Molecular identification and eco-friendly management of rice brown planthoppers in Bangladesh.

Infestation by various insect pests is the main constraint for growing rice where rice brown planthopper (Nilaparvata lugens Stål) can severely damage rice plants directly through feeding. Therefore, the study aims to detect rice brown planthoppers (BPH) and provide environment-friendly management tactics to mitigate the problem which caused by brown planthoppers. The BPH samples were collected from rice fields of different locations in the Patuakhali of Bangladesh for molecular identification. A molecularly single species of rice brown planthopper, Nilaparavata lugens was identified using mitochondrial cytochrome oxidase subunit I (mtCOI) universal marker. The nucleotide sequences of collected samples were compared with other nucleotide sequences from the GenBank database of NCBI, which make single clades in the phylogenetic tree at an insignificant distance. Moreover, brown planthopper management observations were recorded in laboratory conditions after providing an artificial diet with different treatments of plant-based insecticides Neem oil (1 %, 5 %, and 10 %), Castor oil (1 %, 5 %, and 10 %) where only 20 % sucrose solution was used as negative control and Abamectin (1 %, 5 % and 10 %) were also used as a positive control for comparing the efficacy of plant-based insecticides on rice brown planthoppers. The results showed the highest mortality (100 %) of rice brown planthoppers was recorded by Abamectin 10 %, followed by Abamectin 5 %. Neem 10 % performed better than Abamectin 1 % during 1st hour. Initial after exposure of 2nd hour for Abamectin 1 % revealed greater mortality (59 %) than Neem 10 %. Neem 5 % showed less effect on mortality in brown planthopper than Neem 10 % but was higher than Neem 1 % during 6 h of observation. The Castor oil of 10 % caused higher mortality than the Castor of 5 % but not up to the marks of Abamectin and different concentrations of Neem oil. Castor oil of 1 % and control have shown no mortality of brown planthopper for 6 h of observation.

Developing a tablet-based brain-computer interface and robotic prototype for upper limb rehabilitation.

Background: The current study explores the integration of a motor imagery (MI)-based BCI system with robotic rehabilitation designed for upper limb function recovery in stroke patients. Methods: We developed a tablet deployable BCI control of the virtual iTbot for ease of use. Twelve right-handed healthy adults participated in this study, which involved a novel BCI training approach incorporating tactile vibration stimulation during MI tasks. The experiment utilized EEG signals captured via a gel-free cap, processed through various stages including signal verification, training, and testing. The training involved MI tasks with concurrent vibrotactile stimulation, utilizing common spatial pattern (CSP) training and linear discriminant analysis (LDA) for signal classification. The testing stage introduced a real-time feedback system and a virtual game environment where participants controlled a virtual iTbot robot. Results: Results showed varying accuracies in motor intention detection across participants, with an average true positive rate of 63.33% in classifying MI signals. Discussion: The study highlights the potential of MI-based BCI in robotic rehabilitation, particularly in terms of engagement and personalization. The findings underscore the feasibility of BCI technology in rehabilitation and its potential use for stroke survivors with upper limb dysfunctions.

Homogeneous Catalysis in N-formylation/N-methylation Utilizing Carbon Dioxide as the C1 Source.

The growing emphasis on sustainable chemistry has driven research into utilizing carbon dioxide (CO2) as a nontoxic, abundant, and cost-effective C1 building block. CO2 offers a promising avenue for direct conversion into valuable chemicals ranging from fuels to pharmaceuticals. This review focuses on the utilization of CO2 for reductive N-formylation/N-methylation reactions of various amines, providing advantages over conventional methods involving toxic CO and other methylating reagents. The approach employs readily available reductants such as silane, borane reagents, and hydrogen (H2). The discussion encompasses recent developments in transition metal and organocatalyst systems for these reactions, highlighting mechanistic interpretations and factors influencing product selectivity.

Colored Radiative Cooling and Flame-Retardant Polyurethane-Based Coatings: Selective Absorption/Reflection in Solar Waveband.

The aesthetic demand has become an imperative challenge to advance the practical and commercial application of daytime radiative cooling technology toward mitigating climate change. Meanwhile, the application of radiative cooling materials usually focuses on the building surface, related tightly to fire safety. Herein, the absorption and reflection spectra of organic and inorganic colorants are first compared in solar waveband, finding that iron oxides have higher reflectivity in NIR region. Second, three kinds of iron oxides-based colorants are selected to combine porous structure and silicon-modified ammonium polyphosphate (Si-APP) to engineer colored polyurethane-based (PU) coating, thus enhancing the reflectivity and flame retardancy. Together with reflectivity of more than 90% in near-infrared waveband and infrared emissivity of ≈91%, average temperature drops of ≈5.7, ≈7.9, and ≈3.8 °C are achieved in porous PU/Fe2O3/Si-APP, porous PU/Fe2O3·H2O/Si-APP, and porous PU/Fe3O4·H2O/Si-APP, compared with dense control samples. The catalysis effect of iron oxides in the cross-linking reaction of pyrolysis products and dehydration mechanism of Si-APP enable PU coating to produce an intumescent and protective char residue. Consequently, PU composite coatings demonstrate desirable fire safety. The ingenious choice of colorants effectively minimizes the solar heating effect and trades off the daytime radiative cooling and aesthetic appearance requirement.

Dengue and leptospirosis coinfection: a case series.

BACKGROUND: Both dengue and Leptospira infections are endemic to tropical and subtropical regions, with their prevalence increasing in recent decades. Coinfection with these pathogens presents significant diagnostic challenges for clinicians due to overlapping clinical manifestations and laboratory findings. This case report aims to elucidate two clinical scenarios where the coinfection of dengue and leptospirosis complicates the disease course, creating a diagnostic conundrum. CASE PRESENTATION: We present the clinical scenarios of two Bangladeshi males, aged 25 and 35 years, who were admitted to our hospital with acute febrile illness. The first patient exhibited hepatic and renal involvement, while the second presented with symptoms initially suggestive of meningoencephalitis. Both cases were initially managed under the presumption of dengue infection based on positive serology. However, further evaluation revealed coinfection with Leptospira, complicating the disease course. Both patients received appropriate treatment for dengue and antibacterial therapy for leptospirosis, ultimately resulting in their recovery. CONCLUSION: These case scenarios underscore the critical importance for clinicians in regions where dengue and Leptospira are endemic to consider both diseases when evaluating patients presenting with acute febrile illness.

Perceptions and attitudes of nurse practitioners toward artificial intelligence adoption in health care.

Background: With the ever-increasing integration of artificial intelligence (AI) into health care, it becomes imperative to gain an in-depth understanding of how health care professionals, specifically nurse practitioners, perceive and approach this transformative technology. Objectives: This study aimed to gain insights into nurse practitioners' perceptions and attitudes toward AI adoption in health care. Methods: This qualitative research employed a descriptive and phenomenological approach using in-depth interviews. Data were collected through a semi-structured questionnaire with 37 nurse practitioners selected through purposive sampling, specifically Maximum Variation Sampling and Expert Sampling techniques, to ensure diversity in characteristics. Trustworthiness of the research was maintained through member checking and peer debriefing. Thematic analysis was employed to uncover recurring themes and patterns in the data. Results: The thematic analysis revealed nine main themes that encapsulated nurse practitioners' perceptions and attitudes toward AI adoption in health care. These included nurse practitioners' perceptions of AI implementation, attitudes toward AI adoption, patient-centered care and AI, quality of health care delivery and AI, ethical and regulatory aspects of AI, education and training needs, collaboration and interdisciplinary relationships, obstacles in integrating AI, and AI and health care policy. While this study found that nurse practitioners held a wide range of perspectives, with many viewings AI as a tool to enhance patient care. Conclusions: This research provides a valuable contribution to the evolving discourse surrounding AI adoption in health care. The findings underscore the necessity for comprehensive education and training in AI, accompanied by clear and robust ethical and regulatory guidelines to ensure the responsible integration of AI in health care practice. Furthermore, fostering collaboration and interdisciplinary relationships is pivotal for the successful incorporation of AI in health care. Policymakers should also address the challenges and opportunities that AI presents in the health care sector. This study enhances the ongoing conversation on AI adoption in health care by shedding light on the perspectives of nurses, thereby shaping future strategies for AI integration.

Risk factors of children's low birth weight and infant mortality in Bangladesh: Evidence from binary logistic regression and Cox PH models.

Background: Low birth weight is recognized as a pivotal risk factor affecting child survival and growth. Although Bangladesh has made commendable progress in public health, an infant mortality rate of 38 per 1000 live births and a 16% prevalence of low birth weight remain significant concerns compared to other developing countries. This situation poses a significant challenge for the formulation of future health policies in Bangladesh. As a result, this study aims to identify potential risk factors contributing to low birth weight and infant mortality among children in Bangladesh. Methods: The data is extracted from the 2014 Bangladesh Demographic and Health Survey. The response variables are infant mortality and low birth weight. In the bivariate analysis, Log-rank tests and Chi-square tests of independence were conducted. Cox proportional hazards and binary logistic regression models were utilized to determine the impact of risk factors on infant mortality and low birth weight. Results: This study identified several significant factors associated with children's low birth weight, including wealth index, parental education, birth order, twin births, mother's body mass index, and child sex. Additionally, wealth index, parental education, twin status, media exposure, birth order, antenatal care visits, prenatal care assistance, and low birth weight were identified as potential risk factors for infant mortality in Bangladesh. Conclusion: This study revealed that maternal and child characteristics, along with knowledge about child health care during pregnancy, can potentially reduce the risk of low birth weight and infant mortality among children in Bangladesh. To improve child health and survival, policymakers should prioritize community-based health education programs, and encourage parents to seek healthcare information from institutional medical facilities during pregnancy and after birth.

Why COVID-19 Symptomatic Patients Did Not Seek Healthcare Service at the Early Phase of the Pandemic in Bangladesh: Evidence From a Cross-Sectional Study.

Objective The health-seeking behavior (HSB) of patients during an outbreak is crucial in mitigating the spread of disease. Poor HSB can increase mortality and make contact tracing more difficult. In this study, we aimed to examine the status of HSB among Bangladeshi educated individuals during the early phase of the COVID-19 pandemic when infection was spreading quickly, and social distancing measures were tightened across the country. Methods We conducted a cross-sectional survey online among Bangladeshi individuals using a virtual snowball sampling method to capture suspected COVID-19 patients who did not undergo COVID-19 diagnostic testing. Descriptive and inferential analyses were performed with statistical significance defined as p<0.05. Results The study consisted of 390 participants with 44.9% having a bachelor's degree, followed by 25.9% with a master's or PhD degree. Commonly reported symptoms among the participants included fever (77.7%), cough (50.5%), headache (46.2%), body pain (36.4%), sore throat (35.6%), anosmia (31.3%), anorexia (13.8%), diarrhea (11.4%), and dyspnea (11.3%). The most common reasons for not taking the COVID-19 test were limited testing facilities (48%), the risk of infection from the test center (46%), fear of social stigma (19%), considering COVID-19 infection as innocuous (18%), and fear of forced quarantine (5%). In regression analysis, participants who lived in rural areas were found to be 2.5 times more likely to buy medications from nearby pharmacies. Males were more likely to self-medicate, with male participants being 3.2 times more likely than female participants to consider COVID-19 infection as harmless (AOR: 3.2, CI: 1.28-7.98). Smokers were more likely to seek help from government hotlines and to use drugs at home. Respondents with higher monthly income were less likely to fear forced quarantine (AOR: 0.27, CI: 0.4-2.02) but more likely to consider the risk of infection at the test center (AOR: 1.75, CI: 0.88-3.49). Conclusion Our study highlights that non-compliance with public health guidelines by educated people during an epidemic indicates a general lack of health literacy and distrust in the healthcare system. Along with improved infrastructure, efforts to enhance public health risk communication and health literacy are necessary to rebuild public trust in the healthcare service.

Salinity negatively correlates with the production and immunity of chicken: A molecular insight for food security and safety issues.

Salinity intrusion into the freshwater system due to climate change and anthropogenic activities is a growing global concern, which has made humans and domesticated animals more susceptible to diseases, resulting in less productivity. However, the effects of salinity on domesticated and wild birds, especially in terms of production and immunity, have not been fully elucidated yet. Therefore, this study was designed to examine the effects of salinity on the production and immunity of birds and the mechanisms by which immunity is compromised. Broiler chicks were subjected to different concentrations of salty water (control = normal water, treatment = 5 g/L, treatment = 10 g/L, and treatment = 15 g/L). The collected blood and organs from different groups of broilers were biochemically and histopathologically examined. Birds in salt-treated groups consumed significantly less feed than the control group, while the feed conversion ratio (FCR) was significantly higher. Body weight gain was significantly lower in salt-treated groups compared to control. Serum analysis revealed a lower systemic antibody titer in the salt-treated groups compared to the control. Primary lymphoid organs (thymus and bursa of Fabricius) were reduced in size in the salt-treated group due to cellular migration and depletion from these organs. Importantly, most of the parenchyma of lymphoid organs was replaced with fibrotic tissue. Gut microbes, Escherichia coli (E. coli) and Salmonella spp., from salt-treated groups, showed less viability but developed antibiotic resistance. Levels of salinity were significantly and negatively correlated with feed intake, body weight gain, antibody titer, lymphoid organ size, and viable count of gut microbes, while FCR, fibrosis of lymphoid organs, and antibiotic resistance were significant positively correlated. In conclusion, increased salinity is a possible threat to food security and safety as it decreases body weight gain, reduces immunity, and influences the development of multi-drug resistance in gut microbes.

Overview of Radar-Based Gait Parameter Estimation Techniques for Fall Risk Assessment.

Current methods for fall risk assessment rely on Quantitative Gait Analysis (QGA) using costly optical tracking systems, which are often only available at specialized laboratories that may not be easily accessible to rural communities. Radar placed in a home or assisted living facility can acquire continuous ambulatory recordings over extended durations of a subject's natural gait and activity. Thus, radar-based QGA has the potential to capture day-to-day variations in gait, is time efficient and removes the burden for the subject to come to a clinic, providing a more realistic picture of older adults' mobility. Although there has been research on gait-related health monitoring, most of this work focuses on classification-based methods, while only a few consider gait parameter estimation. On the one hand, metrics that are accurately and easily computable from radar data have not been demonstrated to have an established correlation with fall risk or other medical conditions; on the other hand, the accuracy of radar-based estimates of gait parameters that are well-accepted by the medical community as indicators of fall risk have not been adequately validated. This paper provides an overview of emerging radar-based techniques for gait parameter estimation, especially with emphasis on those relevant to fall risk. A pilot study that compares the accuracy of estimating gait parameters from different radar data representations - in particular, the micro-Doppler signature and skeletal point estimates - is conducted based on validation against an 8-camera, marker-based optical tracking system. The results of pilot study are discussed to assess the current state-of-the-art in radar-based QGA and potential directions for future research that can improve radar-based gait parameter estimation accuracy.

Profiling of antioxidant properties and identification of potential analgesic inhibitory activities of Allophylus villosus and Mycetia sinensis employing in vivo, in vitro, and computational techniques.

ETHNOPHARMACOLOGICAL RELEVANCE: The traditional use of plants for medicinal purposes, called phytomedicine, has been known to provide relief from pain. In Bangladesh, the Chakma indigenous community has been using Allophylus villosus and Mycetia sinensis to treat various types of pain and inflammation. AIM OF THE STUDY: The object of this research is to evaluate the effectiveness of these plants in relieving pain and their antioxidant properties using various approaches such as in vitro, in vivo, and computational techniques. Additionally, the investigation will also analyse the phytochemicals present in these plants. MATERIALS AND METHODS: We conducted in vivo analgesic experiment on Swiss albino mice and in-silico inhibitory activities on COX-2 & 15-LOX-2 enzymes. Assessment of DPPH, Anti Radical Activities (ARA), FRAP, H2O2 Free Radical Scavenging, Reducing the power of both plants performed significant % inhibition with tolerable IC50. Qualitative screening of functional groups of phytochemicals was précised by FTIR and GC-MS analysis demonstrated phytochemical investigations. RESULTS: The ethyl acetate (EtOAc) fractioned Mycetia sinensis extract as well as the ethanoic extract and all fractioned extracts of Allophylus villosus have reported a significant percentage (%) of writhing inhibition (p < 0.05) with the concentrated doses 250 mg as well as 500 mg among the Swiss albino mice for writhing observation of analgesic effect. In the silico observation, a molecular-docking investigation has performed according to GC-MS generated 43 phyto-compounds of both plants to screen their binding affinity by targeting COX-2 and 15-LOX-2 enzymes. Consequently, in order to assess and ascertain the effectiveness of the sorted phytocompounds, ADMET (Absorption, Distribution, Metabolism, Excretion, and Toxicity) investigation, DFT (Density-functional theory) by QM (Quantum mechanics), and MDS (Molecular dynamics simulation) were carried out. As the outcome, compounds like 5-(2,4-ditert-butylphenoxy)-5-oxopentanoic acid; 2,4-ditert-butylphenyl 5-hydroxypentanoate; 3,3-diphenyl-5-methyl-3H-pyrazole; 2-O-(6-methylheptan-2-yl) 1-O-octyl benzene-1,2-dicarboxylate and dioctan-3-yl benzene-1,2-dicarboxylate derived from the ethnic plant A. villosus and another ethnic plant M. sinensis extracts enchants magnificent analgesic inhibitions and performed more significant drug like activities with the targeted enzymes. CONCLUSIONS: Phytocompounds from A. villosus & M. sinensis exhibited potential antagonist activity against human 15-lipoxygenase-2 and cyclooxygenase-2 proteins. The effective ester compounds from these plants performed more potential anti-nociceptive activity which could be used as a drug in future.

Assessing the efficacy of date-pits holocellulose as a novel additive candidate for ruminant feeding.

Holocellulose (HC) fraction extracted from date-pits was evaluated as a novel feed additive for ruminant feeding. This study was performed to investigate the effectiveness of the HC additive on rumen fermentation, methane (CH4) production, and diet degradability over 24 h of in vitro incubation. Three independent incubation trials were conducted over three consecutive weeks, employing the same in vitro methodology to assess four treatment doses in a completely randomized design. The experimental diet incorporated four increasing doses of HC, containing HC at 0 (HC0), 10 (HC10), 20 (HC20), and 30 (HC30) g/kg dry matter (DM). In vitro gas production (GP) and CH4 production, volatile fatty acids (VFAs) concentration, protozoa accounts, degraded organic matter (DOM), metabolizable and net energy (ME and NE), and hydrogen (H2) estimates were measured. No significant differences in ruminal pH were observed as the HC doses gradually increased. All incremental doses of HC additive over 24 h resulted in a linear increase in GP (P < 0.001), DOM (P < 0.001), total VFAs (P = 0.011), and propionate (P < 0.001) concentrations, as well as estimated energy (ME and NE) (P < 0.05) and microbial protein (P = 0.017) values. However, the inclusion of increasing doses of HC in the diet displayed linear reductions in the net CH4 production (ml/kg DOM; P = 0.002), protozoa abundance (P = 0.027); acetate (P = 0.029), and butyrate (P < 0.001) concentrations, the acetate-to-propionate ratio (P < 0.001), and the estimated net H2 production concentration (P = 0.049). Thus, the use of date-pits HC additive generated positive ruminal fermentability, including increased total VFAs and a reduction in the acetate-to-propionate ratio, leading to decreased CH4 output over 24 h of in vitro incubation. Hence, HC could be considered a potent feed additive (at up to 30 g/kg DM), demonstrating promising CH4-mitigating competency and thereby enhancing energy-use efficiency in ruminants.

A Bibliometric Analysis of the 100 Most Cited Articles on Otoplasty.

A bibliometric analysis was conducted in April 2024, to review the current trends in otoplasty. It involved literature search on Scopus database for original articles using the query term "otoplasty" and "pinnaplasty", without restricting publication dates or selecting journals in the database. The top 100 articles with the highest citations were reviewed. Bibliometric analysis was performed using Scimago journal impact factor. The screening was done using the Preferred Reporting Items for Systematic Reviews and MetaAnalyses (PRISMA) guidelines to extract the top 100 most cited articles in otoplasty. We excluded articles not focused on otoplasty, those involving other surgical procedures, and non-English articles. Scopus and manual screening were used together to perform citation analysis of selected articles. Mean values were calculated for the number of citations and citations per year. Of the 951 studies identified, 100 (10.5%) were analyzed in detail. The studies were published between 1941-2024, with a mean of 36 ± 23.7 citations per paper. The journal "Plastic and Reconstructive Surgery" reported the highest number (23[23%]) of articles, followed by "Journal of Plastic, Reconstructive and Aesthetic Surgery" with 13% (n=13) articles. The highest number of articles originated from the United States (38%, n=38), followed by United Kingdom (15%, n=15). Our bibliometric analysis provides a comprehensive overview of the landscape of otoplasty research, highlighting key publications, authors, and journals. This study contributes to the understanding of the evolution and impact of otoplasty literature, laying the groundwork for further research and innovation in this field.

Plasma metabolomic profile of adiposity and body composition in childhood: The Genetics of Glucose regulation in Gestation and Growth cohort.

OBJECTIVE: This study identified metabolite modules associated with adiposity and body fat distribution in childhood using gold-standard measurements. METHODS: We used cross-sectional data from 329 children at mid-childhood (age 5.3 ± 0.3 years; BMI 15.7 ± 1.5 kg/m2) from the Genetics of Glucose regulation in Gestation and Growth (Gen3G), a prospective pre-birth cohort. We quantified 1038 plasma metabolites and measured body composition using the gold-standard dual-energy x-ray absorptiometry (DXA), in addition to skinfold, waist circumference, and BMI. We applied weighted-correlation network analysis to identify a network of highly correlated metabolite modules. Spearman's partial correlations were applied to determine the associations of adiposity with metabolite modules and individual metabolites with false discovery rate (FDR) correction. RESULTS: We identified a 'green' module of 120 metabolites, primarily comprised of lipids (mostly sphingomyelins and phosphatidylcholine), that showed positive correlations (all FDR p < 0.05) with DXA estimates of total and truncal fat (ρadjusted = 0.11-0.19), skinfold measures (ρadjusted = 0.09-0.26), and BMI and waist circumference (ρadjusted = 0.15 and 0.18, respectively). These correlations were similar when stratified by sex. Within this module, sphingomyelin (d18:2/14:0, d18:1/14:1)*, a sphingomyelin sub-specie that is an important component of cell membranes, showed the strongest associations. CONCLUSIONS: A module of metabolites was associated with adiposity measures in childhood.

Adverse childhood experiences and externalizing, internalizing, and prosocial behaviors in children and adolescents: A longitudinal study.

BACKGROUND: Adverse childhood experiences (ACEs) have a profound impact on individuals, shaping their long-term health and life opportunities. This study delves into the complex ties between ACEs and the socioemotional development of Australian children and youth by examining the relationships between adverse childhood experiences and externalizing, internalizing, and prosocial behaviors. METHODS: This study utilized data from the Longitudinal Study of Australian Children and employed the generalized estimating equation method to investigate the relationships between adverse childhood experiences and externalizing, internalizing, and prosocial behaviors in Australian children and adolescents. RESULTS: Adverse childhood experiences such as physical punishment, hostile parenting, parental conflicts, separation, financial strain, and parental mental health issues increased the risk of externalizing and internalizing behaviors while reducing prosocial behaviors. The risk increases with the number of ACEs, as evidenced by the incidence ratio (IR); for example, for externalizing behaviors, an ACEs score of one leads to IR = 1.69, while an ACEs score of 4 results in IR = 3.34. Similar trends were observed for internalizing and prosocial behaviors. LIMITATIONS: The presence of imbalanced longitudinal data, arising from variations in the number of observations across different time points, challenges robust inferences. Furthermore, this study investigates the relationship between ACEs and behavioral problems, without establishing causality. Consequently, the results should be interpreted with caution. CONCLUSIONS: The findings of this study highlight that adverse childhood experiences significantly influence behavioral outcomes in children and adolescents. These findings underscore the critical need for early detection and intervention to mitigate the consequences of traumatic childhood experiences.

Renewable energy and ecological footprint nexus: Evidence from dynamic panel threshold technique.

The escalating phenomenon of environmental degradation is an urgent global concern, imperiling ecosystems and hindering the prospects for sustainable development on a planetary scale. Therefore, this study aims to explore the intricate interplay between renewable energy (RE) and ecological footprint (EF), considering the conditional impact of fiscal capacity (FIC), human development (HDI), institutional quality (IQI), and population density (PDN). Drawing on panel data encompassing 74 developing countries from 2000 to 2022, the study employs a dynamic panel threshold regression method, both with and without an instrumental variable approach. The findings unveil a non-linear nexus between RE and EF, revealing significant threshold values for FIC (1.870), HDI (0.736), and IQI (0.311), above which RE showcases its efficacy in mitigating EF. Conversely, when these predictors dip below the thresholds of FIC (1.391), HDI (0.655), and IQI (0.2545), the impact of RE on FE becomes insignificant. Moreover, the study introduces PDN as an additional threshold variable in the analysis, pinpointing that the effectiveness of RE in reducing EF hinges on PDN being below a threshold value of 263.144; however, above a threshold value of 276.98, the influence of PDN on the RE-FE nexus diminishes. The findings underscore the complexity of policy landscapes in developing countries. They suggest that while promoting renewable energy is pivotal for environmental sustainability, it is equally imperative to bolster existing environmentally friendly fiscal capacity, advance human capital, enhance institutional quality, and craft effective population distribution policies.

Fertilizer management practices in potato cultivation: a baseline study for the introduction of GE potato in Bangladesh.

Genetically engineered (GE) crops have the potential to contribute to agricultural sustainability, food security, and nutritional enrichment. However, these crops cannot be released for commercial cultivation without undergoing environmental risk assessments (ERA), thus biosafety evaluation. ERA assessments are performed comparatively with their natural non-GE counterparts. As Bangladesh is progressing with GE potato research, the present study aims to collect baseline information on non-GE potato cultivation with an emphasis on current agronomic practices focusing on fertilizer management and farmers' knowledge base. The survey had three parts, including information on the farmers, information on potato cultivation practices, especially fertilizer use, and lastly, the farmer's view on GE potato. From 2020 to 2021, data were collected through interviews with experienced growers in four potato-growing regions, the Central and Mid-East, North-West, Mid-West, and South-East regions (n = 1757) of the country. The study revealed that farmers of all regions used more than the recommended amounts of fertilizer; for instance, 67.1% more nitrogen fertilizer was applied as an extra dose during potato cultivation in Munshiganj (Central and Mid-East) than in the Dinajpur region (North-West). This overuse of nitrogen fertilizer can enhance plant vigor but makes the plants more susceptible to insect attraction and allows pests easier access to the plants. As a result, the excess dose of nitrogen fertilizer in Munshiganj may act as a catalyst to increase the probability of late blight. The findings also showed that 73.6% of farmers observed unexpected flowering in certain potato cultivars, which corresponded to the higher application of phosphate and potassium fertilizers aimed at late blight control. Furthermore, this study reported infestations of Solanaceous weeds, specifically Solanum torvum and Physalis heterophylla, in potato fields. Finally, our findings demonstrated that more than 68.7% of the potato growers intend to adopt disease-resistant GE potato as that may reduce the need for excess fertilizer use and thus reduce cultivation costs.

Mental health status among chronic disease patients in Bangladesh during the COVID-19 Pandemic: Findings from a cross-sectional study.

Context: The COVID-19 epidemic has had a substantial influence on the mental health of chronic disease patients. However, there is a scarcity of research on them in Bangladesh. Aims: This study aims to explore the prevalence of and identify the risk factors for depression, anxiety, and stress symptoms during the COVID-19 pandemic among people with chronic diseases in Bangladesh. Materials and Methods: This cross-sectional study involving face-to-face and telephone interviews was carried out among Bangladeshi people diagnosed with chronic diseases between September and November 2020. The total sample size was 878, and a convenient sampling technique was used. Logistic regression analysis was performed to investigate potential influencing factors for depression, anxiety, and stress. Results: The mean age of respondents was 50.10 years. Among them, 35.0%, 36.0%, and 29.0% suffered from depression, anxiety, and stress symptoms, respectively. In multivariable logistic regression, depression had a significant positive association with higher age (≥60 years), lower income, rural residency, and loss of close family members due to COVID-19. Anxiety had a significant positive association with higher age (≥40 years), lower education, lower income, rural residency, and loss of close family members due to COVID-19. Stress had a significant positive association with higher age (≥40 years), no income, rural residency, and loss of close family members due to COVID-19. Conclusion: It is urgent to consider the risk of developing mental health distress among chronic disease patients, especially aged people, by health service providers and generate effective programs for emergency situations.

Comprehensive analysis of phytochemical profiling, cytotoxic and antioxidant potentials, and identification of bioactive constituents in methanoic extracts of Sonneratia apetala fruit.

In the delta region of Bangladesh, Sonneratia apetala, also known as Keora and mangrove apple, is widely recognized for its dual role as a source of both food and medicine. Seasonal S. apetala fruits were gathered from Hatiya, Noakhali, in October 2021. The samples were segregated into pericarps and seeds, then fractionated into methanol segments. The anti-proliferative activities of these samples against lung A549 cells were evaluated using the Trypan blue exclusion method. Additionally, High-Performance Liquid Chromatography (HPLC) was employed to quantify phenolic compounds, while standard protocols facilitated the identification of specific phytochemical constituents. Chemical profiling via Gas Chromatography-Mass Spectrometry (GC-MS) and the isolation and detection of bioactive compounds through column chromatography and Nuclear Magnetic Resonance (NMR) analysis were undertaken. The methanol fractions of the seeds and pericarp were found to contain carbohydrates, tannins, flavonoids, steroids, alkaloids, glycosides, and terpenoids, with the absence of saponins and anthraquinones. Notably, the anti-proliferative effect demonstrated statistical significance at a concentration of 300 µg/mL for both extracts. Furthermore, HPLC analysis identified and quantified six polyphenols: catechin hydrate, (-)-epicatechin, rutin hydrate, trans-ferulic acid, trans-cinnamic acid, myricetin, and kaempferol, with the following concentrations: 46.65 and 12.72; 349.29 and 140.39; 5.26 and 33.06; 10.35 and 29.28; ND and 11.93; and 10.03 and 7.90 mg/100 g in the methanol fraction of the seed and pericarp, respectively. GC-MS analysis of S. apetala fruit revealed five notable compounds with significant peak areas (%): 2-methyltetracosane, tetratetracontane, heptacosane, 1-chloro-2-hexyl-1-octanol, and phenol, 3,5-bis(1,1-dimethylethyl), exhibiting peak areas of 43.96, 35.8, and 15.95, respectively. Meanwhile, the notable peak in S. apetala seeds was 1,3-benzenedicarboxylic acid, bis(2-ethylhexyl) ester, with a peak area (%) of 100. These compounds are known for their anticancer and antioxidant properties. Therefore, S. apetala, particularly its seeds and fruits, shows promising potential for development into dietary supplements and functional foods.