosin activity (the mean diameter of halo formation in micrometers) was significantly higher in the IGF-I group (14.17 +/- 1.51) compared with that in the control group (9.50+/-0.36) at 2h incubation. The mitochondrial membrane potential (per cent) was significantly higher in the IGF-I group compared with that in the control group at 30min (33.27+/-2.62 vs. 26.71+/-1.02), 60min (24.24+/-3.45 vs. 18.77+/-2.09), and 90min (22.86+/-3.02 vs. 16.92+/-1.24) incubation. The percentage of spermatozoa positive for sperm nuclear chromatin decondensation (NCD) differed significantly between the groups at 90 and 120min incubation. The comet length was significantly lower in the IGF-I group compared with that in the control group at 2h incubation. The percentage of fragmented DNA in the tail did not differ significantly between the groups at 2h incubation. The percentage of acrosomal-reacted spermatozoa did not differ significantly between the IGF-I and the control groups at 4h (41.12+/-6.44 vs. 43.53+/-5.05) incubation. The cleavage rate (per cent) was significantly higher in the IGF-I-treated group (56.73+/-3.70) compared with that in the control group (44.85+/-2.15). The current study suggests that the addition of IGF-I prevents deterioration of sperm functional parameters and fertility.

A note on fractal dimensions of biomedical waveforms.

In this paper, we study performance of Katz method of computing fractal dimension of waveforms, and its estimation accuracy is compared with Higuchi's method. The study is performed on four synthetic parametric fractal waveforms for which true fractal dimensions can be calculated, and real sleep electroencephalogram. The dependence of Katz's fractal dimension on amplitude, frequency and sampling frequency of waveforms is noted. Even though the Higuchi's method has given more accurate estimation of fractal dimensions, the study suggests that the results of Katz's based fractal dimension analysis of biomedical waveforms have to be carefully interpreted.

Complexity analysis of EEG in patients with schizophrenia using fractal dimension.

We computed Higuchi's fractal dimension (FD) of resting, eyes closed EEG recorded from 30 scalp locations in 18 male neuroleptic-naïve, recent-onset schizophrenia (NRS) subjects and 15 male healthy control (HC) subjects, who were group-matched for age. Schizophrenia patients showed a diffuse reduction of FD except in the bilateral temporal and occipital regions, with the reduction being most prominent bifrontally. The positive symptom (PS) schizophrenia subjects showed FD values similar to or even higher than HC in the bilateral temporo-occipital regions, along with a co-existent bifrontal FD reduction as noted in the overall sample of NRS. In contrast, this increase in FD values in the bilateral temporo-occipital region was absent in the negative symptom (NS) subgroup. The regional differences in complexity suggested by these findings may reflect the aberrant brain dynamics underlying the pathophysiology of schizophrenia and its symptom dimensions. Higuchi's method of measuring FD directly in the time domain provides an alternative for the more computationally intensive nonlinear methods of estimating EEG complexity.